19 research outputs found
Byrsonima Crassa Niedenzu (ik): Antimicrobial Activity And Chemical Study
The methanolic extract of leaves from Byrsonima crassa, a Brazilian medicinal plant, was analyzed by CC and HPLC. Four constituents were isolated and identified as quercetin, methyl gallate, (-)-epigallocatechin gallate and quercetin-3-O-(2″-galloyl)-α-L-arabinopyranoside. The methanolic and hydromethanolic extract, as well as fractions, were evaluated regarding their possible antimicrobial activity using in vitro methods. Results showed that both extracts and fractions exhibited significant antimicrobial activity against all tested strains.2617175Agrawal, P.K., (1989) Carbon 13NMR of Flavonoids, , Amsterdam:ElsevierAlzoreky, N.S., Nakahara, K., Antibacterial activity of extracts from some edible plants commonly consumed in Asia (2003) Int J Food Microbiol, 80, pp. 223-230Amarqiuise, A., Che, C.T., Bejar, E., Malone, M.H., Fong, H.H.S., A New Glycolipid from Byrsonima crassifolia (1994) Planta Med, 60, pp. 85-86Basile, A., Sorbo, S., Giordano, S., Ricciardi, L., Ferrara, S., Montesano, D., Cobianchi, R.C., Ferrara, L., Antibacterial and allelopathic activity of extract from Castanea sativa leaves (2000) Fitoterapia, 71, pp. S110-S116Bauer, A.W., Kirby, M.D.K., Sherries, J.C., Truck, M., Antibiotic susceptibilities testing by standard single disc diffusion method (1966) Am J Clin Pathol, 45, pp. 493-496Bejar, E., Amarquaye, A., Che, C.T., Malone, M.H., Fong, H.H.S., Constituents of Byrsonima crassifolia and their spasmogenic activity (1995) Int J Pharmacog, 33, pp. 25-32Binutu, O.A., Cordell, G.A., Gallic acid derivatives from Mezoneuron benthamianum leaves (2000) Pharm Biol, 38, pp. 284-286Djipa, C.D., Delmee, M., Quetin-Leclercq, J., Antimicrobial activity of bark extracts of Syzygium jambos (L.) Alston (Myrtaceae) (2000) J Ethnopharmacol, 71, pp. 307-313Geiss, F., Heinrich, M., Hunkler, D., Rimpler, H., Heinrich, M., Proanthocyanidins with (+)-epicatechin units from Byrsonima crassifolia bark (1995) Phytochemistry, 39, pp. 635-643Gottlieb, O.R., Henriques Mendes, P., Taveira Magalhães, M., Triterpenoids from Byrsonima verbascifolia (1975) Phytochemistry, 14, pp. 1456-1456Harborne, J.B., (1996) The Flavonoids: Advances in Research since 1986, , New York:Chapman & HallLopez, A., Hudson, J.B., Towers, G.H.N., Antiviral and antimicrobial activities of Colombian medicinal plants (2001) J Ethnopharmacol, 77, pp. 189-196Martínez-Vasquéz, M., González-Esquinca, A.R., Cazares Luna, L., Moreno Gutiérrez, M.N., García-Argáez, A.N., Antimicrobial activity of Byrsonima crassifolia (L.) H.B.K (1999) J Ethnopharmacol, 66, pp. 79-82Mendes, C.C., Cruz, F.G., David, J.M., Nascimento, I.P., David, J.P., Triterpenes esterified with fatty acid and triterpene acids isolated from Byrsonima microphylla (1999) Quím Nova, 22, pp. 185-188Nascimento, G.G.F., Locatelli, J., Freitas, P.C., Silva, G.L., Antibacterial activity of plants extracts and phytochemicals on antibiotic-resistant bacteria (2000) Braz J Microbiol, 31, pp. 247-256(2003) Performance Standards for Antimicrobial Disc Susceptibility Tests, , Approved Standard M2-A7, Pennsylvania:WaynePenna, C., Marino, S., Vivot, E., Cruanes, M.C., Munoz, J.D., Cruanes, J., Ferraro, G., Martino, V., Antimicrobial activity of Argentine plants used in the treatment of infectious diseases. Isolation of active compounds from Sebastiania brasiliensis (2001) J Ethnopharmacol, 77, pp. 37-40Pretorius, J.C., Magama, S., Zietsman, P.C., Purification and identification of antibacterial compounds from Euclea crispa subsp crispa (Ebenaceae) leaves (2003) S Afr J Bot, 69, pp. 579-586Rastrelli, L., De Tommasi, N., Berger, I., Caceres, A., Saravia, A., De Simone, F., Glycolipids from Byrsonima crassifolia (1997) Phytochemistry, 45, pp. 647-650Sannomiya, M., Rodrigues, C.M., Coelho, R.G., Santos, L.C., Hiruma-Lima, C.A., Souza Brito, A.R.M., Vilegas, W., Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK) (2004) J Chromatogr A, 1035, pp. 47-51Sannomiya, M., Fonseca, V.B., Da Silva, M.A., Rocha, L.R.M., Dos Santos, L.C., Souza, H.C.A., Brito, A.R.M., Vilegas, W., Flavonoids and antiulcerogenic activity from Byrsonima crassa leaves extracts (2005) J Ethnopharmacol, 97, pp. 1-6Silva, S.R., Silva, A.P., Munhoz, C.B., Silva Jr., M.C., Medeiros, M.B., (2001) Guia de Plantas Do Cerrado Utilizadas Na Chapada Dos Veadeiros, , Brasília:WWF58pSrinivasan, D., Nathan, S., Suresh, T., Perumalsamy, P.L., Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine (2001) J Ethnopharmacol, 74, pp. 217-220Wagner, H., Bladt, H., Zgainski, E.M., (1984) Plant Drug Analysis, , Berlin:Springer320
Cissus Sicyoides: Pharmacological Mechanisms Involved In The Anti-inflammatory And Antidiarrheal Activities
The objective of this study was to evaluate the pharmacological mechanisms involved in anti-inflammatory and antidiarrheal actions of hydroalcoholic extract obtained from the leaves of Cissus sicyoides (HECS). The anti-inflammatory effect was evaluated by oral administration of HECS against acute model of edema induced by xylene, and the mechanisms of action were analysed by involvement of arachidonic acid (AA) and prostaglandin E2 (PGE2). The antidiarrheal effect of HECS was observed and we analyzed the motility and accumulation of intestinal fluid. We also analyzed the antidiarrheal mechanisms of action of HECS by evaluating the role of the opioid receptor, α2 adrenergic receptor, muscarinic receptor, nitric oxide (NO) and PGE2. The oral administration of HECS inhibited the edema induced by xylene and AA and was also able to significantly decrease the levels of PGE2. The extract also exhibited significant anti-diarrheal activity by reducing motility and intestinal fluid accumulation. This extract significantly reduced intestinal transit stimulated by muscarinic agonist and intestinal secretion induced by PGE2. Our data demonstrate that the mechanism of action involved in the anti-inflammatory effect of HECS is related to PGE2. The antidiarrheal effect of this extract may be mediated by inhibition of contraction by acting on the intestinal smoothmuscle and/or intestinal transit. © 2016 by the authors; licensee MDPI, Basel, Switzerland.17
