THE EFFECTS OF BLOOD FLOW RESTRICTION ON MEASURES OF GROSS MOTOR COORDINATION DURING THE WINGATE ANAEROBIC TEST

To date little research has addressed the impact of blood flow restriction (BFR) training upon gross motor coordination measures (GMCM) during a wide variety of maximal activities. The purpose of this study was to assess the effects of BFR on GMCM exhibited during maximal cycling. The performance of 14 females between the ages of eighteen and thirty-five were analyzed during the Wingate Anaerobic Test (WAnT). The participants completed the test under two conditions, using BFR and without. Results showed statistically significant differences (p ≤ 0.05) between conditions for dependent variables assessed throughout this common 30 second test of maximal cycling. These findings suggest that BFR negatively influenced GMCM exhibited during the WAnT

Mood and Performance Anxiety in High School Basketball Players: A Pilot Study

International Journal of Exercise Science 10(4): 604-618, 2017. Participation in competitive sport may impact psychological measures, such as mood and performance anxiety, which in turn may impact enjoyment, adherence, continued participation, and so on. This study assessed the feasibility – in terms of process, resources, management, and potential scientific value– of measuring the effect of varying competitive challenges upon the mood and performance anxiety measures of high school athletes. The participants (n=12) consisted of the boys’ varsity basketball team at a high school in a rural Midwestern community. Participants completed the Profile of Mood States (POMS) to assess mood and the Sport Anxiety Scale-2 (SAS-2) to assess performance anxiety, respectively. Survey administration occurred at baseline and prior to games designated as non-conference, conference, and state tournament. A-priori feasibility measures were achieved in this prospective design. Significant correlations on the subscale measures were found on the POMS and SAS-2 administered before the four conditions in this study; Chronbach’s alpha ranged from 0.54- 0.94 across conditions for POMS subscales, and Chronbach’s alpha ranged from 0.73-0.97 across all conditions for SAS-2 subscales, respectively. Significant differences were found across conditions in the POMS subscale confusion [F(3,33) = 5.71, p = 0.01] and in the SAS-2 subscale worry [F(3,33) = 6.13, p=0.01]. These preliminary findings suggest that the competitive conditions in this study significantly affected some measures of mood and performance anxiety in high school basketball players. These findings warrant further investigation, as well as suggest coaches could gather such information from their players, ultimately aiding in player development and team performance

BLOOD FLOW RESTRICTION DOES NOT AFFECT ACUTE MEASURES OF POWER AND FATIGUE DURING MAXIMAL CYCLING AMONG WOMEN

While it is known that blood flow restriction (BFR) can positively affect training and rehabilitation progression timelines, the physiological basis of this intervention is not fully understood. The purpose of this study was to determine the short-term impact of BFR upon power and fatigue performance measures during maximal cycling. In this study, maximal cycling was assessed using the Wingate Anaerobic Test (WAnT). Using a counterbalanced design, fourteen female participants completed standardized BFR and non-BFR protocols while completing the WAnT. No statistically-significant differences (p ≤ 0.05) were found between conditions for measures of peak power (PP), low power (LP) or fatigue index (FI). These findings suggest that BFR had no statistically-significant acute effect on these performance measures commonly assessed during the WAnT

Use of a promiscuous, constitutively-active bacterial enhancer-binding protein to define the ?54 (RpoN) regulon of Salmonella Typhimurium LT2

BackgroundSigma54, or RpoN, is an alternative σ factor found widely in eubacteria. A significant complication in analysis of the global σ54 regulon in a bacterium is that the σ54 RNA polymerase holoenzyme requires interaction with an active bacterial enhancer-binding protein (bEBP) to initiate transcription at a σ54-dependent promoter. Many bacteria possess multiple bEBPs, which are activated by diverse environmental stimuli. In this work, we assess the ability of a promiscuous, constitutively-active bEBP—the AAA+ ATPase domain of DctD from Sinorhizobium meliloti—to activate transcription from all σ54-dependent promoters for the characterization of the σ54 regulon of Salmonella Typhimurium LT2.ResultsThe AAA+ ATPase domain of DctD was able to drive transcription from nearly all previously characterized or predicted σ54-dependent promoters in Salmonella under a single condition. These promoters are controlled by a variety of native activators and, under the condition tested, are not transcribed in the absence of the DctD AAA+ ATPase domain. We also identified a novel σ54-dependent promoter upstream of STM2939, a homolog of the cas1 component of a CRISPR system. ChIP-chip analysis revealed at least 70 σ54 binding sites in the chromosome, of which 58% are located within coding sequences. Promoter-lacZ fusions with selected intragenic σ54 binding sites suggest that many of these sites are capable of functioning as σ54-dependent promoters.ConclusionSince the DctD AAA+ ATPase domain proved effective in activating transcription from the diverse σ54-dependent promoters of the S. Typhimurium LT2 σ54 regulon under a single growth condition, this approach is likely to be valuable for examining σ54 regulons in other bacterial species. The S. Typhimurium σ54 regulon included a high number of intragenic σ54 binding sites/promoters, suggesting that σ54 may have multiple regulatory roles beyond the initiation of transcription at the start of an operon

