The importance of thyroid function for female reproduction

Background: Thyroid dysfunction is one of the most common endocrine disorder. Thyroid dysfunction affects the female reproductive system and can be manifested by menstrual irregularities, pregnancy loss and infertility. Unexplained infertility has an incidence of 10 to 15 % worldwide. Aim: The general objective of this thesis was to explore the importance of thyroid function for reproduction Material and method: Serum levels of thyroid stimulating hormone (TSH) were compared in three groups of women in early pregnancy, one high-risk group (n = 88), one low-risk group (n = 511) and a general screening group (n = 699). Serum levels of TSH, free thyroxine (fT4) and thyroid peroxidases antibodies (TPO Ab) in fertile women (n = 67) were compared to women with unexplained infertility (n = 147). By using immunohistochemistry, the protein staining of thyroid hormone receptors (TRα1 and TRβ1), TSH receptor (TSH R), mono carboxylate transporter-8 (MCT8), and type 2 iodothyronine deiodinases (DIO2)] in endometrial biopsies were compared between fertile women (n = 19) and women with unexplained infertility (n = 28). Thyroid related proteins in different part of Fallopian tube during the menstrual cycle in fertile women (n=13) were analyzed. Additionally, embryo development until day 6, in 38 human embryos cultured in standard media with T4 added were compared to development of 36 embryos cultured in standard media. Results: The incidence of subclinical hypothyroidism and hypothyroidism was almost the same in all three study groups (almost 10 %). Hypothyroid women on levothyroxine (LT4) supplementation had in almost 50 % of cases an inadequate treatment. Women with unexplained infertility had significantly higher serum level of fT4, and lower protein staining of TRα1 and MCT8, in the endometrium. Supplementation of thyroid hormone in vitro culture media improved the blastocyst development. Additionally, we showed thyroid related proteins in the Fallopian tube. Conclusion: It can be concluded that a general screening for thyroid dysfunction during early pregnancy, by use of TSH levels, is optimal. Furthermore, the imbalance in the thyroid system in women with unexplained infertility highlights the importance of thyroid hormone for female fertility. The improvement of blastocyst development by adding thyroid hormone in early embryo cultures and the presence of proteins related to thyroid in Fallopian tubes suggest involvement of thyroid hormone in early embryo development

Bioinformatics Tools for RNA-seq Data Analysis

RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. The availability of RNA-seq data encouraged computational biologists to develop algorithms to process the data in a statistically disciplinary manner to generate biologically meaningful results. Clustering viral sequences allows us to characterize the composition and structure of intrahost and interhost viral populations, which play a crucial role in disease progression and epidemic spread. In this research, we propose and validate a new entropy-based method for clustering aligned viral sequences considered as categorical data. The method finds a homogeneous clustering by minimizing information entropy rather than the distance between sequences in the same cluster. Moreover in this research, we present a novel pathway analysis method based on Expectation-Maximization (EM) algorithm to study the enzyme expression and pathway activity using meta-transcriptomic data. We will also discuss our approaches to generating unique gene signatures to understand the role of sensory nerve interference in the anti-melanoma immune response and study the racial disparity in Triple-negative breast cancer. Finally, we present our method to detect the retained introns in RNA-seq data to develop a vaccine against cancer having p53 mutations. In summary, this research provides novel approaches to exploring RNA-seq data and their application to real-world biological research