Gastroprotective effect of essential oil from Croton cajucara Benth. (Euphorbiaceae)
Rodriguez, J. Instituto de Biologia, Universidad de Talca, Talca, ChileThe gastroprotective activity of the essential oil from the bark of Croton cajucara Benth (Euphorbiaceae) was assessed in three different models of experimentally induced gastric ulcer in mice. At oral dose of 100 mg/kg the essential oil reduced gastric lesions induced by hypothermic restraint stress and HCl/ethanol significantly. In the HCl/ethanol model a dose-dependent gastroprotective effect was found. Moreover, significant changes in gastric parameters such as pH, secretion rate and total gastric acid were found after intraduodenal administration of essential oil under ligated pylorus (Shay) conditions. The acute toxicity of essential oil was assessed in mice. The LD50 values were 9.3 and 680 mg/kg for oral and intraperitoneal administrations, respectively. The cytotoxicity of essential oil was studied also. A dose-dependent cell viability inhibition was found in V79 fibroblast cell cultures with an IC50 of 22.9 μg/ml. Our results support the pharmacological study of this essential oil
Preliminary studies of Mammea americana L. (Guttiferae) bark/latex extract point to an effective antiulcer effect on gastric ulcer models in mice
Should Anacardium Humile St. Hil Be Used As An Antiulcer Agent? A Scientific Approach To The Traditional Knowledge
The crude methanolic extract (ME) obtained from the leaves of Anacardium humile was evaluated orally at doses of 250-500-1000 mg/kg on gastric lesion on ethanol and piroxicam induced gastric lesions in rodents. All the tested doses significantly inhibited gastric lesions by 56 to 100%. These results seems to support the traditional use of this species in the treatment of gastric diseases. © 2007 Elsevier B.V. All rights reserved.793207209Lorenzi, H., Abreu Matos, F.J., (2002) Plantas medicinais do Brasil: Nativas e Exóticas, , Instituo Plantarum, São PauloMota, M.L., Thomas, G., Barbosa Filho, J.M., (1985) J Ethnopharmacol, 13, p. 289Kubo, I., Ochi, M., Vieira, P.C., Komatsu, S., (1993) J Agr Food Chem, 41, p. 1012Bicalho, B., Pereira, A.S., Aquino Neto, F.R., Pinto, A.C., Rezende, C.M., (2000) J Agr Food Chem, 48, p. 1167Murthy, S.S.N., Anjaneyulu, A.S.R., Ramachandra, R.L., Pelter, A., Ward, R.S., (1982) Planta Med, 45, p. 3Nardini, M., Leonardi, F., Scaccini, C., Virgili, F., (2001) Free Radic Biol Med, 30, p. 722Harbone, J.B., Biflavonoids and triflavonoids (1993) The flavonoids: advance in research since 1986, pp. 95-115. , Greiger H. (Ed), Chapman & Hall, New YorkAgrawal, P.K., Other flavonoids (1989) Carbon 13 NMR of flavonoids, p. 236. , Agrawal P., and Bansal M. (Eds), Elsevier, AmsterdamMorimoto, Y., Shimohara, K., Oshima, S., Sukamoto, T., (1991) Jpn J Pharmacol, 57, p. 495Rainsford, K.D., (1987) J Pharm Pharmacol, 39, p. 669Puscas, I., Puscas, C., Coltau, M., Pasca, R., Torres, J., Marquez, M., (1997) Arzneimittelforschung, 47, p. 568Szelenyi, I., Thiemer, K., (1978) Arch Toxicol, 41, p. 99Brzozowski, T., (2003) J J Physiol Pharmacol, 54, p. 9
Vernonia Polyanthes As A New Source Of Antiulcer Drugs
Methanolic (VPME) and chloroformic (VPCL) extracts, obtained from the aerial parts of Vernonia polyanthes, were investigated for its antiulcerogenic properties. Administration of VPME (250 mg/kg) and VPCL (50 mg/kg) significantly inhibited the gastric mucosa damage (64% and 90%, respectively) caused by absolute ethanol (p.o.). Otherwise, in NSAID-induced gastric damage, their gastroprotective effects have decreased. Since the VPCL extract resulted to be more effective than the VPME we focused our efforts over VPCL action mechanism of action. © 2007 Elsevier B.V. All rights reserved.7807/08/15545551Arrieta, J., Benitez, J., Flores, E., Castillo, C., Navarrete, A., (2003) Planta Med, 69, p. 905Borrelli, F., Izzo, A.A., (2000) Phytother Res, 14, p. 581Rossato, S.C., Master dissertation (1996) Utilização de plantas por populações do litoral norte do estado de São Paulo, , Departamento de Ecologia Geral, Instituto de Biociência, USP, São Paulo, Brazilda Silveira, R.R., Foglio, M.A., Gontijo, J.A.R., (2003) Phytomedicine, 10, p. 127Giordano, O.S., Guerreiro, E., Pestchanker, M.J., Guzman, J., Pastor, D., Guardia, T., (1990) J Nat Prod, 53, p. 803Havsteen, B.H., (2002) Pharmacol Ther, 96, p. 67Bohlmann, F., Jakupovic, J., Gupta, R.K., King, R.M., Robinson, H., (1981) Phytochemistry, 20, p. 473Wagner, H., Bladt, S., (1996) Plant drug analysis a thin layer chromatography atlas, , Springer, BerlinKhan, H.A., (2004) J Pharmacol Toxicol Methods, 49, p. 89Morimoto, Y., Shimohara, K., Oshima, S., Sukamoto, T., (1991) Jpn J Pharmacol, 57, p. 495Rainsford, K.D., (1987) J Pharm Pharmacol, 39, p. 669Shay, H., Komarov, S.A., Fels, S.S., Meranze, D., Gruenstein, M., Siplet, H., (1945) Gastroenterology, 5, p. 43Corne, S.J., Morrissey, S.M., Woods, R.J., (1974) J Physiol, 242. , 116 ppMatsuda, H., Li, Y., Yoshikawa, M., (1999) Life Sci, 65, pp. L27-L32Bandyopadhyay, D., Ghosh, G., Bandyopadhyay, A., Reiter, R.J., (2004) J Pineal Res, 36, p. 195Lewis, D.A., Hanson, P.J., (1991) Prog Med Chem, 28, p. 201Navarrete, A., Trejo-Miranda, J.L., Reyes-Trejo, L., (2002) J Ethnopharmacol, 79, p. 383Cho, C.H., Ogle, C.W., (1990) Dig Dis Sci, 35, p. 1334Haglund, U., (1990) Scand J Gastroenterol, 175, p. 27. , [Suppl]Kwiecien, S., Brzozowski, T., Konturek, S.J., (2002) J Physiol Pharmacol, 53, p. 39Szabo, S., (1987) Scand J Gastroenterol Suppl, 127, p. 21. , [Suppl]Lutnicki, K., Szpringer, E., Czerny, K., Ledwozyw, A., (2001) Folia Morphol (Warsz), 60, p. 47Kaunitz, J.D., Akiba, Y., (2004) Curr Opin Gastroenterol, 20, p. 526Brooks, P., (1998) Am J Med, 104, pp. 9SWallace, J.L., Miller, M.J., (2000) Gastroenterology, 119, p. 512Whittle, B.J., Lopez-Belmonte, J., Moncada, S., (1990) Br J Pharmacol, 99, p. 607Whittle, B.J., Lopez-Belmonte, J., (1993) J Physiol Pharmacol, 44, p. 91Avila, J.R., de la Lastra, C.A., Martin, M.J., Motilva, V., Luque, I., Delgado, D., (1996) Inflamm Res, 45, p. 83Lamberts, R., Creutzfeldt, W., Struber, H.G., Brunner, G., Solcia, E., (1993) Gastroenterology, 104, p. 135
