The Features of Acute Brucellosis in Azerbaijan Republic

It is well known, that the injury of organs and systems and also immunological rates considerably determine clinical course and the result of brucellosis. In the article are presented the results of examination of 120 patients with acute brucellosis. The control group included 30 practically healthy persons. Groups were representative by the age and sex. The diagnosis of brucellosis was set on the base of complaints, anamnesis, epidemiological and clinical data and the results of serological examination of the blood. All patients also underwent the fixation of interleukin -6, іinterleukin-4 and TLR-2 in the blood serum. The detailed epidemiological, clinical and immunological characteristic of patients with brucellosis was presented. Among examined persons prevailed men, persons of young able-bodied persons and rural dwellers. It was revealed, that the dominating way of transmission was the contact one. The highest level of infection was fixed among the workers of farm enterprises and veterinarians. The season features were also revealed, the highest level of morbidity was in summer-autumn period. The main clinical features were: fever, arthralgia, risen disposition to perspire, hepatosplenomegaly. It was revealed, that the level of pro-inflammatory interleukins considerably prevailed over the level of anti-inflammatory ones. Despite the high sensitivity of PCR diagnostics, the positive results in our research were received only in one third of patients

Il-4 Polymorphism in Patients with Acute Brucellosis in the Republic of Azerbaijan

Currently, there are isolated data on the relationship between IL-4 polymorphism (C-589T) and susceptibility to brucellosis. Therefore, the purpose of the study was to determine the frequency of polymorphism of IL-4 (C-589T) in patients with acute brucellosis.Materials and methods: the article presents the results of surveys of 120 patients with acute brucellosis. The control group consisted of 30 practically healthy persons. The diagnosis of brucellosis was based on complaints, anamnesis, epidemiological and clinical data, and the results of a specific study. The definition of the polymorphism of IL-4 (C-589T) was performed for all patients in the main and control group. A detailed description of patients with brucellosis is presented. Among the observed prevailed men and young people of working age.Results: A mild degree was established in 74 (61.66 %) patients, while severe - only in 11 (9.17 %) patients. It was determined that the genotype CT (65.83 %) was significantly more common among patients with brucellosis than in the control group, where the frequency of this genotype was only 16.66 %. While the CC genotype was 3.2 times more common among healthy individuals than among patients in the main group (76.67 % versus 24.17 %), it was found that the CC genotype of the IL-4 gene was 13 times more common in patients for acute brucellosis with mild progress than with severe.Conclusions: The genotype IL-4 (C-589T) SS is significantly associated with the mild course of brucellosis

Analysis of antimutagenic effect of a compositional preparation made from extracts of green tea leaves and Caucasian persimmon fruits

Antimutagenic activity of a compositional preparation produced from extracts of green tea leaves and fruit of Caucasian persimmon was determined on the cell culture of human peripheral blood lymphocytes with modification of a mutation process induced by ionizing radiation and nitrosoguanidine. Antimutagenic efficiency of the preparation composites increases when the induced mutability is modified by factors of various physical and chemical nature. The comparative evaluation of their antimutagenic efficiency showed high antimutagenic activity of the composites of the preparation produced from extracts of green tea leaves and Caucasian persimmon fruit. Therewith the strength of this effect is dependent on dose intensity

Analysis of antimutagenic effect of a compositional preparation made from extracts of green tea leaves and Caucasian persimmon fruits

Antimutagenic activity of a compositional preparation produced from extracts of green tea leaves and fruit of Caucasian persimmon was determined on the cell culture of human peripheral blood lymphocytes with modification of a mutation process induced by ionizing radiation and nitrosoguanidine. Antimutagenic efficiency of the preparation composites increases when the induced mutability is modified by factors of various physical and chemical nature. The comparative evaluation of their antimutagenic efficiency showed high antimutagenic activity of the composites of the preparation produced from extracts of green tea leaves and Caucasian persimmon fruit. Therewith the strength of this effect is dependent on dose intensity

