Large mammal telomere length variation across ecoregions

Background Telomere length provides a physiological proxy for accumulated stress in animals. While there is a growing consensus over how telomere dynamics and their patterns are linked to life history variation and individual experience, knowledge on the impact of exposure to different stressors at a large spatial scale on telomere length is still lacking. How exposure to different stressors at a regional scale interacts with individual differences in life history is also poorly understood. To better understand large-scale regional influences, we investigated telomere length variation in moose (Alces alces) distributed across three ecoregions. We analyzed 153 samples of 106 moose representing moose of both sexes and range of ages to measure relative telomere lengths (RTL) in white blood cells. Results We found that average RTL was significantly shorter in a northern (montane) and southern (sarmatic) ecoregion where moose experience chronic stress related to severe summer and winter temperatures as well as high anthropogenic land-use compared to the boreal region. Our study suggests that animals in the northern boreal forests, with relatively homogenous land use, are less disturbed by environmental and anthropogenic stressors. In contrast, animals in areas experiencing a higher rate of anthropogenic and environmental change experience increased stress. Conclusion Although animals can often adapt to predictable stressors, our data suggest that some environmental conditions, even though predictable and ubiquitous, can generate population level differences of long-term stress. By measuring RTL in moose for the first time, we provide valuable insights towards our current understanding of telomere biology in free-ranging wildlife in human-modified ecosystems

Always a price to pay: hibernation at low temperatures comes with a trade-off between energy savings and telomere damage

We experimentally tested the costs of deep torpor at low temperatures by comparing telomere dynamics in two species of rodents hibernating at either 3 or 14°C. Our data show that hibernators kept at the warmer temperature had higher arousal frequencies, but maintained longer telomeres than individuals hibernating at the colder temperature. We suggest that the high-energy demand of frequent arousals is counteracted by a lower temperature differential between torpid and euthermic body temperature and that telomere length is restored during arousals when the body temperature is returned to normothermic values. Taken together, our study shows that hibernation at low body temperatures comes with costs on a cellular level and that hibernators need to actively counterbalance the shortening of telomeres

Telomerase activity in ecological studies: What are its consequences for individual physiology and is there evidence for effects and trade‐offs in wild populations

International audienc

dormice_distances_NB_mast

data to analyse the tendencies of dormice to move in different mast situation

Data from: Edible dormice (Glis glis) avoid areas with a high density of their preferred food plant - the European beech

Background: Numerous species, especially among rodents, are strongly affected by the availability of pulsed resources. The intermittent production of large seed crops in northern hemisphere tree species (e.g., beech Fagus spec.,oak Quercus spec., pine trees Pinus spec.) are prime examples of these resource pulses. Adult edible dormice are highly dependent on high energy seeds to maximize their reproductive output. For juvenile dormice the energy rich food is important to grow and fatten in a very short time period prior to hibernation. While these erratic, often large-scale synchronized mast events provide overabundant seed availability, a total lack of seed production can be observed in so-called mast failure years. We hypothesized that dormice either switch territories between mast and non-mast years, to maximize energy availability or select habitats in which alternative food sources are also available (e.g., fleshy fruits, cones). To analyze the habitat preferences of edible dormice we performed environmental niche factor analyses (ENFA) for 9 years of capture-recapture data. Results: As expected, the animals mainly used areas with high canopy closure and vertical stratification, probably to avoid predation. Surprisingly, we found that dormice avoided areas with high beech tree density,but in contrast preferred areas with a relatively high proportion of coniferous trees. Conifer cones and leaves can be an alternative food source for edible dormice and are less variable in availability. Conclusion: Therefore, we conclude that edible dormice try to avoid areas with large fluctuations in food availability to be able to survive years without mast in their territory

dormice_male_captures_environmental

capture numbers for different environmental conditions male

dormice_female_captures_environmental

capture numbers under different environmental conditions for female

dormice_distances_NB

distance analysis for capture events and moving tendencie

Glucocorticoid data field Glis glis females

field data, Glis glis females, glucocorticoids, animal parameters and environmental factor

Data from: The insensitive dormouse: reproduction skipping is not caused by chronic stress in Glis glis

Entire populations of edible dormice (Glis glis) can skip reproduction in years without mast seeding of deciduous trees (particularly beech or oak seed), because juveniles require high caloric seeds for growth and fattening prior to hibernation. We hypothesized that, in mast failure years, female dormice may be forced to spend larger amounts of time foraging for low-quality food, which should increase their exposure to predators, mainly owls. This may lead to chronic stress, i.e., long-term increased secretion of Glucocorticoids (GC), which can have inhibitory effects on reproductive function in both female and male mammals. We monitored reproduction in free-living female dormice over three years with varying levels of food availability, and performed a supplemental feeding experiment. To measure stress hormone levels, we determined fecal GC metabolite (GCM) concentrations collected during the day, which reflect hormone secretion rates in the previous nocturnal activity phase. We found that year-to-year differences in beech mast significantly affected fecal GCM levels and reproduction. However, contrary to our hypothesis, GCM levels were lowest in a non-mast year without reproduction, and significantly elevated in full-mast and intermediate years, as well as under supplemental feeding. Variation in owl density in our study area had no influence on GCM levels. Consequently, we conclude that down-regulation of gonads and reproduction skipping in mast-failure years in this species is not caused by chronic stress. Thus, in edible dormice, delayed reproduction apparently is profitable in response to the absence of energy-rich food in non-mast years, but not in response to chronic stress