Identification of QuiP, the Product of Gene PA1032, as the Second Acyl-Homoserine Lactone Acylase of Pseudomonas aeruginosa PAO1

The relevance of the acyl homoserine lactone (acyl-HSL) quorum signals N-3-oxododecanoyl-homoserine lactone (3OC12HSL) and N-butanoyl-homoserine lactone to the biology and virulence of Pseudomonas aeruginosa is well investigated. Previously, P. aeruginosa was shown to degrade long-chain, but not short-chain, acyl-HSLs as sole carbon and energy sources (J. J. Huang, J.-I. Han, L.-H. Zhang, and J. R. Leadbetter, Appl. Environ. Microbiol. 69:5941-5949, 2003). A gene encoding an enzyme with acyl-HSL acylase activity, pvdQ (PA2385), was identified, but it was not required for acyl-HSL utilization. This indicated that P. aeruginosa encodes another acyl-HSL acylase, which we identify here. A comparison of total cell proteins of cultures grown with long-acyl acyl-HSLs versus other substrates implicated the involvement of a homolog of PvdQ, the product of gene PA1032, for which we propose the name QuiP. Transposon mutants of quiP were defective for growth when P. aeruginosa was cultured in medium containing decanoyl-HSL as a sole carbon and energy source. Complementation with a functional copy of quiP rescued this growth defect. When P. aeruginosa was grown in buffered lysogeny broth, constitutive expression of QuiP in P. aeruginosa led to decreased accumulations of the quorum signal 3OC12HSL, relative to the wild type. Heterologous expression of QuiP was sufficient to confer long-chain acyl-HSL acylase activity upon Escherichia coli. Examination of gene expression patterns during acyl-HSL-dependent growth of P. aeruginosa further supported the involvement of quiP in signal decay and revealed other genes also possibly involved. It is not yet known under which "natural" conditions quiP is expressed or how P. aeruginosa balances the expression of its quorum-sensing systems with the expression of its acyl-HSL acylase activities

Existence and uniqueness for Mean Field Games with state constraints

In this paper, we study deterministic mean field games for agents who operate in a bounded domain. In this case, the existence and uniqueness of Nash equilibria cannot be deduced as for unrestricted state space because, for a large set of initial conditions, the uniqueness of the solution to the associated minimization problem is no longer guaranteed. We attack the problem by interpreting equilibria as measures in a space of arcs. In such a relaxed environment the existence of solutions follows by set-valued fixed point arguments. Then, we give a uniqueness result for such equilibria under a classical monotonicity assumption

The Effect of Plant Water Storage on Water Fluxes within the Coupled Soil–Plant System

In addition to buffering plants from water stress during severe droughts, plant water storage (PWS) alters many features of the spatio-temporal dynamics of water movement in the soil–plant system. How PWS impacts water dynamics and drought resilience is explored using a multi-layer porous media model

Evidence for mechanisms underlying the functional benefits of a myocardial matrix hydrogel for post-MI treatment

Background There is increasing need for better therapies to prevent the development of heart failure after myocardial infarction (MI). An injectable hydrogel derived from decellularized porcine ventricular myocardium has been shown to halt the post-infarction progression of negative left ventricular remodeling and decline in cardiac function in both small and large animal models. Objectives This study sought to elucidate the tissue-level mechanisms underlying the therapeutic benefits of myocardial matrix injection. Methods Myocardial matrix or saline was injected into infarcted myocardium 1 week after ischemia-reperfusion in Sprague-Dawley rats. Cardiac function was evaluated by magnetic resonance imaging and hemodynamic measurements at 5 weeks after injection. Whole transcriptome microarrays were performed on RNA isolated from the infarct at 3 days and 1 week after injection. Quantitative polymerase chain reaction and histologic quantification confirmed expression of key genes and their activation in altered pathways. Results Principal component analysis of the transcriptomes showed that samples collected from myocardial matrix-injected infarcts are distinct and cluster separately from saline-injected control subjects. Pathway analysis indicated that these differences are due to changes in several tissue processes that may contribute to improved cardiac healing after MI. Matrix-injected infarcted myocardium exhibits an altered inflammatory response, reduced cardiomyocyte apoptosis, enhanced infarct neovascularization, diminished cardiac hypertrophy and fibrosis, altered metabolic enzyme expression, increased cardiac transcription factor expression, and progenitor cell recruitment, along with improvements in global cardiac function and hemodynamics. Conclusions These results indicate that the myocardial matrix alters several key pathways after MI creating a pro-regenerative environment, further demonstrating its promise as a potential post-MI therapy

