Murphy\u27s Law and the Uncertainty of Electron Probes

The electron microscope has brought over the last fifty years a wealth of information about the structure of solids, from surface topography to the details of atomic arrangements. Like any probe, however, the electron beam is subject to the epistomological constraint that the investigator inevitably perturbs what is being investigated, and from its beginnings questions were raised about the integrity of the ·images generated in the microscope from a specimen which was subjected to such an aggressive probe. A fast electron is about as likely to be scattered inelastically as elastically from a collection of atoms, and the density of energy transfer to these atoms, under conditions where their positions or identities can be established, approaches that in a modest nuclear explosion. It is, indeed, a tribute to the redundancy of atomic bonding in solids that atomic organization is largely maintained during investigation. It was early recognized that biological solids were substantially affected; only recently has it been realized that the integrity of atomic-scale information from inorganic solids as well can be seriously compromised by the investigating electron. This contribution reviews the interaction modes which are relevant to the deterioration of specimens in a fast electron beam, outlines the mechanisms by which these interactions lead to irreversible alterations in structure, and assesses the rates at which these alterations proceed in the several instrumental configurations of the electron microscope represented by the scanning electron microscope (SEM), conventional transmission electron microscope (CTEM), and scanning transmission electron microscope (STEM). Incidences of degradation are illustrated for investigation of several structural classes, and several palliative measures are described

Natural functionally-graded composites in hard-to-soft tissue (bone- tendon) junctions

Composite materials are often functionally engineered to imbue desired mechanical properties in materials for structural applications. Nature has long engaged in such composite engineering of biological organisms, which has evolved in both flora and fauna in response to specific mechanical demands. Incorporation of phenolic compounds (like lignin) in stiffening cell assemblies in plant basts, or of silica in plant leaves to resist chomping insect incursions, are good examples in the plant world. Skeletal bone in vertebrates is the classic example in the animal kingdom, a composite of flexible fibrous polymerized organic protein and platy-crystalline inorganic mineral that results in a mechanically strong, hard, tough tissue. The musculo-skeletal system of vertebrates in fact comprises a variety of both hard and soft tissue types (bone, cartilage, tendon, ligament), generative cell types (osteoblasts, chondrocytes, tenocytes, fibroblasts, all of which can derive from multipotent mesenchymal stem cell precursors), and fibrous connective-tissue proteins (chiefly collagen, types I and II) that are susceptible to varying degrees of mineralization. In the case of bone, mineralization is extensive and forms a bi-continuous composite of mineral (chiefly partially-carbonated hydroxyapatite [Ca10(PO4,CO3)6(OH)2] and precursors) and collagen (a triple a-helix polypeptide) that self-assembles into protein fibrils (mostly type I collagen). Bone continually remodels itself and also re-forms as a consequence of injury or around implanted prostheses (such as knee and hip prostheses). High-resolution analytical TEM reveals [1] a mineralization mechanism which entails initial creation, at the mitochondria of bone-forming cells (osteoblasts), of pre-packaged vesicles that fill with a calcium-phosphate hydrogel and thereafter migrate through the cell wall. The vesicle contents subsequently crystallize [2] in the extra-cellular space with the dissolution of the vesicle containment wall, shortly before self-assembling collagen is expressed from the osteoblasts, providing a “just-in-time” ready source of Ca and P for mineralization of collagen fibrils with close to (though not identical with) the Ca/P ratio of hydroxyapatite found in the mature bone composite. The critical connective junctions between different tissue types in the musculo-skeletal system (bone, cartilage, tendon, muscle, ligament) involve several hard-tissue/soft-tissue interfaces, characterized by gradients in mineralization, cell type, cell morphology, and collagen self-assembly modes. For example, standard procedure for re-attachment of ruptured tendons—by surgically re-locating the tendon proximally to bone—re-establishes the important bone-tendon junction (enthesis) in a period of about one year. The process proceeds through growth, contiguous to the (fully mineralized) bone surface, of a partially-mineralized fibrocartilage layer (comprising collagen, expressed by chondrocyte cells, that self-assembles into principally Type II and Type X collagens). TEM [3] of ovine models shows that mineralization of this cartilaginous layer appears to occur via the identical mechanism established [1,2] for bone mineralization but initiated instead by chondrocyte cells. SEM [3] reveals that the cell-type in the remaining unmineralized cartilage portion gradually morphs into tenocytes, which form more elastic tendon fibers comprising, again, mostly Type I collagen (but also Types III, IV, V and IX self-assembly motifs). The resulting hard-tissue/soft-tissue enthesis junction is thus seen [3] to be a multiply graded interface involving three different cell types, several different collagen self-assembly motifs, and the functional gradation of a composite material paradigm spanning fully-hard tissue (bone) to fully-soft tissue (tendon). [1] S. Boonrungsiman, E. Gentleman, R. Carzaniga, N.D. Evans, D.W. McComb, A. E. Porter and M.M. Stevens, PNAS 109 (2012) 141. [2] V. Benezra, L. W. Hobbs and M. Spector, Biomaterials 23 (2001) 725; A. E. Porter, L. W. Hobbs, V. Benezra and M. Spector, Biomaterials 23 (2001) 921. [3] L. W. Hobbs, H. Wang, W. M. Reese, B. M. Tomerline, T. Y. C. Lim, A. E. Porter, M. Walton and M. J. Cotton, Microscopy & Microanalysis 19 (2013) 182

