Bacterial dysbiosis risk prediction according to vaginal normobiota indicator

Vaginal bacterial dysbiosis is evident by the progressive growth of obligate and optional anaerobic bacteria, myco- and ureaplasmas and fungi against the background of a sharp decrease in the number of lactobacilli. The aim of those work was to establish prognostic factors and the risk of bacterial dysbiosis development according to the vaginal normobiota indicator based on the comprehensive analysis of the state of systemic and local immunity and hormonal regulation. As conclusions, with bacterial dysbiosis the pathological regulatory hormonal-immune system is formed, which contributes to the vaginal dysbiosis progression. In accordance with this, dysbiosis can be considered as a dysregulatory pathology, and the identified indicators of the “interleukin cascade” as its markers

Modulation of three-dimensional structure and research of folding-analoges of AMB A 6 allergen of Ambrosia artemisiifolia

Amb A 6 allergen of Ambrosia artemisiifolia is a ragweed allergen (a principle cause of late summer hayfever in North America and Europe) (Hiller et al. 1998). The weed has recently become spreading as a neophyte in Europe, while climate change may also affect the growth of the plant and additionally may also influence pollen allergenicity (Kelish et al. 2014). In Ukraine, the number of diseases caused by this allergen has recently increases. The three-dimensional structure of Amb A 6 allergen is undescribed. The aim of our study was to modulate of three-dimensional structure and search of folding-analoges of AMB A 6 allergen of A. artemisiifolia

The order of duplication of human aldh encoding gene

Human aldehyde dehydrogenase (ALDH) causes oxidation of acetaldehyde to acetate. Humans have 18 genes encoding the ALDH enzymes. The order of duplication of human ALDH encoding gene was not completely detected. In this work ALDH encoding gene was analyzed by phylogenetic methods. Genetic distances of paralogs and order of duplication of human ALDH encoding gene were detected

Molecular size determination of hyaluronic acid

Hyaluronic acid (HA) is polysaccharide widely used in the medicine. In the usage of HA the size of molecules has a very important signifcance. Identifcation of HA size is usually carried out by paper chromatography method or by fltration through micropores with markers of molecular size. However, the mentioned methods are very expensive and need extra time to be completed. Te aim of scientifc research was to detect the possibility of separation and identifcation of molecular size of HA by electrophoresis in the polyacrylamide gel. Possibility to separate and identifcate the specifc size of HA molecules was shown in polyacrylamide gel by electrophoretic method. Tis method requires less time and resources than ones which are usually used and can be applied both in industry and medicine

Investigations of the antimicrobial activity of aminomethanesulfonic acids against strains of Staphylococcus aureus with different antimicrobial susceptibility

One of the tasks of the WHO strategy against development of antibiotic resistance in microorganisms is the searching for new compounds with antimicrobial activity to develop new antimicrobial medicines. The aim of this study was to determine the inhibitory effect of aminosulfonic acid (AMSA) and its new derivatives such as N-methyl-(MeAMSA), N-(2-hydroxyethyl)-(HEAMSA), N-benzyl-(BnAMSA), N-(tert-butyl)-(t-BuAMSA), 4-(N-phenylaminomethyl)phenyl (PhAMPhAMSA) on the growth of Staphylococcus aureus strains with different antimicrobial susceptibility. Materials and methods. The method of serial dilution was used in the study. The chemical compounds were dissolved in DMSO (a final concentration of 1%). Then dilutions of the compounds were performed using liquid Mueller-Hinton medium to final concentrations of 5 mM and 10 m×M. The results were assessed using a Densi-La-Meter after 18-20 hours of incubation at 37 °C. Sulfanilamide was used as a reference preparation. Results. AMSA suppressed the growth of all tested strains regardless of their antibiotic resistance profiles even more than sulfanilamide. MeAMSA inhibited the growth of S. aureus ATCC 25923 and S. aureus 2781 strains more than the reference preparation, but less than AMSA. No inhibitory effect was observed on the antibiotic resistant S. aureus Kunda strain. Compounds of HEAMSA, t-BuAMSA, BnAMSA stably inhibited the growth of all strains tested. These compounds suppressed the growth of S. aureus ATCC 25923 and S. aureus Kunda strains more than sulfanilamide. However, the reference preparation exhibited greater S. aureus 2781 growth inhibition than investigated preparations. Compound PhAMPhAMSA did not show antimicrobial activity. Conclusions. Aminomethanesulfonic acid derivatives suppressed the growth of Staphylococcus aureus strains with different antimicrobial susceptibility and their antimicrobial activity was higher than that of sulfanilamide. Further study of these compounds efficacy on different types of microorganisms can be considered promising for the development of new antimicrobial agents. It would also be appropriate to study the combined use of these substances with antibiotics

The use of sensory deprivation in patients with long-term consequences of mild combat traumatic brain injury

