Investigating Glucose Uptake Prognostic Effects on Pan Cancer TCGA by METAFlux

https://openworks.mdanderson.org/sumexp22/1135/thumbnail.jp

A Structured Table of Graphs with Symmetries and Other Special Properties

We organize a table of regular graphs with minimal diameters and minimal mean path lengths, large bisection widths and high degrees of symmetries, obtained by enumerations on supercomputers. These optimal graphs, many of which are newly discovered, may find wide applications, for example, in design of network topologies.Comment: add details about automorphism grou

Regulatory controls of duplicated gene expression during fiber development in allotetraploid cotton.

Polyploidy complicates transcriptional regulation and increases phenotypic diversity in organisms. The dynamics of genetic regulation of gene expression between coresident subgenomes in polyploids remains to be understood. Here we document the genetic regulation of fiber development in allotetraploid cotton Gossypium hirsutum by sequencing 376 genomes and 2,215 time-series transcriptomes. We characterize 1,258 genes comprising 36 genetic modules that control staged fiber development and uncover genetic components governing their partitioned expression relative to subgenomic duplicated genes (homoeologs). Only about 30% of fiber quality-related homoeologs show phenotypically favorable allele aggregation in cultivars, highlighting the potential for subgenome additivity in fiber improvement. We envision a genome-enabled breeding strategy, with particular attention to 48 favorable alleles related to fiber phenotypes that have been subjected to purifying selection during domestication. Our work delineates the dynamics of gene regulation during fiber development and highlights the potential of subgenomic coordination underpinning phenotypes in polyploid plants. [Abstract copyright: © 2023. The Author(s).

Pathway centric analysis for single-cell RNA-seq and spatial transcriptomics data with GSDensity

Abstract Advances in single-cell technology have enabled molecular dissection of heterogeneous biospecimens at unprecedented scales and resolutions. Cluster-centric approaches are widely applied in analyzing single-cell data, however they have limited power in dissecting and interpreting highly heterogenous, dynamically evolving data. Here, we present GSDensity, a graph-modeling approach that allows users to obtain pathway-centric interpretation and dissection of single-cell and spatial transcriptomics (ST) data without performing clustering. Using pathway gene sets, we show that GSDensity can accurately detect biologically distinct cells and reveal novel cell-pathway associations ignored by existing methods. Moreover, GSDensity, combined with trajectory analysis can identify curated pathways that are active at various stages of mouse brain development. Finally, GSDensity can identify spatially relevant pathways in mouse brains and human tumors including those following high-order organizational patterns in the ST data. Particularly, we create a pan-cancer ST map revealing spatially relevant and recurrently active pathways across six different tumor types

Optimisation of mobile intelligent terminal data pre-processing methods for crowd sensing

Sensor data pre-processing is an essential phase of crowd sensing application. Existing studies do not effectively solve the problem, and there still exist various sensor data pre-processing optimisation problems at the acquisition end in crowd-sensing process. This study presents an improved sliding average method to achieve data compression and reduce the time complexity by using a dynamic window with improved processing time. Through adopting locally sorting and gradient change of the filter window, an improved extremum median filtering method is proposed to relieve the time-consuming problem when denoising high pixel images. A transmission strategy for optimisation is also proposed, in which only the demarcation points of each group of data and the data points with large differences when comparing with the demarcation points are recorded. This strategy reduces the storage pressure and the amount of data transmission of mobile terminal and improves the efficiency of data transmission. The experimental results show that their methods have higher speed and lower cost, and thus they can run better in crowd-sensing environment

A New Record of Graph Enumeration Enabled by Parallel Processing

Using three supercomputers, we broke a record set in 2011, in the enumeration of non-isomorphic regular graphs by expanding the sequence of A006820 in the Online Encyclopedia of Integer Sequences (OEIS), to achieve the number for 4-regular graphs of order 23 as 429,668,180,677,439, while discovering several regular graphs with minimum average shortest path lengths (ASPL) that can be used as interconnection networks for parallel computers. The enumeration of 4-regular graphs and the discovery of minimal-ASPL graphs are extremely time consuming. We accomplish them by adapting GENREG, a classical regular graph generator, to three supercomputers with thousands of processor cores

Characterization of In Vivo Protein Complexes via Chemical Cross-Linking and Mass Spectrometry

Cells perform various functions by proteins via protein complexes. Characterization of protein complexes is critical to understanding their biological and clinical significance and has been one of the major efforts of functional proteomics. To date, most protein complexes are characterized by the in vitro system from protein extracts after the cells or tissues are lysed, and it has been challenging to determine which of these protein complexes are formed in intact cells. Herein, we report an approach to preserve protein complexes using in vivo cross-linking, followed by size exclusion chromatography and data-independent acquisition mass spectrometry. This approach enables the characterization of in vivo protein complexes from cells or tissues, which allows the determination of protein complexes in clinical research. More importantly, the described approach can identify protein complexes that are not detected by the in vitro system, which provide unique protein function information