Mechanism and Effect of Esculetin in an Experimental Animal Model of Inflammatory Bowel Disease
Esculetin is a coumarin derivative with high antioxidant activity. In a rat experimental model of inflammatory bowel disease induced by trinitrobenzenesulfonic acid, esculetin at the dose of 5mg/Kg displayed intestinal anti-inflammatory activity; however, its mechanism of action needs to be elucidated. Our objective was to evaluate the effects of esculetin on the intestinal inflammatory process and to clarify the mechanism of action of this compound. We also compared its effects with prednisolone and sulphasalazine. Our results demonstrate that treatment with esculetin prevented an increase in malondialdehyde content, counteracted the depletion of glutathione content, reduced epithelial cell apoptosis, reduced the secretion of pro-inflammatory cytokines, such as IL-1β, IL-2 and IFN-γ, in vitro , and reduced the colonic levels of TNF-α and IL-1β in vivo . Additionally, esculetin treatment inhibited MPO and AP activities. These results demonstrated that esculetin produced a more effective intestinal anti-inflammatory effect than sulphasalazine because it was used at a 10-fold lower dose, and it produced effects similar to those created by prednisolone. We suggest that esculetin exerts its activity by inhibiting pro-inflammatory cytokine secretion and increasing the defences against reactive oxygen species. This leads to less migration and/or activation of inflammatory cells, resulting in the improvement of lesions and functions in the intestinal epithelium. This study confirms the intestinal anti-inflammatory activity of esculetin and demonstrates that this compound has both antioxidative and immunomodulatory properties. Therefore, esculetin may be an interesting new anti-inflammatory drug for the treatment of inflammatory bowel disease
Gastroprotective Effects (in Rodents) Of A Flavonoid Rich Fraction Obtained From Syngonanthus Macrolepsis
Objectives Syngonanthus macrolepis, popularly known in Brazil as 'sempre-vivas', is a plant from the family Eriocaulaceae, it is found in the states of Minas Gerais and Bahia. The species contains a variety of constituents, including flavonoids with gastroprotective effect. In this work, a flavonoid-rich fraction (Sm-FRF) obtained from scapes of S. macrolepis was investigated for preventing gastric ulceration in mice and rats. Methods The activity was evaluated in models of induced gastric ulcer (absolute ethanol, stress, non-steroidal anti-inflammatory drugs and pylorus ligation). The cytoprotective mechanisms of the Sm-FRF in relation to sulfhydryl (SH) groups, nitric oxide (NO) and antioxidant enzymes were also evaluated. Key findings The Sm-FRF (100 mg/kg, p.o.) significantly reduced gastric injury in all models, and did not alter gastric juice parameters after pylorus ligation. Conclusions The results indicate significant gastroprotective activity for the Sm-FRF, which probably involves the participation of both SH groups and the antioxidant system. Both are integral parts of the gastrointestinal mucosa's cytoprotective mechanisms against aggressive factors. © 2013 Royal Pharmaceutical Society.663445452Yeomans, N.D., Naesdal, J., Systematic review: Ulcer definition in NSAID ulcer prevention trials (2008) Aliment Pharmacol Ther, 27, pp. 465-472Martin, G.R., Wallace, J.L., Gastrointestinal inflammation: A central component of mucosal defence and repair (2006) Exp Biol Med, 231, pp. 130-137Tamashiro Filho, P., Evaluation of antiulcer activity and mechanism of action of methanol stem bark extract of Lafoensia pacari A. St.-Hil. (Lytraceae) in experimental animals (2012) J Ethnopharmacol, 144, pp. 497-505Bandyopadhyay, U., Gastroprotective effect of Neem (Azadirachta indica) bark extract: Possible involvement of H(+)-K(+)-ATPase inhibition and scavenging of hydroxyl radical (2002) Life Sci, 71, pp. 2845-2865Malfertheiner, P., Peptic ulcer disease (2009) Lancet, 374, pp. 1449-1461Lin, P.C., The efficacy and safety of proton pump inhibitors vs histamine-2 receptor antagonists for stress ulcer bleeding prophylaxis among critical care patients: A meta-analysis (2010) Crit Care Med, 38, pp. 1197-1205Bansal, V.K., Goel, R.K., Gastroprotective effect of Acacia nilotica young seedless pod extract: Role of polyphenolic constituents (2012) Asian Pac J Trop Med, 5, pp. 523-528Giulietti, A.M., Pirani, J.R., Patterns of geographic distribution of some plant species from the Espinhaço Range, Minas Gerais and Bahia, Brazil (1988) Proceedings of A Workshop on Neotropical Distribution Patterns, pp. 39-69. , Heyer W.R. Vanzolini P.E. eds. Rio de Janeiro: Academia Brasileira de CiênciasGiulietti, A.M., Hensold, N., Padróes de distribuição geográfica dos gêneros de Eriocaulaceae (1990) Acta Bot Brasil, 4, pp. 133-158Lazzari, L.R.P., (2000) Redelimitacão e Revisão de Syngonanthus Sect. Eulepis (Bong. Ex Koern.), , Ruhland - Eriocaulaceae. PhD Thesis, Universidade de São Paulo, BrazilBate-Smith, E.C., Harborne, J.B., Quercetagetin and patuletin in Eriocaulon (1969) Phytochemistry, 8, pp. 1035-1037Vilegas, W., Quercetagetin 7-methyl ether glycosides from Paepalanthus vellozioides and Paepalanthus latipes (1999) Phytochemistry, 51, pp. 403-409Monteiro, M.V., Topical anti-inflammatory, gastroprotective and antioxidant effects of the essential oil of Lippia sidoides Cham. Leaves (2007) J Ethnopharmacol, 111, pp. 378-382Botelho, M.A., Protective effect of locally applied carvacrol gel on ligature-induced periodontitis in rats: A tapping mode AFM study (2009) Phytother Res, 25, pp. 1439-1448Veras, H.N.H., Topical antiinflammatory activity of essential oil of Lippia sidoides cham: Possible mechanism of action (2013) Phytother Res, 27, pp. 179-185Coelho, R.G., Phytochemical study and antiulcerogenic activity of Syngonanthus bisulcatus (Eriocaulaceae) (2006) Braz J Pharm Sci, 