355 research outputs found

    Role of dorsomedial striatum neuronal ensembles in incubation of methamphetamine craving after voluntary abstinence

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    Abstract We recently developed a rat model of incubation of methamphetamine craving after choice-based voluntary abstinence. Here, we studied the role of dorsolateral striatum (DLS) and dorsomedial striatum (DMS) in this incubation. We trained rats to self-administer palatable food pellets (6 d, 6 h/d) and methamphetamine (12 d, 6 h/d). We then assessed relapse to methamphetamine seeking under extinction conditions after 1 and 21 abstinence days. Between tests, the rats underwent voluntary abstinence (using a discrete choice procedure between methamphetamine and food; 20 trials/d) for 19 d. We used in situ hybridization to measure the colabeling of the activity marker Fos with Drd1 and Drd2 in DMS and DLS after the tests. Based on the in situ hybridization colabeling results, we tested the causal role of DMS D1 and D2 family receptors, and DMS neuronal ensembles in "incubated" methamphetamine seeking, using selective dopamine receptor antagonists (SCH39166 or raclopride) and the Daun02 chemogenetic inactivation procedure, respectively. Methamphetamine seeking was higher after 21 d of voluntary abstinence than after 1 d (incubation of methamphetamine craving). The incubated response was associated with increased Fos expression in DMS but not in DLS; Fos was colabeled with both Drd1 and Drd2 DMS injections of SCH39166 or raclopride selectively decreased methamphetamine seeking after 21 abstinence days. In Fos-lacZ transgenic rats, selective inactivation of relapse test-activated Fos neurons in DMS on abstinence day 18 decreased incubated methamphetamine seeking on day 21. Results demonstrate a role of DMS dopamine D1 and D2 receptors in the incubation of methamphetamine craving after voluntary abstinence and that DMS neuronal ensembles mediate this incubation. SIGNIFICANCE STATEMENT: In human addicts, abstinence is often self-imposed and relapse can be triggered by exposure to drug-associated cues that induce drug craving. We recently developed a rat model of incubation of methamphetamine craving after choice-based voluntary abstinence. Here, we used classical pharmacology, in situ hybridization, immunohistochemistry, and the Daun02 inactivation procedure to demonstrate a critical role of dorsomedial striatum neuronal ensembles in this new form of incubation of drug craving

    Effects of dietary beta-agonist treatment, Vitamin D3 supplementation and electrical stimulation of carcasses on meat quality of feedlot steers

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    In this study, 20 young steers received no beta-adrenergic agonist (C), 100 animals all received zilpaterol hydrochloride, with 1 group only receiving zilpaterol (Z) while the other 4 groups received zilpaterol and vitamin D3 at the following levels and durations before slaughter: 7 million IU Vit D3 /animal/day for 3 days (3D7M); 7 million IU Vit D3/animal/day for 6 days (6D7M); 7 million IU Vit D3/animal/day for six days with 7 days no supplementation (6D7M7N) and 1 million IU Vit D3/animal/day for 9 days (9D1M). Left carcass sides were electrically stimulated (ES) and the right side not electrically stimulated (NES). Samples were aged for 3 or 14 days post mortem. Parameters included Warner Bratzler shear force (WBSF), myofibril filament length (MFL), sarcomere length and calpastatin and calpain enzyme activities. For drip loss and instrumental colour measurements, samples were analysed fresh (1 day post mortem) or vacuum-aged for 14 days post mortem. Both ES-treatment and prolonged aging reduced WBSF (P < 0.001). Treatments 6D7M, 6D7M7N and Z remained significantly tougher than C (P < 0.001), while 3D7M and 9D1M improved WBSF under NES conditions. ES was shown to be more effective at alleviating beta-adrenergic agonist induced toughness than high vitamin D3 supplementation. Aging increased drip loss, lightness, redness and yellowness while ES increased drip loss. In general, Z showed increased drip loss, lighter meat, and reduced redness. Vitamin D3 supplementation could not consistently overcome the adverse effects of zilpaterol hydrochloride in feedlot steers.Thesis (PhD)--University of Pretoria, 2012.Animal and Wildlife Sciencesunrestricte

    Mobile Technology for Empowering Health Workers in Underserved Communities: New Approaches to Facilitate the Elimination of Neglected Tropical Diseases.

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    BACKGROUND As global mobile phone penetration increases, direct health information communication from hard-to-reach communities is becoming commonplace. Mobile health (mHealth) tools that enable disease control programs to benefit from this information, while simultaneously empowering community members to take control of their own health, are vital to the goal of universal health care. OBJECTIVE Our aim was to highlight the development of the Liverpool mHealth Suite (LMS), which has been designed to address this need and improve health services for neglected tropical diseases being targeted for global elimination, such as lymphatic filariasis. METHODS The LMS has two main communication approaches-short message service and mobile phone apps-to facilitate real-time mass drug administration (MDA) coverage, reporting patient numbers, managing stock levels of treatment supplies, and exchanging health information to improve the quality of care of those affected. RESULTS The LMS includes the MeasureSMS-MDA tool to improve drug supplies and MDA coverage rates in real-time (currently being trialed in urban Tanzania); the MeasureSMS-Morbidity tool to map morbidity, including lymphedema and hydrocele cases (initially piloted in rural Malawi and Ghana, then extended to Ethiopia, and scaled up to large urban areas in Bangladesh and Tanzania); the LyMSS-lymphedema management supply system app to improve distribution of treatments (trialed for 6 months in Malawi with positive impacts on health workers and patients); and the HealthFront app to improve education and training (in development with field trials planned). CONCLUSIONS The current success and scale-up of the LMS by many community health workers in rural and urban settings across Africa and Asia highlights the value of this simple and practical suite of tools that empowers local health care workers to contribute to local, national, and global elimination of disease

