Aston University's Antimicrobial Resistance (AMR) Roadshow: raising awareness and embedding knowledge of AMR in key stage 4 learners:raising awareness and embedding knowledge of AMR in key stage 4 learners

Antimicrobial resistance (AMR) is a global healthcare problem and therefore raising awareness within young learners is imperative. An AMR roadshow was designed to take key stage 4 students' learning ‘out of the classroom’, assess pre-existing knowledge of AMR and determine the impact of the roadshow on knowledge retention. Knowledge and subsequent retention were measured pre- and post-event through a standardised questionnaire. The roadshow significantly improved knowledge and understanding of AMR, which was retained for a minimum of twelve weeks. Engaging and interactive strategies addressing key health issues provide a positive learning experience which contributes to retained knowledge in young learners

Woody biomass increases across three contrasting land uses in Hurungwe, mid-Zambezi valley, Zimbabwe

Globally, Miombo woodlands store important quantities of carbon, with tree cover and carbon stocks strongly determined by human use. We assessed woodland cover and aboveground carbon (AGC) stocks of miombo along a utilisation gradient on three different land use types, that is, a national park, a buffer zone and a communal area. Woodland cover and carbon stock changes were assessed through mapping of AGC between 2007 and 2017 using Phased Array L-Band Synthetic Aperture Radar observations (ALOS-PALSAR 1 and 2). Woodland cover was higher in the national park and the buffer zone than in the communal area for both 2007 and 2017. In 2007, mean AGC stock was not significantly different (p = 0.005) across all three land use types. However, in 2017, mean AGC was significantly lower (p < 0.001) in the buffer zone and communal area than in the national park. In all three land use types, Miombo woodland cover and mean AGC gains outweighed losses over the 10-year period. AGC gains were significantly higher (p < 0.001) in the national park than in both the buffer zone and the communal area. Results of the study indicate that woodland cover and aboveground carbon increased in all three land use types despite the observed human disturbance over the study period. Both variables recorded a lower increase with elevated utilisation. The study concluded that sustainable resource utilisation is possible without loss of such ecosystem services as carbon sequestration and climate change mitigation

Antibody recognizing 4-sulfated chondroitin sulfate proteoglycans restores memory in tauopathy-induced neurodegeneration

Chondroitin sulfate proteoglycans (CSPGs) are the main active component of perineuronal nets (PNNs). Digestion of the glycosaminoglycan chains of CSPGs with chondroitinase ABC or transgenic attenuation of PNNs leads to prolongation of object recognition memory and activation of various forms of plasticity in the adult central nervous system. The inhibitory properties of the CSPGs depend on the pattern of sulfation of their glycosaminoglycans, with chondroitin 4-sulfate (C4S) being the most inhibitory form. In this study, we tested a number of candidates for functional blocking of C4S, leading to selection of an antibody, Cat316, which specifically recognizes C4S and blocks its inhibitory effects on axon growth. It also partly blocks binding of semaphorin 3A to PNNs and attenuates PNN formation. We asked whether injection of Cat316 into the perirhinal cortex would have the same effects on memory as chondroitinase ABC treatment. We found that masking C4S with the Cat316 antibody extended long-term object recognition memory in normal wild-type mice to 24 hours, similarly to chondroitinase or transgenic PNN attenuation. We then tested Cat316 for restoration of memory in a neurodegeneration model. Mice expressing tau with the P301S mutation showed profound loss of object recognition memory at 4 months of age. Injection of Cat316 into the perirhinal cortex normalized object recognition at 3 hours in P301S mice. These data indicate that Cat316 binding to C4S in the extracellular matrix can restore plasticity and memory in the same way as chondroitinase ABC digestion. Our results suggest that antibodies to C4S could be a useful therapeutic to restore memory function in neurodegenerative disorders.This work was supported by the ERC advanced grant ECM Neuro (294502), by a fellowship to SY from Alzheimer's Research UK (ARUK-RF2016A-1), and by the NIHR Cambridge Biomedical Research Centre

Multishank Thin-Film Neural Probes and Implantation System for High-Resolution Neural Recording Applications

Abstract Silicon probes have played a key role in studying the brain. However, the stark mechanical mismatch between these probes and the brain leads to chronic damage in the surrounding neural tissue, limiting their application in research and clinical translation. Mechanically flexible probes made of thin plastic shanks offer an attractive tissue‐compatible alternative but are difficult to implant into the brain. They also struggle to achieve the electrode density and layout necessary for the high‐resolution applications their silicon counterparts excel at. Here, a multishank high‐density flexible neural probe design is presented, which emulates the functionality of stiff silicon arrays for recording from neural population across multiple sites within a given region. The flexible probe is accompanied by a detachable 3D printed implanter, which delivers the probe by means of hydrophobic‐coated shuttles. The shuttles can then be retracted with minimal movement and the implanter houses the electronics necessary for in vivo recording applications. Validation of the probes through extracellular recordings from multiple brain regions and histological evidence of minimal foreign body response opens the path to long‐term chronic monitoring of neural ensembles

Long-term protection against HBV chronic carriage of Gambian adolescents vaccinated in infancy and immune response in HBV booster trial in adolescence.

