Protein stabilization explains the gag requirement for transformation of lymphoid cells by Abelson murine leukemia virus

The single protein encoded by Abelson murine leukemia virus is a fusion of sequence from the retroviral gag genes with the v-abl sequence. Deletion of most of the gag region from the transforming protein results in a virus capable of transforming fibroblasts but no longer capable of transforming lymphoid cells. Smaller deletions in gag reveal that p15 gag sequences are responsible for this effect, whereas deletion of p12 sequences had no effect on lymphoid transformation. In transformed fibroblasts, p15-deleted and normal proteins had similar activities and subcellular localization. When the p15-deleted genome was introduced into previously transformed lymphoid lines, its protein product exhibited a marked instability. The tyrosine-specific autophosphorylation activity per cell was less than 1/20th that of the nondeleted protein. Although pulse-Ia-beling showed that the p15-deleted protein was synthesized efficiently, immunoblotting demonstrated that its steady-state level was less than 1/10th that of the nondeleted Abelson protein. The specific instability of the p15-deleted protein in lymphoid cells explains the requirement of these sequences for lymphoid but not fibroblast transformation

Larval Host Plants of \u3ci\u3eEnodia anthedon\u3c/i\u3e, \u3ci\u3eSatyrodes appalachia\u3c/i\u3e and \u3ci\u3eS. eurydice\u3c/i\u3e in Vermont, USA

Field observation and captive rearing was used to clarify larval host plant use in Enodia anthedon, Satyrodes appalachia, and S. eurydice in Vermont, USA. In nature S. appalachia larvae were found on Carex lacustris, C. lupulina, C. gracillima, and C. tuckermanii. Enodia anthedon in nature was found to oviposit on grass and on Carex lacustris. Larvae of E. anthedon were found on C. lacustris and C. lupulina. In captivity, all but two E. anthedon larvae chose Carex over grass. Both E. appalachia and E. anthedon larvae thrived on a diet of C. lacustris and C. lupulina in captivity. Early instar captive larvae refused C. sparganioides but late instar captive larvae accepted said plant. Grass may be an acceptable alternate host for Vermont S. eurydice. Miscellaneous observations on early instars are reported

Examining the Feasibility of Implementing a Deconstruction Nonprofit in East St. Louis, IL

Background: According to an environmental justice case study by Kozol (2005), East St. Louis is considered the country\u27s most distressed city. It has suffered from environmental and economic misfortunes for several decades. Many residents of the city have left due to the economic conditions of the city, which resulted in a loss of tax base. According to Hou (2010), the loss of tax base has had a severe impact on the community; the city that once had flourishing parks, streets, and businesses has now become blighted with condemned, abandoned, and foreclosed structures. Poor maintenance and neglect has led to decay of many of the structures within the city. While the local government works diligently to improve the economic conditions of the city, it is fiscally constrained (Hou, 2010). Purpose: The purpose of this study is examine the feasibility of implementing a nonprofit organization in East St. Louis, Illinois with a mission of deconstructing condemned, abandoned, and foreclosed structures in order to assist in the development of the community. This study examines the market conditions of the city as well as potential barriers to entry of a deconstruction nonprofit in East St. Louis. Methods: This qualitative study includes a case study of a local St. Louis-based deconstruction nonprofit organization to analyze a regional market conditions. The study further consists of semi-structured interviews of deconstruction nonprofit leaders throughout the nation to realize day-to-day challenges faced with meeting organizational missions. Local public officials are interviewed as well in order to examine what public policies or local government involvement is in place in the community that may attribute to the success or failure of a deconstruction nonprofit. Results: An analysis of the data gathered in study demonstrates that it would be feasible for a deconstruction nonprofit to exist in East St. Louis, Illinois; however, the mission of the organization would have to be expanded to focus more on employment opportunities and civic engagements. While the organization could still aid in deconstructing condemned, abandoned, and foreclosed structures in order to assist in the development of the community as well as divert materials from landfills, the primary focus would have to be the economic and social benefit provided to the citizens of the city of East St. Louis. While challenges exist with working with the city, they can likely be overcome with steady communication and education regarding the benefits of deconstruction. Conclusions: Replicating the Refab nonprofit model with the support of the city of East St. Louis is the most appropriate way forward. Establishing a used building material retail operations (UBMRO) in East St. Louis aids in instilling confidence in city leaders and residents that the nonprofit is there to aid in the development of the city. In order to be successful the nonprofit would need to work closely with the city of East St. Louis

The 60 GHz IMPATT diode development

The objective is to develop 60 GHz IMPATT diodes suitable for communications applications. The performance goals of the 60 GHz IMPATT is 1W CW output power with a conversion efficiency of 15 percent and 10-year lifetime. The final design of the 60 GHz IMPATT structure evolved from computer simulations performed at the University of Michigan. The initial doping profile, involving a hybrid double-drift (HDD) design, was derived from a drift-diffusion model that used the static velocity-field characteristics for GaAs. Unfortunately, the model did not consider the effects of velocity undershoot and delay of the avalanche process due to energy relaxation. Consequently, the initial devices were oscillating at a much lower frequency than anticipated. With a revised simulation program that included the two effects given above, a second HDD profile was generated and was used as a basis for fabrication efforts. In the area of device fabrication, significant progress was made in epitaxial growth and characterization, wafer processing, and die assembly. The organo-metallic chemical vapor deposition (OMCVD) was used. Starting with a baseline X-Band IMPATT technology, appropriate processing steps were modified to satisfy the device requirements at V-Band. In terms of efficiency and reliability, the device requirements dictate a reduction in its series resistance and thermal resistance values. Qualitatively, researchers were able to reduce the diodes' series resistance by reducing the thickness of the N+ GaAs substrate used in its fabrication

