Pengaruh Kesadaran Merek, Asosiasi Merek dan Persepsi Kualitas terhadap Keputusan Pembelian Pc Tablet Samsung Galaxi di Kota Padang

Penelitian ini bertujuan untuk menganalisis pengaruh Kesadaran merek, asosiasi merek dan persepsi kualitas terhadap keputusan pembelian PC tablet Samsung galaxi di kota padang. Jenis penelitian ini adalah penelitian kausatif yang bertujuan untuk mengetahui pengaruh Kesadaran merek, asosiasi merek dan persepsi kualitas terhadap keputusan pembelian PC tablet Samsung galaxi di kota padang. Populasi dalam penelitian ini adalah konsumen yang pernah menggunakan PC Tablet Samsung Galaxi di Kota Padang. Pengambilan sampel dengan menggunakan metode accidental sampling, yaitu memilih sampel berdasarkan elemen populasi (orang atau kejadian) yang datanya mudah diperoleh peneliti. Analisis data yang digunakan adalah analisis regresi berganda dan uji hipotesis dengan menggunakan uji t.Hasil penelitian ini menunjukkan bahwa : Secara parsial kesadaran merek, asosiasi merek dan persepsi kaulitas berpengaruh terhadap keputusan pembelian PC Tablet Samsung Galaxi di Kota Padang. Selanjutnya kontribusi sumbangan variabel Kesadaran merek, asosiasi merek, persepsi kualitas terhadap keputusan pembelian PC Tablet Samsung Galaxi di Kota Padang adalah sebesar 77,5%. Sedangkan 22,5 dipengaruhi oleh variabel lain. Jadi, selain kesadaran merek, asosiasi merek, dan persepsi kualitas terdapat juga pengaruh faktor lain yang mempengaruhi keputusan pembelian.Kata Kunci : Kesadaran Merek, Asosiasi Merek, Persepsi Kualitas, Keputusan Pembelia

Pengaruh Gaya Belajar dan Pemanfaatan Media Pembelajaran terhadap Hasil Belajar Ekonomi pada Siswa Kelas X SMA N 5 Solok Selatan

This research was conducted at SMAN 5 South Solok regarding the influence of emotional intelligence and computer -based media pemanfataan the results belajajar grader sma x 5 South Solok country in economic subjects . The purpose of this study is to obtain empirical evidence about the influence of emotional intelligence and computer -based media pemanfataan the class X results belajajar SMA 5 South Solok in economic subjects .The method used in this research is descriptive and quantitative methods . where the population is students and as many as 182 samples used were as many as 125 students with proportional sampling technique of random sampling . Learning style data ( X1 ) and pemanfataan learning media ( X2 ) is obtained from the results of the questionnaire or the questionnaire while the student learning outcomes data ( Y ) is obtained from the daily student test scores . Analysis of the data used in this study is a descriptive analysis and inductive analysis which consists of classical assumption test , multiple regression analysis and hypothesis testing .The results of this study indicate that : 1 . Learning style significant and positive impact on learning outcomes economy class X SMA Negeri 5 South Solok , 2 . Utilization media student learning positive and significant effect on learning outcomes economy class X SMA Negeri 5 South Solok , 3 . Styles of learning and teaching media pemanfataan simultaneously significant effect on learning outcomes economy class X SMA Negeri 5 South Solok . Based on these results , it is suggested that the school , teachers , and students to pay more attention to students learning styles such as visual learning style , auditory learning styles and learning styles and pemanfataan kirestetik existing instructional media that has been provided by the school in the learning process in an effort to further optimize the learning outcomes of the economy class X SMA Negeri 5 South Solok

