Simultaneous Formation of CH₃NH₃PbI₃ and electron transport layers using antisolvent method for efficient perovskite solar cells

A new antisolvent method was developed to prepare CH₃NH₃PbI₃ and electron transport layers for making efficient hybrid perovskite solar cells. By directly using [6,6]-phenyl-C61-butyric acid methyl ester in chlorobenzene solution as antisolvent, CH₃NH₃PbI₃ and electron transport layers were simultaneously formed in the films. This method not only simplifies the fabrication process of devices, but also produces uniform perovskite films and improves the interfacial structures between CH₃NH₃PbI₃ and electron transport layers. Large perovskite grains were observed in these films, with the average grain size of >1 μm. The so-formed CH₃NH₃PbI₃/electron transport layers demonstrated good optical and charge transport properties. And perovskite solar cells fabricated using these simultaneously-formed layers achieved a higher power conversion efficiency of 16.58% compared to conventional antisolvent method (14.92%). This method reduces nearly 80% usage of chlorobenzene during the fabrication, offering a more facile and environment-friendly approach to fabricate efficient perovskite solar cells than the conventional antisolvent method

Ampere-hour-scale soft-package potassium-ion hybrid capacitors enabling 6-minute fast-charging

Extreme fast charging of Ampere-hour (Ah)-scale electrochemical energy storage devices targeting charging times of less than 10 minutes are desired to increase widespread adoption. However, this metric is difficult to achieve in conventional Li-ion batteries due to their inherent reaction mechanism and safety hazards at high current densities. In this work, we report 1 Ah soft-package potassium-ion hybrid supercapacitors (PIHCs), which combine the merits of high-energy density of battery-type negative electrodes and high-power density of capacitor-type positive electrodes. The PIHC consists of a defect-rich, high specific surface area N-doped carbon nanotube-based positive electrode, MnO quantum dots inlaid spacing-expanded carbon nanotube-based negative electrode, carbonate-based non-aqueous electrolyte, and a binder- and current collector-free cell design. Through the optimization of the cell configuration, electrodes, and electrolyte, the full cells (1 Ah) exhibit a cell voltage up to 4.8 V, high full-cell level specific energy of 140 Wh kg-1 (based on the whole mass of device) with a full charge of 6 minutes. An 88% capacity retention after 200 cycles at 10 C (10 A) and a voltage retention of 99% at 25 ± 1 °C are also demonstrated

Cooperation Between Photosynthetic and Antioxidant Systems: An Important Factor in the Adaptation of Ulva prolifera to Abiotic Factors on the Sea Surface

Large-scale green tides have occurred continuously in the Yellow Sea of China from 2007 to 2018, and the causative species of the Yellow Sea green tide (YSGT) is Ulva prolifera. The thalli form floated thallus mats, and the thalli from different layers of the thallus mat suffer significantly different environmental conditions. In the present study, the environmental conditions of the surface layer (SL), middle layer (ML), and lower layer (LL) of the thallus mat from mid-June (Stage I) to mid-July (Stage II) were simulated. Photosynthetic traits and antioxidant systems were measured. The results showed that (1) photoprotective [non-photochemical quenching (NPQ) and cyclic electron transport (CEF)] and antioxidant systems both play important roles in protecting against abiotic factors in U. prolifera. (2) Cooperation between NPQ and CEF was observed in the ML group; CEF and the antioxidant system in the SL group work synergistically to protect the thalli. Furthermore, an inferred spatiotemporal attribute regarding the YSGT is presented: the significant changes in abiotic factors on the sea surface can easily affect the thalli of SL and ML from mid-June to mid-July, and those of LL can be affected in mid-July. This cooperation combined with the spatiotemporal attributes offers an explanation for the annual occurrence of the YSGT.HIGHLIGHTS    –Adaptive mechanisms of Ulva prolifera against abiotic factors.    –Cooperation between photosynthetic and antioxidant systems.    –Spatiotemporal attributes regarding the Yellow Sea green tide are presented

Dynamic distribution and expression in vivo of the human interferon gamma gene delivered by adenoviral vector