The kite runner and the problem of racism and ethnicity

Racism is a worldwide matter that is based on the physical characteristics of people's division into different categories on which some people become superior and some inferior. Racism and ethnicity are usually considered as the same concepts while in fact ethnicity is a sub-class of racism. In every nation, there are some ethnic groups with the same origin and similar customs that may or may not be judged equally by the power-handler ethnic groups. An example of such country is Afghanistan which is severely an ethnic country. This study explores the social, cultural, and scientific investigations done on the people's races and ethnical characteristics by using Afghan types as examples. Racism is not the result of scientific observation, but it is due to the human differences that happened between 16th and 19th century when people began differentiating among themselves. This aspect of racism is well expressed by the American sociologist "Feagin". In his view, the natural superiority of some people over others is rejected. The novel The Kite Runner depicts the two major Afghan ethnical populations, Pashtun and Hazara, and their social, cultural, and religious conflicts. Accordingly, this article will try to examine the root of ethnic prejudices and oppression among the Afghan people in the novel and the reasons for their ethnic conducts are explained and analyzed based on the Feagin's denial of human difference. Moreover, by providing some evidence it is proved that the biological difference is just some excuses for the powerful section to gain their egotistic goals

Emotional Framing in the Spreading of False and True Claims

The explosive growth of online misinformation, such as false claims, has affected the social behavior of online users. In order to be persuasive and mislead the audience, false claims are made to trigger emotions in their audience. This paper contributes to understanding how misinformation in social media is shaped by investigating the emotional framing that authors of the claims try to create for their audience. We investigate how, firstly, the existence of emotional framing in the claims depends on the topic and credibility of the claims. Secondly, we explore how emotionally framed content triggers emotional response posts by social media users, and how emotions expressed in claims and corresponding users' response posts affect their sharing behavior on social media. Analysis of four data sets covering different topics (politics, health, Syrian war, and COVID-19) reveals that authors shape their claims depending on the topic area to pass targeted emotions to their audience. By analysing responses to claims, we show that the credibility of the claim influences the distribution of emotions that the claim incites in its audience. Moreover, our analysis shows that emotions expressed in the claims are repeated in the users' responses. Finally, the analysis of users' sharing behavior shows that negative emotional framing such as anger, fear, and sadness of false claims leads to more interaction among users than positive emotions. This analysis also reveals that in the claims that trigger happy responses, true claims result in more sharing compared to false claims

IELTS ACADEMIC READING ACHIEVEMENT: THE CONTRIBUTION OF INFERENCE-MAKING AND EVALUATION OF ARGUMENTS

The pivotal undertaking of education today is to endow individuals with the capacity to be able to think flexibly, reason rationally, and have open minds to be able to evaluate and interpret situations. In line with the studies demonstrating the positive relationship between higher-order thinking skills and academic achievement, this study aimed to particularly examine the impact of the two subcomponents of critical thinking, i.e., inference-making and evaluation of arguments on academic IELTS candidates' reading achievements. To achieve the purpose of the study, one hundred and seven IELTS candidates (from different institutes in Mashhad, a city in north of Iran) were asked to complete two tests of the Persian version of the “Watson-Glaser's Critical Thinking Appraisal” after being administered an IELTS reading comprehension test. The results showed that there is a positive relationship between IETLS reading score and EFL learners' inference-making and evaluation of argument. Subsequent data analyses demonstrated that among the variables, inference making is the more powerful predictor of IELTS reading achievement. In addition, the results revealed that the two mentioned variables can predict about 10 percent of IELTS reading achievement. This study has some implications for educators and administrators to take full advantage of these associations by establishing guiding principles for enhancing IELTS candidates' inference-making and evaluation of arguments.  Article visualizations

Carotid plaque hemorrhage on magnetic resonance imaging strongly predicts recurrent ischemia and stroke