BACKGROUND: Chronic infection with hepatitis B virus (HBV) arising in childhood is associated with hepatocellular carcinoma in adult life. Between 1986 and 1990, approximately 120,000 Gambian newborns were enrolled in a randomised controlled trial to assess the effectiveness of infant HBV vaccination on the prevention of hepatocellular carcinoma in adulthood. These children are now in adolescence and approaching adulthood, when the onset of sexual activity may challenge their hepatitis B immunity. Thus a booster dose in adolescence could be important to maintain long-term protection. METHODS: Fifteen years after the start of the HBV infant vaccination study, 492 vaccinated and 424 unvaccinated children were identified to determine vaccine efficacy against infection and carriage in adolescence. At the same time, 297 of the 492 infant-vaccinated subjects were randomly offered a booster dose of HBV vaccine. Anti-HBs was measured before the booster, and two weeks and 1 year afterwards (ISRCTN71271385). RESULTS: Vaccine efficacy 15 years after vaccination was 67.0% against infection as manifest by anti-HBc positivity (95% CI 58.2-74.6%), and 96.6% against HBsAg carriage (95% CI 91.5-100%). 31.2% of participants had detectable anti-HBs with a GMC of 32 IU/l. For 168 boosted participants GMC anti-HBs responses were 38 IU/l prior to vaccination, 524 IU/l two weeks after boosting, and 101 IU/l after 1 year. CONCLUSIONS: HBV vaccination in infants confers very good protection against carriage up to 15 years of age, although a large proportion of vaccinated subjects did not have detectable anti-HBs at this age. The response to boosting persisted for at least a year. TRIAL REGISTRATION: Controlled-Trials.com ISRCTN71271385

Impact of baryons on the cluster mass function and cosmological parameter determination

Recent results by the Planck collaboration have shown that cosmological parameters derived from the cosmic microwave background anisotropies and cluster number counts are in tension, with the latter preferring lower values of the matter density parameter, $\Omega_\mathrm{m}$, and power spectrum amplitude, $\sigma_8$. Motivated by this, we investigate the extent to which the tension may be ameliorated once the effect of baryonic depletion on the cluster mass function is taken into account. We use the large-volume Millennium Gas simulations in our study, including one where the gas is pre-heated at high redshift and one where the gas is heated by stars and active galactic nuclei (in the latter, the self-gravity of the baryons and radiative cooling are omitted). In both cases, the cluster baryon fractions are in reasonably good agreement with the data at low redshift, showing significant depletion of baryons with respect to the cosmic mean. As a result, it is found that the cluster abundance in these simulations is around 15 per cent lower than the commonly-adopted fit to dark matter simulations by Tinker et al (2008) for the mass range $10^{14}-10^{14.5}h^{-1} \mathrm{M}_\odot$. Ignoring this effect produces a significant artificial shift in cosmological parameters which can be expressed as $\Delta[\sigma_8(\Omega_\mathrm{m}/0.27)^{0.38}]\simeq -0.03$ at $z=0.17$ (the median redshift of the $\mathit{Planck}$ cluster sample) for the feedback model. While this shift is not sufficient to fully explain the $\mathit{Planck}$ discrepancy, it is clear that such an effect cannot be ignored in future precision measurements of cosmological parameters with clusters. Finally, we outline a simple, model-independent procedure that attempts to correct for the effect of baryonic depletion and show that it works if the baryon-dark matter back-reaction is negligible.Comment: 10 pages, 5 figures, Accepted by MNRA

Aflatoxin Exposure and Viral Hepatitis in the Etiology of Liver Cirrhosis in The Gambia, West Africa

BACKGROUND: Cirrhosis of the liver is thought to be a major cause of morbidity and mortality in sub-Saharan Africa, but few controlled studies on the etiology of cirrhosis have been conducted in this region. OBJECTIVES: We aimed to elucidate the association between environmental and infectious exposures and cirrhosis in The Gambia. METHODS: Ninety-seven individuals were diagnosed with cirrhosis using a validated ultrasound scoring system and were compared with 397 controls. Participants reported demographic and food frequency information. Blood samples were tested for hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), hepatitis C virus (HCV) antibody, HCV RNA, and the aflatoxin-associated 249(ser) TP53 mutation. RESULTS: HBsAg seropositivity was associated with a significant increase in risk of cirrhosis [odds ratio (OR) = 8.0; 95% confidence interval (CI), 4.4-14.7] as was the presence of HBeAg (OR = 10.3; 95% CI, 2.0-53.9) and HCV infection (OR = 3.3; 95% CI, 1.2-9.5). We present novel data that exposure to aflatoxin, as assessed both by high lifetime groundnut (peanut) intake and by the presence of the 249(ser) TP53 mutation in plasma, is associated with a significant increase in the risk for cirrhosis (OR = 2.8; 95% CI, 1.1-7.7 and OR = 3.8; 95% CI, 1.5-9.6, respectively). Additionally, aflatoxin and hepatitis B virus exposure appeared to interact synergistically to substantially increase the risk of cirrhosis, although this was not statistically significant. CONCLUSIONS: Our results suggest that the spectrum of morbidity associated with aflatoxin exposure could include cirrhosis