Biological activities specified by antibiotic resistance plasmids

Bacteria can display resistance to a wide spectrum of noxious agents and environmental conditions, and these properties are often mediated by genes located on extrachromosomal DNA elements called plasmids. Replication, vertical and horizontal transmission and evolution of these elements are discussed, and examples of the genes responsible for the resistance phenotypes are given. Selective forces that drive the evolution of new combinations of bacterial properties of particular importance in clinical situations are analyse

Biological activities specified by antibiotic resistance plasmids

Bacteria can display resistance to a wide spectrum of noxious agents and environmental conditions, and these properties are often mediated by genes located on extrachromosomal DNA elements called plasmids. Replication, vertical and horizontal transmission and evolution of these elements are discussed, and examples of the genes responsible for the resistance phenotypes are given. Selective forces that drive the evolution of new combinations of bacterial properties of particular importance in clinical situations are analyse

Coilin interacts with Ku proteins and inhibits in vitro non-homologous DNA end joining

AbstractCoilin is a nuclear protein that plays a role in Cajal body formation. The function of nucleoplasmic coilin is unknown. Here we report that coilin interacts with Ku70 and Ku80, which are major players in the DNA repair process. Ku proteins compete with SMN and SmB′ proteins for coilin interaction sites. The binding domain on coilin for Ku proteins cannot be localized to one discrete region, and only full-length coilin is capable of inhibiting in vitro non-homologous DNA end joining (NHEJ). Since Ku proteins do not accumulate in CBs, these findings suggest that nucleoplasmic coilin participates in the regulation of DNA repair.Structured summaryMINT-8052983:coilin (uniprotkb:P38432) physically interacts (MI:0915) with SmB′ (uniprotkb:P14678) by pull down (MI:0096)MINT-8052941:coilin (uniprotkb:P38432) physically interacts (MI:0915) with Ku70 (uniprotkb:P12956) by competition binding (MI:0405)MINT-8052765:coilin (uniprotkb:P38432) physically interacts (MI:0915) with Ku80 (uniprotkb:P13010) by pull down (MI:0096)MINT-8052971:coilin (uniprotkb:P38432) physically interacts (MI:0915) with SMN (uniprotkb:Q16637) by pull down (MI:0096)MINT-8052957:coilin (uniprotkb:P38432) physically interacts (MI:0915) with Ku80 (uniprotkb:P13010) by competition binding (MI:0405)MINT-8052894, MINT-8052908:coilin (uniprotkb:P38432) binds (MI:0407) to Ku80 (uniprotkb:P13010) by pull down (MI:0096)MINT-8052804:coilin (uniprotkb:P38432) physically interacts (MI:0915) with Ku80 (uniprotkb:P13010) by anti bait coimmunoprecipitation (MI:0006)MINT-8052925:coilin (uniprotkb:P38432) binds (MI:0407) to Ku70 (uniprotkb:P12956) by pull down (MI:0096)MINT-8052786:Ku80 (uniprotkb:P13010) physically interacts (MI:0914) with coilin (uniprotkb:P38432) and Ku70 (uniprotkb:P12956) by anti bait coimmunoprecipitation (MI:0006)MINT-8052776:coilin (uniprotkb:P38432) physically interacts (MI:0915) with Ku70 (uniprotkb:P12956) by pull down (MI:0096

