Metal Core Bonding Motifs of Monodisperse Icosahedral Au13 and Larger Au Monolayer-Protected Clusters As Revealed by X-ray Absorption Spectroscopy and Transmission Electron Microscopy

The atomic metal core structures of the subnanometer clusters Au13[PPh3]4[S(CH2)11CH3]2Cl2 (1) and Au13[PPh3]4[S(CH2)11CH3]4 (2) were characterized using advanced methods of electron microscopy and X-ray absorption spectroscopy. The number of gold atoms in the cores of these two clusters was determined quantitatively using high-angle annular dark field scanning transmission electron microscopy. Multiple-scattering-path analyses of extended X-ray absorption fine structure (EXAFS) spectra suggest that the Au metal cores of each of these complexes adopt an icosahedral structure with a relaxation of the icosahedral strain. Data from microscopy and spectroscopy studies extended to larger thiolate-protected gold clusters showing a broader distribution in nanoparticle core sizes (183 ± 116 Au atoms) reveal a bulklike fcc structure. These results further support a model for the monolayer-protected clusters (MPCs) in which the thiolate ligands bond preferentially at 3-fold atomic sites on the nanoparticle surface, establishing an average composition for the MPC of Au180[S(CH2)11CH3]40. Results from EXAFS measurements of a gold(I) dodecanethiolate polymer are presented that offer an alternative explanation for observations in previous reports that were interpreted as indicating Au MPC structures consisting of a Au core, Au2S shell, and thiolate monolayer

Differentiation and Loss of Malignant Character of Spontaneous Pulmonary Metastases in Patient-Derived Breast Cancer Models

Patient-derived human-in-mouse xenograft models of breast cancer (PDX models) that exhibit spontaneous lung metastases offer a potentially powerful model of cancer metastasis. In this study, we evaluated the malignant character of lung micro-metastases that emerge in such models after orthotopic implantation of human breast tumor cells into the mouse mammary fat pad. Interestingly, relative to the parental primary breast tumors, the lung metastasis (met)-derived mammary tumors exhibited a slower growth rate and a reduced metastatic potential with a more differentiated epithelial status. Epigenetic correlates were determined by gene array analyses. Lung met-derived tumors displayed differential expression of negative regulators of cell proliferation and metabolism and positive regulators of mammary epithelial differentiation. Clinically, this signature correlated with breast tumor subtypes. We identified microRNA-138 as a novel regulator of invasion and epithelial-mesenchymal transition in breast cancer cells, acting by directly targeting the polycomb epigenetic regulator EZH2. Mechanistic investigations showed that GATA3 transcriptionally controlled miR-138 levels in lung metastases. Notably, the miR-138 activity signature served as a novel independent prognostic marker for patient survival beyond traditional pathologic variables, intrinsic subtypes or a proliferation gene signature. Our results highlight the loss of malignant character in some lung micro-metastatic lesions and the epigenetic regulation of this phenotype

Real-time communication between virtual environments over the Internet

EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Qualitative Dynamics of Suspended Particulate Matter in the Changjiang Estuary from Geostationary Ocean Color Images: An Empirical, Regional Modeling Approach

The suspended particulate matter (SPM) in Changjiang Estuary is characterized by a high concentration of significant diurnal dynamics. With a higher temporal resolution (eight images obtained per day), Geostationary Ocean Color Imager (GOCI) was selected as the primary remote sensor for the dynamics monitoring in this paper, instead of other satellite sensor working in polar orbit. Based on the characteristics of the field spectra measured in the estuary, an empirical model was established with the band ratio of Rrs745 divided by Rrs490 and proven effective in Suspended Particulate Matter (SPM) estimation (R2 = 0.9376, RMSE = 89.32 mg/L). While, Validation results showed that the model performed better in coastal turbid waters than offshore clear waters with higher chlorophyll-a concentration, stressing the importance of partitioning SPM into its major components and doing separate analysis. The hourly observations from GOCI showed that the diurnal variation magnitudes exhibited clear regional characteristics, with a maximum in the turbidity belt near the mouth and a minimum in the offshore deeper areas. In addition, comparing the monthly averaged SPM distribution with the amount of sediment discharged into the estuary, the variation in estuarine turbidity maximum zone is more likely contributed by the sediments resuspended from the sea bed that has already accumulated in the estuarine delta