<p>Abstract</p> <p>Background</p> <p>We previously found that r-hu-IFNγ exerts a potent anti-tumor effect on human nasopharyngeal carcinoma xenografts <it>in vivo</it>. Considering the fact that the clinical use of recombinant IFNγ is limited by its short half-life and systemic side effects, we developed a recombinant adenovirus, Ad-IFNγ.</p> <p>Methods</p> <p>Dynamic distribution of the adenovirus vector and expression of IFNγ were evaluated by Q-PCR and ELISA after intratumoral administration of Ad-IFNγ into CNE-2 xenografts.</p> <p>Results</p> <p>Ad-IFNγ DNA was mainly enriched in tumors where the Ad-IFNγ DNA was injected (<it>P </it>< 0.05, compared to blood or parenchymal organs), as well as in livers (<it>P </it>< 0.05). Concentrations of Ad-IFNγ DNA in other organs and blood were very low. Intratumoral Ad-IFNγ DNA decreased sharply at high concentrations (9 × 10⁵copies/μg tissue DNA), and slowly at lower concentrations (1.7–2.9 × 10⁵copies/μg tissue DNA). IFNγ was detected in the tumors and parenchymal organs. The concentration of IFNγ was highest in the tumor (<it>P </it>< 0.05), followed by the liver and kidney (<it>P </it>< 0.05). High-level intratumoral expression of IFNγ was maintained for at least 7 days, rapidly peaking on day 3 after injection of Ad-IFNγ DNA.</p> <p>Conclusion</p> <p>An IFNγ gene delivered by an adenoviral vector achieved high and consistent intratumoral expression. Disseminated Ad-IFNγ DNA and the transgene product were mainly enriched in the liver.</p

Unregulated miR-96 Induces Cell Proliferation in Human Breast Cancer by Downregulating Transcriptional Factor FOXO3a

FOXO transcription factors are key tumor suppressors in mammalian cells. Until now, suppression of FOXOs in cancer cells was thought to be mainly due to activation of multiple onco-kinases by a phosphorylation-ubiquitylation-mediated cascade. Therefore, it was speculated that inhibition of FOXO proteins would naturally occur through a multiple step post-translational process. However, whether cancer cells may downregulate FOXO protein via an alternative regulatory mechanism is unclear. In the current study, we report that expression of miR-96 was markedly upregulated in breast cancer cells and breast cancer tissues compared with normal breast epithelial cells (NBEC) and normal breast tissues. Ectopic expression of miR-96 induced the proliferation and anchorage-independent growth of breast cancer cells, while inhibition of miR-96 reduced this effect. Furthermore, upregulation of miR-96 in breast cancer cells resulted in modulation of their entry into the G1/S transitional phase, which was caused by downregulation of cyclin-dependent kinase (CDK) inhibitors, p27Kip1 and p21Cip1, and upregulation of the cell-cycle regulator cyclin D1. Moreover, we demonstrated that miR-96 downregulated FOXO3a expression by directly targeting the FOXO3a 3′-untranslated region. Taken together, our results suggest that miR-96 may play an important role in promoting proliferation of human breast cancer cells and present a novel mechanism of miRNA-mediated direct suppression of FOXO3a expression in cancer cells

Passengers' likely behaviour based on demographic difference during an emergency evacuation in a Ro-Ro passenger ship

By examining the characteristics of passengers on a ship route between the Shandong and Liaodong Peninsula, through a questionnaire survey, this paper aims to address the likely behaviours of passengers during emergency evacuation and the demographic differences among these behaviours. A questionnaire survey of 1380 passengers shows that passengers on board are more alert and are more likely to proactively respond to evacuation alarms (62.5%), observe others’ actions (59.1%), follow evacuation instructions (67.9%), obey the crew (66.2%), queue patiently (63%), return to the cabin when their families are left behind (65.1%), and be cooperative (59%) rather than competitive (44%). The multinomial logistic regression results show that passengers who are older, with limited mobility, that have more experience aboard ships and are part of a larger group, will be more likely to proactively confirm the authenticity of evacuation events. Men, elderly individuals, people who are part of a larger group and with less experience in evacuation education are more likely to follow others. When the family is left behind, elderly individuals and people who are part of a larger group are much more likely to choose to return to their cabins. Similarly, elderly passengers with larger groups are much more likely to choose to help others. Although questionnaire research has some limitations, such as a hypothetical response and closed questions, the research results are of great significance for helping passenger ship managers to develop appropriate management rules, and conduct effective evacuation education activities

