Renewable Energy Resources Impact on Clean Electrical Power by developing the North-West England Hydro Resource Model.

This paper describes the development of a sequential decision support system to promote hydroelectric power in North-West England. The system, composed of integrated models, addresses barriers to the installation of hydroelectric power schemes. Information is linked through an economic assessment which identifies different turbine options, assesses their suitability for location and demand; and combines the different types of information in a way that supports decision making. The system is structured into five components: the hydrological resource is modelled using Low Flows 2000, the turbine options are identified from hydrological, environmental and demand requirements; and the consequences of different solutions will be fed into other components so that the environmental impacts and public acceptability can be assessed and valued. A preliminary case study is presented on an old gunpowder works to illustrate how the resource model may be employed. Historical architectural structures, power uptake and educational instruction of hydro power technology are considered

When it Pays to Rush: Interpreting Morphogen Gradients Prior to Steady-State

During development, morphogen gradients precisely determine the position of gene expression boundaries despite the inevitable presence of fluctuations. Recent experiments suggest that some morphogen gradients may be interpreted prior to reaching steady-state. Theoretical work has predicted that such systems will be more robust to embryo-to-embryo fluctuations. By analysing two experimentally motivated models of morphogen gradient formation, we investigate the positional precision of gene expression boundaries determined by pre-steady-state morphogen gradients in the presence of embryo-to-embryo fluctuations, internal biochemical noise and variations in the timing of morphogen measurement. Morphogens that are direct transcription factors are found to be particularly sensitive to internal noise when interpreted prior to steady-state, disadvantaging early measurement, even in the presence of large embryo-to-embryo fluctuations. Morphogens interpreted by cell-surface receptors can be measured prior to steady-state without significant decrease in positional precision provided fluctuations in the timing of measurement are small. Applying our results to experiment, we predict that Bicoid, a transcription factor morphogen in Drosophila, is unlikely to be interpreted prior to reaching steady-state. We also predict that Activin in Xenopus and Nodal in zebrafish, morphogens interpreted by cell-surface receptors, can be decoded in pre-steady-state.Comment: 18 pages, 3 figure

Comprehensive Observations of a Solar Minimum CME with STEREO

We perform the first kinematic analysis of a CME observed by both imaging and in situ instruments on board STEREO, namely the SECCHI, PLASTIC, and IMPACT experiments. Launched on 2008 February 4, the CME is tracked continuously from initiation to 1 AU using the SECCHI imagers on both STEREO spacecraft, and is then detected by the PLASTIC and IMPACT particle and field detectors on board STEREO-B. The CME is also detected in situ by ACE and SOHO/CELIAS at Earth's L1 Lagrangian point. The CME hits STEREO-B, ACE, and SOHO on 2008 February 7, but misses STEREO-A entirely. This event provides a good example of just how different the same event can look when viewed from different perspectives. We also demonstrate many ways in which the comprehensive and continuous coverage of this CME by STEREO improves confidence in our assessment of its kinematic behavior, with potential ramifications for space weather forecasting. The observations provide several lines of evidence in favor of the observable part of the CME being narrow in angular extent, a determination crucial for deciding how best to convert observed CME elongation angles from Sun-center to actual Sun-center distances.Comment: 27 pages, 10 figures, AASTEX v5.2, accepted by Ap

Characterization of low thermal conductivity PAN-based carbon fibers

The microstructure and surface chemistry of eight low thermal conductivity (LTC) PAN-based carbon fibers were determined and compared with PAN-based fibers heat treated to higher temperatures. Based on wide-angle x ray diffraction, the LTC PAN fibers all appear to have a similar turbostratic structure with large 002 d-spacings, small crystallite sizes, and moderate preferred orientation. Limited small-angle x ray scattering (SAXS) results indicate that, with the exception of LTC fibers made by BASF, the LTC fibers do not have well developed pores. Transmission electron microscopy shows that the texture of the two LTC PAN-based fibers studied (Amoco T350/23X and /25X) consists of multiple sets of parallel, wavy, bent layers that interweave with each other forming a complex three dimensional network oriented randomly around the fiber axis. X ray photoelectron spectroscopy (XPS) analysis finds correlations between heat treated temperatures and the surface composition chemistry of the carbon fiber samples

