Immunization with Recombinant Surface Antigen P50 of Babesia gibsoni Expressed in Insect Cells Induced Parasite Growth Inhibition in Dogs

This is a report of a vaccine trial directed against Babesia gibsoni infection in dogs with the use of the recombinant antigen P50. Dogs immunized with P50 showed partial protection manifested as a significantly low level of parasitemia. The results indicated that P50 is a primary vaccine candidate molecule against canine B. gibsoni infection

Occurrence of bovine ephemeral fever in Okinawa Prefecture, Japan, in 2012 and development of a reverse-transcription polymerase chain reaction assay to detect bovine ephemeral fever virus gene

En el presente trabajo realizaremos un análisis sobre de las dinámicas mediante las cuales se han desarrollado los Talleres de la cátedra de Historia Social Argentina y Latinoamericana en la Facultad de Trabajo Social, y en particular sobre los registros de las estudiantes en el taller de Convenios Colectivos de Trabajo. Nuestro objetivo es compartir el valor de las experiencias construidas para la formación profesional en Trabajo Social, deteniéndonos en las formas en que se constituyeron los distintos talleres, a través de las perspectivas y discusiones debatidas sobre el mundo del Trabajo -y su relación con el Trabajo Social-, partiendo de las intervenciones orales y escritas de las y los estudiantes. Los Talleres forman parte del diseño de la Propuesta Pedagógica de la materia. Son parte también del proceso de evaluación, obligatorios para quienes aspiran a aprobar mediante el régimen de promoción sin examen final y, al igual que el resto de las clases, abiertos a la participación pública. Los talleres suelen constituirse con una asistencia aproximada de doscientas personas, en su mayoría mujeres, condición que se reproduce a lo largo de la carrera de Trabajo Social.Eje temático 3: La educación superior como derecho: prácticas de enseñanza, investigación y extensión.Grupo de trabajo 18: La educación superior como derecho: prácticas de enseñanza, investigación y extensión.Facultad de Trabajo Socia

Data from: Japanese encephalitis in a 114-month-old cow: pathological investigation of the affected cow and genetic characterization of Japanese encephalitis virus isolate

Background: Japanese encephalitis virus (JEV) is classified into the genus Flavivirus in the family Flaviviridae. JEV can cause febrile illness and encephalitis mainly in humans and horses, and occasionally in cattle. Case presentation: In late September 2010, a 114-month-old cow showed neurological symptoms similar to the symptoms observed in previous bovine cases of Japanese encephalitis (JE); therefore, we conducted virological and pathological tests on the cow. As a result, JEV was isolated from the cerebrum of the affected cow. We determined the complete genome sequence of the JEV isolate, which we named JEV/Bo/Aichi/1/2010, including the envelope (E) gene region and 3' untranslated region (3'UTR). Our phylogenetic analyses of the E region and complete genome showed that the isolate belongs to JEV genotype 1 (G1). The isolate, JEV/Bo/Aichi/1/2010, was most closely related to several JEV G1 isolates in Toyama Prefecture, Japan in 2007-2009 by the phylogenetic analysis of the E region. In addition, the nucleotide alignment revealed that the deletion in the 3'UTR was the same between JEV/Bo/Aichi/1/2010 and several other JEV G1 isolates identified in Toyama Prefecture in 2008-2009. A hemagglutination inhibition (HI) test was conducted for the detection of anti-JEV antibodies in the affected cow, and the test detected 2-mercaptoethanol (2-ME)-sensitive HI antibodies against JEV in the serum of the affected cow. The histopathological investigation revealed nonsuppurative encephalomyelitis in the affected cow, and the immunohistochemical assay detected JEV antigen in the cerebrum. Conclusion: We diagnosed the case as JE of a cow based on the findings of nonsuppurative encephalomyelitis observed in the central nervous system, JEV antigen detected in the cerebrum, JEV isolated from the cerebrum, and 2-ME-sensitive HI antibodies against JEV detected in the serum. This is the first reported case of JE in a cow over 24 months old