Dual-Frequency VSOP Observations of AO 0235+164

AO 0235+164 is a very compact, flat spectrum radio source identified as a BL Lac object at a redshift of z=0.94. It is one of the most violently variable extragalactic objects at both optical and radio wavelengths. The radio structure of the source revealed by various ground-based VLBI observations is dominated by a nearly unresolved compact component at almost all available frequencies. Dual-frequency space VLBI observations of AO 0235+164 were made with the VSOP mission in January-February 1999. The array of the Japanese HALCA satellite and co-observing ground radio telescopes in Australia, Japan, China and South Africa allowed us to study AO 0235+164 with an unprecedented angular resolution at frequencies of 1.6 and 5 GHz. We report on the sub-milliarcsecond structural properties of the source. The 5-GHz observations led to an estimate of T_B > 5.8 x 10^{13} K for the rest-frame brightness temperature of the core, which is the highest value measured with VSOP to date.Comment: 8 pages, 8 figures, to appear in Publ. Astron. Soc. Japa

Microtubule assembly and in vitro development of bovine oocytes with increased intracellular glutathione level prior to vitrification and in vitro fertilization

Although vitrification is a useful technique for preservation of bovine oocytes, the yield of blastocysts derived from the vitrified oocytes is still low. We have recently reported a new type of cryoinjury, multiple aster formation, by which pronuclear migration and development of vitrified–warmed and in vitro-fertilized bovine oocytes are impaired. The aim of the present study was to investigate the effect of glutathione (GSH) content of vitrified bovine oocytes on multiple aster formation and subsequent in vitro development. Treatment of bovine cumulus–oocyte complexes with β-mercaptoethanol (βME) and l-cysteine (Cys) during in vitro maturation resulted in 2.5-fold higher GSH content not only in fresh control but also in vitrified–warmed oocytes. The percentage of normally fertilized zygotes exhibiting sperm aster(s) was >95% in all four groups (with or without βME/Cys × fresh control or vitrified). The frequency of multiple aster formation in vitrified oocytes (three-fold higher than that in fresh control oocytes) was not affected by the increased level of intracellular GSH with βME/Cys. Consequently, the migration and development of pronuclei as well as the yield of blastocysts from vitrified–warmed oocytes (17 versus 41%) were not improved. In addition, there was no effect of increased GSH level on the yield of blastocysts in fresh control groups.ArticleZYGOTE. 22(4):476-482 (2014)journal articl

High incidence of multiple aster formation in vitrified-warmed bovine oocytes after in vitro fertilization

In vitro-matured bovine oocytes do not tolerate vitrification as well as mature murine or human oocytes. Delayed first cleavage in vitrified and in vitro-fertilized bovine oocytes may be responsible for the decreased yield of blastocysts in vitro. Because formation of sperm-aster and the subsequent assembly of microtubule network play an important role for migration and fusion of both pronuclei, aster formation in vitrified-warmed oocytes was analyzed by confocal laser-scanning microscopy. At 10 h post-insemination (hpi), proportions of oocytes fertilized normally were comparable between the vitrified and fresh control groups (67 and 70%, respectively). Proportions of oocytes that exhibited microtubule assembly were similar between the two groups (95% each), but the proportion of oocytes with multiple asters was higher in the vitrified group when compared with the fresh control group (68 vs 29%, P < 0.05). Both migration and development of two pronuclei were adversely affected by multiple aster formation. In the next experiment, multiple asters observed in 5.5 vs 8 hpi pronuclear zygotes were located near the male pronucleus, suggesting that those multiple asters were not the cytoplasmic asters of maternal origin. In conclusion, multiple aster formation frequently observed in vitrified-warmed bovine oocytes may be related to loss of ooplasmic function responsible for normal microtubule assembly from the sperm-aster.ArticleTHERIOGENOLOGY. 77(5):908-915 (2012)journal articl