Implementing Manufacturing Message Specifications (MMS) within collaborative virtual environments over the Internet

Virtual reality (VR) is an emerging technology that has many potential applications in manufacturing. Networked or shared virtual environments (VE) are of growing interest in modern manufacturing, which is making increasing use of virtual reality technology. An important feature of networked VEs is the way in which they control and share 3D information, and the support of this by real-time data transfer. The bottleneck of transferring 3D data in real-time between networked VEs is the performance of networking protocols. This paper proposes a novel approach to implementing the Manufacturing Message Specifications (MMS), an ISO/IEC 9506 communication standard and presents a Manufacturing Message Transfer Protocol (MMTP), suitable for a broad range of Internet-based VE communication applications - in particular, virtual manufacturing collaborative design and control. An Internet communication system is described that can support networked virtual manufacturing environments within the framework of the Manufacturing Message Specifications (MMS) Standard

Stanniocalcin-1 Protects a Mouse Model from Renal Ischemia-Reperfusion Injury by Affecting ROS-Mediated Multiple Signaling Pathways

Stanniocalcin-1 (STC-1) protects against renal ischemia-reperfusion injury (RIRI). However, the molecular mechanisms remain widely unknown. STC-1 inhibits reactive oxygen species (ROS), whereas most ROS-mediated pathways are associated with ischemic injury. Therefore, to explore the mechanism, the effects of STC-1 on ROS-medicated pathways were studied. Non-traumatic vascular clamps were used to establish RIRI mouse models. The serum levels of STC-1, interleukin-6 (IL-6), interferon (IFN) γ, P53, and capase-3 were measured by ELISA kits. Superoxide dismutase (SOD) and malondialdehyde (MDA) were measured by fluorescence spectrofluorometer. All these molecules changed significantly in a RIRI model mouse when compared with those in a sham control. Kidney cells were isolated from sham and model mice. STC-1 was overexpressed or knockout in these kidney cells. The molecules in ROS-medicated pathways were measured by real-time quantitative PCR and Western blot. The results showed that STC-1 is an effective ROS scavenger. The serum levels of STC-1, MDA and SOD activity were increased while the serum levels of IL-6, iIFN-γ, P53, and capase-3 were decreased in a model group when compared with a sham control (p < 0.05). Furthermore, the levels of STC-1,p53, phosphorylated mitogen-activated protein kinase kinase (p-MEKK-1), c-Jun N-terminal kinase (p-JNK), extracellular signal-regulated kinase (p-ERK), IkB kinase (p-IKK), nuclear factor (NF) κB, apoptosis signal-regulating kinase 1 (ASK-1) and caspase-3 changed significantly in kidney cells isolated from a RIRI model when compared to those isolated from a sham control (p < 0.05). Meanwhile, STC-1 overexpression or silence caused significant changes of the levels of these ROS-mediated molecules. Therefore, STC-1 maybe improve anti-inflammation, anti-oxidant and anti-apoptosis activities by affecting ROS-mediated pathways, especially the phospho-modifications of the respective proteins, resulting in the increase of SOD and reduce of capase-3, p53, IL-6 and IFN-γ

Efficient Extraction and Isolation of Mangiferin from Mango Leaves by Ethyl Acetate Impurity Removal Method

