Tissue-specific alternative splicing and expression of ATP1B2 gene

The Na+-K+-ATPase is an essential transport enzyme expressed in all animal tissues, where it generates ion gradients to maintain membrane potential and drive the transport of other solutes. It also balances metabolism and body temperature. In this study, the characterization of three novel bovine ATP1B2 splice variants, designated as ATP1B2-AS1, ATP1B2-AS2, and ATP1B2-AS3, is discussed. All three novel splice isoforms were derived from a complete transcript (ATP1B2-complete) by alternative splicing. The pattern of splicing to produce the ATP1B2-AS1 and ATP1B2-AS2 isoforms was intron retention; these isoforms were found in liver, kidney, muscle and breast tissues. For the ATP1B2-AS3 isoform, splicing was by exon inclusion and this isoform was only found in muscle tissue. As demonstrated by real-time polymerase chain reaction, the isoforms were all expressed at significantly lower levels than the complete ATP1B2 gene transcript in all the tissues studied. After heat-stress, the expression levels of the different transcripts were lower in different tissues; however, the expression of the ATP1B2-complete transcript increased in heart and lung tissues. The results of this research provide some useful information for further studies into the function of the bovine ATP1B2 gene. Alternative splicing (AS) is recognized as the major contributor to protein diversity from limited gene pool. ATP1B2-AS2 was the splice of intron retention found from ATP1B2 in liver, kidney, muscle and breast tissues. In the study, ATP1B2-AS2 showed that many of the amino acid residues were in an unfavorable energy environment. It is interesting to speculate that this may be the perfect transcript to respond to heat-stress. So, AS may become the appropriate pathway to tackle heat-stress and reduce the economic losses in cows.Key words: ATP1B2 gene, alternative splicing, alternative splicing mechanism

Living Condition and Willingness of Migrant Workers – A Case Study of Panyu District in Guangzhou City

Migrant worker is the product of urban and rural dual structure, and also an important force promoting social development. Paying close attention to living condition and willingness of migrant workers is favorable for improving people’s livelihood and stabilizing society. Panyu District of Guangzhou City has gathered numerous migrant workers. Therefore, it randomly selected 600 migrant workers in Panyu District and took them as objects of questionnaire. Through research, it is found that current living condition of migrant workers is not optimistic, and it is expected that their housing problem can be solved

The Relationship between Nutrition Knowledge and Nutrition Facts Table Use in China: A Structural Equation Model

Since 2013, China has implemented a nutrition label regulation that aims to provide essential nutrition information through nutrition facts tables labeled on the back of food packages. Yet, the relationship between people’s nutrition knowledge and their nutrition label use remains less clear. This study adopted the structural equation modeling approach to analyze a nationally representative survey of 1500 Chinese individuals through the cognitive processing model, interrelated nutrition knowledge, attention to nutrition information on the nutrition facts table, comprehension of nutrition information, food choice and dietary intake. It was found that nutrition knowledge positively influenced attention to nutrition information; a better comprehension of nutrition information, which could benefit healthier food choices, did not relate to a higher level of attention to that information; dietary intake was affected significantly by nutrition knowledge, but it had little impact on food choice. The results signify that nutrition knowledge hardly supports nutrition facts table use among the Chinese people, mainly due to incomprehensible labeled information. Therefore, it emphasizes the need to enhance people’s comprehension through front-of-package labels and corresponding smartphone applications

The value co-creation in automotive-3PL relationship management : A case study in Sweden

Background: The discussion regarding value co-creation begins within the field of service marketing, it tells that the value is mutually created in process between the firms and the customers. And that concept has been developed further in many aspects, such as B2B context in which the knowledge intensive industry is involved. Many researchers have argued the importance of value co-creation and emphasized on the need of more studies within business practices. In this study, the particular automotive-3PL relationship would be added in to explore the empirical study of value co-creation. Purpose: The main purpose of the thesis is to study how value co-creation can be managed and achieved from the perspectives of 3PL providers and the users within automotive industry. Method: Through the qualitative research approach, the comprehensive knowledge, which includes the 3PL providers and users’ insights and undertaken behaviours behind the value co-creation is obtained after conducting eight semi-structured interviews in a case study. Conclusion: The results show that the value co-creation is actualized when 3PL providers and automotive users together actively participate in a certain process, in which the offering, interaction and learning are the core activities that should be maintained circularly. Furthermore, the high level of relationship commitment and trust should be sustained between actors

Analysis of Chinese Consumers’ Nutrition Facts Table Use Behavior Based on Knowledge-Attitude-Practice Model