42, pp. 413-417Batista, L.M., Gastric antiulcer activity of Syngonanthus arthrotrichus Silveira (2004) Biol Pharm Bull, 27, pp. 328-332Batista, L.M., Gastroprotective effect of the ethanolic extract and fractions obtained from Syngonanthus bisulcatus Rul (2013) Rec Nat Prod, 7, pp. 35-44Agrawal, P.K., (1989) Carbon 13 NMR of Flavonoids, , Amsterdam: Elsevier(1986) Phytochemical Methods, , Harborne J.B. ed. 3rd edn. London: Champman and HallSzelenyi, I., Thiemer, K., Distention ulcer as a model for testing of drugs for ulcerogenic side effects (1978) Arch Toxicol, 41, pp. 99-105Morimoto, Y., Effects of the new anti-ulcer agent KB-5492 on experimental gastric mucosal lesion and gastric mucosal defensive factors, as compared to those of teprenone and cimetidine (1991) Japan J Pharmacol, 57, pp. 495-505Levine, R.J., A method for rapid production of stress ulcers in rats (1971) Peptic Ulcer, pp. 92-97. , Pfeiffer C.J. ed. Copenhagen: MunksgaardRainsford, K.D., Gastric ulcerogenicity of non-steroidal anti-inflamatory drugas in mice with mucosa sensitized by cholinomimetic treatment (1978) Biochem Pharmacol, 27, pp. 1281-1289Shay, H., A simple method for the uniform production of gastric ulceration in the rat (1945) Gastroenterol, 5, pp. 43-61Matsuda, H., Roles of capsaicin-sensitive sensory nerves, endogenous nitric oxide, sulphydryls, and prostaglandins in gastroprotection by mormodin Ic, an oleanolic acid oligoglycoside, on ethanolinduced gastric mucosal lesion in rats (1999) Life Sci, 65, pp. 27-32Sikiric, P., The influence of a novel pentadecapeptide, BPC 157, on NG-nitro-L-arginine methylester and L-arginine effect on stomach mucosa integrity and blood pressure (1997) Eur J Pharmacol, 332, pp. 23-33Yoshikawa, T., Role of active oxygen, lipid, peroxidation, and antioxidants in the pathogenesis of gastric mucosal injury induced by indomethacin in rats (1993) Gut, 34, pp. 732-737Worthington, D.J., Rosemeyer, M.A., Human glutathione reductase: Purification of the crystalline enzyme from erythrocytes (1974) Eur J Biochem, 48, pp. 167-177Winterbourn, C.C., The estimation of red cell superoxide dismutase activity (1975) J Lab Clin Med, 85, pp. 337-341Zapata-Colindres, J.C., The association of Helicobacter pylori infection and nonsteroidal anti-inflammatory drugs in peptic ulcer disease (2006) Can J Gastroenterol, 20, pp. 277-280Lima, G.R.M., Database survey of anti-inflammatory plants in South America: A review (2011) Int J Mol Sci, 12, pp. 2692-2749Lima, G.R.M., Bioactivities of the genus Combretum (Combretaceae): A review (2012) Molecules, 17, pp. 9142-9206Falcão, H.S., Gastric and duodenal antiulcer activity of alkaloids: A review (2008) Molecules, 13, pp. 3198-3223Mota, K.S., Flavonoids with gastroprotective activity (2009) Molecules, 14, pp. 979-1012Jesus, N.Z., Tannins, peptic ulcers and related mechanisms (2012) Int J Mol Sci, 13, pp. 3203-3228Lima, G.R.M., Gastroprotective activity of the ethanolic extract and hexane phase of Combretum duarteanum Cambess. (Combretaceae) (2012) J Nat Med, 67, pp. 492-502Falcão, H.D., Gastroprotective mechanisms of the chloroform and ethyl acetate phases of Praxelis clematidea (Griseb.) R.M.King & H.Robinson (Asteraceae) (2012) J Nat Med, 67, pp. 480-491Sheeba, M.S., Asha, V.V., Effect of Cardiospermum halicacabum on ethanol-induced gastric ulcers in rats (2006) J Ethnopharmacol, 106, pp. 105-110Shaw, S., Effect of ethanol generated free radicals on gastric intrinsic factor and glutathione (1990) Alcohol, 7, pp. 153-157La Casa, C., Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions (2000) J Ethnopharmacol, 71, pp. 45-53Jiang, P., Protective role of metallothionein in stress-induced gastric ulcer in rats (2005) World J Gastroenterol, 11, pp. 2739-2743Konturek, S.J., Healing of chronic gastroduodenal ulcerations by antacids. Role of prostaglandins and epidermal growth factor (1990) Dig Dis Sci, 35, pp. 1121-1129Konturek, P.C., Interaction of Helicobacter pylori (Hp) and non steroidal anti-inflammatory drugs (NSAID) on gastric mucosa and risk of ulcerations (2002) Med Sci Monit, 9, pp. 197-209Toma, W., Modulation of gastrin and epidermal growth factor by pyrrolizidine alkaloids obtained from Senecio brasiliensis in acute and chronic induced gastric ulcers (2004) Can J Physiol Pharmacol, 82, pp. 319-325Bharti, S., Protective effect of Calotropis procera latex extracts on experimentally induced gastric ulcers in rat (2010) J Ethnopharmacol, 127, pp. 440-444Szabo, S., Vattay, P., Experimental gastric and duodenal ulcers. Advances in pathogenesis (1990) Gastroenterol Clin North Am, 19, pp. 67-85Ferreira, M.P., Gastroprotective effect of Cissus sicyoides (Vitaceae): Involvement of microcirculation, endogenous sulfhydryls and nitric oxide (2008) J Ethnopharmacol, 117, pp. 170-174Nagy, L., Protein and non-protein sulfhydryls and disulfides in gastric mucosa and liver after gastrotoxic chemicals and sucralfate: Possible new targets of pharmacologic agents (2007) World J Gastroenterol, 13, pp. 2053-2060Chandranath, S.I., A comparative study on the activity of lansoprazole, omeprazole and PD-136450 on acidified ethanoland indomethacin-induced gastric lesions in the rat (2002) Clin Exp Pharmacol Physiol, 29, pp. 173-180Kanter, M., Gastroprotective activity of Nigella sativa L oil and its constituent, thymoquinone against acute alcohol-induced gastric mucosal injury in rats (2005) World J Gastroenterol, 11, pp. 6662-6666Brzozowski, T., The role of melatonin and L-tryptophan in prevention of acute gastric lesions induced by stress, ethanol, ischemia, and aspirin (1997) J Pineal Res, 23, pp. 79-89El-Missiry, M.A., Protection by metal complexes with SOD-mimetic activity against oxidative gastric injury induced by indomethacin and ethanol in rats (2001) Ann Clin Biochem, 38, pp. 694-700Repetto, M., Protective effect of Artemisia douglasiana Besser extracts in gastric mucosal injury (2003) J Pharm Pharmacol, 55, pp. 551-55
Gastroprotective Mechanisms Of The Chloroform And Ethyl Acetate Phases Of Praxelis Clematidea (griseb.) R.m.king & H.robinson (asteraceae)