Polynomial Structure of the (Open) Topological String Partition Function

In this paper we show that the polynomial structure of the topological string partition function found by Yamaguchi and Yau for the quintic holds for an arbitrary Calabi-Yau manifold with any number of moduli. Furthermore, we generalize these results to the open topological string partition function as discussed recently by Walcher and reproduce his results for the real quintic.Comment: 15 page

Strangeness and the discovery of quark-gluon plasma

Strangeness flavor yield s and the entropy yield S are the observables of the deconfined quark-gluon state of matter which can be studied in the entire available experimental energy range at AGS, SPS, RHIC, and, in near future, at the LHC energy range. We present here a comprehensive analysis of strange, soft hadron production as function of energy and reaction volume. We discuss the physical properties of the final state and argue how evidence about the primordial QGP emerges.Comment: 16 pages: Invited talk at 5th International Conference on Physics and Astrophysics of Quark Gluon Plasma, February 8 - 12, 2005, Salt Lake City, Kolkata, India, to appear in: Journal of Physics: Conference Serie

OSSOS. XXIX. The Population and Perihelion Distribution of the Detached Kuiper Belt

The detached transneptunian objects (TNOs) are those with semimajor axes beyond the 2:1 resonance with Neptune, which are neither resonant nor scattering. Using the detached sample from the OSSOS telescopic survey, we produce the first studies of their orbital distribution based on matching the orbits and numbers of the known TNOs after accounting for survey biases. We show that the detached TNO perihelion ($q$) distribution cannot be uniform, but is instead better matched by two uniform components with a break near $q\approx40$ au. We produce parametric two-component models that are not rejectable by the OSSOS data set, and estimate that there are $36,\!000^{+12,000}_{-9,000}$ detached TNOs with absolute magnitudes $H_r < 8.66$ ($D \gtrsim 100$ km) and semimajor axes $48 < a < 250$ au (95% confidence limits). Although we believe these heuristic two-parameter models yield a correct population estimate, we then use the same methods to show that the perihelion distribution of a detached disk created by a simulated rogue planet matches the $q$ distribution even better, suggesting that the temporary presence of other planets in the early Solar System is a promising model to create today's large semimajor axis TNO population. This numerical model results in a detached TNO population estimate of $48,\!000^{+15,000}_{-12,000}$. Because this illustrates how difficult-to-detect $q>50$ au objects are likely present, we conclude that there are $(5 \pm 2)\times10^4$ dynamically detached TNOs, which are thus roughly twice as numerous as the entire transneptunian hot main belt.Comment: Accepted for publication in The Planetary Science Journal. 16 pages, 8 figure

Chiral, air stable, and reliable Pd(0) precatalysts applicable to asymmetric allylic alkylation chemistry.

Stereoselective carbon-carbon bond formation via palladium-catalyzed asymmetric allylic alkylation is a crucial strategy to access chiral natural products and active pharmaceutical ingredients. However, catalysts based on the privileged Trost and Pfaltz-Helmchen-Williams PHOX ligands often require high loadings, specific preactivation protocols, and excess chiral ligand. This makes these reactions uneconomical, often unreproducible, and thus unsustainable. Here we report several chiral single-component Pd(0) precatalysts that are active and practically-applicable in a variety of asymmetric allylic alkylation reactions. Despite the decades-long history and widespread use of Trost-type ligands, the precatalysts in this work are the only reported examples of stable, isolable Pd(0) complexes with these ligands. Evaluating these precatalysts across nine asymmetric allylic alkylation reactions reveals high reactivity and selectivity at low Pd loading. Importantly, we also report an unprecedented Pd-catalyzed enantioselective allylation of a hydantoin, achieved on gram scale in high yield and enantioselectivity with only 0.2 mol% catalyst

Conservation laws arising in the study of forward-forward Mean-Field Games

We consider forward-forward Mean Field Game (MFG) models that arise in numerical approximations of stationary MFGs. First, we establish a link between these models and a class of hyperbolic conservation laws as well as certain nonlinear wave equations. Second, we investigate existence and long-time behavior of solutions for such models