How Proactive Environmental Strategy Facilitates Environmental Reputation: Roles of Green Human Resource Management and Discretionary Slack

Based on natural resource-based theory, we examined how firms&rsquo; environmental strategies and environmental reputation depended on their green human resource management and discretionary slack. From the perspective of human resource management, we hypothesized that proactive environmental strategy predicted firms&rsquo; environmental reputation through the mediation of green human resource management. We further proposed that slack resources, which can be used discretely by managers for environmental management, enhanced the hypothesized relationships. Using data from firms in environmentally sensitive industries in eastern China, we provided empirical support for our hypotheses. The findings highlight the vital roles played by managers and green human resource management in implementing firms&rsquo; environmental strategies and promoting firms&rsquo; environmental reputation

Characteristics of Ground Pressure Disaster and Rockburst Proneness in Deep Gold Mine

AbstractTo obtain rockburst characteristics at rock engineering, the rockburst event, in situ stress characteristics, and rockburst proneness are studied in a deep gold mine. The severity of rockburst increases with cover depth at the mine. The main locations of spalling and rockburst events include roof and sidewall. The in situ stress measurement results show this area that is controlled by horizontal constructional pressure, and the self-weight stress also has an important influence. The rockburst mechanism is analyzed from the principal stress and tangential stress. When the maximum tangential stress (MTS) does not exceed 0.4 times UCS of surrounding rock, no obvious disaster in tunnel is found. When the MTS of tunnel is between about 0.4 ~ 0.55 times UCS of rock, the tunnel may suffer spalling. When the MTS of tunnel exceeds 0.55 times UCS of rock, serious failure may occur in tunnel, such as rockburst and large roof collapse. When surrounding rock is relatively hard and complete (high impact energy index, elastic strain energy index, linear elastic energy index, and RQD index), the tangential stress plays a very important role in the rockburst at the mine

The Real-Valued Sparse Direction of Arrival (DOA) Estimation Based on the Khatri-Rao Product

There is a problem that complex operation which leads to a heavy calculation burden is required when the direction of arrival (DOA) of a sparse signal is estimated by using the array covariance matrix. The solution of the multiple measurement vectors (MMV) model is difficult. In this paper, a real-valued sparse DOA estimation algorithm based on the Khatri-Rao (KR) product called the L1-RVSKR is proposed. The proposed algorithm is based on the sparse representation of the array covariance matrix. The array covariance matrix is transformed to a real-valued matrix via a unitary transformation so that a real-valued sparse model is achieved. The real-valued sparse model is vectorized for transforming to a single measurement vector (SMV) model, and a new virtual overcomplete dictionary is constructed according to the KR product’s property. Finally, the sparse DOA estimation is solved by utilizing the idea of a sparse representation of array covariance vectors (SRACV). The simulation results demonstrate the superior performance and the low computational complexity of the proposed algorithm

Failure Transition and Validity of Brazilian Disc Test under Different Loading Configurations: A Numerical Study

The Brazilian disc test is a popular tensile strength test method for engineering materials. The fracture behavior of specimens in the Brazilian disc test is closely related to the validity of the test results. In this paper, the fracture process of granite discs under different loading configurations is simulated by using a coupled finite–discrete element method. The results show that the maximum tensile stress value is located within 18 mm (0.7 times the disc radius) of the vertical range of the disc center under different loading configurations. In small diameter rods loading, the invalid tensile strength is obtained because the crack initiation and plastic strain is at the end of the disc. The crack initiation points of flat platen loading and curved jaws loading are all within the center of the disc, and the valid tensile strength can be obtained. The tensile strength test results under different loading configurations show that the error of small diameter rods loading is 13%, while the errors of flat platen loading and curved jaws loading are both 1%. The curved jaws loading is the most suitable for measuring the tensile strength of brittle materials such as rock, followed by flat platen loading. The small diameter rods loading is not recommended for the Brazilian test