26 research outputs found

    Coulomb Blockade due to Quantum Phase-Slips Illustrated with Devices

    Full text link
    In order to illustrate the emergence of Coulomb blockade from coherent quantum phase-slip processes in thin superconducting wires, we propose and theoretically investigate two elementary setups, or "devices". The setups are derived from Cooper-pair box and Cooper-pair transistor, so we refer to them as QPS-box and QPS-transistor, respectively. We demonstrate that the devices exhibit sensitivity to a charge induced by a gate electrode, this being the main signature of Coulomb blockade. Experimental realization of these devices will unambiguously prove the Coulomb blockade as an effect of coherence of phase-slip processes. We analyze the emergence of discrete charging in the limit strong phase-slips. We have found and investigated six distinct regimes that are realized depending on the relation between three characteristic energy scales: inductive and charging energy, and phase-slip amplitude. For completeness, we include a brief discussion of dual Josephson-junction devices

    Molecular cloning and characterization of a thermostable esterase/lipase produced by a novel Anoxybacillus flavithermus strain

    Get PDF
    A thermophilic strain producing an extracellular esterase/lipase was isolated from a hot spring in Tǎşnad, Romania, and was identified phenotypically and by 16S rDNA sequencing as Anoxybacillus flavithermus (GenBank ID: JQ267733). The gene encoding the putative carboxyl esterase (GenBank ID: JX494348) was cloned by direct PCR amplification from genomic DNA. The protein, consisting of 246 amino acids and having a predicted molecular weight of 28.03 kDa, is encoded by an ORF of 741 bps. Expression was achieved in Escherichia coli and a recombinant protein with esterolytic activity and estimated molecular weight of 25 kDa was recovered and purified from the periplasmic fraction by IMAC. The purified enzyme, most active at 60-65°C and in the near-neutral range (pH 6.5-8), displayed a half-life at 60°C of about 5 h. Est/Lip displayed a relative tolerance to methanol, DMSO, acetonitrile, and low detergent concentrations (SDS, Triton) increased its thermostability. Highest activity was attained with p-nitrophenyl butyrate, but the enzyme was also able to hydrolyze long chain fatty acid esters, as well as triolein. The primary sequence and predicted tridimensional structure of the enzyme are very similar to those of other Anoxybacillus and Geobacillus carboxyl esterases in a distinct, recently described lipase family. Est/Lip was highly enantioselective, with preference for the (S)-enantiomer of substrates

    Quantum synchronization and transresistance quantization in superconducting devices

    Full text link
    We show theoretically the possibility of quantum synchronization of Josephson and Bloch oscillations in a superconducting device. One needs an LCLC oscillator to achieve exponentially small rate of synchronization errors. The synchronization leads to quantization of transresistance similar to that in (Fractional) Quantum Hall Effect

    Coherent quantum phase slip

    Full text link
    A hundred years after discovery of superconductivity, one fundamental prediction of the theory, the coherent quantum phase slip (CQPS), has not been observed. CQPS is a phenomenon exactly dual to the Josephson effect: whilst the latter is a coherent transfer of charges between superconducting contacts, the former is a coherent transfer of vortices or fluxes across a superconducting wire. In contrast to previously reported observations of incoherent phase slip, the CQPS has been only a subject of theoretical study. Its experimental demonstration is made difficult by quasiparticle dissipation due to gapless excitations in nanowires or in vortex cores. This difficulty might be overcome by using certain strongly disordered superconductors in the vicinity of the superconductor-insulator transition (SIT). Here we report the first direct observation of the CQPS in a strongly disordered indium-oxide (InOx) superconducting wire inserted in a loop, which is manifested by the superposition of the quantum states with different number of fluxes. Similarly to the Josephson effect, our observation is expected to lead to novel applications in superconducting electronics and quantum metrology.Comment: 14 pages, 3 figure

    The Circadian Clock Protein Timeless Regulates Phagocytosis of Bacteria in Drosophila

    Get PDF
    Survival of bacterial infection is the result of complex host-pathogen interactions. An often-overlooked aspect of these interactions is the circadian state of the host. Previously, we demonstrated that Drosophila mutants lacking the circadian regulatory proteins Timeless (Tim) and Period (Per) are sensitive to infection by S. pneumoniae. Sensitivity to infection can be mediated either by changes in resistance (control of microbial load) or tolerance (endurance of the pathogenic effects of infection). Here we show that Tim regulates resistance against both S. pneumoniae and S. marcescens. We set out to characterize and identify the underlying mechanism of resistance that is circadian-regulated. Using S. pneumoniae, we found that resistance oscillates daily in adult wild-type flies and that these oscillations are absent in Tim mutants. Drosophila have at least three main resistance mechanisms to kill high levels of bacteria in their hemolymph: melanization, antimicrobial peptides, and phagocytosis. We found that melanization is not circadian-regulated. We further found that basal levels of AMP gene expression exhibit time-of-day oscillations but that these are Tim-independent; moreover, infection-induced AMP gene expression is not circadian-regulated. We then show that phagocytosis is circadian-regulated. Wild-type flies exhibit up-regulated phagocytic activity at night; Tim mutants have normal phagocytic activity during the day but lack this night-time peak. Tim appears to regulate an upstream event in phagocytosis, such as bacterial recognition or activation of phagocytic hemocytes. Interestingly, inhibition of phagocytosis in wild type flies results in survival kinetics similar to Tim mutants after infection with S. pneumoniae. Taken together, these results suggest that loss of circadian oscillation of a specific immune function (phagocytosis) can have significant effects on long-term survival of infection

    CSI-COP framework for engaging citizen scientists

    No full text
    Abstract This document provides the framework for the optimum method of applying ECSA’s (European Citizen Science Association) ten principles of citizen science engagement for quality assurance in ethical and inclusive citizen science engagement in the CSI-COP project for the purposes of investigating the extent of online tracking. Following a dynamic recruitment drive, a diverse community of citizen scientists will gain new knowledge and practical skills through informal education provided in workshops and a MOOC (massive open online course). The expertise gained will provide an impetus to join the CSI-COP project collaborating with researchers investigating compliance of General Data Protection Regulation (GDPR) on websites and in apps. A series of activities, validating the outcomes of CSI-COP’s citizen scientists, will be essential to ensure the accuracy of the exploration and findings of cookies embedded in websites and in mobile device apps
    corecore