405 research outputs found
Chiral structured illumination microscopy: a microscopy modality for imaging fluorescent chiral domains at sub-wavelength resolution
This thesis covers the development of chiral structured illumination microscopy (chiral SIM), a novel wide-field super-resolution microscopy that is able to address the shortcomings restricting the capability of conventional chiral imaging techniques. Ac- cordingly, the research background and fundamentals in regards to this new microscopy method are introduced at the beginning. The subsequent chapters illustrate the de- velopment of chiral SIM in both theoretical and experimental aspects. In particular, we establish the theoretical framework of chiral SIM and investigate possible far-field illumination schemes for its experimental realization. The proof-of-principle numerical simulations demonstrate its ability to image and discriminate chiral domains at super- resolution. The signal-to-noise ratio of the chiral SIM images simulated under different experimental conditions is analyzed to inspect the performance of chiral SIM. Meanwhile, we study the near-field illumination schemes that exploit the superchiral near fields generated by plasmonic or dielectric nanostructures to reinforce the chiroptical responses and elaborate on the main difficulty of applying them in the proposed chiral SIM method. After a series of theoretical work, we present our experimental attempts at the chiral SIM method. The fabrication and inspection of the selected chiral sample and the optical setups are specifically discussed. Although the experimental results are unsatisfactory, based on the following investigation, we further propose a new optical setup to solve the potential issues of the original chiral SIM setup. Finally, we summarize this thesis and provide suggestions that should be beneficial to the subsequent research in chiral SIM and may also open up new research fields
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Signal and noise analysis for chiral structured illumination microscopy
Recently, chiral structured illumination microscopy has been proposed to image fluorescent chiral domains at sub-wavelength resolution. Chiral structured illumination microscopy is based on the combination of structured illumination microscopy, fluorescence-detected circular dichroism, and optical chirality engineering. Since circular dichroism of natural chiral molecules is typically weak, the differential fluorescence is also weak and can be easily buried by the noise, hampering the fidelity of the reconstructed images. In this work, we systematically study the impact of the noise on the quality and resolution of chiral domain images obtained by chiral SIM. We analytically describe the signal-to-noise ratio of the reconstructed chiral SIM image in the Fourier domain and verify our theoretical calculations with numerical demonstrations. Accordingly, we discuss the feasibility of chiral SIM in different experimental scenarios and propose possible strategies to enhance the signal-to-noise ratio for samples with weak circular dichroism
Signal and Noise Analysis for Chiral Structured Illumination Microscopy
Recently, chiral structured illumination microscopy has been proposed to
image fluorescent chiral domains at sub-wavelength resolution. Chiral
structured illumination microscopy is based on the combination of structured
illumination microscopy, fluorescence-detected circular dichroism, and optical
chirality engineering. Since circular dichroism of natural chiral molecules is
typically weak, the differential fluorescence is also weak and can be easily
buried by the noise, hampering the fidelity of the reconstructed images. In
this work, we systematically study the impact of the noise on the quality and
resolution of chiral domain images obtained by chiral SIM. We analytically
describe the signal-to-noise ratio of the reconstructed chiral SIM image in the
Fourier domain and verify our theoretical calculations with numerical
demonstrations. Accordingly, we discuss the feasibility of chiral SIM in
different experimental scenarios and propose possible strategies to enhance the
signal-to-noise ratio for samples with weak circular dichroism.Comment: 18 page
Generation of Optical Chirality Patterns with Plane Waves, Evanescent Waves and Surface Plasmon Waves
We systematically investigate the generation of optical chirality patterns by
applying the superposition of two waves in three scenarios, namely plane waves
in free space, evanescent waves of totally reflected light at dielectric
interface and propagating surface plasmon waves on a metallic surface. In each
scenario, the general analytical solution of the optical chirality pattern is
derived for different polarization states and propagating directions of the two
waves. The analytical solutions are verified by numerical simulations.
