Draft Genome Sequence of Xanthomonas axonopodis pv. allii Strain CFBP 6369

We report here the draft genome sequence of Xanthomonas axonopodis pv. allii strain CFBP 6369, the causal agent of bacterial blight of onion. The draft genome has a size of 5,425,942 bp and a G+C content of 64.4%

NS2 Protein of Hepatitis C Virus Interacts with Structural and Non-Structural Proteins towards Virus Assembly

Growing experimental evidence indicates that, in addition to the physical virion components, the non-structural proteins of hepatitis C virus (HCV) are intimately involved in orchestrating morphogenesis. Since it is dispensable for HCV RNA replication, the non-structural viral protein NS2 is suggested to play a central role in HCV particle assembly. However, despite genetic evidences, we have almost no understanding about NS2 protein-protein interactions and their role in the production of infectious particles. Here, we used co-immunoprecipitation and/or fluorescence resonance energy transfer with fluorescence lifetime imaging microscopy analyses to study the interactions between NS2 and the viroporin p7 and the HCV glycoprotein E2. In addition, we used alanine scanning insertion mutagenesis as well as other mutations in the context of an infectious virus to investigate the functional role of NS2 in HCV assembly. Finally, the subcellular localization of NS2 and several mutants was analyzed by confocal microscopy. Our data demonstrate molecular interactions between NS2 and p7 and E2. Furthermore, we show that, in the context of an infectious virus, NS2 accumulates over time in endoplasmic reticulum-derived dotted structures and colocalizes with both the envelope glycoproteins and components of the replication complex in close proximity to the HCV core protein and lipid droplets, a location that has been shown to be essential for virus assembly. We show that NS2 transmembrane region is crucial for both E2 interaction and subcellular localization. Moreover, specific mutations in core, envelope proteins, p7 and NS5A reported to abolish viral assembly changed the subcellular localization of NS2 protein. Together, these observations indicate that NS2 protein attracts the envelope proteins at the assembly site and it crosstalks with non-structural proteins for virus assembly

Imports and isotopes: a modern baseline study for interpreting Iron Age and Roman trade in fallow deer antlers

The European Fallow deer (Dama dama dama) became extinct in the British Isles and most of continental Europe at the time of the Last Glacial Maximum, with the species becoming restricted to an Anatolian refugium (Masseti et al. 2008). Human-mediated reintroductions resulted in fallow populations in Rhodes, Sicily, Mallorca, Iberia and other parts of western Europe (Sykes et al. 2013). Eventually, the species was brought to Britain by the Romans during the 1st century AD, with a breeding population being established at Fishbourne Roman Palace (Sykes et al. 2011). The human influence on the present-day distribution of the species makes it particularly interesting from a zooarchaeological perspective. This paper describes my MSc research, as part of the AHRC-funded project Dama International: Fallow Deer and European Society 6000 BC–AD 1600, looking at antlers from Iron Age and Roman sites in Britain for evidence of trade in body parts and whether this can be elucidated by a parallel stable isotope study of modern fallow antlers of known provenance

Sur un obturateur automatique

Bourget Henry, Carrère I. Sur un obturateur automatique. In: Bulletin astronomique, tome 22, 1905. pp. 76-79

Neonatal Mortality, Young Calf Slaughter and Milk Production during the Early Neolithic of North Western Mediterranean

TheNorth-WesternMediterraneanwitnessed a rapid expansion of farmers and their livestock during the EarlyNeolithic period. Depending on the region, cattle played a more or less important role in these communities; however how these animalswere exploited for theirmilk is not clear. Herewe investigate calfmortality to determine indirectly whether cattle dairying was practised by Early Neolithic stock herders. Age-at-death (AtD) frequencies for calves from two sites: Trasano (Italy, Impressa culture: 7–6th millennium BC) and La Draga (Spain, Cardial culture: 6th millennium BC) were estimated from dental eruption and development stages, and measurements of un-fused post-cranial material. Adult age classes are well represented in the dental AtD frequencies and were interpreted as the result of the slaughter of prime beef and retired lactating females. For calves aged less than 12 months, there was no statistical difference in the AtD frequencies based on dental and post-cranial material indicating that the data is a good representation of the mortality patterns of calves, either natural or deliberate. At both sites there was a strong mortality peak at 3–6months in all AtD profiles. At La Draga, this peak was clearly differentiated from a peak at 0–1month, which can be interpreted neonatal mortality possible a consequence of the birthing season coinciding with the end of winter during more humid climatic conditions that at present. The deliberate slaughter peak around 3–6months is discussed, and we propose that stock herders controlled the mortality of infant classes, possibly in response to variable external environment pressures while maintaining animal productivity