Cereal density and N-fertiliser effects on the flora and biodiversity value of arable headlands

Modern intensive farming caused pronounced changes to the European arable flora. Many species adapted to less intensive traditional farming declined severely, as did the potential of unsown arable vegetation to support higher trophic levels. To reverse these trends, various agri-environment measures were introduced. One such measure is to manage cereal headlands as conservation headlands, involving strict restrictions on pesticide and fertiliser use. An additional modification to management which could reduce crop competition and thus deliver benefits to arable plants is cereal sowing at reduced rates. However, little is known about its benefits to rare and declining arable plants, or to species of value to higher trophic levels, and whether it can be implemented without concomitant increase in undesirable weeds. We set up identical two-factorial experiments in winter wheat and spring barley, combining a nitrogen fertiliser versus no fertiliser treatment with cereal sowing at economic rates versus sowing at rates reduced by 75 %, with added sowing of a mixture of rare arable species. Both experiments also included an uncropped but cultivated control equivalent to another agri-environment measure. Our results show that reduced cereal sowing in conservation headlands can benefit rare and declining species, as well as arable plant diversity, without necessarily resulting in a concomitant increase in undesirable weeds. While such benefits tended to be larger in uncropped cultivated controls, conservation headlands have the advantage of not requiring land being taken out of production. Moreover, as shown in this study, their benefits to arable plants can be maximised by reduced sowing

Discovery of Fragment Molecules That Bind the Human Peroxiredoxin 5 Active Site

The search for protein ligands is a crucial step in the inhibitor design process. Fragment screening represents an interesting method to rapidly find lead molecules, as it enables the exploration of a larger portion of the chemical space with a smaller number of compounds as compared to screening based on drug-sized molecules. Moreover, fragment screening usually leads to hit molecules that form few but optimal interactions with the target, thus displaying high ligand efficiencies. Here we report the screening of a homemade library composed of 200 highly diverse fragments against the human Peroxiredoxin 5 protein. Peroxiredoxins compose a family of peroxidases that share the ability to reduce peroxides through a conserved cysteine. The three-dimensional structures of these enzymes ubiquitously found throughout evolution have been extensively studied, however, their biological functions are still not well understood and to date few inhibitors have been discovered against these enzymes. Six fragments from the library were shown to bind to the Peroxiredoxin 5 active site and ligand-induced chemical shift changes were used to drive the docking of these small molecules into the protein structure. The orientation of the fragments in the binding pocket was confirmed by the study of fragment homologues, highlighting the role of hydroxyl functions that hang the ligands to the Peroxiredoxin 5 protein. Among the hit fragments, the small catechol molecule was shown to significantly inhibit Peroxiredoxin 5 activity in a thioredoxin peroxidase assay. This study reports novel data about the ligand-Peroxiredoxin interactions that will help considerably the development of potential Peroxiredoxin inhibitors

Bumblebee family lineage survival is enhanced in high quality landscapes

Insect pollinators such as bumblebees (Bombus spp.) are in global decline1,2, a major cause of which is habitat loss due to agricultural intensification3. A range of global and national initiatives aimed at restoring pollinator habitats and populations have been developed4-6. However, the success of these initiatives depends critically upon understanding how landscape change affects key population-level parameters, such as survival between lifecycle stages7, in target species. Such understanding is lacking for bumblebees because of the difficulty of systematically finding and monitoring colonies in the wild. We used a novel combination of habitat manipulation, land-use and habitat surveys, molecular genetics8 and demographic and spatial modelling to examine between-year survival of family lineages in field populations of three bumblebee species. Here we show that the survival of family lineages from the summer worker to the spring queen stage in the following year increases significantly with the proportion of high-value foraging habitat, including spring floral resources, within 250-1000 m of the natal colony. This is the first evidence of a positive impact of habitat quality on survival and persistence between successive colony cycle stages in bumblebee populations. The findings provide strong support for conservation interventions that increase floral resources at a landscape scale and throughout the season having positive effects on wild pollinators in agricultural landscapes

Practical methods for the control of tor-grass (Brachypodium pinnatum s.l.) and the restoration of calcareous grassland

Calcareous grasslands are sites of high conservation value across Western Europe; however, they are increasingly threatened by the dominance of a native competitive grass, Brachypodium pinnatum, which reduces the diversity of the grassland. Despite this, there is no clear consensus on the most effective method for controlling B. pinnatum and restoring the grassland community. We established two experiments at a calcareous grassland of high nature conservation value in the UK, i) a herbicide spraying experiment with seeding and ii) a seasonal cut-and-graze experiment, to investigate the potential for reducing dense B. pinnatum cover and preventing further expansion of sparse cover, respectively. We examined the effect of different herbicide and cut-and-graze treatments on B. pinnatum cover, and on the species richness and diversity of the grassland over three consecutive years. Herbicide spraying reduced the cover of B. pinnatum, though two spray applications led to a greater reduction longer-term. Species richness and diversity initially declined with the herbicide spray, however this recovered rapidly to levels higher than before spraying commenced. Seeding the spray plots was beneficial for the establishment of Bromopsis erecta and potentially reduced the likelihood of re-colonisation by B. pinnatum and undesirable arable species. The cut-and-graze experiment also showed promising potential in terms of controlling the spread of B. pinnatum. Compared with a single cut in the spring or autumn, cutting and grazing twice, in both spring and autumn was found to reduce the cover of B. pinnatum, whilst also increasing species richness and diversity. Further monitoring is needed to determine the long-term effectiveness of this management treatment

Compendium of specialist alcohol support services for people from minority ethnic and migrant communities

