Additional file 1 of OTUD4-mediated GSDME deubiquitination enhances radiosensitivity in nasopharyngeal carcinoma by inducing pyroptosis

Additional file 1: Figure S1. Radiotherapy induces GSDME-dependent pyroptosis in NPC cells through the intrinsic mitochondrial apoptotic pathway. Figure S2. Upregulation of GSDME enhances pyroptosis and radiosensitivity in NPC cells in vitro. Figure S3. Low GSDME expression correlates with radioresistance and poor prognosis in NPC. Figure S4. No significant difference is found in the mRNA expression of GSDME between radiosensitive and radioresistant NPC specimens. Figure S5. Knockdown GSDME significantly reverses cell dead promotion induced by overexpression OTUD4 after irradiation. Figure S6. Upregulating or silencing OTUD4/GSDME results in no significant difference in xenograft tumor growth in the absence of ionizing radiation. Figure S7. Upregulating OTUD4 increases oligomerization level of GSDME-N in NPC cells after irradiation, which is reversed by GSDME knockdown. Figure S8. Percentage of OTUD4 low expression and high expression in NPC tissues. Table S1. Clinicopathological characteristics and tumor-specific expression of GSDME in NPC. Table S2. Univariate and multivariate analysis of factors associated with PFS and LRRFS in 150 NPC patients. Table S3. Relationship between OTUD4 expression and patient clinicopathological features. Table S4. shRNA target sequences used in this study