Objective There is a recognized need to improve selection of patients with carotid artery stenosis for carotid endarterectomy (CEA). We assessed the value of magnetic resonance imaging (MRI)-defined carotid plaque hemorrhage (MRIPH) to predict recurrent ipsilateral cerebral ischemic events, and stroke in symptomatic carotid stenosis. Methods One hundred seventy-nine symptomatic patients with ≥50% stenosis were prospectively recruited, underwent carotid MRI, and were clinically followed up until CEA, death, or ischemic event. MRIPH was diagnosed if the plaque signal intensity was >150% that of the adjacent muscle. Event-free survival analysis was done using Kaplan–Meier plots and Cox regression models controlling for known vascular risk factors. We also undertook a meta-analysis of reported data on MRIPH and recurrent events. Results One hundred fourteen patients (63.7%) showed MRIPH, suffering 92% (57 of 62) of all recurrent ipsilateral events and all but 1 (25 of 26) future strokes. Patients without MRIPH had an estimated annual absolute stroke risk of only 0.6%. Cox multivariate regression analysis proved MRIPH as a strong predictor of recurrent ischemic events (hazard ratio [HR] = 12.0, 95% confidence interval [CI] = 4.8–30.1, p < 0.001) and stroke alone (HR = 35.0, 95% CI = 4.7–261.6, p = 0.001). Meta-analysis of published data confirmed this association between MRIPH and recurrent cerebral ischemic events in symptomatic carotid artery stenosis (odds ratio = 12.2, 95% CI = 5.5–27.1, p < 0.00001). Interpretation MRIPH independently and strongly predicts recurrent ipsilateral ischemic events, and stroke alone, in symptomatic ≥50% carotid artery stenosis. The very low stroke risk in patients without MRIPH puts into question current risk–benefit assessment for CEA in this subgroup

Effects of royal jelly on sterile skin cut repair

Introduction: Following injury, inflammatory response occurs and the cells below the dermis begin to increase collagen production, then, the epithelial tissue is regenerated. Royal jelly (RJ) has anti-inflammatory activity hence, the aim of this study was to examine the effect of RJ on the induction of sterile skin incision in Balb/C mice. Methods: In an experimental study 60 female Balb/C mice (8 weeks old) were anaesthetized with ether and a longitudinal para vertebral full thickness incision of 10 mm long was made. The animals were divided into six equal groups. Group 1 was considered as negative control. Group 2 (positive control) was treated topically with Nitrofurazon ointment, group 3 with RJ (200 mg/kg) every day, group 4 with RJ (200 mg/kg) every two days, group 5 with RJ (300 mg/kg) every day and group 6 with RJ (300 mg/kg) every two days. The wound length was measured with vernier capilar every two days up to full healing occurred and compared in different groups. Results: There was significant difference between groups 1 or 2 and other groups (p&lt;0.05). RJ promoted wound healing activity significantly in group 3, 5 compared to negative and positive control groups. There was no significant difference between the uses of 200 mg/kg and 300 mg/kg RJ (p&gt;0.05). Conclusion: The results of this study indicate that daily application of RJ possesses betters wound healing effects than Nitrofurazon and every two days usage of RJ.</p

Quantile Regression in Survival Analysis: Comparing Check-Based Modeling and the Minimum Distance Approach

Introduction: Quantile regression is a valuable alternative for survival data analysis, enabling flexible evaluations of covariate effects on survival outcomes with intuitive interpretations. It offers practical computation and reliability. However, challenges arise when applying quantile regression to censored data, particularly for upper quantiles. The minimum distance approach, utilizing dual-kernel estimation and the inverse cumulative distribution function, shows promise in addressing these challenges, especially with Methods: This study contrasts two methods within the realm of quantile linear regression for survival analysis: check-based modeling and the minimum distance approach. Effectiveness is assessed across various scenarios through comprehensive simulation. Results: The simulation results showed that using the quantile regression model with the minimum distance approach reduces the percentage of root mean square error in parameter estimation compared to the quantile regression models based on the check loss function. Additionally, a larger sample size and reduced censoring percentage led to decreased root mean square error in parameter estimation. Conclusion: The research highlights the benefits of using the minimum distance approach for quantile regression. It reduces errors, improves model predictions, captures patterns, and optimizes parameters even with complete data. However, this approach has limitations. The accuracy of estimated quantiles can be influenced by the choice of distance metric and weighting scheme. The assumption of independence between censoring mechanism and survival time may not hold in real-world scenarios. Additionally, dealing with large datasets can be computationally complex