Flavonoid-rich Praxelis clematidea (Griseb.) R.M.King & H.Robinson (Asteraceae) is a native plant of South America. This study evaluates the gastroprotective activity and possible mechanisms for both the chloroform (CHCl3P) and ethyl acetate phases (AcOEtP) obtained from aerial parts of the plant. The activity was investigated using acute models of gastric ulcer. Gastric secretion biochemical parameters were determined after pylorus ligature. The participation of cytoprotective factors such as mucus, nitric oxide (NO), sulfhydryl (SH) groups, prostaglandin E2 (PGE 2), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), reduction of lipid peroxidation (malondialdehyde level), and polymorphonuclear infiltration (myeloperoxidase activity), was also investigated. CHCl3P (125, 250, and 500 mg/kg) and AcOEtP (62.5, 125, and 250 mg/kg) showed significant gastroprotective activity, reducing the ulcerative index by 75, 83, 88 % and 66, 66, 81 % for ethanol; 67, 67, 56 % and 56, 53, 58 % for a non-steroidal anti-inflammatory drug (NSAID); and 74, 58, 59 % and 64, 65, 61 % for stress-induced gastric ulcer, respectively. CHCl3P (125 mg/kg) and AcOEtP (62.5 mg/kg) significantly reduced the ulcerative area by 78 and 83 %, respectively, for the ischemia-reperfusion model. They also did not alter the biochemical parameters of gastric secretion, the GSH level or the activities of SOD, GPx or GR. They increased the quantity of gastric mucus, not dependent on NO, yet dependent on SH groups, and maintained PGE2 levels. The P. clematidea phases demonstrated gastroprotective activity related to cytoprotective factors. © 2012 The Japanese Society of Pharmacognosy and Springer.673480491Milani, S., Calabro, A., Role of growth factors and their receptors in gastric ulcer healing (2001) Microsc Res Tech, 53, pp. 360-371. , 11376497 10.1002/jemt.1104 1:CAS:528:DC%2BD3MXks1Kgs7c%3DBandyopadhyay, D., Biswas, K., Bhattacharyya, M., Reiter, R.J., Banerjee, R.K., Gastric toxicity and mucosal ulceration induced by oxygen-derived reactive species: Protection by melatonin (2001) Curr Mol Med, 1, pp. 501-513. , 11899094 10.2174/1566524013363483 1:CAS:528:DC%2BD3MXmsVOgtL8%3DChan, F.K.L., Leung, W.K., Peptic-ulcer disease (2002) Lancet, 360, pp. 933-941. , 12354485 10.1016/S0140-6736(02)11030-0Brozozowski, T., Experimental production of peptic ulcer, gastric damage and cancer models and their use in pathophysiological studies and pharmacological treatment - Polish achievements (2003) J Physiol Pharmacol, 54, pp. 99-126Yuan, Y., Padol, I.T., Hunt, R.H., Peptic ulcer disease today (2006) Nat Clin Pract Gastroenterol Hepatol, 3, pp. 80-89. , 16456574 10.1038/ncpgasthep0393 1:CAS:528:DC%2BD28XhvF2rs78%3DLipof, T., Shapiro, D., Kozol, R.A., Surgical perspectives in peptic ulcer disease and gastritis (2006) World J Gastroenterol, 12, pp. 3248-3252. , 16718847Alper, J.A., Ulcers as an infectious disease (1993) Science, 260, pp. 159-160. , 8469968 10.1126/science.8469968 1:STN:280:DyaK3s3islSitA%3D%3DSandes, A.R.R., Di Blasi, G., Biodiversidade e diversidade química e genética (2000) Biotecnologia Cienc Desenvolv, 13, pp. 28-32Sousa, F.O., (2007) Asteraceae No Parque Estadual da Ilha Do Cardoso, Cananéia, SP, , Thesis to Instituto de Botânica da Secretaria do Meio Ambiente São PauloPollock, S., Holland, A., Smith, W., (2004) New Alien Weed for Queensland Praxelis, , Queensland Herbarium Alert Sheet, Environmental Protection AgencyWang, Z.H., Christie, P., Chen, Q.B., Liu, X.X., Xie, L.L., Bai, C.J., Li, X.L., Allelopathic potential and chemical constituents of volatile oil from Praxelis clematidea (2006) Allelopathy J, 18, pp. 225-235. , 1:CAS:528:DC%2BD2sXhtFGltr%2FKCorlett, R.T., Shaw, J.C., Praxelis clematidea: Yesterday South America, today Hong Kong, tomorrow the world? (1995) Mem Hong Kong Nat Hist Soc, 20, pp. 235-236Noumi, E., Dibakto, T.W., Medicinal plants used for peptic ulcer in the Bangangte region, Western Cameroon (2000) Fitoterapia, 71, pp. 406-412. , 10925012 10.1016/S0367-326X(00)00144-1 1:STN:280:DC%2BD3MzltlSqtw%3D%3DBohlmann, F., Wegner, P., Jakupovic, J., King, R.M., Structure and synthesis of the methyl ester of N-(acetoxy) jasmonoylphenylalanine from Praxelis clematidea (1984) Tetrahedron, 40, pp. 2537-2540. , 10.1016/S0040-4020(01)83506-7 1:CAS:528:DyaL2cXlsVOrs7s%3DMaia, G.L.A., Falcão-Silva, V.S., Aquino, P.G.V., Araújo- Júnior, J.X., Tavares, J.F., Silva, M.S., Rodrigues, L.C., Barbosa-Filho, J.M., Flavonoids from Praxelis clematidea R.M. King and H. Robinson modulate bacterial drug resistance (2011) Molecules, 16, pp. 4828-4835. , 21666549 10.3390/molecules16064828 1:CAS:528:DC%2BC3MXnvVSjsbg%3DAlmeida, J., Silva, M.S., Cunha, E.V.L., Athayde-Filho, P.F., Diniz, M., Silva, M.G., Takemura, O.S., Barbosa-Filho, J.M., Chemical constituents and analgesic activity of Conocliniopsis prasiifolia (2006) Pharm Biol, 44, pp. 76-78. , 10.1080/13880200600582252Agrawal, P.K., (1989) Carbon-13 NMR of Flavonoids, , Elsevier New YorkMiddleton, E.J., Kandaswami, C., Theoharides, T.C., The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer (2000) Pharmacol Rev, 52, pp. 673-751. , 11121513 1:CAS:528:DC%2BD3MXislWktw%3D%3DMorimoto, Y., Shimohara, K., Oshima, S., Sukamoto, T., Effects of the new antiulcer agent KB-5492 on experimental gastric mucosal lesions and gastric mucosal defensive factors, as compared to those of terpenone and cimetidine (1991) Jpn J Pharmacol, 57, pp. 495-505. , 1803065 10.1254/jjp.57.495 1:CAS:528:DyaK38XpvFWhug%3D%3DSzelenyi, I., Thiemer, K., Distention ulcer as a model for testing of drugs for ulcerogenic side effects (1978) Arch Toxicol, 41, pp. 99-105. , 718423 10.1007/BF00351774 1:STN:280:DyaE1M%2FmtFChug%3D%3DPuscas, I., Puscas, C., Coltau, M., Pasça, R., Torres, J., Márquez, M., Herrero, E., Ortiz, J.A., Comparative study of the safety and efficacy of ebrotidine versus ranitidine and placebo in the prevention of piroxicam-induced gastroduodenal lesions (1997) Arzneimittelforschung, 47, pp. 568-572. , 9205767 1:CAS:528:DyaK2sXjvVyqsrY%3DSenay, S.E., Levine, R.J., Synergism between cold and