This compendium was produced with funding from Manchester Metropolitan University and in collaboration with colleagues in the Culturally Appropriate Alcohol Services group, formed by the Midlands-based government department, the Office of Health Improvement and Disparities (OHID). It was compiled between September 2022 and March 2023. At the time of final compilation, a number of agencies had not responded to our request for information. While the initial impetus for the compendium resulted from an alcohol-focussed forum, this compendium also includes services that support people with other drug use

The potential to increase grassland soil C stocks by extending reseeding intervals is dependent on soil texture and depth

Grasslands account for ∼30% of global terrestrial carbon (C), of which most is stored in soils and provide important ecosystem services including livestock and forage production. Reseeding of temporary grasslands on a 5-year cycle is a common management practice to rejuvenate sward productivity and reduce soil compaction, but is physically disruptive and may reduce soil organic carbon (SOC) stocks. However, research to date is limited, which impacts on the ability to optimise grassland management for climate change mitigation. To determine whether extending the time interval up to 20 years between grassland reseeding can increase stable SOC stocks, a soil survey was conducted across three UK grassland chrono-sequences comprising 24 fields on contrasting soil types. We found that grassland SOC stocks (39.8–114.8 Mg C ha−1) were higher than co-located fields in arable rotations (29.3–83.2 Mg C ha−1) and the relationship with grassland age followed a curvilinear relationship with rapid SOC stock accumulation in the year following reseeding (2.69–18.3 Mg C ha−1 yr−1) followed by progressively slower SOC accumulation up to 20 years. Contrary to expectation, all grasslands had similar soil bulk densities and sward composition questioning the need for traditional 5-year reseeding cycles. Fractionation of soils into stable mineral associated fractions revealed that coarse textured grassland topsoils (0–15 cm) were near-saturated in C irrespective of grassland age whilst loam soils reached saturation ∼10 years after reseeding. Fine-textured topsoils and subsoils (15–30 cm) of all textures were under saturated and thus appear to hold the most potential to accrue additional stable C. However, the lack of a relationship between C saturation deficit and grassland age in subsoils suggests that more innovative management to promote SOC redistribution to depth, such as a switch to diverse leys or full inversion tillage may be required to maximise subsoil SOC stocks. Taken together our findings suggest that extending the time between grassland reseeding could temporarily increase SOC stocks without compromising sward composition or soil structure. However, detailed monitoring of the trade-offs with grassland productivity are required. Fine textured soils and subsoils (15–30 cm) have the greatest potential to accrue additional stable C due to under saturation of fine mineral pools

Long-term effects of hedgerow management policies on resource provision for wildlife

Hedgerows provide important habitat and food resources for overwintering birds, mammals and invertebrates. Currently, 41% of managed hedgerow length in England forms part of three Agri-Environment Scheme (AES) options, which specify a reduction in hedgerow cutting frequency from the most common practice of annual cutting. These AES options aim to increase the availability of flowers and berries for wildlife, but there has been little rigorous testing of their efficacy or estimates of the magnitude of their effects. We conducted a factorial experiment on hawthorn hedges to test the effects of (i) cutting frequency (every 1, 2 or 3 years) and (ii) timing of cutting (autumn vs. winter) on the abundance of flowers and berry resources. Results from 5 years show that hedgerow cutting reduced the number of flowers by up to 75% and the biomass of berries available over winter by up to 83% compared to monitored uncut hedges. Reducing cutting frequency from every year to every 3 years resulted in 2.1 times more flowers and a 3.4 times greater berry mass over 5 years. Cutting every 2 years had an intermediate effect on flower and berry abundance, but the increase in biomass of berries depended on cutting in winter rather than autumn. The most popular AES option is cutting every 2 years (32% of English managed hedgerow length). If these hedges were managed under a 3 year cutting regime instead, we estimate that biomass of berries would increase by about 40%, resulting in a substantial benefit for wildlife

Establishment and management of wildflower areas for insect pollinators in commercial orchards

Sown wildflower areas are increasingly recommended as an agri-environmental intervention measure, but evidence for their success is limited to particular insect groups or hampered by the challenges of establishing seed mixes and maintaining flower abundance over time. We conducted a replicated experiment to establish wildflower areas to support insect pollinators in apple orchards. Over three years, and across 23 commercial UK orchards with and without sown wildflowers, we conducted 828 transect surveys across various non-crop habitats. We found that the abundance of flower-visiting solitary bees, bumblebees, honeybees, and beetles was increased in sown wildflower areas, compared with existing non-crop habitats in control orchards, from the second year following floral establishment. Abundance of hoverflies and other non-syrphid flies was increased in wildflower areas from the first year. Beyond the effect of wildflower areas, solitary bee abundance was also positively related to levels of floral cover in other local habitats within orchards, but neither local nor wider landscape-scale context affected abundance of other studied insect taxa within study orchards. There was a change in plant community composition on the sown wildflower areas between years, and in patterns of flowering within and between years, showing a succession from unsown weedy species towards a dominance of sown species over time. We discuss how the successful establishment of sown wildflower areas and delivery of benefits for different insect taxa relies on appropriate and reactive management practices as a key component of any such agri-environment scheme

Population responses of honeybees to oilseed rape neonicotinoid seed treatments in Hungary, Germany and the UK

The data set describes the effects of three neonicotinoid seed treatments (clothianidin, thiamethoxam and a control) applied to winter sown oilseed rape in Hungary, Germany and the UK on honeybees (Apis mellifera). The data describes population responses in terms of European Food Safety Authority (EFSA) primary (colony strength and overwintering success) and secondary assessment endpoints for the response of honeybees to exposure to the neonicotinoids. Information on expression of neonicotinoids in the pollen and nectar from the crop or collected by bees is also included, as well as details of honeybee diseases and foraging preferences