Актуальність. Черепно-мозкова травма (ЧМТ) та її наслідки нерідко призводять до інвалідизації, прогресування як самих синдромів, що виникають після травми, так і коморбідної патології. Резистентність до терапії диктує пошук нових патогенетичних підходів до лікування даної патології. Особливо скрутна діагностика легких уражень головного мозку, через компенсацію на ранніх етапах низьких станів, ігнорування наявних симптомів з подальшою неадекватністю лікувальних заходів, що призводить до стійких порушень механізмів регуляції та адаптації. Ціль: вивчення впливу сенсорної депривації на динаміку показників вегетативної нервової системи (ВНС), її нейрогормональної ланки та гормону мелатоніну у хворих із наслідками легкої бойової ЧМТ. Матеріали та методи. Обстежено 53 пацієнти з наслідками легкої мінно-вибухової ЧМТ, які лікувалися методом сенсорної депривації у спеціальній флоат-камері. Курс лікування складав 10-12 сеансів по 45-60 хвилин на день. Досліджували неврологічний статус, стан ВНС: вегетативний тонус, реактивність, нейрогормональну ланку (адреналін, норадреналін, мелатонін), мікроциркуляцію у судинах бульбарної кон’юнктиви. Обстеження проводили до і після курсу сенсорної депривації. Результати. У клінічній картині переважала стволова симптоматика, порушення діяльності ВНС, лімбіко-ретикулярного комплексу з гальмуванням рефлекторної сфери, астенізація, порушення циркадних ритмів, порушення емоційно-вольової сфери, наявність гіпоталамо-стовбурових пароксизмів. Висновки. Сенсорна депривація впливає на функціональний стан надсегментарних структур ВНС та на різні показники у хворих із наслідками бойової ЧМТ, тобто сприяє відновленню збалансованості та синхронності у діяльності надсегментарних структур.Relevance. Traumatic brain injury (TBI) and its consequences often lead to disability, the progression of both the syndromes that arise after trauma and comorbid pathology. Resistance to emerging therapy dictates the search for new pathogenetically grounded, effective approaches to the treatment of this pathology. Particular attention should be paid to the consequences of mild brain lesions, the number of which is increasing, and their diagnosis is difficult, incl. due to compensation in the early stages of low states, ignoring the existing symptoms, followed by inadequacy of therapeutic measures, which leads to persistent violations of the mechanisms of regulation and adaptation. Objective of the study was to study sensory deprivation on the dynamics of indices of the autonomic nervous system (ANS), its neurohormonal link and melatonin hormone in patients with the consequences of mild combat TBI. Materials and methods. We examined 53 patients with the consequences of mild mine-explosive TBI, who were treated with the method of sensory deprivation in a special float chamber. The course of treatment was 10-12 sessions, 45-60 minutes a day. Investigated – neurological status, the state of the ANS: autonomic tone, reactivity, provision of activity, its neurohormonal link (adrenaline, norepinephrine, melatonin), microcirculation in the vessels of the bulbar conjunctiva. A similar examination was carried out after the course of sensory deprivation. Results. The clinical picture was dominated by stem symptoms, impaired activity of the ANS, limbic-reticular complex with inhibition of the reflex sphere, asthenization, disturbances in circadian rhythms, impaired emotional-volitional sphere, the presence of hypothalamic-stem paroxysms. Conclusions. Sensory deprivation has a significant effect on the functional state of the suprasegmental structures of the ANS and other above-mentioned indicators in patients with the consequences of combat TBI, that is, it helps to restore balance and synchronicity in the activity of the suprasegmental structures

Паралоги и порядок дупликации генов алкогольдегидрогеназы человека

By phylogenetic analysis of nucleotide sequences the order of duplication of homologues of human alcohol dehydrogenase genes ADH1A, ADH1B, ADH1C, ADH4, ADH5, ADH6 and ADH7 was determined by Maximum Likelihood method. A dendrogram was constructed according to result of conducted phylogenetic analysis. Evaluation of the evolutionary divergence between sequences of the studied genes was made.Путем филогенетического анализа нуклеотидных последовательностей с помощью метода максимального правдоподобия определен порядок дупликации гомологов генов алкогольдегидрогеназы человека — ADH1A, ADH1B, ADH1C, ADH4, ADH5, ADH6 и ADH7. По результатам филогенетического анализа построена дендрограмма. Проведена оценка эволюционной дивергенции между последовательностями исследуемых генов

Молекулярно-генетичні характеристики аденовірусів, як збудників гострих діарей у жителів півдня України