Attainability and Nonattainability Under Anti-Pollution Laws

The central issue with which this paper deals is the effectiveness of alternative air pollution control standards presently in use in the United States. More specifically, an analysis and comparison of effluent air standards versus ambient air standards will be performed. The question of effectiveness will be in the context of how well the alternative pollution control measures achieve society\u27s expectations as goals when the standards are imposed. Society\u27s views are assumed to be reflected through a regional (state) planner. The analysis is performed through the use of optimal control techniques. Initially the effluent air standards model will be examined. Next, the ambient air standard model will be analyzed. Finally, a summary and conclusion section will be presented. In general the results suggest that there is a possibility that the optimal path may explode or fall toward zero. In some cases, finite amounts of pollution may occur as equilibria. In any case, the imposition of either type of pollution constraint will effectively reduce the level of social pollution and in some cases the pollution will naturally fall to zero under the constraint. It is also suggested that selective antipollution laws will not, in general, aid in attempts to clean the air

Effects of Supportive Services in a Methadone Treatment Program

A preliminary investigation of the extent to which supportive services contribute to the effectiveness of a methadone treatment program was conducted

Characterization of Internet Traffic and User Classification: Foundations for the Next Generation of Network Emulation

Rigorous experimentation is emerging as the preferred method for validating the benefits of protocols, especially those affecting quality of service. The emulation of traffic generators provides a compact and efficient method for testing protocols and techniques in a realistic context. Models of user behavior serve as the basis for the trafgen traffic emulator and are subject to refinement as our knowledge expands. The project in this study uses a layered approach to study different Internet applications and protocols including the hypertext transfer protocol, http

Notes on Eastern North American Butterflies

Editor’s Note (Harry Pavulaan). New natural history elements and distribution records of several eastern North American butterflies are reported. While diversity and distribution of butterflies in the eastern United States are commonly believed to be fully known, the findings presented here show that much is yet to be learned of our butterfly fauna. Includes: 1. Pages 1-3 Lethe eurydice and L. appalachia Larvae (Nymphalidae: Satyrinae) Eat Their Shed Cuticle (Exuvia) Soon after Molting in Vermont, USA by David J. Hoag Abstract Larvae of Lethe eurydice and L. appalachia were observed eating their newly shed cuticle (exuvia). Further studies are needed to examine the extent of this unique dietary habit within the Satyrinae. 2. Page 4 Virginia state record of Phyciodes phaon (W. H. Edwards, 1864) (Nymphalidae: Limenitidinae) by Harry Pavulaan Abstract A state record specimen of Phyciodes phaon, originally reported by the author in the Virginia Butterfly Bulletin (Pavulaan, 2000), is illustrated for the first time with a view of location collected. 3. Pages 5-13 Butterflies of the Potomac River Woodlands in Leesburg, Loudoun Co., Virginia: A Depauperate Fauna? by Harry Pavulaan Abstract Butterfly observations made over a 16-year period (2005-2020) in the deciduous woodland habitat along the Potomac River in Leesburg, Virginia reveal a depauperate butterfly fauna in a region otherwise known for its rich butterfly diversity. While the forest canopy contains a high diversity of deciduous tree species, and patches of understory shrubs show limited variety, the herb flora of the forest floor is severely lacking due to uncontrolled deer browsing, poor soil conditions and prolonged annual summer droughts. The butterfly fauna is summarized here. 4. Page 14 Limenitis a. arthemis (White Admiral) in Coastal Southeastern Virginia by Brian Taber Abstract Limenitis a. arthemis is reported from coastal southeastern Virginia for the first time. 5. Pages 15-16 Three Species of the Pearly-Eye Genus Enodia (Hübner, 1819) Observed Together in Colonial National Historical Park, Virginia by Kenneth Lorenzen Abstract Butterflies of the Pearly-eye group of satyrs (genus Enodia) are rarely observed together at the same site at the same time. This report documents an occurrence of Enodia anthedon, E. portlandia, and E. creola together at a location near Jamestown, Virginia, United States. Editor’s note: Enodia is now recognized as a subgenus of Lethe. 6. Pages 17-19 Atlides halesus (Cramer, 1777) (Lycaenidae: Theclinae) and American Mistletoe in Northern Virginia by Harry Pavulaan and Richard D. Ullrich Abstract Atlides halesus halesus (Great Purple Hairstreak) is documented from three sites in western Fairfax County, Virginia, United States. The host American Mistletoe (Phoradendron leucarpum) is widespread in this area, forming dense infestations on trees which certainly supports a resident population of the butterfly as evidenced by several records over a 13-year span