“Quiet & clever together”: reassessing the significance of elite women and the literary culture of the country house

This article examines elite women’s agency and participation in the literary life of the country house, focusing on the circle that centred on Jemima Marchioness Grey and her husband, Philip Yorke, at Wrest Park in Bedfordshire. The “Society at Wrest” was an exclusive group of close friends and family that included Grey’s childhood friends, Mary Gregory (née Grey) and Catherine Talbot, as well as her sisters-in-law, Elizabeth Anson and Margaret Heathcote (née Yorke). Most members of the Wrest Circle were careful about publishing their works, yet they found a cerebral escape in the shades of “Vacuna” – the name they affectionately gave to Wrest – as they created private literary compositions. Although Grey did not contribute to her friends’ literary compositions, she did, as hostess, play an invaluable role in providing a space at Wrest Park that facilitated and nurtured their intellectual and artistic endeavours. Disrupting the scholarly emphasis on public-facing female intellectuals, this article argues that for some elite women, the value of belonging to a coterie was not about attracting literary fame but rather having access to a permissive environment in which they could embark on private literary pursuits and belong to an exclusive and supportive intellectual network

Sub-State Nationalism and Intergovernmental Fiscal Policy: An Examination of the Netherlands and Spain

Nations without the distinct legal status of statehood have become increasingly important in the domestic politics of some European countries. Recent secession referenda in various countries (most notably Spain and the United Kingdom) have highlighted this trend. This study examines the impact that sub-state national identities have on the disbursement of intergovernmental transfer payments in two unitary states: the Netherlands and Spain. These cases were selected to conduct a “most different systems” analysis and examined transfers from the central governments to their respective highest levels of regional governance in the period between 2010 and 2018. Established theory on the interaction between identity and institutions supports the hypothesis that regions with more distinct separate identities will receive preferential treatment from their state governments than those without. This study used support for sub-state nationalist parties as a proxy measure for relative strength of their respective identities, which combined with the independent fiscal capacity of the regions was regressed against the amount of transfers received to understand any relationship that might exist. The results of the analysis of these variables indicates that although they are more effective predictors in Spain, there is a significant positive relationship in both countries between sub-state national identities and intergovernmental transfer payments. Individual analysis of the Netherlands found that, counter to established theory on intergovernmental transfers, regions with higher fiscal capacities also received more transfers from the government. Analysis of Spain found that, with a couple of notable exceptions, regions with higher degrees of sub-state nationalism received more transfers from the central government. This study fills gaps in the literature that previously did not specifically examine the relationships between these variables and highlights some areas for future study, both at smaller case levels and broader scales

CAP1 expression is developmentally regulated in Xenopus

We have cloned and characterized a Xenopus member of the cyclase associated protein (CAP) gene family. xCAP1 is expressed as a maternal transcript, but is up-regulated prior to gastrulation and subsequently localizes to head mesenchyme, lens, otic vesicle, and trunk mesoderm including the pronephros. At different stages, the gone also appears to differentiate surface from deep (sensorial) ectoderm. As in Drosophila, Xenopus CAP1 is expressed in the developing eye, specifically in the differentiating lens. However, in distinction to Drosophila, Xenopus CAP1 does not express in periodically arrayed neural bands. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved

Skh1, the MEK component of the mkh1 signaling pathway in Schizosaccharomyces pombe

