Zooplankton community structure in the Yellow Sea and East China Sea in autumn

Study on zooplankton spatial distribution is essential for understanding food web dynamics in marine ecosystems and fishery management. Here we elucidated the composition and distribution of large mesozooplankton on the continental shelf of the Yellow Sea and East China Sea, and explored the zooplankton community structure in these water masses. Sixty vertical hauls (bottom or 200 m in deep water to surface) using a ring net (diameter 0.8 m, 505-μm mesh) were exploited in November 2007. The biogeographic patterns of zooplankton communities were investigated using multivariate analysis methods; copepod biodiversity was analyzed using univariate indices. Copepods and protozoans were dominate in the communities. Based on the species composition, we divided the study areas into six station groups. Significant differences in zooplankton assemblages were detected between the Yellow Sea and East China Sea. Species richness was higher in East China Sea groups than those in Yellow Sea, whereas taxonomic distinctness was higher in Yellow Sea than in East China Sea. There was a clear relationship between the species composition and water mass group.O estudo da distribuição espacial do zooplancton é essencial para o entendimento não só da dinâmica das teias tróficas nos ecossistemas marinhos, mas também para o manejo da pesca. Neste trabalho procuramos elucidar a composição e distribuição do mesozooplancton na plataforma continental do Mar Amarelo e do Mar da China Oriental, e explorar a estruturas das comunidades nessas duas massas de água. Sessenta arrastos verticais (do fundo ou de 200m até a superfície) foram realizados em Novembro de 2007, usando uma rede circular com diâmetro de 0,8m e malhagem de 505μm. Os padrões biogeográficos das comunidades do zooplancton foram investigados, utilizando-se métodos de análise multivariada. A biodiversidade de Copepoda foi analisada através de indices univariados. Copéodes e protozoários foram os organismos dominantes nas amostragens. Baseados na composição de espécies, pudemos dividir a área de estudo em seis grupos de estações. Diferenças significantes nas assembléias de zooplancton foram detectadas entre o Mar Amarelo e o Mar da China Oriental. A riqueza de espécies foi mais elevada nesta última área, enquanto a distinção taxonômica foi mais alta no Mar Amarelo. Houve uma clara relação entre composição de espécies e tipo de massa de água

Auricle shaping using 3D printing and autologous diced cartilage.

ObjectiveTo reconstruct the auricle using a porous, hollow, three-dimensional (3D)-printed mold and autologous diced cartilage mixed with platelet-rich plasma (PRP).MethodsMaterialise Magics v20.03 was used to design a 3D, porous, hollow auricle mold. Ten molds were printed by selective laser sintering with polyamide. Cartilage grafts were harvested from one ear of a New Zealand rabbit, and PRP was prepared using 10 mL of auricular blood from the same animal. Ear cartilage was diced into 0.5- to 2.0-mm pieces, weighed, mixed with PRP, and then placed inside the hollow mold. Composite grafts were then implanted into the backs of respective rabbits (n = 10) for 4 months. The shape and composition of the diced cartilage were assessed histologically, and biomechanical testing was used to determine stiffness.ResultsThe 3D-printed auricle molds were 0.6-mm thick and showed connectivity between the internal and external surfaces, with round pores of 0.1 to 0.3 cm. After 4 months, the diced cartilage pieces had fused into an auricular shape with high fidelity to the anthropotomy. The weight of the diced cartilage was 5.157 ± 0.230 g (P > 0.05, compared with preoperative). Histological staining showed high chondrocyte viability and the production of collagen II, glycosaminoglycans, and other cartilaginous matrix components. In unrestricted compression tests, auricle stiffness was 0.158 ± 0.187 N/mm, similar to that in humans.ConclusionAuricle grafts were constructed successfully through packing a 3D-printed, porous, hollow auricle mold with diced cartilage mixed with PRP. The auricle cartilage contained viable chondrocytes, appropriate extracellular matrix components, and good mechanical properties.Levels of evidenceNA. Laryngoscope, 129:2467-2474, 2019

Dose and formulation of azithromycin in mass drug administration studies: a systematic review protocol