The etiological role of adenoviruses as causative agents of acute diarrhea in the southern region of Ukraine was studied. Adenovirus DNA was detected in 10.61±1.05 % of clinical stool samples. Serotypes of two gene groups: C and F were identified during genotyping and sequencing of genetic material of detected adenovirus isolates. Gene group F (HAdV41), proves their role in the occurrence of this infectious pathology. HAdV41 isolates had a significant advantage 75.0±15.31 % and had a pronounced genetic heterogeneity, showed similarities with serotypes circulating in previous years in different regions of the world: Sweden (1978), Japan (2003, 2006), South Africa (2009–2014), Iraq (2016). The obtained results allowed to establish the geographical origin of circulating adenoviruses, as well as to predict a further increase in the intensity of the epidemic process of adenoviral infections in southern Ukraine.Вивчали етіологічну роль аденовірусів, як збудників гострі діареї у жителів південного регіону України. ДНК аденовірусів виявлені в клінічних зразках стільця хворих у 10,61±1,05 % випадків. При генотипуванні та секвенуванні генетичного матеріалу виявлених ізолятів ідентифіковано серотипи двох геногруп: С та F. У 25,0±11,69 % хворих на гостру діарею у вигляді моноінфекції було виявлено аденовіруси геногрупи С: HAdV2 і HadV5, що поряд з серотипами геногрупи F (HAdV41), доводить їх роль у виникненні даної інфекційної патології. Ізоляти HAdV41 зустрічались із суттєвою перевагою 75,0±15,31 % та мали виражену генетичну гетерогенність, виявляючи подібність з серотипами, що циркулювали у попередні роки у різних регіонах світу: в Швеції (1978 р.), Японії (2003, 2006 рр.), Південній Африці (2009–2014 рр.), в Іраку (2016 р.). Отримані результати дозволили встановити географічне походження циркулюючих аденовірусів, прогнозувати подальше зростання інтенсивності епідемічного процесу аденовірусної інфекцій на півдні України

Value of conditionally-pathogenic microflora index as predicting factor of bacterial dysbiosis’ development

An article shows the most informative indicators that objectively reflect the condition of the pathological process of dysbiosis, and a system for predicting of the risk of occurrence and severity of dysbiosis according to these indicators is developed. Results of the study substantiated the assumptions about the pathogenetic role of disregulation of hormonal and immune systems in the occurrence of dysbiosis and bacterial vaginosis. Synshronously, the stage of the development of the immune system reaction is discovered in the during the flow of vaginal dysbiosis - from the control stay while normocoenosis to the immunoresistance in grade 1 dysbiosis and expressed combined immunodeficiency in the presence of specifi

The Cytokine System's Status in Bacterial Dysbiosis and Bacterial Vaginosis

Interrelations of conditionally-pathogenic microflora and vaginal mucosa's APC are realized by forming of proinflammatory and regulatory cytokines which can provoke bacterial dysbiosis progression and bacterial vaginosis development.The aim of the study: to determine cytokine system's status in the blood and vaginal fluid in bacterial dysbiosis and bacterial vaginosis.Material and methods. There were used data from 298 women, divided into groups according to the index of conditionally pathogenic microflora and normobiota index: normocenosis (n=53); grade I bacterial dysbiosis (n=128); and grade II bacterial dysbiosis (n=117). In the last group 83 patients with normobiota index >1 lg GE/sample, with bacterial vaginosis isolated. The posterolateral vaginal paries epithelium scrapings examined with PCR: facultative and obligate anaerobes, myco- and ureplasms and yeast fungi were quantified. Contents of IL in VF and in the serum were studied with enzyme-linked immunosorbent assay. For statistical analysis Statistica 10 soft (StatSoft, Inc., USA) was applied.Results. Blood interleukins' contents increased with progressing of bacterial dysbiosis, and was maximal in bacterial vaginosis: 3,0-6,0 times (p<0,001) more than in normocenosis. Levels of these interleukins were: interleukin-1β > interleukin-6>interleukin-8>tumour necrosis factor-α > interleukin-2; in vaginal fluid: interleukin-6> tumour necrosis factor-α > interleukin-1β>interleukin-8> interleukin2. Content of γ-interferon in blood and vaginal fluid was higher in bacterial dysbiosis, and was less in manifested bacterial dysbiosis and bacterial vaginosis (comparing to normocenosis). Interleukin-4 and interleukin-10 levels were less in the blood and vaginal fluid along to the progressing of bacterial dysbiosis. Transforming growth factor-1β level in the blood was more in bacterial vaginosis only, whereas in vaginal fluid –in bacterial dysbiosis and bacterial vaginosis. Among blood cytokines interleukin-1β levels correlated with index of conditionally-pathogenic microflora: its content more 24,6 pg/ml indicated bacterial dysbiosis-II, from 9,6 to 24,5 pg/ml –bacterial dysbiosis-I, and contents less than 9,6 pg/ml – normocenosis. Transforming growth factor-1β and interleukin-10 contents in vaginal fluid were suppressive.Conclusion. Obtained data confirmed determining role of cytokine system in bacterial dysbiosis progression and bacterial vaginosis development. Content of proinflammatory cytokines in the bloodstream increased with progressing of dysbiosis and reached maximum I bacterial vaginosis. Content of anti-inflammatory cytokines with progressing of dysbiosis decreased both in the bloodstream and vaginal flui