A relativistic helical jet in the gamma-ray AGN 1156+295

We present the results of a number of high resolution radio observations of the AGN 1156+295. These include multi-epoch and multi-frequency VLBI, VSOP, MERLIN and VLA observations made over a period of 50 months. The 5 GHz MERLIN images trace a straight jet extending to 2 arcsec at P.A. -18 degrees. Extended low brightness emission was detected in the MERLIN observation at 1.6 GHz and the VLA observation at 8.5 GHz with a bend of about 90 degrees at the end of the 2 arcsecond jet. A region of similar diffuse emission is also seen about 2 arcseconds south of the radio core. The VLBI images of the blazar reveal a core-jet structure with an oscillating jet on a milli-arcsecond (mas) scale which aligns with the arcsecond jet at a distance of several tens of milli-arcseconds from the core. This probably indicates that the orientation of the jet structure is close to the line of sight, with the northern jet being relativistically beamed toward us. In this scenario the diffuse emission to the north and south is not beamed and appears symmetrical. For the northern jet at the mas scale, proper motions of 13.7 +/-3.5, 10.6 +/- 2.8, and 11.8 +/- 2.8 c are measured in three distinct components of the jet (q_0=0.5, H_0=65 km /s /Mpc are used through out this paper). Highly polarised emission is detected on VLBI scales in the region in which the jet bends sharply to the north-west. The spectral index distribution of the source shows that the strongest compact component has a flat spectrum, and the extended jet has a steep spectrum. A helical trajectory along the surface of a cone was proposed based on the conservation laws for kinetic energy and momentum to explain the observed phenomena, which is in a good agreement with the observed results on scales of 1 mas to 1 arcsec.Comment: 19 pages with 18 figures. Accepted for publication in the A&

Multi-frequency investigation of the parsec- and kilo-parsec-scale radio structures in high-redshift quasar PKS 1402+044

We investigate the frequency-dependent radio properties of the jet of the luminous high-redshift (z = 3.2) radio quasar PKS 1402+044 (J1405+0415) by means of radio interferometric observations. The observational data were obtained with the VLBI Space Observatory Programme (VSOP) at 1.6 and 5 GHz, supplemented by other multi-frequency observations with the Very Long Baseline Array (VLBA; 2.3, 8.4, and 15 GHz) and the Very Large Array (VLA; 1.4, 5, 15, and 43 GHz). The observations span a period of 7 years. We find that the luminous high-redshift quasar PKS 1402+044 has a pronounced "core-jet" morphology from the parsec to the kilo-parsec scales. The jet shows a steeper spectral index and lower brightness temperature with increasing distance from the jet core. The variation of brightness temperature agrees well with the shock-in-jet model. Assuming that the jet is collimated by the ambient magnetic field, we estimate the mass of the central object as ~10^9 M_sun. The upper limit of the jet proper motion of PKS 1402+044 is 0.03 mas/yr (~3c) in the east-west direction.Comment: 9 pages, 6 figures

Laser gas-discharge absorption measurements of the ratio of two transition rates in argon

The ratio of two line strengths at 922.7 nm and 978.7 nm of argon is measured in an argon pulsed discharge with the use of a single-mode Ti:Sapphire laser. The result 3.29(0.13) is in agreement with our theoretical prediction 3.23 and with a less accurate ratio 2.89(0.43) from the NIST database.Comment: 5 pages, 2 figures, 1 tabl

Assessment of cervical myelopathy using transcranial magnetic stimulation and prediction of prognosis after laminoplasty

This is a non-final version of an article published in final form in SPINE 33(1): E15-E20, 2008.http://www.spinejournal.com/pt/re/spine/home | http://www.spinejournal.com/pt/re/spine/homeArticleSPINE. 33(1): E15-E20 (2008)journal articl

The impacts of climate change on river flood risk at the global scale

This paper presents an assessment of the implications of climate change for global river flood risk. It is based on the estimation of flood frequency relationships at a grid resolution of 0.5 × 0.5°, using a global hydrological model with climate scenarios derived from 21 climate models, together with projections of future population. Four indicators of the flood hazard are calculated; change in the magnitude and return period of flood peaks, flood-prone population and cropland exposed to substantial change in flood frequency, and a generalised measure of regional flood risk based on combining frequency curves with generic flood damage functions. Under one climate model, emissions and socioeconomic scenario (HadCM3 and SRES A1b), in 2050 the current 100-year flood would occur at least twice as frequently across 40 % of the globe, approximately 450 million flood-prone people and 430 thousand km2 of flood-prone cropland would be exposed to a doubling of flood frequency, and global flood risk would increase by approximately 187 % over the risk in 2050 in the absence of climate change. There is strong regional variability (most adverse impacts would be in Asia), and considerable variability between climate models. In 2050, the range in increased exposure across 21 climate models under SRES A1b is 31–450 million people and 59 to 430 thousand km2 of cropland, and the change in risk varies between −9 and +376 %. The paper presents impacts by region, and also presents relationships between change in global mean surface temperature and impacts on the global flood hazard. There are a number of caveats with the analysis; it is based on one global hydrological model only, the climate scenarios are constructed using pattern-scaling, and the precise impacts are sensitive to some of the assumptions in the definition and application