49 research outputs found
Dispersion as an Important Step in the Candida albicans Biofilm Developmental Cycle
Biofilms are dynamic microbial communities in which transitions between planktonic and sessile modes of growth occur interchangeably in response to different environmental cues. In the last decade, early events associated with C. albicans biofilm formation have received considerable attention. However, very little is known about C. albicans biofilm dispersion or the mechanisms and signals that trigger it. This is important because it is precisely C. albicans cells dispersed from biofilms that are the main culprits associated with candidemia and establishment of disseminated invasive disease, two of the gravest forms of candidiasis. Using a simple flow biofilm model recently developed by our group, we have performed initial investigations into the phenomenon of C. albicans biofilm dispersion, as well as the phenotypic characteristics associated with dispersed cells. Our results indicate that C. albicans biofilm dispersion is dependent on growing conditions, including carbon source and pH of the media used for biofilm development. C. albicans dispersed cells are mostly in the yeast form and display distinct phenotypic properties compared to their planktonic counterparts, including enhanced adherence, filamentation, biofilm formation and, perhaps most importantly, increased pathogenicity in a murine model of hematogenously disseminated candidiasis, thus indicating that dispersed cells are armed with a complete arsenal of “virulence factors” important for seeding and establishing new foci of infection. In addition, utilizing genetically engineered strains of C. albicans (tetO-UME6 and tetO-PES1) we demonstrate that C. albicans biofilm dispersion can be regulated by manipulating levels of expression of these key genes, further supporting the evidence for a strong link between biofilms and morphogenetic conversions at different stages of the C. albicans biofilm developmental cycle. Overall, our results offer novel and important insight into the phenomenon of C. albicans biofilm dispersion, a key part of the biofilm developmental cycle, and provide the basis for its more detailed analysis
From evolutionary computation to the evolution of things
Evolution has provided a source of inspiration for algorithm designers since the birth of computers. The resulting field, evolutionary computation, has been successful in solving engineering tasks ranging in outlook from the molecular to the astronomical. Today, the field is entering a new phase as evolutionary algorithms that take place in hardware are developed, opening up new avenues towards autonomous machines that can adapt to their environment. We discuss how evolutionary computation compares with natural evolution and what its benefits are relative to other computing approaches, and we introduce the emerging area of artificial evolution in physical systems
Identifying variation in resistance to the take-all fungus, Gaeumannomyces graminis var. tritici, between different ancestral and modern wheat species
Background: Ancestral wheat relatives are important sources of genetic diversity for the introduction of novel traits
for the improvement of modern bread wheat. In this study the aim was to assess the susceptibility of 34 accessions
of the diploid wheat Triticum monococcum (A genome) to Gaeumannomyces graminis var. tritici (Ggt), the causal
agent of take-all disease. The second aim was to explore the susceptibility of tetraploid wheat (T. durum) and the B
genome progenitor species Aegilops speltoides to Ggt.
Results: Field trials, conducted over 5 years, identified seven T. monococcum accessions with a good level of
resistance to take-all when exposed to natural inoculum under UK field conditions. All other accessions were highly
susceptible or did not exhibit a consistent phenotype across years. DArT marker genotyping revealed that whole
genome diversity was not closely related to resistance to take-all within T. monococcum, suggesting that multiple
genetic sources of resistance may exist within the species. In contrast the tetraploid wheat cultivars and Ae. speltoides
were all highly susceptible to the disease, including those with known elevated levels of benzoxazinoids.
Conclusions: The diploid wheat species T. monococcum may provide a genetic source of resistance to take-all disease
that could be utilised to improve the performance of T. aestivum in high disease risk situations. This represents an
extremely valuable resource to achieve economic and sustainable genetic control of this root disease
Self-sustainability Challenges of Plants Colonization Strategies in Virtual 3D Environments
International audienceThe Biosphere is a bountiful source of inspiration for the biologically inclined scientist, though one may be seized by the twists and turns of its complexity. Artificial Life emerged from the conundrum of condensing this overwhelming intricacy into a tractable volume of data. To tackle the distant challenge of studying the long-term dynamics of artificial ecosystems, we focused in this work our efforts on plant-plant interactions in a simplified 3D setting. Through an extension of K. Sims' directed graphs, we devised a polyvalent genotype for artificial plants development. These individuals compete and collaborate with one another in a shared plot of earth subjected to dynamically changing environmental conditions. We illustrate and analyze how the use of multi-objective fitnesses generated a panel of diverse morphologies and strategies. Furthermore, we identify two driving forces of the emerge of self-reproduction and investigate their effect on self-sustainability