[Objectives] To optimize the ethyl acetate impurity removal method for extracting and isolating mangiferin from mango leaves, and provide raw materials and technical support for development and use of mangiferin related products. [Methods] Five steps (material crushing → ethyl acetate impurity removing → concentrated extract washing → extracting with methanol → crystallization and precipitation) were used. The single factor experiment and L9(34) orthogonal experiment was carried out to optimize the process parameters including extraction time, ultrasonic power, extraction times, and extraction temperature. [Results] The optimum process of ethyl acetate impurity removal method for extracting and isolating mangiferin from mango leaves was as follows: the mango leaves were crushed and sieved; 3 mL/g of ethyl acetate was added, sealed and soaked for 4 h, ultrasonically shaken for 20 min (50℃, 350 W), filtered at room temperature, filtered with 100 mesh sieve, and extracted three times; added 100% methanol to the residue at 3 mL/g, extract by ultrasonic vibration for 20 min (350 W, 55℃) for four times, filtered with 100 mesh sieve when it was still hot; mixed the extract of each time, condensed by vacuum decompression to get the extract; added 100% methanol at 4 mL/g, mixed and washed for 5 min at room temperature, placed for 10 min, filtered with 100 mesh sieve, washed 3 times repeatedly, and dried the filter residue at 60℃ to obtain the crude mangiferin; added 100% methanol at 4 mL/g, mixed and washed at 50℃ for 5 min, placed at 6℃ for 8 h, dried the filter residue at 60℃, and repeatedly crystallized two times. According to the above process, crude and pure mangiferin products could be obtained, the purity of mangiferin of the crude product was higher than 64.00%, the total recovery rate was 83.90%, and the purity of mangiferin of the pure product was higher than 98.00%, and the total recovery rate was about 66.40%. [Conclusions] The optimized ethyl acetate impurity removal method is easy in operation, low in cost, and high in efficiency for extracting and isolating mangiferin, and can be applied for actual production of mangiferin

Visualisation in architecture, engineering and construction (AEC)

In the architecture, engineering and construction industries, computer visualization usage can cover the whole lifecycle of a product from presentation of initial concepts to the final stages of production and can also extend to maintenance issues. Three-dimensional walkthroughs can be created from hand drawn sketches at the very early stages of the design process. Three-dimensional models can be used by design teams to communicate design intent to client and users and to compare and evaluate design options. During more advanced stages of design, three-dimensional representations can be used to check the integrity of services coordination, accessibility and maintainability. During construction, visualization can facilitate the interpretation of design details by site operatives. The concept of visualization is not limited to modeling physical objects but can extend to the representation of abstract data sets of the type obtained from simulation programs used in performance assessment or from Computation Fluid Dynamics (CFD) applications. This paper will review the application of visualization in the process of design and construction and then present findings from three research projects that made use of some of these techniques at various stages of the process: for collaborative working during concept design stage, for design development and marketing in the house building sector, and for the modeling of design details during the construction stage

Variation of Suspended Particles in the Bottom Layer of the East China Sea with Data from Seafloor Observatory

The in situ scattering and transmissometry laser (LISST-100X), equipped with an acoustic wave and current (AWAC) meter and conductivity, temperature, and depth (CTD) instruments over the seabed in the East China Sea, was used to monitor the variation in suspended particles in the bottom sea layer, including particle size distribution (PSD) and volume concentration. The power law approximation was tested to describe the variability in PSD based on the field data. The results show that the power law was robust in processing continuous data, accompanied with the same optimal reference particle size (~63 μm) and little change in the corresponding exponent (~3.4) in both periods. Suspended particles were divided into three types: macroflocs (>133 μm), microflocs (36–133 μm), and single grains (<36 μm). Particle sizes were coarse during the two seasons, with macroflocs representing more than 60% of all the suspended particles, especially in February, when the particle size spectra were usually open-ended. Results from the harmonic analysis method indicate that tidal-induced resuspension and advection are the major reasons for the diurnal dynamics of sediments. Due to the tidal asymmetry in the region, we only found one mode in volume concentration at the moment of maximum velocity. However, the ratios of macroflocs were bimodal, with maximum floods and ebbs in one tidal cycle in February, when the higher mode at the maximum ebbs may be contributed to by the flocculation of finer particles considering the decreasing ratios of finer particles. Due to the enhanced stratification and the clean barrier built up by the Taiwan Warm Current in the southeast corner, the significant daily variation in suspended particles observed in February weakened in September. The influence of waves was uncertain, although the correlation coefficient between significant wave height and volume concentration was about 64% in February