    Heterocentric Language in Commonly Used Measures of Social Anxiety: Recommended Alternate Wording

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    A number of self-report measures of social anxiety contain language that appears to assume heterosexuality. It is unclear how such items should be answered by individuals who are not exclusively heterosexual, which may lead to inaccurate measurement of symptoms, perpetuation of stigma, and alienation of respondents. More specific wording could improve measurement accuracy for sexual minorities as well as heterosexual respondents. Gender-neutral wording was developed for items containing the phrase “opposite sex” in commonly used self-report measures of social anxiety (Interaction Anxiousness Scale [Leary, 1983], Social Avoidance and Distress Scale [Watson & Friend, 1969], Social Interaction Anxiety Scale [Mattick & Clarke, 1998], and Social Phobia and Anxiety Inventory [Turner, Beidel, Dancu, & Stanley, 1989]). Undergraduate college students (N = 405; mean age = 19.88, SD = 2.05) completed measures containing original and revised items. Overall, results indicated that the alternate-worded items demonstrated equivalent or slightly stronger psychometric properties compared to original items. Select alternate-worded items are recommended for clinical and research use, and directions for future research are recommended

    The Effects of Social Anxiety and State Anxiety on Visual Attention: Testing the Vigilance–Avoidance Hypothesis

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    A growing theoretical and research literature suggests that trait and state social anxiety can predict attentional patterns in the presence of emotional stimuli. The current study adds to this literature by examining the effects of state anxiety on visual attention and testing the vigilance– avoidance hypothesis, using a method of continuous visual attentional assessment. Participants were 91 undergraduate college students with high or low trait fear of negative evaluation (FNE), a core aspect of social anxiety, who were randomly assigned to either a high or low state anxiety condition. Participants engaged in a free view task in which pairs of emotional facial stimuli were presented and eye movements were continuously monitored. Overall, participants with high FNE avoided angry stimuli and participants with high state anxiety attended to positive stimuli. Participants with high state anxiety and high FNE were avoidant of angry faces, whereas participants with low state and low FNE exhibited a bias toward angry faces. The study provided partial support for the vigilance–avoidance hypothesis. The findings add to the mixed results in the literature that suggest that both positive and negative emotional stimuli may be important in understanding the complex attention patterns associated with social anxiety. Clinical implications and suggestions for future research are discussed

    Morphology, morpho-taxometric and molecular characterization of the invasive alien species Caribbean leatherleaf slug Sarasinula plebeia (Gastropoda: Veronicellidae): a first record in southern Philippines

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    Herein, we first report the comprehensive description of the terrestrial slug, Sarasinula plebeia (Gastropoda: Veronicellidae) by employing morphology, morpho-taxometrics and molecular analysis. A rapid survey on terrestrial slug invasive alien species (IAS) was conducted in La Dicha, Malangas, Zamboanga Sibugay, the Philippines. Obtained COI gene sequences shared 100% similarities to S. plebeia from Brazil (JX532107, KM489378), Dominica (KM489500) and Vietnam (KM489367) and further supported using Bayesian analysis thus designated as S. plebeia isolate LDZS. Notably, the first reported S. plebeia in 2013 from Batan island, Batanes, northern Philippines, characterized through COI gene markers (JQ582277, JQ582278, JQ582279) showed 100% sequence similarities to a closely related veronicellid slug, Laevecaulis alte isolates (LC636101, LC636102, LC636103, and LC636104) from Japan. Taken this into account, our S. plebeia LDZS isolated from an agricultural field is the first report in the Philippines with combined diagnostic tools for the taxon

    2016 BA Annual Visual and Critical Studies

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    The first BA Annual publication celebrates the work of current and graduating students in OCAD University’s first Bachelor of Arts (BA) Honours program in Visual and Critical Studies. Comprising critical essays, exhibition reviews, artist portfolios, and creative non-fiction, the inaugural volume reflects the innovative and interdiscipli- nary approaches to art history and visual culture that are at the core of this unique program

    Automatic Thoughts and Cognitive Restructuring in Cognitive Behavioral Group Therapy for Social Anxiety Disorder

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    The goal in (Heimberg, R. G. [1991]. A manual for conducting Cognitive Behavior Group Therapy for social phobia (2nd ed.), unpublished manuscript) cognitive behavioral group therapy (CBGT) for social anxiety disorder (social phobia) is to challenge irrational automatic thoughts and create exposures to provide disconfirming evidence for these irrational thoughts as well as habituation to fearful stimuli. Yet little is known about the types of thoughts reported by socially anxious individuals in therapy or which thoughts therapists select for cognitive restructuring in CBGT sessions. The present study analyzed the semantic content of automatic thoughts reported in CBGT and found that the most common thoughts related to poor social performance, negative labels by others, and the anticipation of negative outcomes in feared situations. Principle components analyses indicated the automatic thoughts reflected three underlying themes: Experiencing Anxiety, Negative Self-Evaluation, and Fear of Negative Evaluation. The paper also describes exploratory analyses of which thoughts became the focus of cognitive restructuring exercises and their relationship to treatment outcome. Implications for cognitive therapy are also discussed
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