Introduction: Azithromycin has been given for tropical infectious diseases such as trachoma and yaws by mass drug administration (MDA). As well as controlling the infectious disease in question, MDA may have a beneficial effect in reducing mortality in young children. However, the dose, formulation, frequency and duration of azithromycin used in certain infectious diseases may vary in different studies, and these differences may have impacts on the effectiveness of azithromycin MDA. Furthermore, whether the dose, formulation, frequency and duration are associated with the effectiveness of azithromycin for reducing child mortality—if indeed this effect can be confirmed—remain unknown. In this study, we will investigate whether different strategies such as different dose, formulation, frequency and duration affect the effectiveness of azithromycin MDA on the prevalence of certain infectious diseases or child mortality.Methods and analysis: A narrative systematic review will be conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement. PubMed, Embase, the Cochrane Central Register of Controlled Trials, Web of Science, ClinicalTrials.gov and WHO International Clinical Trials Registry Platform will be searched. No language restrictions will be applied. All randomised/quasi-controlled trials, observational studies (cross-sectional studies, cohort studies and case–control studies), case series and registered protocols will be considered. Dose, duration, frequency, rounds and formulations of azithromycin used in MDA will be collected and reviewed. The outcomes will be disease prevalence/control in children and child mortality. Data from the individual studies will not be pooled

Layer-Based Data Aggregation and Performance Analysis in Wireless Sensor Networks

Due to the similarity and correlation among sensed data in wireless sensor network, it is an important way to reduce the number of packets transmitted with data aggregation technology so as to prolong the network lifetime. However, data aggregation is still a challenging issue since quality-of-service, such as end-to-end delay, is generally considered as a severe criterion required in many applications. We focus on the minimum-latency data aggregation problem and proposed a new efficient scheme for it. The basic idea is that we first build an aggregation tree by ordering nodes into layers, and then we proposed a scheduling algorithm on the basis of the aggregation tree to determine the transmission time slots for all nodes in the network with collision avoiding. We have proved that the upper bound for data aggregation with our proposed scheme is bounded by (15R+Δ-15) for wireless sensor networks in two-dimensional space. Extensive simulation results have demonstrated that the proposed scheme has better practical performance compared with related works

Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-BSA for the Highly Sensitive Detection of Cancer Biomarker

Abstract A simple, convenient, and highly sensitive bio-interface for graphene field-effect transistors (GFETs) based on multifunctional nano-denatured bovine serum albumin (nano-dBSA) functionalization was developed to target cancer biomarkers. The novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface. The formed nano-dBSA film served as the cross-linker to immobilize monoclonal antibody against carcinoembryonic antigen (anti-CEA mAb) on the graphene channel activated by EDC and Sulfo-NHS. The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing. The improved GFET biosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg mL−1. The electrical detection of the binding of CEA followed the Hill model for ligand–receptor interaction, indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82 × 10−10 M. The multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing, nanomedicine, and drug delivery

Inhibition of ERK-DLP1 signaling and mitochondrial division alleviates mitochondrial dysfunction in Alzheimer's disease cybrid cell

Mitochondrial dysfunction is an early pathological feature of Alzheimer’s disease (AD). The underlying mechanisms and strategies to repair it remain unclear. Here, we demonstrate for the first time the direct consequences and potential mechanisms of mitochondrial functional defects associated with abnormal mitochondrial dynamics in AD. Using cytoplasmic hybrid (cybrid) neurons with incorporated platelet mitochondria from AD and age-matched non-AD human subjects into mitochondrial DNA (mtDNA)-depleted neuronal cells, we observed that AD cybrid cells had significant changes in morphology and function; such changes associate with altered expression and distribution of dynamin-like protein (DLP1) and mitofusin 2 (Mfn2). Treatment with antioxidant protects against AD mitochondria-induced extracellular signal-regulated kinase (ERK) activation and mitochondrial fission-fusion imbalances. Notably, inhibition of ERK activation not only attenuates aberrant mitochondrial morphology and function but also restores the mitochondrial fission and fusion balance. These effects suggest a role of oxidative stress-mediated ERK signal transduction in modulation of mitochondrial fission and fusion events. Further, blockade of the mitochondrial fission protein DLP1 by a genetic manipulation with a dominant negative DLP1 (DLP1K38A), its expression with siRNA-DLP1, or inhibition of mitochondrial division with mdivi-1 attenuates mitochondrial functional defects observed in AD cybrid cells. Our results provide new insights into mitochondrial dysfunction resulting from changes in the ERK-fission/fusion (DLP1) machinery and signaling pathway. The protective effect of mdivi-1 and inhibition of ERK signaling on maintenance of normal mitochondrial structure and function holds promise as a potential novel therapeutic strategy for AD

Inhibition of ERK-DLP1 signaling and mitochondrial division alleviates mitochondrial dysfunction in Alzheimer's disease cybrid cell