Use of a promiscuous, constitutively-active bacterial enhancer-binding protein to define the ?54 (RpoN) regulon of Salmonella Typhimurium LT2

BackgroundSigma54, or RpoN, is an alternative σ factor found widely in eubacteria. A significant complication in analysis of the global σ54 regulon in a bacterium is that the σ54 RNA polymerase holoenzyme requires interaction with an active bacterial enhancer-binding protein (bEBP) to initiate transcription at a σ54-dependent promoter. Many bacteria possess multiple bEBPs, which are activated by diverse environmental stimuli. In this work, we assess the ability of a promiscuous, constitutively-active bEBP—the AAA+ ATPase domain of DctD from Sinorhizobium meliloti—to activate transcription from all σ54-dependent promoters for the characterization of the σ54 regulon of Salmonella Typhimurium LT2.ResultsThe AAA+ ATPase domain of DctD was able to drive transcription from nearly all previously characterized or predicted σ54-dependent promoters in Salmonella under a single condition. These promoters are controlled by a variety of native activators and, under the condition tested, are not transcribed in the absence of the DctD AAA+ ATPase domain. We also identified a novel σ54-dependent promoter upstream of STM2939, a homolog of the cas1 component of a CRISPR system. ChIP-chip analysis revealed at least 70 σ54 binding sites in the chromosome, of which 58% are located within coding sequences. Promoter-lacZ fusions with selected intragenic σ54 binding sites suggest that many of these sites are capable of functioning as σ54-dependent promoters.ConclusionSince the DctD AAA+ ATPase domain proved effective in activating transcription from the diverse σ54-dependent promoters of the S. Typhimurium LT2 σ54 regulon under a single growth condition, this approach is likely to be valuable for examining σ54 regulons in other bacterial species. The S. Typhimurium σ54 regulon included a high number of intragenic σ54 binding sites/promoters, suggesting that σ54 may have multiple regulatory roles beyond the initiation of transcription at the start of an operon

Use of a promiscuous, constitutively-active bacterial enhancer-binding protein to define the �54 (RpoN) regulon of Salmonella Typhimurium LT2

BACKGROUND: Sigma54, or RpoN, is an alternative σ factor found widely in eubacteria. A significant complication in analysis of the global σ(54) regulon in a bacterium is that the σ(54) RNA polymerase holoenzyme requires interaction with an active bacterial enhancer-binding protein (bEBP) to initiate transcription at a σ(54)-dependent promoter. Many bacteria possess multiple bEBPs, which are activated by diverse environmental stimuli. In this work, we assess the ability of a promiscuous, constitutively-active bEBP—the AAA+ ATPase domain of DctD from Sinorhizobium meliloti—to activate transcription from all σ(54)-dependent promoters for the characterization of the σ(54) regulon of Salmonella Typhimurium LT2. RESULTS: The AAA+ ATPase domain of DctD was able to drive transcription from nearly all previously characterized or predicted σ(54)-dependent promoters in Salmonella under a single condition. These promoters are controlled by a variety of native activators and, under the condition tested, are not transcribed in the absence of the DctD AAA+ ATPase domain. We also identified a novel σ(54)-dependent promoter upstream of STM2939, a homolog of the cas1 component of a CRISPR system. ChIP-chip analysis revealed at least 70 σ(54) binding sites in the chromosome, of which 58% are located within coding sequences. Promoter-lacZ fusions with selected intragenic σ(54) binding sites suggest that many of these sites are capable of functioning as σ(54)-dependent promoters. CONCLUSION: Since the DctD AAA+ ATPase domain proved effective in activating transcription from the diverse σ(54)-dependent promoters of the S. Typhimurium LT2 σ(54) regulon under a single growth condition, this approach is likely to be valuable for examining σ(54) regulons in other bacterial species. The S. Typhimurium σ(54) regulon included a high number of intragenic σ(54) binding sites/promoters, suggesting that σ(54) may have multiple regulatory roles beyond the initiation of transcription at the start of an operon