Spatially structured optical chirality patterns can be generated in all
scenarios if the incident polarization states and propagation directions are
correctly chosen. Optical chirality enhancement can be obtained from the
constructive interference of free-space circularly polarized light or enhanced
evanescent waves of totally reflected light. Surface plasmon waves do not
provide enhanced optical chirality unless the near-field intensity enhancement
is sufficiently high. The structured optical chirality patterns may find
applications in chirality sorting, chiral imaging and circular dichroism
spectroscopy
Beyond the Innovation: An Exploratory Study of Designing Web-based Self-services
AbstractCustomized web-based self-services play an important role in today's product/service innovation. Compared to traditional tangible services, helpful web-based self-services and off-line services may better facilitate creativity, accelerate value co-creation, and reduce the costs and risks of development and commercialization. Therefore, in order to offer a conceptual framework for a web-based self-service system that enhances the fuzzy-front end (FFE) of new product/service development, this study analyzed the needs and challenges found during the transition of the Dechnology (Design Thinking plus Technology Innovation) project at the Industrial Technology Research Institute (ITRI), the largest R&D organization in Taiwan. Through literature review, in-depth interviews, and participatory action research, we formalized five core system modules, including: 1) user behavior and lifestyle, 2) thematic trend analysis, 3) technology screening and translation, 4) idea visualization, and 5) O2O service connection, with corresponding design principles for supporting user creativity in a web-based self-services environment. Finally, this study proposes a conceptual framework integrated with service design to serve as an important reference for enterprises that undergo similar innovation projects in the future
Foam Properties and Detergent Abilities of the Saponins from Camellia oleifera
The defatted seed meal of Camellia oleifera has been used as a natural detergent and its extract is commercially utilized as a foam-stabilizing and emulsifying agent. The goal of this study was to investigate the foam properties and detergent ability of the saponins from the defatted seed meal of C. oleifera. The crude saponin content in the defatted seed meal of C. oleifera was 8.34 and the total saponins content in the crude saponins extract was 39.5% (w/w). The foaming power of the 0.5 crude saponins extract solution from defatted seed meal of C. oleifera was 37.1 of 0.5 SLS solution and 51.3% to that of 0.5% Tween 80 solution. The R5 value of 86.0% represents good foam stability of the crude saponins extracted from the defatted seed meal of the plant. With the reduction of water surface tension from 72 mN/m to 50.0 mN/m, the 0.5% crude saponins extract solution has wetting ability. The sebum-removal experiment indicated that the crude saponins extract has moderate detergency. The detergent abilities of the saponins from C. oleifera and Sapindus mukorossi were also compared
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Metabolic labelling of cholesteryl glucosides in Helicobacter pylori reveals how the uptake of human lipids enhances bacterial virulence.
Helicobacter pylori infects approximately half of the human population and is the main cause of various gastric diseases. This pathogen is auxotrophic for cholesterol, which it converts upon uptake to various cholesteryl α-glucoside derivatives, including cholesteryl 6'-acyl and 6'-phosphatidyl α-glucosides (CAGs and CPGs). Owing to a lack of sensitive analytical methods, it is not known if CAGs and CPGs play distinct physiological roles or how the acyl chain component affects function. Herein we established a metabolite-labelling method for characterising these derivatives qualitatively and quantitatively with a femtomolar detection limit. The development generated an MS/MS database of CGds, allowing for profiling of all the cholesterol-derived metabolites. The subsequent analysis led to the unprecedented information that these bacteria acquire phospholipids from the membrane of epithelial cells for CAG biosynthesis. The resulting increase in longer or/and unsaturated CAG acyl chains helps to promote lipid raft formation and thus delivery of the virulence factor CagA into the host cell, supporting the idea that the host/pathogen interplay enhances bacterial virulence. These findings demonstrate an important connection between the chain length of CAGs and the bacterial pathogenicity
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