34 research outputs found
Mercury distribution in different tissues and trophic levels of fish from a tropical reservoir, Brazil
The modelling of important intermetallic phases, existing in Fe-based systems by the combined CALPHAD and ab-initio approach
The modelling of important intermetallic phases, existing in Fe-based systems by the combined CALPHAD and ab-initio approach
Method development for the control determination of mercury in seafood by solid-sampling thermal decomposition amalgamation atomic absorption spectrometry (TDA AAS)
Speciation of mercury in various samples from the micro-ecosystem of East Lake by hollow fiber-liquidâliquidâliquid microextraction-HPLC-ICP-MS
Phase stability and mechanical properties of sigma phase in CoâMo system by first principles calculations
Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration
It is currently challenging to adequately model the growth and migration of glioblastoma using twoâdimensional (2D) in vitro culture systems as they quickly lose the original, patientâspecific identity and heterogeneity. However, with the advent of threeâdimensional (3D) cell cultures and humanâinduced pluripotent stem cell (iPSC)âderived cerebral organoids (COs), studies demonstrate that the glioblastomaâCO (GLICO) coculture model helps to preserve the phenotype of the patientâspecific tissue. Here, we aimed to set up such a model using mature COs and develop a pipeline for subsequent analysis of cocultured glioblastoma. Our data demonstrate that the growth and migration of the glioblastoma cell line within the mature COs are significantly increased in the presence of extracellular matrix proteins, shortening the time needed for glioblastoma to initiate migration. We also describe in detail the method for the visualization and quantification of these migrating cells within the GLICO model. Lastly, we show that this coculture model (and the human brainâlike microenvironment) can significantly transform the gene expression profile of the established U87 glioblastoma cell line into proneural and classical glioblastoma cell types