Implicit association test as an analogical learning task

The Implicit Association Test (IAT) is a popular tool for measuring attitudes. We suggest that performing an IAT could, however, also change attitudes via analogical learning. For instance, when performing an IAT in which participants categorize (previously unknown) Chinese characters, flowers, positive words, and negative words, participants could infer that Chinese characters relate to flowers as negative words relate to positive words. This analogy would imply that Chinese characters are opposite to flowers in terms of valence and thus that they are negative. Results from three studies (N = 602) confirmed that evaluative learning can occur when completing an IAT, and suggest that this effect can be described as analogical. We discuss the implications of our findings for research on analogy and research on the IAT as a measure of attitudes

The 3D printing of a polymeric electrochemical cell body and its characterisation

An undivided flow cell was designed and constructed using additive manufacturing technology and its mass transport characteristics were evaluated using the reduction of ferricyanide, hexacyanoferrate (III) ions at a nickel surface. The dimensionless mass transfer correlation Sh = aRebScdLee was obtained using the convective-diffusion limiting current observed in linear sweep voltammetry; this correlation compared closely with that reported in the literature from traditionally machined plane parallel rectangular flow channel reactors. The ability of 3D printer technology, aided by computational graphics, to rapidly and conveniently design, manufacture and re-design the geometrical characteristics of the flow cell ishighlighted

The shared features principle : if two objects share a feature, people assume those objects also share other features

In this article we introduce the shared features principle, which refers to the idea that when 2 stimuli share 1 feature, people often assume that they share other features as well. This principle can be recognized in several known psychological phenomena, most of which were until now never considered to be related in this way. To illustrate the generative power of the principle, we report 8 preregistered studies (n = 1,614) in which participants completed an acquisition phase containing 3 stimuli: a neutral target, a positive source, and a negative source. Our results indicate that behavioral intentions, automatic evaluations, and self-reported ratings of a target object were influenced by the source object with which the target shared a feature. This was even the case when participants were told that there was no relation between source and target objects. Taken together, the shared features principle appears to be general, reliable, and replicable across a range of measures in the attitude domain. We close with a discussion of its theoretical implications, relevance to many areas of psychological science, as well as its heuristic and predictive value

Integrated management of childhood illness - A new approach to old diseases

No Abstrac

Evaluation of immune responses following infection of ponies with an EHV-1 ORF1/2 deletion mutant

Equine herpesvirus-1 (EHV-1) infection remains a significant problem despite the widespread use of vaccines. The inability to generate a protective immune response to EHV-1 vaccination or infection is thought to be due to immunomodulatory properties of the virus, and the ORF1 and ORF2 gene products have been hypothesized as potential candidates with immunoregulatory properties. A pony infection study was performed to define immune responses to EHV-1, and to determine if an EHV-1 ORF1/2 deletion mutant (ΔORF1/2) would have different disease and immunoregulatory effects compared to wild type EHV-1 (WT). Infection with either virus led to cytokine responses that coincided with the course of clinical disease, particularly the biphasic pyrexia, which correlates with respiratory disease and viremia, respectively. Similarly, both viruses caused suppression of proliferative T-cell responses on day 7 post infection (pi). The ΔORF1/ORF2 virus caused significantly shorter primary pyrexia and significantly reduced nasal shedding, and an attenuated decrease in PBMC IL-8 as well as increased Tbet responses compared to WT-infected ponies. In conclusion, our findings are (i) that infection of ponies with EHV-1 leads to modulation of immune responses, which are correlated with disease pathogenesis, and (ii) that the ORF1/2 genes are of importance for disease outcome and modulation of cytokine responses

Developmental Expression of Secretory β-1,4-endoglucanases in the Subventral Esophageal Glands of Heterodera glycines

Two β-1,4-endoglucanases (EGases), Hg-eng-1 and Hg-eng-2, were recently cloned from the soybean cyst nematode, Heterodera glycines, and their expression was shown in the subventral esophageal glands of hatched second-stage juveniles (J2). We examined the expression of these EGases in the subventral glands of all post-embryonic life stages of H. glycines by in situ hybridization and immunolocalization. The first detectable accumulation of EGase mRNAs occurred in the subventral glands of unhatched J2. EGase transcripts remained detectable in J2 after hatching and during subsequent root invasion. However, in late parasitic J2 and third-stage juveniles (J3), the percentage of individuals that showed EGase transcripts decreased. In female fourth-stage juveniles and adult females, EGase transcripts were no longer detected in the subventral glands. EGase hybridization signal reappeared in unhatched males coiled within the J3 cuticle, and transcripts were also present in the subventral glands of migratory adult males. Immunofluorescence labeling showed that EGase translation products are most abundantly present in the subventral glands of preparasitic J2, migratory parasitic J2, and adult males. The presence of EGases predominantly in the migratory stages suggests that the enzymes are used by the nematodes to soften the walls of root cells during penetration and intracellular migration

Signal Peptide-Selection of cDNA Cloned Directly from the Esophageal Gland Cells of the Soybean Cyst Nematode Heterodera glycines

Secretions from the esophageal gland cells of plantparasitic nematodes play critical roles in the nematodeparasitic cycle. A novel method to isolate cDNA encoding putative nematode secretory proteins was developed that utilizes mRNA for reverse transcription-polymerase chain reaction derived from microaspiration of the esophageal gland cell contents of parasitic stages of the soybean cyst nematode Heterodera glycines. The resulting H. glycines gland cell cDNA was cloned into the pRK18 vector, and plasmid DNA was transformed into a mutated yeast host for specific selection of cDNA inserts that encode proteins with functional signal peptides. Of the 223 cDNA clones recovered from selection in yeast, 97% of the clones encoded a predicted signal peptide. Fourteen unique cDNA clones hybridized to genomic DNA of H. glycines on Southern blots and, among them, nine cDNA clones encoded putative extracellular proteins, as predicted by PSORT II computer analysis. Four cDNA clones hybridized to transcripts within the dorsal esophageal gland cell of parasitic stages of H. glycines, and in situ hybridization within H. glycines was not detected for eight cDNA clones. The protocol provides a direct means to isolate potential plant-parasitic nematode esophageal gland secretory protein genes