Insolubility Theorems and EPR Argument

I wish to thank in particular Arthur Fine for very perceptive comments on a previous draft of this paper. Many thanks also to Theo Nieuwenhuizen for inspiration, to Max Schlosshauer for correspondence, to two anonymous referees for shrewd observations, and to audiences at Aberdeen, Cagliari and Oxford (in particular to Harvey Brown, Elise Crull, Simon Saunders, Chris Timpson and David Wallace) for stimulating questions. This paper was written during my tenure of a Leverhulme Grant on ‘The Einstein Paradox’: The Debate on Nonlocality and Incompleteness in 1935 (Project Grant nr. F/00 152/AN), and it was revised for publication during my tenure of a Visiting Professorship in the Doctoral School of Philosophy and Epistemology, University of Cagliari (Contract nr. 268/21647).Peer reviewedPostprin

Persistence of a pinch in a pipe

The response of low-dimensional solid objects combines geometry and physics in unusual ways, exemplified in structures of great utility such as a thin-walled tube that is ubiquitous in nature and technology. Here we provide a particularly surprising consequence of this confluence of geometry and physics in tubular structures: the anomalously large persistence of a localized pinch in an elastic pipe whose effect decays very slowly as an oscillatory exponential with a persistence length that diverges as the thickness of the tube vanishes, which we confirm experimentally. The result is more a consequence of geometry than material properties, and is thus equally applicable to carbon nanotubes as it is to oil pipelines.Comment: 6 pages, 3 figure

A Budding-Defective M2 Mutant Exhibits Reduced Membrane Interaction, Insensitivity To Cholesterol, And Perturbed Interdomain Coupling

Influenza A M2 is a membrane-associated protein with a C-terminal amphipathic helix that plays a cholesterol-dependent role in viral budding. An M2 mutant with alanine substitutions in the C-terminal amphipathic helix is deficient in viral scission. With the goal of providing atomic-level understanding of how the wild-type protein functions, we used a multipronged site-directed spin labeling electron paramagnetic resonance spectroscopy (SDSL-EPR) approach to characterize the conformational properties of the alanine mutant. We spin-labeled sites in the transmembrane (TM) domain and the C-terminal amphipathic helix (AH) of wild-type (WT) and mutant M2, and collected information on line shapes, relaxation rates, membrane topology, and distances within the homotetramer in membranes with and without cholesterol. Our results identify marked differences in the conformation and dynamics between the WT and the alanine mutant. Compared to WT, the dominant population of the mutant AH is more dynamic, shallower in the membrane, and has altered quaternary arrangement of the C-terminal domain. While the AH becomes more dynamic, the dominant population of the TM domain of the mutant is immobilized. The presence of cholesterol changes the conformation and dynamics of the WT protein, while the alanine mutant is insensitive to cholesterol. These findings provide new insight into how M2 may facilitate budding. We propose the AH–membrane interaction modulates the arrangement of the TM helices, effectively stabilizing a conformational state that enables M2 to facilitate viral budding. Antagonizing the properties of the AH that enable interdomain coupling within M2 may therefore present a novel strategy for anti-influenza drug design

Modelling Molecular Motors as Folding-Unfolding Cycles

We propose a model for motor proteins based on a hierarchical Hamiltonian that we have previously introduced to describe protein folding. The proposed motor model has high efficiency and is consistent with a linear load-velocity response. The main improvement with respect to previous models is that this description suggests a connection between folding and function of allosteric proteins.Comment: 5 pages RevTeX, 2 Postscript figures, replaced due to LaTeX proble

Microrheology, stress fluctuations and active behavior of living cells

We report the first measurements of the intrinsic strain fluctuations of living cells using a recently-developed tracer correlation technique along with a theoretical framework for interpreting such data in heterogeneous media with non-thermal driving. The fluctuations' spatial and temporal correlations indicate that the cytoskeleton can be treated as a course-grained continuum with power-law rheology, driven by a spatially random stress tensor field. Combined with recent cell rheology results, our data imply that intracellular stress fluctuations have a nearly $1/\omega^2$ power spectrum, as expected for a continuum with a slowly evolving internal prestress.Comment: 4 pages, 2 figures, to appear in Phys. Rev. Let