Explorant un Còdex del segle XIV

Un investigador del Departament d'Art i Musicologia de la UAB ha analitzat un còdex del segle XIV anomenat "Libro de la Coronación de los Reyes de Castilla", l'únic document litúrgic conservat per la coronació d'un rei castellà. L'exploració d'aquest manuscrit, que es troba a la Real Biblioteca de El Escorial, revela que aquest document és l'adaptació d'un altre manuscrit, anomenat Ordo de Constantinoble, del que, curiosament, se'n conserva un exemplar a l'Arxiu Capitular de Toledo. Finalment, el còdex també mostra que Santiago de Compostel·la va ser el lloc triat originalment per a la coronació d'Alfons XI de Castella, que finalment va ser coronat al monestir de Las Huelgas, a Burgos.Un investigador del Departamento de Arte y Musicología de la UAB ha analizado un códice del siglo XIV llamado "Libro de la Coronación de los Reyes de Castilla", el único documento litúrgico conservado sobre la coronación de un rey castellano. La exploración de este manuscrito, que se encuentra en la Real Biblioteca de El Escorial, revela que este documento es la adaptación de otro manuscrito, llamado Ordo de Constantinopla, del que, curiosamente, se conserva un ejemplar en el Archivo Capitular de Toledo. Finalmente, el códice también muestra que Santiago de Compostela fue el lugar elegido originalmente para la coronación de Alfonso XI de Castilla, que finalmente fue coronado en el monasterio de Las Huelgas, en Burgos.A researcher in the UAB's Department of Arts and Musicology has analysed a 14th-century codex called "Libro de la Coronación de los Reyes de Castilla", the only surviving liturgical document for the coronation of a Spanish king. Exploration of this manuscript, preserved in the Real Biblioteca El Escorial, reveals that it is an adaptation of another manuscript called "Ordo of Constantinople", of which, curiously, a copy is kept in the Archivo Capitular in Toledo. Finally, the codex also shows that Santiago de Compostela was the site originally chosen for the coronation of King Alfonso XI of Castile, who was actually crowned in the monastery of Las Huelgas in Burgos

Gestión del proyecto de localización del sitio web 'http : //beanengineer.com/'

Este trabajo recoge una visión general de la gestión total del proyecto de localización del sitio web , desde la descarga de los archivos hasta la edición final del formato. Con el fin de ofrecer una visión amplia del proceso de localización web y sus características, se incluye una descripción de las diferentes fases del proyectoAquest treball recull una visió general de la gestió total del projecte de localització de la pàgina web , des de la descàrrega dels arxius fins a l'edició del format. Amb la finalitat d'oferir una visió extensa del procés de localització web i les seves característiques, s'hi inclou una descripció de les diverses fases del projecteThis essay presents an overview of the complete management of the localization project of the website , from the download of the files to the final editing. With the aim of providing a wide vision of the web localization project and its features, a description of the different parts of the project is include

Mechanochemically synthesized Pb-free halide perovskite-based Cs2AgBiBr6–Cu–RGO nanocomposite for photocatalytic CO2 reduction

Pb-based halide perovskites have recently showed great potential in various applications such as solar cells, optoelectronics and photocatalysis. Despite their high performance, the Pb2+ toxicity along with poor stability hinders long term applications in photocatalysis. Herein, we report mechanochemically prepared Pb-free Cs2AgBiBr6 double perovskite nanoplates and their heterostructure with Cu-loaded reduced graphene oxide (Cu–RGO) for gas-phase photocatalytic CO2 reduction using water vapor as the proton source in the absence of a hole scavenger. The resulting Cs2AgBiBr6–Cu–RGO nanocomposite shows significant photocatalytic activity of 10.7 (±0.6) μmol CH4 g−1 h−1, 1.9 (±0.3) μmol CO g−1 h−1 and 1.0 (±0.2) μmol H2 g−1 h−1, with a CH4 selectivity of 93.0 (±0.5)% on an electron basis with 1 sun and a remarkable apparent quantum efficiency of 0.89 (±0.21)% at 590 nm. A further 32% enhancement in photocatalytic activity on an electron basis is achieved when the light intensity is doubled (2 suns). The high performance was attributed to their improved charge separation and suppressed electron–hole recombination, along with extended visible light absorption, better stability in a humid environment and improved CO2 adsorption. These findings support Cs2AgBiBr6 as a potential Pb-free alternative to conventional halide perovskites for photocatalytic solar-to-fuel conversion and CO2 utilization

Hybrid MSRM-Based Deep Learning and Multitemporal Sentinel 2-Based Machine Learning Algorithm Detects Near 10k Archaeological Tumuli in North-Western Iberia