restraint for rapid production of stress ulcer in rats (1967) Proc Soc Exp Biol Med, 124, pp. 1221-1223. , 6024838 1:STN:280:DyaF2s7ktVSjtA%3D%3DUeda, S., Yoshikawa, T., Takahashi, S., Ichikawa, H., Yasuda, M., Oyamada, H., Tanigawa, T., Kondo, M., Role of free radicals and lipid peroxidation in gastric mucosal injury induced by ischemia-reperfusion in rats (1989) Scand J Gastroenterol, 162, pp. 55-58. , 10.3109/00365528909091124 1:STN:280:DyaK3c%2FosVCiug%3D%3DAnderson, M., Determination of glutathione disulfide in biological samples (1985) Methods Enzymmol, 113, pp. 548-555. , 10.1016/S0076-6879(85)13073-9 1:CAS:528:DyaL28XmsFenuw%3D%3DWinterbourn, C.C., Hawkins, R.E., Brian, M., Carrel, R.W., The stimulation of red cell superoxide dismutase activity (1995) J Lab Clin Med, 85, pp. 337-341Yoshikawa, T., Naito, Y., Kishi, A., Tomii, T., Kaneko, T., Iinuma, S., Ichikawa, H., Kondo, M., Role of active oxygen, lipid peroxidation, and antioxidants in the pathogenesis of gastric mucosal injury induced by indomethacin in rats (1993) Gut, 34, pp. 732-737. , 8314503 10.1136/gut.34.6.732 1:STN:280:DyaK3szgtVeqsw%3D%3DCarlberg, I., Mannervik, B., Glutathione reductase (1985) Methods Enzymol, 113, pp. 484-490. , 3003504 10.1016/S0076-6879(85)13062-4 1:CAS:528:DyaL28XkslCqsQ%3D%3DOhkawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal Biochem, 95, pp. 351-358. , 36810 10.1016/0003-2697(79)90738-3 1:CAS:528:DyaE1MXksFaisbk%3DKrawisz, J.E., Sharon, P., Stenson, W.F., Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in the rat and hamster model (1984) Gastroenterology, 87, pp. 1344-1350. , 6092199 1:CAS:528:DyaL2MXktVymShay, H., Komarov, S.A., Fels, S.S., Meranze, D., Gruenstein, M., Siplet, H., A simple method for the uniform production of gastric ulceration in rat (1945) Gastroenterology, 5, pp. 43-61Corne, S.J., Morriessay, S.M., Woods, R.J., A method for the quantitative estimation of gastric barrier mucus (1974) J Physiol, 242, pp. 116-117Curtis, G.H., Macnaughton, W.K., Gall, D.G., Wallace, J.L., Intraluminal pH modulates gastric prostaglandin synthesis (1995) Can J Physiol Pharmacol, 73, pp. 130-134. , 7600443 10.1139/y95-018 1:CAS:528:DyaK2MXkslOmtL8%3DSikiric, P., Seiwerth, S., Grabarevi, E., Ruman, R., Petek, M., Jagi, V., Turkovi, B., Marovi, A., The influence of a novel pentadecapeptide, BPC 157, on NG-nitro-l-arginine methylester and l-arginine effect on stomach mucosa integrity and blood pressure (1997) Eur J Pharmacol, 332, pp. 23-33. , 9298922 10.1016/S0014-2999(97)01033-9Matsuda, H., Li, Y., Yoshikawa, M., Gastroprotections of escins Ia, Ib, IIa, and IIb on ethanol-induced gastric mucosal lesions in rats (1999) Eur J Pharmacol, 373, pp. 63-70. , 10408252 10.1016/S0014-2999(99)00249-6 1:CAS:528:DyaK1MXjvFCitLk%3DHirschowitz, B.I., Keeling, D., Lewin, M., Okabe, S., Parsons, M., Sewing, K., Wallmark, B., Sachs, G., Pharmacological aspects of acid secretion (1995) Dig Dis Sci, 40, pp. 3s-23s. , 7859583 10.1007/BF02214869 1:CAS:528:DyaK2MXkslCqsr8%3DDembieska-Kiec, A., Pallapies, D., Simmet, T., Peskar, B.M., Peskar, B.A., Effect of carbenoxolone on the biological activity of nitric oxide: Relation to gastroprotection (1991) Br J Pharmacol, 104, pp. 811-816. , 10.1111/j.1476-5381.1991.tb12511.xElisabetsky, E., Sociopolitical, economical and ethical issues in medicinal plant research (1991) J Ethnopharmacol, 32, pp. 235-239. , 1881163 10.1016/0378-8741(91)90124-V 1:STN:280:DyaK3MzltVyisw%3D%3DSzabo, S., Trier, J.S., Frankel, P., Sulfhydryl compounds may mediate gastric cytoprotection (1981) Science, 214, pp. 200-202. , 7280691 10.1126/science.7280691 1:CAS:528:DyaL3MXlvVaju7g%3DHirokawa, M., Miura, S., Yoshida, H., Kurose, I., Shigematsu, T., Hokari, R., Higuchi, H., Ishii, H., Oxidative stress and mitochondrial damage precedes gastric mucosal cell death induced by ethanol administration (1998) Alcohol Clin Exp Res, 22, pp. 111S-114S. , 9622385 10.1097/00000374-199803001-00007 1:CAS:528:DyaK1cXjslaju74%3DKvietys, P.R., Twohig, B., Danzell, J., Specian, R.D., Ethanol-induced injury to the rat gastric mucosa. Role of neutrophils and xanthine oxidase-derived radicals (1990) Gastroenterology, 98, pp. 909-920. , 2311875 10.1016/0016-5085(90)90015-S 1:CAS:528:DyaK3cXitFags7c%3DOates, P.J., Hakkinen, J.P., Studies on the mechanism of ethanol-induced gastric damage in rats (1988) Gastroenterology, 94, pp. 10-21. , 3335281 1:CAS:528:DyaL1cXltFSqsA%3D%3DKonturek, S.J., Konturek, P.C., Brzozowski, T., Prostaglandins and ulcer healing (2005) J Physiol Pharmacol, 56, pp. 5-31Dhabhar, F.S., Enhancing versus suppressive effects of stress on immune function: Implications for immunoprotection and immunopathology (2009) Neuroimmunomodulation, 16, pp. 300-317. , 19571591 10.1159/000216188 1:CAS:528:DC%2BD1MXnvFKjtb0%3DDugan, L.L., Choi, D.W., Excitotoxicity, free radicals, and cell membrane changes (1994) Ann Neurol, 35, pp. 17-S21. , 8185290 10.1002/ana.410350707 1:CAS:528:DyaK2cXltVGgsr8%3DPaller, M.S., The cell biology of reperfusion injury in the kidney (1994) J Invest Med, 42, pp. 632-639. , 1:STN:280:DyaK28%2FksF2ksQ%3D%3DValko, M., Rhodes, C.J., Moncol, J., Izakovic, M., Mazur, M., Free radicals, metals and antioxidants in oxidative stress-induced câncer (2006) Chem Biol Interact, 160, pp. 1-40. , 16430879 10.1016/j.cbi.2005.12.009 1:CAS:528:DC%2BD28XhsF2hsrY%3DMojzis, J., Hvisčová, K., Germanová, D., Bukovičová, D., Mirossay, L., Protective effect of quercetin on ischemia/reperfusion induced gastric mucosal injury in rats (2001) Physiol Res, 50, pp. 501-506. , 11702854 1:CAS:528:DC%2BD38Xislensw%3D%3DSchroeter, H., Boyd, C., Spencer, J.P.E., Williams, R.J., Cadenas, E., Rice-Evans, C., MAPK signaling in neurodegeneration: Influences of flavonoids and of nitric oxide (2002) Neurobiology, 23, pp. 861-880. , 1:CAS:528:DC%2BD38XotVChsrw%3DGla, M., Amf, O., Silva Filho, R.N., Vco, C., Tavares, J.F., Assis, T.S., Barbosa Filho, J.M., (2010) Teor de Fenólicos Totais e Capacidade Antioxidante Do Extrato, das Fases e de Flavonóides de Praxelis Clematidea - XXI Simpósio de Plantas Medicinais Do Brasil, , João