Characterization of BPA Sensitive Strains in C. elegans

Bisphenol A (BPA) has been present in our environment since 1891 when it was first synthesized by Aleksandr Dianin. BPA has since been a topic of interest due to the possibility of diseases associated in human consumption of the chemical. Interestingly, Caenorhabditiselegans, commonly known as C.eleganshave shown to be able to tolerate the chemical. Thisorganism is an advantageous model organism for research since they are simple organisms to study, cheap, produce a large brood size, and have a short life cycle. Previously a forward genetic screen was done to isolate 2000 healthy strains in order to determine which strains showed sensitivity to BPA.bin-1, a mutant strain, was isolated and was fully sequenced to determine the gene responsible for BPA sensitivity. It was found that bin-1was essential for BPA sensitivity because the BIN-1 protein caused the animals to be able to survive in the presence of BPA. The BIN-1 protein is predicted to function as a glycosylase, although this has yet to be tested. Research done by Nakajima has found that other animals such as freshwater algae and tobacco cell lines have been able to glycosylate BPA(Nakajima et al. 2004 and Nakajima et al. 2007). Therefore, my research is centered upon (1) characterizing BPA-sensitive strains found in a forward genetic screen and (2) prioritizing which strains are most sensitive to BPA. This was performed through daily sensitivity tests. I expected to find some strains to show sensitivity and some do not. With the results of my research, I will be able to determine which strains showed increased sensitivity and need to be further tested to see why they are showing sensitivity to BPA

L'esplendor corporatiu en les ciutats catalanes del segle XVI. L'exemple de Lleida

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Hisenda municipal i arrendaments públics al Balaguer del segle XVIII

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Tradició, civisme i moralitat pública a la Lleida del segle XVI. Les ordinacions municipals de 1559

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Primera cita de Mauremys leprosa (Sauropsida: Testudines) a Sant Mateu del Maestrat

La tortuga de rierol, Mauremys leprosa (Scheigger, 1812), és citada per primer cop a Sant Mateu del Maestrat (el Baix Maestrat). Es fa una primera valoració de l'estat de la població, que es considera bona, amb una estructura d'edat normal i amb abundants exemplars juvenils que asseguren el reemplaçament. No s'han observat altres torugues d'aigua a la zona.First record of the Mauremys leprosa (Sauropsida: Testudines) in Sant Mateu del Maestrat.Terrapin, Mauremys leprosa (Schegger, 1812), is mentioned for the first time in Sant Mateu del Maestrat (el Baix Maestrat, Spain). A first assessment of he state of the population is made. That is considered good, with a natural age structure and abundant young speciments that ensure replacement. No other turtles were observed in the are

El Compostatge com a sistema de tractament de residus: compostatge de residus sòlids urbans - fangs de depuradores

L'augment de diferents residus orgànics i la problemàtica de la conservació del medi ambient ha comportat l'estudi de tècniques d'aprofitament d'aquests residus i la seva posterior utilització. Entre una de les nombroses tècniques, hi ha el "Compostatge" que dóna com a producte final el "Compost" (utilitzable com a adob o esmena orgánica en agricultura). En aquest treball pretenem de veure com podem realitzar aquest procés amb el mínim de despeses econòmiques així com de trobar una manera adequada de treure una mostra representativa de la pila, de la durada del procés i del tractament de la mostra per tal de realitzar els anàlisis.The increase of different types of organic refuses and the problem of the conservation of the natural environment has lead to the study of the techniques that make these refuses re-utilizable. Among these techniques we find the compostage which final product is known as the compost (utilizable in agriculture as fertilizer and as organic amendmend). In this study we try to explain how this process should be done being as cheap as possible and we also try to find the better way to sample (the sample mus representative of the pile) the time the process takes and the teatment of the sample in order to be analyzed.El aumento de diferentes residuos orgánicos y la problemática de la conservación del medio ambiente ha implicado el estudio de técnicas de aprovechamiento de estos residuos y su posterior utilización. Entre las numerosas técnicas está el "Compostage" que da como producto final el "Compost" (utilizable como abono o enmienda orgánica en agricultura). En este trabajo se pretende ver como realizar este proceso con el mínimo coste, así como encontrar una forma adecuada de sacar una muestra representativa de la pila, duración del proceso y tratamiento de la muestra para realizar los análisis