Mitochondrial dysfunction is an early pathological feature of Alzheimer’s disease (AD). The underlying mechanisms and strategies to repair it remain unclear. Here, we demonstrate for the first time the direct consequences and potential mechanisms of mitochondrial functional defects associated with abnormal mitochondrial dynamics in AD. Using cytoplasmic hybrid (cybrid) neurons with incorporated platelet mitochondria from AD and age-matched non-AD human subjects into mitochondrial DNA (mtDNA)-depleted neuronal cells, we observed that AD cybrid cells had significant changes in morphology and function; such changes associate with altered expression and distribution of dynamin-like protein (DLP1) and mitofusin 2 (Mfn2). Treatment with antioxidant protects against AD mitochondria-induced extracellular signal-regulated kinase (ERK) activation and mitochondrial fission-fusion imbalances. Notably, inhibition of ERK activation not only attenuates aberrant mitochondrial morphology and function but also restores the mitochondrial fission and fusion balance. These effects suggest a role of oxidative stress-mediated ERK signal transduction in modulation of mitochondrial fission and fusion events. Further, blockade of the mitochondrial fission protein DLP1 by a genetic manipulation with a dominant negative DLP1 (DLP1K38A), its expression with siRNA-DLP1, or inhibition of mitochondrial division with mdivi-1 attenuates mitochondrial functional defects observed in AD cybrid cells. Our results provide new insights into mitochondrial dysfunction resulting from changes in the ERK-fission/fusion (DLP1) machinery and signaling pathway. The protective effect of mdivi-1 and inhibition of ERK signaling on maintenance of normal mitochondrial structure and function holds promise as a potential novel therapeutic strategy for AD

Thermal-Enhanced bri1-301 Instability Reveals a Plasma Membrane Protein Quality Control System in Plants

Brassinosteroids (BRs) are essential phytohormones mainly perceived by a single-pass transmembrane receptor-like protein kinase (RLK), BRASSINOSTEROID INSENSITIVE 1 (BRI1). bri1-5 and bri1-9, two distinct mutants with point mutations in the extracellular domain of BRI1, show weak defective phenotypes. Previous studies indicated that bri1-5 and bri1-9 mutated proteins can be recognized and eliminated via an endoplasmic reticulum quality control (ERQC) mechanism. Most of these two proteins, therefore, cannot reach their destination, plasma membrane. Here, we report our functional characterization of bri1-301, another BRI1 mutant protein with an amino acid substitution in the cytoplasmic kinase domain. bri1-301 is a partially functional BR receptor with significantly decreased protein abundance. Interestingly, protein stability and subcellular localization of bri1-301 are temperature-sensitive. At 22°C, an optimal temperature for indoor Arabidopsis growth, bri1-301 shows a weak defective phenotype. At a lower temperature condition such as 18°C, bri1-301 exhibits subtle morphological defects. At a higher temperature condition such as 28°C, on the other hand, bri1-301 displays an extremely severe phenotype reminiscent to that of a null bri1 mutant due to greatly increased bri1-301 internalization and degradation. Our detailed analyses suggest that bri1-301 stability is controlled by ERQC and plasma membrane quality control (PMQC) systems. Since PMQC has not been well studied in plants, bri1-301 can be used as a model mutant for future genetic dissection of this critical process

Service-Oriented Node Scheduling Scheme for Wireless Sensor Networks Using Markov Random Field Model

Future wireless sensor networks are expected to provide various sensing services and energy efficiency is one of the most important criterions. The node scheduling strategy aims to increase network lifetime by selecting a set of sensor nodes to provide the required sensing services in a periodic manner. In this paper, we are concerned with the service-oriented node scheduling problem to provide multiple sensing services while maximizing the network lifetime. We firstly introduce how to model the data correlation for different services by using Markov Random Field (MRF) model. Secondly, we formulate the service-oriented node scheduling issue into three different problems, namely, the multi-service data denoising problem which aims at minimizing the noise level of sensed data, the representative node selection problem concerning with selecting a number of active nodes while determining the services they provide, and the multi-service node scheduling problem which aims at maximizing the network lifetime. Thirdly, we propose a Multi-service Data Denoising (MDD) algorithm, a novel multi-service Representative node Selection and service Determination (RSD) algorithm, and a novel MRF-based Multi-service Node Scheduling (MMNS) scheme to solve the above three problems respectively. Finally, extensive experiments demonstrate that the proposed scheme efficiently extends the network lifetime.This work is supported by the National Science Foundation of China under Grand No. 61370210 and the Development Foundation of Educational Committee of Fujian Province under Grand No. 2012JA12027.Cheng, H.; Su, Z.; Lloret, J.; Chen, G. (2014). Service-Oriented Node Scheduling Scheme for Wireless Sensor Networks Using Markov Random Field Model. Sensors. 14(11):20940-20962. https://doi.org/10.3390/s141120940S2094020962141