Magnetic resonance imaging plaque hemorrhage for risk stratification in carotid artery disease with moderate risk under current medical therapy

Background and Purpose—Magnetic resonance imaging (MRI)–defined carotid plaque hemorrhage (MRIPH) can predict recurrent cerebrovascular ischemic events in severe symptomatic carotid stenosis. It is less clear whether MRIPH can improve risk stratification despite optimized medical secondary prevention in those with moderate risk. Methods—One-hundred fifty-one symptomatic patients with 30% to 99% carotid artery stenosis (median age: 77, 60.5% men) clinically deemed to not benefit from endarterectomy were prospectively recruited to undergo MRI and clinical follow-up (mean, 22 months). The clinical carotid artery risk score could be evaluated in 88 patients. MRIPH+ve was defined as plaque intensity >150% that of adjacent muscle. Survival analyses were performed with recurrent infarction (stroke or diffusion-positive cerebral ischemia) as the main end point. Results—Fifty-five participants showed MRIPH+ve; 47 had low, 36 intermediate, and 5 high carotid artery risk scores. Cox regression showed MRIPH as a strong predictor of future infarction (hazard ratio, 5.2; 95% confidence interval, 1.64–16.34; P=0.005, corrected for degree of stenosis), also in the subgroup with 50% to 69% stenosis (hazard ratio, 4.1; 95% confidence interval, 1–16.8; P=0.049). The absolute risk of future infarction was 31.7% at 3 years in MRIPH+ve versus 1.8% in patients without (P<0.002). MRIPH increased cumulative risk difference of future infarction by 47.1% at 3 years in those with intermediate carotid artery risk score (P=0.004). Conclusions—The study confirms MRIPH to be a powerful risk marker in symptomatic carotid stenosis with added value over current risk scores. For patients undergoing current secondary prevention medication with clinically uncertain benefit from recanalization, that is, those with moderate degree stenosis and intermediate carotid artery risk scores, MRIPH offers additional risk stratification

Impairment of sperm efficiency in mice following short-term nano-titanium dioxide exposure: An experimental study

Background: Titanium dioxide nanoparticles (TiO2NPs) are widely used in many compounds. Recent evidence has displayed some cytotoxic effects of TiO2NPs on male reproduction. Objective: The effects of TiO2NP administration on sperm parameters and chromatin and seminiferous histopathology of male mice were investigated. Materials and Methods: In this experimental study, 32 NMRI male mice (35 ± 3 gr, 8-12-week-old) were divided into four groups (n = 8/each): treated groups were fed orally with 2.5 (group I), 5 (group II) and 10 (group III) mg/kg/day TiO2NPs for 40 days and the control group received phosphate buffered saline. Sperm parameters, DNA integrity and chromatin quality were assessed using chromomycin A3, aniline blue, toluidine blue staining and TUNEL. Hematoxylin eosin staining was performed to measure spermatogenic cells and the total diameter of seminiferous tubules. Also, sex hormone and malondyaldehyde levels were measured. Results: Abnormal sperm tails rose in group III (28.87 ± 4.91) in comparison with the control group (12.75 ± 3.95). However, chromomycin A3 staining and TUNEL showed higher levels in group III in comparison with the control group, whereas aniline blue and toluidine blue staining showed no differences. A significantly lower spermatogenesis index and lumen parameters were observed in group III. Leydig cell numbers, cellular diameters and the area of the seminiferous tubules were lower in the treated groups. The testosterone level was also lower in these groups and the percentage of malondyaldehyde in the seminal fluid was higher. Conclusion: Exact mechanisms of TiO2NPs are not clear; however, cytotoxic and genotoxic effects of TiO2NPs may relate to oxidative stress. Given their widespread use, TiO2NPs should be a public health focus of attention. Key words: Titanium dioxide, Spermatogenesis, Histology, Mouse, Chromatin, TUNEL