Skip to Next Section We previously reported the identification of Mkh1, a MEK kinase in Schizosaccharomyces pombe that is required for cell wall integrity, and we presented genetic evidence that Pmk1/Spm1, a MAP kinase, functions downstream from Mkh1 in the same pathway. Here, we report the identification of Skh1, a MEK (MAP kinase kinase) in S. pombe. The sequence of Skh1 is nearly identical to that of the recently reported Pek1 sequence. We present biochemical and genetic evidence that Skh1 is the MEK component of the Mkh1-Spm1 MAP kinase cascade. Our yeast two-hybrid results indicate that Mkh1, Skh1, and Spm1 physically interact to form a ternary complex. Deletion of mkh1, skh1 or spm1 results in identical phenotypes, including sensitivity to (beta)-glucanase treatment, growth inhibition on media containing KCl, and filamentous growth on medium containing caffeine. Double mutant strains exhibit phenotypes that are identical to the single mutant strains. Furthermore, expression of an activated HA-Skh1(DD)protein suppressed these defects in mkh1(delta) cells, and overexpression of Spm1 suppressed these defects in skh1(delta) cells. We also show that HA-Spm1 is hyper-phosphorylated on tyrosine residues in cells co-expressing the activated HA-Skh1(DD) protein. Furthermore, we found the phosphorylated/activated form of GFP-HA-Spm1 at detectable levels in wild-type cells, but not at appreciable levels in mkh1(delta) or skh1(delta) cells expressing this fusion protein. Together, our results indicate that Mkh1, Skh1 and Spm1 constitute a MAPK cascade in fission yeast

Alternative methods in tracking sources of microbial contamination in waters

A key factor in the management and remediation of impaired ground- and surface water is the ability to distinguish the sources of faecal contamination. Several approaches have been adopted as microbial source tracking methods (MST), which are generally classified as culturing, phenotypic, genetic, and chemical MST. None of the techniques used thus far can be considered a standard; important factors, such as the statistical correlation between the source and the faecal indicator and the understanding of the environmental fate of the faecal pollutants, still need attention. The most promising MST methods available today are based on the genetic fingerprinting of faecal micro-organisms. However, research is very active also in the investigation of pharmaceuticals and personal care products discharged in the environment together with faecal waste. An updated overview of MST methods to distinguish human from animal sources of faecal pollution is presented here, focusing particularly on the potentialities of new chemical tracers

Carbonyl[hydrotris(3,5-dimethylpyrazol-1-yl)borato]copper(I) acetonitrile solvate

The title compound, [Cu(C15H22BN6)(CO)]·C2H3N, crystallizes as neutral [Tp*Cu(CO)] {[Tp*]- = hydro­tris(3,5-di­methyl­pyrazol-1-yl)­borate} molecular units and non-coordinated aceto­nitrile mol­ecules. The distorted tetrahedral coordination geometry of the copper(I) centre comprises the three N atoms of the [Tp*]- anion [Cu-N 2.033 (2)-2.054 (2) Å] and the C atom of the carbon monoxide mol­ecule [Cu-C 1.785 (4) Å]

The Actin-Binding Protein Capulet Genetically Interacts with the Microtubule Motor Kinesin to Maintain Neuronal Dendrite Homeostasis

BACKGROUND: Neurons require precise cytoskeletal regulation within neurites, containing microtubule tracks for cargo transport in axons and dendrites or within synapses containing organized actin. Due to the unique architecture and specialized function of neurons, neurons are particularly susceptible to perturbation of the cytoskeleton. Numerous actin-binding proteins help maintain proper cytoskeletal regulation. METHODOLOGY/PRINCIPAL FINDINGS: From a Drosophila forward genetic screen, we identified a mutation in capulet--encoding a conserved actin-binding protein--that causes abnormal aggregates of actin within dendrites. Through interaction studies, we demonstrate that simultaneous genetic inactivation of capulet and kinesin heavy chain, a microtubule motor protein, produces elongate cofilin-actin rods within dendrites but not axons. These rods resemble actin-rich structures induced in both mammalian neurodegenerative and Drosophila Alzheimer's models, but have not previously been identified by loss of function mutations in vivo. We further demonstrate that mitochondria, which are transported by Kinesin, have impaired distribution along dendrites in a capulet mutant. While Capulet and Cofilin may biochemically cooperate in certain circumstances, in neuronal dendrites they genetically antagonize each other. CONCLUSIONS/SIGNIFICANCE: The present study is the first molecularly defined loss of function demonstration of actin-cofilin rods in vivo. This study suggests that simultaneous, seemingly minor perturbations in neuronal dendrites can synergize producing severe abnormalities affecting actin, microtubules and mitochondria/energy availability in dendrites. Additionally, as >90% of Alzheimer's and Parkinson's cases are sporadic this study suggests mechanisms by which multiple mutations together may contribute to neurodegeneration instead of reliance on single mutations to produce disease