ДИНАМІКА БІОХІМІЧНИХ ПОКАЗНИКІВ, IL-4 ТА IL-6 ПРИ ГОСТРОМУ БРУЦЕЛЬОЗІ ПІД ВПЛИВОМ ЛІКУВАННЯ

Brucellosis remains a global problem for many countries around the world. The aim of our study was to determine the dynamics of serum levels of IL-4 and IL-6 in the patients with acute brucellosis before and after treatment. Materials and methods. We examined 178 patients with clinic signs of acute brucellosis who applied for medical assistance. Specific methods of the study were conducted by ELISA with the detection of IgM and IgG. From 178 surveyed patients, only 120 persons were included in the main group according to the criteria. The control group consisted of 30 practically healthy persons. Among the surveyed, men were in the majority – 75.0 %. The average age of the patients with brucellosis was (35.9±2.8) years. Results and discussion. Among the patients with acute brucellosis, the most commonly observed were such clinical symptoms: fever – 86.7 %, general weakness – 87.5 %, sweating in 70.0 % of patients, and arthralgia in 64.2 % of patients. At the same time, the least of all patients indicated the presence of myalgia – in 35.0 % of patients and weight loss – in 25.8 % of patients. In the objective survey, it was found that 77.5 % of patients had certain problems of the cardiovascular system, whereas only one patient (0.8 %) had disorders of the sexual system in the form of orchitis. A reliable difference was found between the overall blood count and the biochemical parameters among the main group before and after treatment and healthy individuals. It was found that the level of IL-4 in the serum of patients with acute brucellosis before treatment was (11.3±0.7) and the level of IL-6 was (15.3±1.4) pg/ml versus (15.3±1,4) and (8.5±0.9) pg/ml after therapy, respectively. Conclusions. The level of hemoglobin in patients with acute brucellosis was 1.2 times lower compared with the control group. The levels of ALT and AST before treatment were 1.7 and 1.9 times higher, respectively, compared to post-treatment and 1.9 and 2.5 times higher compared to control group. IL-4 and IL-6 levels were 1.6 and 1.8 times higher in patients with acute brucellosis before and after treatment, and 1.8 and 2.0 times higher than healthy subjects.Бруцельоз залишається глобальною проблемою для багатьох країн. Метою дослідження було визначення динаміки показників IL-4 та Il-6 у хворих на гострий бруцельоз під впливом лікування. Пацієнти і методи. Обстежено 178 хворих з клінікою гострого бруцельозу, які зверталися за медичною допомогою. Специфічні методи дослідження проводили методом ІФА з виявленням IgM та IgG. Згідно з критеріями включення в дослідження, зі 178 обстежених хворих повністю відповідало всім критеріям лише 120 осіб, які і склали основну групу. До конт­рольної групи увійшло 30 практично здорових осіб. Серед обстежених переважали особи чоловічої статі – 75,0 %. Середній вік пацієнтів з бруцельозом становив (35,9±2,8) року. Результати досліджень та їх обговорення. У хворих на гострий бруцельоз найчастіше виявляли наступні клінічні симптоми: гарячку – у 86,7 % осіб, загальну слабкість – у 87,5 %, пітливість – у 70,0 %, а також артралгії у 64,2 % пацієнтів. Тоді як найрідше хворі вказували на наявність міалгій – у 35,0 % та втрату маси тіла – у 25,8 % хворих. При об’єктивному обстежені було виявлено, що 77,5 % осіб мали ті чи інші проблеми з боку серцево-судинної системи, тоді як ураження статевої системи у вигляді орхіту мало місце лише в 1 хворого (0,8 %). Була встановлена достовірна різниця між показниками загального аналізу крові та біохімічних показників серед основної групи до та після лікування і здоровими особами. Так, виявлено, що рівень IL-4 в сироватці крові хворих на гострий бруцельоз до лікування становив (11,3±0,7), а рівень IL-6 – (15,3±1,4) пг/мл проти (15,3±1,4) та (8,5±0,9) пг/мл після терапії відповідно. Висновки. Рівні АЛТ та АСТ до лікування були в 1,7 та 1,9 разу вищими порівняно з показниками після терапії та в 1,9 і в 2,5 разу – порівняно з показниками контрольної групи. Рівні IL-4 та IL-6 були в 1,6 та в 1,8 разу вищими у хворих на гострий бруцельоз до лікування, та в 1,8 та в 2,0 рази вищими порівняно зі здоровими особами