Pessoa, BrazilDeoda, R.S., Kumar, D., Bhujbal, S.S., Gastroprotective effect of Rubia cordifolia Linn. on aspirin plus pylorus-ligated ulcer (2011) Evid Based Complement Alternat Med, 2011, pp. 541-624. , 10.1155/2011/541624Flemstrom, G., Isenberg, J.I., Gastroduodenal mucosal alkaline secretion and mucosal protection (2001) News Physiol Sci, 16, pp. 23-28. , 11390942 1:CAS:528:DC%2BD3MXjsV2ntbs%3DGrisham, M.B., Von Ritter, C., Smith, B.F., Lamont, J.T., Granger, D.N., Interaction between oxygen radicals and gastric mucin (1987) Am J Physiol, 253, pp. 93-G96. , 3605340 1:CAS:528:DyaL2sXltFSlt74%3DForstner, G., Signal transduction, packaging and secretion of mucins (1995) Annu Rev Physiol, 57, pp. 585-605. , 7778879 10.1146/annurev.ph.57.030195.003101 1:CAS:528:DyaK2MXksVeqsb4%3DAvila, J.R., Lastra, C., Martín, M.J., Motilva, V., Luque, I., Delgado, D., Esteban, J., Herrerias, J., Role of endogenous sulphydryls and neutrophil infiltration in the pathogenesis of gastric mucosal injury induced by piroxicam in rats (1996) Inflamm Res, 45, pp. 83-88. , 8907589 10.1007/BF02265120 1:CAS:528:DyaK28XhslCms7g%3DMotilva, V., López, A., Martin, M.J., La Casa, C., Alacón De La Lastra, C., Cytoprotective activity of cisapride on experimental gastric mucosal lesions induced by ethanol. Role of endogenous prostaglandins (1996) Prostaglandins, 52, pp. 63-74. , 8875638 10.1016/0090-6980(96)00052-4 1:CAS:528:DyaK28XlsVeju7k%3DSalvemini, D., Misko, T.P., Masferrer, J.L., Seibert, K., Currie, M.G., Needleman, P., Nitric oxide activates cyclooxygenase enzymes (1993) Proc Natl Acad Sci USA, 90, pp. 7240-7244. , 7688473 10.1073/pnas.90.15.7240 1:CAS:528:DyaK3sXltlOhu7g%3DKortezova, N.I., Shikova, L.I., Milusheva, E.A., Itzev, D.E., Muscarinic modulation of nitrergic neurotransmission in guinea-pig gastric fundus (2004) Neurogastroenterol Motil, 16, pp. 155-165. , 15086869 10.1111/j.1365-2982.2004.00514.x 1:STN:280: DC%2BD2c7ptFymtw%3D%3DAly, A., The role of nitric oxide and sulfhydryls in gastric mucosal protection induced by sodium cromoglycate in rats (1995) J Pharm Pharmacol, 47, pp. 739-743. , 10.1111/j.2042-7158.1995.tb06734.xSzelenyi, I., Brune, K., Possible role of oxygen free radicals in ethanol-induced gastric mucosal damage in rats (1988) Dig Dis Sci, 33, pp. 865-871. , 3378480 10.1007/BF01550977 1:CAS:528:DyaL1cXks1yksrs%3DRastogi, L., Patnaik, G.K., Dikshit, M., Free radicals and antioxidant status following pylorus ligation induced gastric mucosal injury in rats (1998) Pharmacol Res, 38, pp. 125-132. , 9721600 10.1006/phrs.1998.0343 1:CAS:528:DyaK1cXlsFeiur0%3DBrzozowski, T., Konturek, P.C., Konturek, S.J., Brzozowska, I., Pawlik, T., Role of prostaglandins in gastroprotection and gastric adaptation (2005) J Physiol Pharmacol, 56, pp. 33-55. , 16247188Matsui, H., Shimokawa, O., Kaneko, T., Nagano, Y., Rai, K., Hyodo, I., The pathophysiology of non-steroidal anti-inflammatory drug (NSAID)-induced mucosal injuries in stomach and small intestine (2011) J Clin Biochem Nutr, 48, pp. 107-111. , 21373261 10.3164/jcbn.10-79 1:CAS:528:DC%2BC3MXltlektbY%3
Indigofera Suffruticosa Mill As New Source Of Healing Agent: Involvement Of Prostaglandin And Mucus And Heat Shock Proteins
Ethnopharmacological relevance: Indigofera suffruticosa is specie typical of the "Cerrado" or Brazilian savannah; it is a member of the Fabaceae family - in folkmedicine is used for gastric disorders, infection and inflammation. Aim of the study: Ethyl acetate fraction (AcF) and aqueous fraction (AqF) of the methanolic extract of I. suffruticosa leaves were evaluated against acute gastric ulcer. The AcF fraction was selected to assess its activity in ulcer healing and its gastroprotective effects via mucus and gastric secretion. Materials and methods: The gastroprotective action of AcF and AqF fractions were evaluated in a rodent experimental model. The action mechanisms, involvements of the antisecretory action, mucus and prostaglandin production, toxicological and healing activity of the AcF (100 mg/kg, p.o.) were evaluated. We also used histological analysis (HE and PAS) and immunohistochemical (PCNA and HSP-70) assays to evaluate the effects of I. suffruticosa. Results: AcF significantly inhibited the gastric mucosal damage caused by ethanol. This effect was statistically significant in 100 mg/kg group compared vehicle. AcF did not interfered with gastric secretion, significantly increased the PGE 2 and mucus production (validated in PAS technique). The gastroprotection was attenuated by pretreatment with N-ethylmaleimide, but not L-NAME. In acid-acetic-induced ulcer model AcF accelerated ulcer healing. Immunohistochemistry analysis showed induction of proliferating cell (PCNA) and heat shock protein (HSP 70). Conclusions: These results showed that AcF acted as gastroprotective agent stimulating prostaglandin, mucus and HSP70. © 2011 Elsevier Ireland Ltd All rights reserved.1371192198Arrieta, J., Benitez, J., Flores, E., Castillo, C., Navarrete, A., Purification of Gastroprotective Triterpenoids from the Stem Bark of Amphipterygium adstringensRole of Prostaglandins, Sulfhydryls, Nitric Oxide and Capsaicin-Sensitive Neurons (2003) Planta Medica, 69 (10), pp. 905-909. , DOI 10.1055/s-2003-45098Behmer, O.A., Tolosa, E.M.C., Freitas Neto, A.G., (1976) Manual de Técnicas Para Histologia Normal e Patológica, pp. 80-195. , Editora da USP, São Paulo, SP, BrazilCalvo, T.R., Cardoso, C.R., Da Silva Moura, A.C., Dos Santos, L.C., Colus, I.M., Vilegas, W., Varanda, E.A., Mutagenic activity of Indigofera truxillensis and I. suffruticosa aerial parts (2009) Evidence-Based Complementary and Alternative Medicine, 20, pp. 1-8Cola-Miranda, M., Barbastefano, V., Hiruma-Lima, C.A., Calvo, T.R., Vilegas, W., Souza Brito, A.R.M., Antiulcerogenic activity of Indigofera truxillensis Kunth (2006) Biota Neotropica, 6, pp. 1-9Curtis, G.H., MacNaughton, W.K., Gall, D.G., Wallace, J.L., Intraluminal pH modulates gastric prostaglandin synthesis (1995) Canadian Journal of Physiology and Pharmacology, 73, pp. 130-134De Sousa Falcão, H., Leite, J.A., Barbosa-Filho, J.M., De