This paper presents an algorithm for large-scale automatic detection of burial mounds, one of the most common types of archaeological sites globally, using LiDAR and multispectral satellite data. Although previous attempts were able to detect a good proportion of the known mounds in a given area, they still presented high numbers of false positives and low precision values. Our proposed approach combines random forest for soil classification using multitemporal multispectral Sentinel-2 data and a deep learning model using YOLOv3 on LiDAR data previously pre-processed using a multi–scale relief model. The resulting algorithm significantly improves previous attempts with a detection rate of 89.5%, an average precision of 66.75%, a recall value of 0.64 and a precision of 0.97, which allowed, with a small set of training data, the detection of 10,527 burial mounds over an area of near 30,000 km2, the largest in which such an approach has ever been applied. The open code and platforms employed to develop the algorithm allow this method to be applied anywhere LiDAR data or high-resolution digital terrain models are available

Diversidad de mariposas (Lepidoptera, Papilionoidea) y su relación con el paisaje de alta montaña en los Andes nororientales de Colombia

Diversitat de papallones (Lepidoptera, Papilionoidea) i la seva relació amb el paisatge d’alta muntanya als Andes nord-orientals de Colòmbia La regió andina es caracteritza per acollir una gran varietat d’ecosistemes entre els quals es compten les característiques zones d’alta muntanya, que mostren processos de transformació del sòl accelerats que han convertit els boscos natius en zones agrícoles i ramaderes, la qual cosa ha ocasionant un declivi de la diversitat biològica regional. L’ús d’indicadors de diversitat és adequat per avaluar els canvis en els paisatges d’alta muntanya i les papallones són un dels grups més importants per determinar els canvis que es produeixen en els diferents tipus d’ambients. A l’àrea d’estudi es van establir dotze punts de mostreig als complexos d’erms d’Almorzadero, Santurbán i Tamá de la regió nord-oriental de Colòmbia. Durant la fase de mostreig es van practicar captures d’individus adults de papallones en transsectes de 100 x 4 m en un gradient altitudinal de 500 m. Es van registrar 69 espècies, pertanyents a cinc famílies. Els valors de diversitat de la comunitat de papallones diürnes van variar a les diferents cobertures vegetals associades a cada transsecte. Així, les àrees amb cobertura vegetal conservada (per exemple, bosc dens, arbustos i matolls i erm i suberm) van presentar els valors més alts de diversitat, mentre que les zones amb cobertura alterada (per exemple, bosc fragmentat, pastures i àrees agrícoles) van presentar els valors de diversitat més baixos. Les anàlisis d’estructura de comunitats evidencien que les espècies de papallones endèmiques estan associades a àrees fragmentades a les zones d’alta muntanya. Aquest estudi és el primer del seu tipus que estableix canvis en la diversitat de les papallones en zones d’alta muntanya del nord-est de Colòmbia i s’ha fet amb el propòsit de generar estratègies de conservació en llocs que presenten un alt grau d’endemisme i diversitat.Diversity of butterflies (Lepidoptera, Papilionoidea) and their relationship with the highlands’ landscape in the northeastern Andes of Colombia The Andean region harbors a great variety of ecosystems, including the characteristic high mountain zones. The biological diversity in these zones, however, is declining as the result of changes in land use, particularly the introduction of agriculture and cattle ranching. Changes in the high mountain landscape can be appropriately monitored using diversity indicators such as butterflies, one of the most important indicator species. We chose twelve sampling points in the paramo (high altitude moor) complexes Almorzadero, Santurbán and El Tamá, located in the northeastern region of Colombia to collect adult butterflies within transects of 100 x 4 m, along an altitudinal gradient of 500 m. We recorded 69 species, from five families. Diversity values of the diurnal butterfly communities varied among  the different areas of vegetation coverage associated with each transect. Conserved vegetation coverage (i.e. dense forest, scrublands and scrublands, and paramo and subparamo) showed the highest values of diversity, while  perturbed coverage (i.e. fragmented forests, pastures and agriculture areas) presented the lowest values of diversity. Analysis of the community structure showed that endemic butterfly species were associated with fragmented areas in the high mountains. Findings from this study, the first of its kind to determine changes in butterfly diversity in highlands in northeastern Colombia, may help to generate conservation strategies in places where there is high endemism and diversityLa región andina se caracteriza por albergar una gran variedad de ecosistemas entre los que se cuentan las características zonas de alta montaña, que muestran acelerados procesos de transformación del suelo que han convertido los bosques nativos en zonas agrícolas y ganaderas ocasionando un declive de la diversidad biológica regional. El empleo de indicadores de diversidad es adecuado para evaluar los cambios en los paisajes de alta montaña, siendo las mariposas uno de los grupos más importantes para determinar los cambios que se producen en los diferentes tipos de ambientes. En el área de estudio se establecieron doce puntos de muestreo en los complejos de páramos del Almorzadero, Santurbán y Tamá de la región nororiental de Colombia. Durante la fase de muestreo se realizaron capturas de individuos adultos de mariposas en transectos de 100 x 4 m en un gradiente altitudinal de 500 m. Se registraron 69 especies, pertenecientes a cinco familias. Los valores de diversidad de la comunidad de mariposas diurnas variaron en las diferentes coberturas vegetales asociadas a cada transecto. Así, las áreas con cobertura vegetal conservada (por ejemplo, bosque denso, arbustos y matorrales y páramo y subpáramo) presentaron los valores más altos de diversidad, mientras que las zonas con cobertura alterada (por ejemplo, bosque fragmentado, pastos y áreas agrícolas) presentaron los valores de diversidad más bajos. Los análisis de estructura de comunidades evidencian que las especies de mariposas endémicas están asociadas a áreas fragmentadas en las zonas de alta montaña. Este estudio es el primero de su tipo que establece cambios en la diversidad de las mariposas en zonas de alta montaña del nordeste de Colombia y se ha realizado con el propósito de generar estrategias de conservación en lugares que presentan un alto grado de endemismo y diversidad