The D0 Run II Impact Parameter Trigger

Many physics topics to be studied by the D0 experiment during Run II of the Fermilab Tevatron ppbar collider give rise to final states containing b--flavored particles. Examples include Higgs searches, top quark production and decay studies, and full reconstruction of B decays. The sensitivity to such modes has been significantly enhanced by the installation of a silicon based vertex detector as part of the DO detector upgrade for Run II. Interesting events must be identified initially in 100-200 microseconds to be available for later study. This paper describes custom electronics used in the DO trigger system to provide the real--time identification of events having tracks consistent with the decay of b--flavored particles.Comment: To be submitted to Nucl. Instrum. Methods A. 56 pages, 31 figure

The Azimuthal Decorrelation of Jets Widely Separated in Rapidity

This study reports the first measurement of the azimuthal decorrelation between jets with pseudorapidity separation up to five units. The data were accumulated using the D{\O}detector during the 1992--1993 collider run of the Fermilab Tevatron at $\sqrt{s}=$ 1.8 TeV. These results are compared to next--to--leading order (NLO) QCD predictions and to two leading--log approximations (LLA) where the leading--log terms are resummed to all orders in $\alpha_{\scriptscriptstyle S}$. The final state jets as predicted by NLO QCD show less azimuthal decorrelation than the data. The parton showering LLA Monte Carlo {\small HERWIG} describes the data well; an analytical LLA prediction based on BFKL resummation shows more decorrelation than the data.Comment: 6 pages with 4 figures, all uuencoded and gzippe

A search for W bb and W Higgs production in ppbar collisions at sqrt(s)=1.96 TeV

We present a search for W b \bar{b} production in p \bar{p} collisions at sqrt{s}=1.96 TeV in events containing one electron, an imbalance in transverse momentum, and two b-tagged jets. Using 174 pb-1 of integrated luminosity accumulated by the D0 experiment at the Fermilab Tevatron collider, and the standard-model description of such events, we set a 95% C.L. upper limit on W b \bar{b}$production of 6.6 pb for b quarks with transverse momenta p_T^b > 20 GeV and b \bar{b} separation in pseudorapidity-azimuth space Delta R_bb > 0.75. Restricting the search to optimized b \bar{b} mass intervals provides upper limits on$WH$production of 9.0$-$12.2 pb, for Higgs-boson masses of 105$-$135 GeV.Comment: 7 pages, 4 figures, 1 table, submitted to Physical Review Letter

Search for R-parity Violating Supersymmetry in Dimuon and Four-Jets Channel

We present results of a search for R-parity-violating decay of the neutralino chi_1^0, taken to be the Lightest Supersymmetric Particle. It is assumed that this decay proceeds through one of the lepton-number violating couplings lambda-prime_2jk (j=1,2; k=1,2,3). This search is based on 77.5 pb-1 of data, collected by the D0 experiment at the Fermilab Tevatron in ppbar collisions at a center of mass energy of 1.8 TeV in 1992-1995.Comment: 10 pages, 3 figure

Direct Search for Charged Higgs Bosons in Decays of Top Quarks

We present a search for charged Higgs bosons in decays of pair-produced top quarks in pbar p collisions at sqrt(s) = 1.8 TeV using 62.2 pb^-1 of data recorded by the D0 detector at the Fermilab Tevatron collider. No evidence is found for signal, and we exclude at 95% confidence most regions of the (M higgs, tan beta) parameter space where the decay t->H b has a branching fraction greater than 0.36 and B(H -> tau nu) is large.Comment: 11 pages, 4 figures, submitted to Phys. Rev. Let

Search for bottom squarks in pbarp collisions at sqrt(s)=1.8 TeV

We report on a search for bottom squarks produced in pbarp collisions at sqrt(s) = 1.8 TeV using the D0 detector at Fermilab. Bottom squarks are assumed to be produced in pairs and to decay to the lightest supersymmetric particle (LSP) and a b quark with branching fraction of 100%. The LSP is assumed to be the lightest neutralino and stable. We set limits on the production cross section as a function of bottom squark mass and LSP mass.Comment: 5 pages, Latex. submitted 3-12-1999 to PRD - Rapid Communicatio

Probing BFKL Dynamics in the Dijet Cross Section at Large Rapidity Intervals in ppbar Collisions at sqrt{s}=1800 and 630 GeV

Inclusive dijet production at large pseudorapidity intervals (delta_eta) between the two jets has been suggested as a regime for observing BFKL dynamics. We have measured the dijet cross section for large delta_eta in ppbar collisions at sqrt{s}=1800 and 630 GeV using the DO detector. The partonic cross section increases strongly with the size of delta_eta. The observed growth is even stronger than expected on the basis of BFKL resummation in the leading logarithmic approximation. The growth of the partonic cross section can be accommodated with an effective BFKL intercept of a_{BFKL}(20GeV)=1.65+/-0.07.Comment: Published in Physical Review Letter

Search for Squarks and Gluinos in Events Containing Jets and a Large Imbalance in Transverse Energy

Using data corresponding to an integrated luminosity of 79 pb-1, D0 has searched for events containing multiple jets and large missing transverse energy in pbar-p collisions at sqrt(s)=1.8 TeV at the Fermilab Tevatron collider. Observing no significant excess beyond what is expected from the standard model, we set limits on the masses of squarks and gluinos and on the model parameters m_0 and m_1/2, in the framework of the minimal low-energy supergravity models of supersymmetry. For tan(beta) = 2 and A_0 = 0, with mu < 0, we exclude all models with m_squark < 250 GeV/c^2. For models with equal squark and gluino masses, we exclude m < 260 GeV/c^2.Comment: 10 pages, 3 figures, Submitted to PRL, Fixed typo on page bottom of p. 6 (QCD multijet background is 35.4 events