Athayde-Filho, P.F., De Oliveira Chaves, M.C., Moura, M.D., Ferreira, A.L., Batista, L.M., Gastric and duodenal antiulcer activity of alkaloids: A review (2008) Molecules, 13, pp. 3198-3223Farias-Silva, E., Cola, M., Calvo, T.R., Barbastefano, V., Ferreira, A.L., Michelatto, D.D.P., De Almeida, A.C.A., Souza Brito, A.R.M., Antioxidant activity of indigo and its preventive effect against ethanol-induced DNA damage in rat gastric mucosa (2007) Planta Medica, 73 (12), pp. 1241-1246. , DOI 10.1055/s-2007-981613Grant, C.M., Role of the glutathione/glutaredoxin and thioredoxin systems in yeast growth and response to stress conditions (2001) Molecular Microbiology, 39 (3), pp. 533-541. , DOI 10.1046/j.1365-2958.2001.02283.xGrob, N.G., Peptic ulcer in twenty-century of America (2004) New Jersey Medicine, 101, pp. 19-28Jung, H.K., Lee, K.E., Chu, S.H., Yi, S.Y., Reactive oxygen species activity, mucosal lipoperoxidation and glutathione in Helicobacter pylori-infected gastric mucosa (2001) Journal of Gastroenterology and Hepatology, 16 (12), pp. 1336-1340. , DOI 10.1046/j.1440-1746.2001.02647.xKimura, M., Goto, S., Ihara, Y., Wada, A., Yahiro, K., Niidome, T., Aoyagi, H., Kondo, T., Impairment of glutathione metabolism in human gastric epithelial cells treated with vacuolating cytotoxin from Helicobacter pylori (2001) Microbial Pathogenesis, 31 (1), pp. 29-36. , DOI 10.1006/mpat.2001.0446Kvietys, P.R., Twohig, B., Danzell, J., Specian, R.D., Ethanol-induced injury to the rat gastric mucosa. Role of neutrophils and xanthine oxidase-derived radicals (1990) Gastroenterology, 98 (4), pp. 909-920Laine, L., Takeuchi, K., Tarnawski, A., Gastric Mucosal Defense and Cytoprotection: Bench to Bedside (2008) Gastroenterology, 135 (1), pp. 41-60. , DOI 10.1053/j.gastro.2008.05.030, PII S0016508508007944Malfertheiner, P., Chan, F.K., McColl, K.E., Peptic ulcer disease (2009) Lancet, 374, pp. 1449-1461Massignani, J., Lemos, M., Maistro, E.L., Schaphauser, H.P., Jorge, R.F., Sousa, J.P., Bastos, J.K., De Andrade, S.F., Antiulcerogenic activity of the essential oil of Baccharis dracunculifolia on different experimental models in rats (2009) Phytotherapy Research, 23, pp. 1355-1360Matos, F.J.A., (1999) Plantas da Medicina Popular Do Nordeste: Propriedades Atribuídas e Confirmadas, p. 78. , Editora UFC FortalezaMoreno, M.I.N., Isla, M.I., Sampietro, A.R., Vattuone, M.A., Comparison of the free radical-scavenging activity of propolis from several regions of Argentina (2000) Journal of Ethnopharmacology, 71 (1-2), pp. 109-114. , DOI 10.1016/S0378-8741(99)00189-0, PII S0378874199001890Morimoto, Y., Shimohara, K., Oshima, S., Sukamoto, T., Effects of the new anti-ulcer agent KB-5492 on experimental gastric mucosal lesions and gastric mucosal defensive factors, as compared to those of teprenone and cimetidine (1991) Japanese Journal of Pharmacology, 57, pp. 495-505Mota, K.S., Dias, G.E., Pinto, M.E., Luiz-Ferreira, A., Souza-Brito, A.R., Hiruma-Lima, C.A., Barbosa-Filho, J.M., Batista, L.M., Flavonoids with gastroprotective activity (2009) Molecules, 14, pp. 979-1012Naryzhny, S.N., Proliferating cell nuclear antigen: A proteomics view (2008) Cellular and Molecular Life Sciences, 65, pp. 3789-3808Okabe, S., Amagase, K., An overview of acetic acid ulcer models: The history and state of the art of peptic ulcer research (2005) Biological and Pharmaceutical Bulletin, 28 (8), pp. 1321-1341. , http://bpb.pharm.or.jp/bpb/200508/b08_1321.pdf, DOI 10.1248/bpb.28.1321Rafatullah, S., Tariq, M., Al-Yahya, M.A., Mossa, J.S., Ageel, A.M., Evaluation of turmeric (Curcuma longa) for gastric and duodenal antiulcer activity in rats (1990) Journal of Ethnopharmacology, 29 (1), pp. 25-34Roig, J.T., (1988) Plantas Medicinales, Aromaticas O Venenosas de Cuba, , Editorial Cientifica-Tecnica, La HavanaRokutan, K., Role of heat shock proteins in gastric mucosal protection (2000) Journal of Gastroenterology and Hepatology, 15, pp. 12-19Schmeda-Hirschmann, G., Yesilada, E., Traditional medicine and gastroprotective crude drugs (2005) Journal of Ethnopharmacology, 100 (1-2), pp. 61-66. , DOI 10.1016/j.jep.2005.06.002, PII S037887410500351X, Perspectives of EthnopharmacologyShay, H., Komarov, S.A., Fels, S.S., Meranze, D., Gruenstein, M., Siplet, H., A simple method for the uniform production of gastric ulceration in the rat (1945) Gastroenterology, 5, pp. 43-61Shichijo, K., Ihara, M., Matsuu, M., Ito, M., Okumura, Y., Sekine, I., Overexpression of heat shock protein 70 in stomach of stress-induced gastric ulcer-resistant rats (2003) Digestive Diseases and Sciences, 48 (2), pp. 340-348. , DOI 10.1023/A:1021939829515Sostres, C., Gargallo, C.J., Arroyo, M.T., Lanas, A., Adverse effects of non-steroidal anti-inflammatory drugs (NSAIDs, aspirin and coxibs) on upper gastrointestinal tract. Best Practice and Research (2010) Clinical Gastroenterology, 24, pp. 121-132Szelenyi, I., Thiemer, K., Distention ulcer as a model for testing of drugs for ulcerogenic side effects (1978) Archives of Toxicology, 41 (1), pp. 99-105Takagi, K., Okabe, S., Saziki, R., A new method for the production of chronic gastric ulcer in rats and the effect of several drugs on its healing (1969) Japanese Journal of Pharmacology, 19, pp. 418-426Tarnawski, A., Cellular and molecular mechanisms of gastrointestinal ulcer healing (2005) Digestive Diseases and Sciences, 50, pp. 24-S33Tarnawski, A., Stachura, J., Krause, W.J., Douglass, T.G., Gergely, H., Quality of gastric ulcer healing: A new, emerging concept (1991) Journal of Clinical Gastroenterology, 13, pp. 42-S47Tsukimi, Y., Nakai, H., Itoh, S., Amagase, K., Okabe, S., Involvement of heat shock proteins in the healing of acetic acid-induced gastric ulcers in rats (2001) Journal of Physiology and Pharmacology, 52 (3), pp. 391-406Vacca, L.L., (1985) Laboratory Manual of Histochemistry, pp. 176-224. , Raven Press New YorkVieira, J.R., De Souza, I.A., Do Nascimento, S.C., Leite, S.P., Indigofera suffruticosa: An alternative anticancer therapy (2007) Evidence-Based Complementary and Alternative Medicine, 4, pp. 355-35