Engineering a serum-resistant and thermostable vesicular stomatitis virus G glycoprotein for pseudotyping retroviral and lentiviral vectors.

Vesicular stomatitis virus G glycoprotein (VSV-G) is the most widely used envelope protein for retroviral and lentiviral vector pseudotyping; however, serum inactivation of VSV-G pseudotyped vectors is a significant challenge for in vivo gene delivery. To address this problem, we conducted directed evolution of VSV-G to increase its resistance to human serum neutralization. After six selection cycles, numerous common mutations were present. On the basis of their location within VSV-G, we analyzed whether substitutions in several surface exposed residues could endow viral vectors with higher resistance to serum. S162T, T230N and T368A mutations enhanced serum resistance, and additionally K66T, T368A and E380K substitutions increased the thermostability of VSV-G pseudotyped retroviral vectors, an advantageous byproduct of the selection strategy. Analysis of a number of combined mutants revealed that VSV-G harboring T230N+T368A or K66T+S162T+T230N+T368A mutations exhibited both higher in vitro resistance to human serum and higher thermostability, as well as enhanced resistance to rabbit and mouse serum. Finally, lentiviral vectors pseudotyped with these variants were more resistant to human serum in a murine model. These serum-resistant and thermostable VSV-G variants may aid the application of retroviral and lentiviral vectors to gene therapy

Dominant mobility modulation by the electric field effect at the LaAlO_3 / SrTiO_3 interface

Caviglia et al. [Nature (London) 456, 624 (2008)] have found that the superconducting LaAlO_3 / SrTiO_3 interface can be gate modulated. A central issue is to determine the principal effect of the applied electric field. Using magnetotransport studies of a gated structure, we find that the mobility variation is almost five times as large as the sheet carrier density. Furthermore, superconductivity can be suppressed at both positive and negative gate bias. These results indicate that the relative disorder strength strongly increases across the superconductor-insulator transition.Comment: 4 pages, 4 figure

Anisotropic strains and magnetoresistance of La_{0.7}Ca_{0.3}MnO_{3}

Thin films of perovskite manganite La_{0.7}Ca_{0.3}MnO_{3} were grown epitaxially on SrTiO_3(100), MgO(100) and LaAlO_3(100) substrates by the pulsed laser deposition method. Microscopic structures of these thin film samples as well as a bulk sample were fully determined by x-ray diffraction measurements. The unit cells of the three films have different shapes, i.e., contracted tetragonal, cubic, and elongated tetragonal for SrTiO_3, MgO, and LaAlO_3 cases, respectively, while the unit cell of the bulk is cubic. It is found that the samples with cubic unit cell show smaller peak magnetoresistance than the noncubic ones do. The present result demonstrates that the magnetoresistance of La_{0.7}Ca_{0.3}MnO_{3} can be controlled by lattice distortion via externally imposed strains.Comment: Revtex, 10 pages, 2 figure

Electron-boson spectral density of LiFeAs obtained from optical data

We analyze existing optical data in the superconducting state of LiFeAs at $T =$ 4 K, to recover its electron-boson spectral density. A maximum entropy technique is employed to extract the spectral density $I^2\chi(\omega)$ from the optical scattering rate. Care is taken to properly account for elastic impurity scattering which can importantly affect the optics in an $s$-wave superconductor, but does not eliminate the boson structure. We find a robust peak in $I^2\chi(\omega)$ centered about $\Omega_R \cong$ 8.0 meV or 5.3 $k_B T_c$ (with $T_c =$ 17.6 K). Its position in energy agrees well with a similar structure seen in scanning tunneling spectroscopy (STS). There is also a peak in the inelastic neutron scattering (INS) data at this same energy. This peak is found to persist in the normal state at $T =$ 23 K. There is evidence that the superconducting gap is anisotropic as was also found in low temperature angular resolved photoemission (ARPES) data.Comment: 17 pages, 6 figure

