Immunohistochemical detection of macrophage migration inhibitory factor in fetal and adult bovine epididymis: Release by the apocrine secretion mode?

Originally defined as a lymphokine inhibiting the random migration of macrophages, the macrophage migration inhibitory factor (MIF) is an important mediator of the host response to infection. Beyond its function as a classical cytokine, MIF is currently portrayed as a multifunctional protein with growth-regulating properties present in organ systems beyond immune cells. In previous studies, we detected substantial amounts of MIF in the rat epididymis and epididymal spermatozoa, where it appears to play a role during post-testicular sperm maturation and the acquisition of fertilization ability. To explore its presence in other species not yet examined in this respect, we extended the range of studies to the bull. Using a polyclonal antibody raised against MIF purified from bovine eye lenses, we detected MIF in the epithelium of the adult bovine epididymis with the basal cells representing a prominently stained cell type. A distinct accumulation of MIF at the apical cell pole of the epithelial cells and in membranous vesicles localized in the lumen of the epididynnal duct was obvious. In the fetal bovine epididymis, we also detected MIF in the epithelium, whereas MIF accumulation was evident at the apical cell surface and in apical protrusions. By immuno-electron microscopy of the adult bovine epididymis, we localized MIF in apical protrusions of the epithelial cells and in luminal membrane-bound vesicles that were found in close proximity to sperm cells. Although the precise origin of the MIF-containing vesicles remains to be delineated, our morphological observations support the hypothesis that they become detached from the apical surface of the epididymal epithelial cells. Additionally, an association of MIF with the outer dense fibers of luminal spermatozoa was demonstrated. Data obtained in this study suggest MIF release by an apocrine secretion mode in the bovine epididymis. Furthermore, MIF localized in the basal cells of the epithelium and in the connective tissue could be responsible for regulating the migration of macrophages in order to avoid contact of immune cells with spermatozoa that carry a wide range of potent antigens. Copyright (c) 2006 S. Karger AG, Basel

Worshipers and Warriors

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/68415/2/10.1177_009770047600200102.pd

'My language, my people': language and ethnic identity among British‐born South Asians

This study explores how a group of second generation Asians (SGA) understood and defined language, focusing upon the role they perceived language to have played in their identity. Twelve SGA were interviewed and the data were subjected to qualitative thematic analysis. Four superordinate themes are reported, entitled 'Mother tongue and self', #A sense of ownership and affiliation', 'Negotiating linguistic identities in social space' and 'The quest for a positive linguistic identity'. Participants generally expressed a desire to maintain continuity of self‐definition as Asian, primarily through the maintenance of the heritage language (HL). An imperfect knowledge of the HL was said to have a negative impact upon psychological well‐being. There were ambivalent responses to the perception of language norms, and various strategies were reported for dealing with dilemmatic situations and identity threat arising from bilingualism. Recommendations are offered for interventions that might aid the ‘management’ of bilingualism among SGA

PROFIL WISATAWAN MUSEUM RADYA PUSTAKA SURAKARTA

Anggit Margaret, C9407031 2011. Profil Wisatawan Museum Radya Pustaka Surakarta. Program Studi Diploma III Usaha Perjalanan Wisata Fakultas Sastra Dan Seni Rupa Universitas Sebelas Maret Surakarta. Penelitian tugas akhir ini mengkaji tentang Profil Wisatawan di Museum Radya Pustaka Surakarta. Tujuan dari penelitian ini adalah untuk mengetahui dari daerah mana saja wisatawan yang berkunjung ke Museum Radya Pustaka, bagaimana ciri-ciri wisatawan yang berkunjung ke Museum Radya Pustaka serta harapan-harapan yang diinginkan wisatawan terhadap Museum Radya Pustaka. Penelitian dilakukan dengan metode kualitatif. Pengumpulan data dilakukan melalui wawancara dengan narasumber wisatawan yang berkujung di Museum Radya Pustaka Surakarta tempat penulis melakukan penelitian, serta studi pustaka dan studi dokumen guna menambah sumber data. Hasil penelitian menunjukkan bahwa (1) Sebagian besar wisatawan yang datang berasal dari Semarang sebesar 32%. (2) Mayoritas wisatawan yang berkunjung ke Museum Radya Pustaka berusia antara 17-25 tahun dan kebanyakan dari mereka adalah pelajar atau mahasiswa dengan prosentase 52%. (3) Sebagian besar wisatawan yang datang ke Museum Radya Pustaka adalah bertujuan untuk melakukan penelitian yaitu sebesar 34%. (4) Harapan wisatawan yang berkunjung terhadap kelangsungan Museum Radya Pustaka sebagian besar adalah agar ditingkatkan lagi pengelolaan dan keamanan museum, agar kejadian hilangnya benda-benda koleksi museum tidak terulang lagi dikemudian hari. Kesimpulan dari hasil penelitian ini bahwa wisatawan yang berkujung ke Museum Radya Pustaka Surakarta mayoritas berasal dari Semarang, mayoritas berusia 17-25 tahun dan kebanyakan dari mereka adalah berprofesi sebagai pelajar dan mahasiswa. Kebanyakan wisatawan yang datang bertujuan untuk melakukan penelitian, serta harapan wisatawan terhadap Museum Radya Pustaka adalah supaya lebih ditingkatkan lagi pengelolaan dan keamanan museum

Laboratory investigation of rubble-mound breakwaters

Paper describing the development of the "Hudson formula" for the stability of armor on a breakwater

Laboratory investigations of rubble-mound breakwaters

This paper reports on a laboratory investigation conducted at the United States Army Engineer Waterways experiment station, Vickburg, Mississippi, to determine criteria for the design and construction of rubble mound breakwaters. Small-scale breakwater sections are hand-constructed in a concrete wave flume 119 ft long, 5ft wide, 4 ft deep, and subjected to mechanically generated waves to determine the stability of the armor units. A general stability equation has been derived and is being used to guide the experimental program and correlate the test data. From the test data obtained important unknown functions in the general stability equation have been determined for selected breakwater and test-wave conditions, and a new breakwater stability formula has been obtained. In conjunction with the stability tests, wave run-up data are obtained for each breakwater section and wave condition tested. Also, measurements are obtained that enable the thickness and porosity of cover layers composed of different types of armor units to be determined. The new stability formula and the experimental data obtained so far have provided essential information for an improved method of designing rubble-mound breakwaters with protective cover layers composed of quarry-stone and tetrapod armor units. Tests in progress (1959) to obtain experimental data for other special shapes of cast concrete armor units (cubes, tetrahedrons, and tribars) should increase considerably the accuracy of rubble-mound breakwater design