Optimal SVM Switching for a Multilevel Multi-Phase Machine using Modified Discrete PSO

This paper searches for the best possible switching sequence in a multilevel multi-phase inverter that gives the lowest amount of voltage harmonics. A modified discrete particle swarm (MDPSO) algorithm is used in an attempt to find the optimal space vector modulation switching sequence that results in the lowest voltage THD. As with typical PSO cognitive and social parameters are used to guide the search, but an additional mutation term is added to broaden the amount of area searched. The search space is the feasible solutions for the predetermined vectors at a given modulation index. Comparison of the MDPSO algorithm to an integer particle swarm optimization (IPSO) is presented for all three modulation indices tested. The resulting switching sequences found show that the MDPSO algorithm is capable of finding a minimal THD solution for all modulations indices tested. The MDPSO algorithm performed better overall than the IPSO in terms of converging to the best solution with significantly lower iterations

Extending Voltage Range and Reducing Torque Ripple of Five-Phase Motor Drives with Added Voltage Harmonics

As multi-phase (defined as greater than three-phase) drives become more popular and practical, new research in this area investigates potential advantages including lower torque ripple and better power density. The added dimensions of a multi-phase machine leads to a completely different operating nature than standard three-phase machines. It can be shown physically and mathematically that certain harmonics do not contribute to torque production and therefore the torque is not directly tied to the current wave-shape. This paper utilizes this property to demonstrate a substantial increase in voltage range and a reduction in torque ripple through the use of added voltage harmonics. An analysis of a five-phase motor is presented followed by a range of modulation techniques. It is shown that by proper selection of third, fifth, and seventh harmonics, the required dc voltage can be reduced by eighteen percent and the torque ripple can be reduced by nearly sixty percent over traditional methods at the expense of higher current THD; which may not be a disadvantage in certain applications. Further investigation is then carried out in applying a unique space-vector modulation patter to the five-phase motor drive. This further reduces the torque ripple. Detailed simulation and laboratory tests are used to demonstrate this concept

Microsc Microanal

Abstract A microcompressor is a precision mechanical device that flattens and immobilizes living cells and small organisms for optical microscopy, allowing enhanced visualization of sub-cellular structures and organelles. We have developed an easily fabricated device, which can be equipped with microfluidics, permitting the addition of media or chemicals during observation. This device can be used on both upright and inverted microscopes. The apparatus permits micrometer precision flattening for nondestructive immobilization of specimens as small as a bacterium, while also accommodating larger specimens, such as Caenorhabditis elegans, for long-term observations. The compressor mount is removable and allows easy specimen addition and recovery for later observation. Several customized specimen beds can be incorporated into the base. To demonstrate the capabilities of the device, we have imaged numerous cellular events in several protozoan species, in yeast cells, and in Drosophila melanogaster embryos. We have been able to documen

Disorders of sex development : insights from targeted gene sequencing of a large international patient cohort

Background: Disorders of sex development (DSD) are congenital conditions in which chromosomal, gonadal, or phenotypic sex is atypical. Clinical management of DSD is often difficult and currently only 13% of patients receive an accurate clinical genetic diagnosis. To address this we have developed a massively parallel sequencing targeted DSD gene panel which allows us to sequence all 64 known diagnostic DSD genes and candidate genes simultaneously. Results: We analyzed DNA from the largest reported international cohort of patients with DSD (278 patients with 46, XY DSD and 48 with 46, XX DSD). Our targeted gene panel compares favorably with other sequencing platforms. We found a total of 28 diagnostic genes that are implicated in DSD, highlighting the genetic spectrum of this disorder. Sequencing revealed 93 previously unreported DSD gene variants. Overall, we identified a likely genetic diagnosis in 43% of patients with 46, XY DSD. In patients with 46, XY disorders of androgen synthesis and action the genetic diagnosis rate reached 60%. Surprisingly, little difference in diagnostic rate was observed between singletons and trios. In many cases our findings are informative as to the likely cause of the DSD, which will facilitate clinical management. Conclusions: Our massively parallel sequencing targeted DSD gene panel represents an economical means of improving the genetic diagnostic capability for patients affected by DSD. Implementation of this panel in a large cohort of patients has expanded our understanding of the underlying genetic etiology of DSD. The inclusion of research candidate genes also provides an invaluable resource for future identification of novel genes

Expression of insulin-like factor 3 protein in the rat testis during fetal and postnatal development and in relation to cryptorchidism induced by in utero exposure to Di (n-butyl) phthalate

Cryptorchidism is a common reproductive abnormality, possibly resulting from abnormal hormone production/action by the fetal testis. Insulin-like factor 3 (Insl3) is thought to be involved in gubernaculum development and transabdominal testicular descent, but its importance is unclear, due partly to lack of suitable Insl3 antibodies. We generated (by genetic immunization) and validated a novel antirat Insl3 antibody, which we used to characterize immunoexpression of Insl3 in rat Leydig cells (LCs) from fetal life until adulthood and its relationship to cryptorchidism. Immunoexpression was strong on embryonic day (E) 17.5 and E19.5 and from 35 d of age onward but weak from E21.5 until puberty. Because in utero exposure to di (n-butyl) phthalate (DBP) induces cryptorchidism and suppresses Insl3 gene expression, we investigated Insl3 protein expression in fetal and adult rats exposed to 500 mg/kg.d DBP from E13.5 to E21.5. Expression on E17.5 and E19.5 decreased dramatically after DBP exposure, but there was no consistent correlation between this suppression and abnormal testis position. We also compared expression of Insl3 and P450 side-chain cleavage enzyme in fetal testes from rats exposed in utero to DBP or flutamide (50 mg/kg.d). DBP treatment suppressed expression of both P450 side-chain cleavage enzyme and Insl3 at E19.5, but flutamide exposure had no effect on either protein, demonstrating that Insl3 expression in fetal rat LCs is not androgen regulated. In adult rats, Insl3 expression was suppressed in 80% of cryptorchid and 50% of scrotal testes from rats exposed to DBP, suggesting that prenatal DBP exposure also leads to maldevelopment/malfunction of the adult LC population in some animals.Chris McKinnell, Richard M. Sharpe, Kim Mahood, Nina Hallmark, Hayley Scott, Richard Ivell, Christophe Staub, Bernard Jégou, Friedrich Haag, Friedrich Koch-Nolte, and Stefan Hartun