Parity violation in deuteron photo-disintegration

We analyze the energy dependence for two types of parity-non-conserving (PNC) asymmetries in the reaction $\gamma D\to np$ in the near-threshold region. The first one is the asymmetry in reaction with circularly polarized photon beam and unpolarized deuteron target. The second one corresponds to those with an unpolarized photon beam and polarized target. We find that the two asymmetries have quite different energy dependence, and their shapes are sensitive to the PNC-meson exchange coupling constants. The predictions for the future possible experiments to provide definite constraints for the PNC-coupling constants are discussed.Comment: 22 pages, 12 figures. Submitted to Phys.Rev.C 10Oct.0

A review of near-wall Reynolds-stress

The advances made in second-order near-wall turbulence closures are summarized. All closures examined are based on some form of high Reynolds number models for the Reynolds stress and the turbulent kinetic energy dissipation rate equations. Consequently, most near-wall closures proposed to data attempt to modify the high Reynolds number models for the dissipation rate equation so that the resultant models are applicable all the way to the wall. The near-wall closures are examined for their asymptotic behavior so that they can be compared with the proper near-wall behavior of the exact equations. A comparison of the closure's performance in the calculation of a low Reynolds number plane channel flow is carried out. In addition, the closures are evaluated for their ability to predict the turbulence statistics and the limiting behavior of the structure parameters compared to direct simulation data

Studies of new media radiation induced laser

Various lasants were investigated especially, 2-iodohepafluoropropane (i-C3F7I) for the direct solar pumped lasers. Optical pumping of iodine laser was achieved using a small flashlamp. Using i-C3F7I as a laser gain medium, threshold inversion density, small signal gain, and laser performance at the elevated temperature were measured. The experimental results and analysis are presented. The iodine laser kinetics of the C3F7I and IBr system were numerically simulated. The concept of a direct solar-pumped laser amplifier using (i-C3F7I) as the laser material was evaluated and several kinetic coefficients for i-C3F7I laser system were reexamined. The results are discussed

Non-abelian dynamics in first-order cosmological phase transitions

Bubble collisions in cosmological phase transitions are explored, taking the non-abelian character of the gauge fields into account. Both the QCD and electroweak phase transitions are considered. Numerical solutions of the field equations in several limits are presented.Comment: 8 pages, 2 figures. Contribution to the CosPA 2003 Cosmology and Particle Astrophysics Symposium. Typos correcte

Role of Charged Gauge Fields in Generating Magnetic Seed Fields in Bubble Collisions during the Cosmological Electroweak Phase Transition

We calculate the magnetic field generated during bubble collisions in a first-order electroweak phase transition that may occur for some choices of parameters in the minimal supersymmetric Standard Model. We show that for sufficiently gentle collisions, where the Higgs field is relatively unperturbed in the bubble overlap region, the equations of motion can be linearized so that in the absence of fermions the charged W fields are the source of the electromagnetic current for generating the seed fields. Solutions of the equations of motion for the charged gauge fields and Maxwell's equations for the magnetic field in O(1,2) space-time symmetry are expressed in closed form by applying boundary conditions at the time of collision. Our results indicate that the magnetic fields generated by charged $W^{\pm}$ fields in the collision are comparable to those found in previous work. The magnetic fields so produced could seed galactic and extra-galactic magnetic fields observed today.Comment: 15 Pages, 7 Figure

Unified Analysis of Cosmological Perturbations in Generalized Gravity

In a class of generalized Einstein's gravity theories we derive the equations and general asymptotic solutions describing the evolution of the perturbed universe in unified forms. Our gravity theory considers general couplings between the scalar field and the scalar curvature in the Lagrangian, thus includes broad classes of generalized gravity theories resulting from recent attempts for the unification. We analyze both the scalar-type mode and the gravitational wave in analogous ways. For both modes the large scale evolutions are characterized by the same conserved quantities which are valid in the Einstein's gravity. This unified and simple treatment is possible due to our proper choice of the gauges, or equivalently gauge invariant combinations.Comment: 4 pages, revtex, no figure