Separate and combined effects of individual and neighbourhood socio-economic disadvantage on health-related lifestyle risk factors:a multilevel analysis

BACKGROUND: Socio-economic disadvantage at both individual and neighbourhood levels has been found to be associated with single lifestyle risk factors. However, it is unknown to what extent their combined effects contribute to a broad lifestyle profile. We aimed to (i) investigate the associations of individual socio-economic disadvantage (ISED) and neighbourhood socio-economic disadvantage (NSED) in relation to an extended score of health-related lifestyle risk factors (lifestyle risk index); and to (ii) investigate whether NSED modified the association between ISED and the lifestyle risk index. METHODS: Of 77 244 participants [median age (IQR): 46 (40-53) years] from the Lifelines cohort study in the northern Netherlands, we calculated a lifestyle risk index by scoring the lifestyle risk factors including smoking status, alcohol consumption, diet quality, physical activity, TV-watching time and sleep time. A higher lifestyle risk index was indicative of an unhealthier lifestyle. Composite scores of ISED and NSED based on a variety of socio-economic indicators were calculated separately. Linear mixed-effect models were used to examine the association of ISED and NSED with the lifestyle risk index and to investigate whether NSED modified the association between ISED and the lifestyle risk index by including an interaction term between ISED and NSED. RESULTS: Both ISED and NSED were associated with an unhealthier lifestyle, because ISED and NSED were both positively associated with the lifestyle risk index {highest quartile [Q4] ISED beta-coefficient [95% confidence interval (CI)]: 0.64 [0.62-0.66], P < 0.001; highest quintile [Q5] NSED beta-coefficient [95% CI]: 0.17 [0.14-0.21], P < 0.001} after adjustment for age, sex and body mass index. In addition, a positive interaction was found between NSED and ISED on the lifestyle risk index (beta-coefficient 0.016, 95% CI: 0.011-0.021, Pinteraction < 0.001), which indicated that NSED modified the association between ISED and the lifestyle risk index; i.e. the gradient of the associations across all ISED quartiles (Q4 vs Q1) was steeper among participants residing in the most disadvantaged neighbourhoods compared with those who resided in the less disadvantaged neighbourhoods. CONCLUSIONS: Our findings suggest that public health initiatives addressing lifestyle-related socio-economic health differences should not only target individuals, but also consider neighbourhood factors