The nutrition facts table is a nutrition labeling tool designed to help consumers understand the nutritional content of food and make informed food choices. However, the utilization rate of the nutrition facts table has been low in China since its introduction in 2013. This study employed structural equation modeling to investigate consumers’ knowledge, attitudes, and practices related to the nutrition facts table by using an online survey of 930 valid samples from 31 provinces in China based on the knowledge, attitude, practice (KAP) model. The analysis revealed that most respondents had a positive attitude, but not a good level of knowledge or practice in their use of the labeling. Respondents’ knowledge about the nutrition facts table directly affected their attitude, through which it could then affect their practice, whereas knowledge played an indirect role in nutrition labeling use. The results confirmed that the KAP model is suitable for explaining consumers’ use of nutrition labels in China, and that the first step to promoting labeling usage is to enrich consumers’ knowledge on the nutrition facts table

Factors Affecting Salt Reduction Measure Adoption among Chinese Residents

China has the highest mortality rate caused by diseases and conditions associated with its high-salt diet. Since 2016, China has initiated a national salt reduction campaign that aims at promoting the usage of salt information on food labels and salt-restriction spoons and reducing condiment and pickled food intake. However, factors affecting individuals’ decisions to adopt these salt reduction measures remain largely unknown. By comparing the performances of logistic regression, stepwise logistic regression, lasso logistic regression and adaptive lasso logistic regression, this study aims to fill this gap by analyzing the adoption behaviour of 1610 individuals from a nationally representative online survey. It was found that the practices were far from adopted and only 26.40%, 22.98%, 33.54% and 37.20% reported the adoption of labelled salt information, salt-restriction spoons, reduced condiment use in home cooking and reduced pickled food intake, respectively. Knowledge on salt, the perceived benefits of salt reduction, participation in nutrition education and training programs on sodium reduction were positively associated with using salt information labels. Adoption of the other measures was largely explained by people’s awareness of hypertension risks and taste preferences. It is therefore recommended that policy interventions should enhance Chinese individuals’ knowledge of salt, raise the awareness of the benefits associated with a low-salt diet and the risks associated with consuming excessive salt and reshape their taste choices

Determinants of Nutrition Facts Table Use by Chinese Consumers for Nutritional Value Comparisons

The nutrition facts table is a nutrition labeling tool designed to inform consumers of food nutritional contents and enable them to make healthier choices by comparing the nutritional values of similar foods. However, its adoption level is considerably low in China. This study employed the Chi-squared Automatic Interaction Detection (CHAID) algorithm to explore the factors associated with respondents’ adoption of nutrition facts table to compare the nutritional values of similar foods. Data were gathered through a nationally representative online survey of 1500 samples. Results suggested that consumers’ comprehension of the nutrition facts table was a direct explanatory factor for its use. The usage was also indirectly explained by people’s nutrition knowledge, the usage of nutrition facts table by their relatives and friends, and their focus on a healthy diet. Therefore, to increase the use of nutrition facts table by Chinese consumers, the first consideration should be given to enhancing consumers’ comprehension of the labelin

Chinese Adults’ Willingness to Pay for Mandatory Nutrients Reporting on Nutrition Facts Table

The Chinese food industry has opposed the mandatory inclusion of increased nutrients in the Nutrition Facts Table (NFT), thus impeding its improvement. This poses a challenge to the endeavors aiming to assist consumers in cultivating healthy dietary habits that incorporate reduced saturated fatty acids and added sugars while ensuring the adequate intake of essential micronutrients. This study conducted a choice experiment to investigate Chinese consumers’ preference for updated labeling schemes among 630 adults that were randomly selected from Central, North, East, South, Northwest, Southwest, and Northeast China. It revealed that respondents were willing to pay the highest premium for the most mandatory nutrients (22.575% of the food price per unit). Respondents preferred the NFT with the most mandatory nutrients if they met the following population characteristics: female; non-overweight or obese; without a college degree; possessed an annual household disposable income between 50,000 and 99,999 CNY; from North China; lived in rural areas and often cooked for family; cared about food nutrition. Two combinations of NFT information received the highest preference: (1) the NFT detailing the most mandatory nutrients and their content values and nutrient reference values (NRV%); (2) the NFT containing the most nutrients and the nutrients in 100 g/mL or a serving. The first and second combinations attracted a premium of 14.884% and 31.833% of the food price per unit, respectively

Sustainable Healthy Diets and Demand for the Front-of-Package Labeling: Evidence from Consumption of Fresh Pork

Front of Package (FOP) labeling, which assists consumers in understanding the nutritional status of fresh pork, could help reduce Chinese residents’ excessive meat intake and greenhouse gas emissions. Nevertheless, little is known about the price elasticity of consumers’ demand for the FOP labeling on fresh pork. This study implemented a contingent valuation survey by using a representative survey of 930 adults across China. The results indicated that respondents’ demand for FOP labeling applied to fresh pork was price inelastic (−0.209). Moreover, there existed significant population heterogeneity in the price elasticity of demand. Specifically, WTP increased for the urban population and those who paid regular attention to pork’s nutritional value. Overall, Chinese consumers had rigid demand for FOP labeling on fresh pork, and policy makers should pay close attention to consumer reactions to the price of FOP labeling and promote this application on fresh pork