tRNA-mediated codon-biased translation in mycobacterial hypoxic persistence

Microbial pathogens adapt to the stress of infection by regulating transcription, translation and protein modification. We report that changes in gene expression in hypoxia-induced non-replicating persistence in mycobacteria—which models tuberculous granulomas—are partly determined by a mechanism of tRNA reprogramming and codon-biased translation. Mycobacterium bovis BCG responded to each stage of hypoxia and aerobic resuscitation by uniquely reprogramming 40 modified ribonucleosides in tRNA, which correlate with selective translation of mRNAs from families of codon-biased persistence genes. For example, early hypoxia increases wobble cmo[superscript 5]U in tRNA[superscript Thr(UGU)], which parallels translation of transcripts enriched in its cognate codon, ACG, including the DosR master regulator of hypoxic bacteriostasis. Codon re-engineering of dosR exaggerates hypoxia-induced changes in codon-biased DosR translation, with altered dosR expression revealing unanticipated effects on bacterial survival during hypoxia. These results reveal a coordinated system of tRNA modifications and translation of codon-biased transcripts that enhance expression of stress response proteins in mycobacteria.Singapore-MIT Alliance for Research and Technology (SMART)National Institute of Environmental Health Sciences (grants ES017010 and ES002109)National Science Foundation (U.S.) (grant CHE-1308839)Singapore-MIT Alliance for Research and Technology (SMART) (SMA3 Graduate Fellowship

2′-O Methylation of Internal Adenosine by Flavivirus NS5 Methyltransferase

RNA modification plays an important role in modulating host-pathogen interaction. Flavivirus NS5 protein encodes N-7 and 2′-O methyltransferase activities that are required for the formation of 5′ type I cap (m7GpppAm) of viral RNA genome. Here we reported, for the first time, that flavivirus NS5 has a novel internal RNA methylation activity. Recombinant NS5 proteins of West Nile virus and Dengue virus (serotype 4; DENV-4) specifically methylates polyA, but not polyG, polyC, or polyU, indicating that the methylation occurs at adenosine residue. RNAs with internal adenosines substituted with 2′-O-methyladenosines are not active substrates for internal methylation, whereas RNAs with adenosines substituted with N6-methyladenosines can be efficiently methylated, suggesting that the internal methylation occurs at the 2′-OH position of adenosine. Mass spectroscopic analysis further demonstrated that the internal methylation product is 2′-O-methyladenosine. Importantly, genomic RNA purified from DENV virion contains 2′-O-methyladenosine. The 2′-O methylation of internal adenosine does not require specific RNA sequence since recombinant methyltransferase of DENV-4 can efficiently methylate RNAs spanning different regions of viral genome, host ribosomal RNAs, and polyA. Structure-based mutagenesis results indicate that K61-D146-K181-E217 tetrad of DENV-4 methyltransferase forms the active site of internal methylation activity; in addition, distinct residues within the methyl donor (S-adenosyl-L-methionine) pocket, GTP pocket, and RNA-binding site are critical for the internal methylation activity. Functional analysis using flavivirus replicon and genome-length RNAs showed that internal methylation attenuated viral RNA translation and replication. Polymerase assay revealed that internal 2′-O-methyladenosine reduces the efficiency of RNA elongation. Collectively, our results demonstrate that flavivirus NS5 performs 2′-O methylation of internal adenosine of viral RNA in vivo and host ribosomal RNAs in vitro

Outcomes from elective colorectal cancer surgery during the SARS-CoV-2 pandemic

This study aimed to describe the change in surgical practice and the impact of SARS-CoV-2 on mortality after surgical resection of colorectal cancer during the initial phases of the SARS-CoV-2 pandemic

Proximity to overhead power lines and childhood leukaemia: an international pooled analysis

© 2018, Cancer Research UK. Background: Although studies have consistently found an association between childhood leukaemia risk and magnetic fields, the associations between childhood leukaemia and distance to overhead power lines have been inconsistent. We pooled data from multiple studies to assess the association with distance and evaluate whether it is due to magnetic fields or other factors associated with distance from lines. Methods: We present a pooled analysis combining individual-level data (29,049 cases and 68,231 controls) from 11 record-based studies. Results: There was no material association between childhood leukaemia and distance to nearest overhead power line of any voltage. Among children living < 50 m from 200 + kV power lines, the adjusted odds ratio for childhood leukaemia was 1.33 (95% CI: 0.92–1.93). The odds ratio was higher among children diagnosed before age 5 years. There was no association with calculated magnetic fields. Odds ratios remained unchanged with adjustment for potential confounders. Conclusions: In this first comprehensive pooled analysis of childhood leukaemia and distance to power lines, we found a small and imprecise risk for residences < 50 m of 200 + kV lines that was not explained by high magnetic fields. Reasons for the increased risk, found in this and many other studies, remains to be elucidated