Análise físico-química de salames coloniais comercializados no município de Toledo, Estado do Paraná

Physical-chemical analysis of colonial italian type salami commercialized in Toledo, Parana state. Italian immigration in the west of Parana State contributed for the development of small meat industries. the colonial Italian-type salami is one of the main commercialized meat products in this region, therefore making necessary its quality evaluation. the aim of this work is to verify whether the composition of the colonial salami is in accordance with Brazilian law, and the nutritional facts information on the labels of these products. Four different marks of colonial Italian-type salami produced in the region of Toledo, Parana State and one great trade mark of Italian-type salami were herein evaluated. Properties like moisture, percentages of proteins, ashes, lipids and cholesterol were evaluated. We recorded a great discrepancy between values presented in the nutritional facts information and determined values, except for the industrialized salami. the moisture content was higher than the maximum levels recommended by the legislation. On the other hand, protein contents were lower than the minimum values recommended, and only the fat content matched the values established by Brazilian law. the cholesterol contents were lower than described on the labels when they contain them. According to the composition of the product and the Brazilian legislation, such salami could not be classified as Italian-type salami.Universidade Federal de São Paulo, BR-09972270 São Paulo, BrazilUniv Estadual Oeste Parana, Toledo, Parana, BrazilUniversidade Federal de São Paulo, BR-09972270 São Paulo, BrazilWeb of Scienc

Análise físico-química de salames coloniais comercializados no município de Toledo, Estado do Paraná=Physical-chemical analysis of colonial italian type salami commercialized in Toledo, Paraná state.

A imigração italiana no Oeste do Paraná contribuiu para o desenvolvimento de pequenas indústrias de produtos cárneos. O salame italiano colonial é um importante produto cárneo comercializado na região Oeste do Paraná, neste sentido é necessária a avaliação da qualidade deste produto. O objetivo deste trabalho é verificar se a composição centesimal destes salames coloniais está em conformidade com a legislação brasileira e com as informações contidas nos rótulos destes produtos. Quatro diferentes marcas de salames coloniais produzidos na região de Toledo, Estado do Paraná, e uma marca de salame industrializado foram avaliadas, neste trabalho, quanto aos teores de umidade, cinzas, proteínas, lipídios e colesterol. Os resultados mostraram que há diferença entre os valores apresentados nas informações nutricionais do rótulo e os valores determinados neste trabalho, com exceção do salame industrializado. Os teores de umidade foram maiores do que os teores máximos preconizados pela legislação. Por outro lado, os teores de proteínas foram menores que os valores mínimos, mas apenas os teores de gordura concordaram com os valores exigidos pela legislação brasileira. As análises de colesterol mostraram que os valores são inferiores àqueles descritos nos rótulos quando os mesmos os contêm. De acordo com as composições dos salames e a legislação brasileira, tais salames não poderiam ser enquadrados como salames italianos.<br><br>Italian immigration in the west of Paraná State contributed for the development of small meat industries. The colonial Italian-type salami is one of the main commercialized meat products in this region, therefore making necessary its quality evaluation. The aim of this work is to verify whether the composition of the colonial salami is in accordance with Brazilian law, and the nutritional facts information on the labels of these products. Four different marks of colonial Italian-type salami produced in the region of Toledo, Paraná State and one great trade mark of Italian-type salami were herein evaluated. Properties like moisture, percentages of proteins, ashes, lipids and cholesterol were evaluated. We recorded a great discrepancy between values presented in the nutritional facts information and determined values, except for the industrialized salami. The moisture content was higher than the maximum levels recommended by the legislation. On the other hand, protein contents were lower than the minimum values recommended, and only the fat content matched the values established by Brazilian law. The cholesterol contents were lower than described on the labels when they contain them. According to the composition of the product and the Brazilian legislation, such salami could not be classified as Italian-type salami

Microfluidic-Based Genosensor To Detect Human Papillomavirus (HPV16) for Head and Neck Cancer

Just Accepted ManuscriptHigh-risk human papillomavirus (HPV) infection, mainly with HPV16 type, has been increasingly considered as an important etiologic factor in head and neck cancers. Detection of HPV16 is therefore crucial for these types of cancer, but clinical tests are not performed routinely in public health systems owing to the high cost and limitations of the existing tests. In this article, we report on a potentially low-cost genosensor capable of detecting low concentrations of HPV16 in buffer samples and distinguishing, with high accuracy, head and neck cancer cell lines according to their HPV16 status. The genosensor consisted of a microfluidic device that had an active layer of a HPV16 capture DNA probe (cpHPV16) deposited onto a layer-by-layer film of chitosan and chondroitin sulfate. Impedance spectroscopy was the principle of detection utilized, leading to a limit of detection of 10.5 pM for complementary ssDNA HPV16 oligos (ssHPV16). The genosensor was also able to distinguish among HPV16+ and HPV16- cell lines, using the multidimensional projection technique interactive document mapping. Hybridization between the ssHPV16 oligos and cpHPV16 probe was confirmed with polarization-modulated infrared reflection-absorption spectroscopy, where PO2 and amide I and amide II bands from adenine and thymine were monitored. The electrical response could be modeled as resulting from an adsorption process represented in a Freundlich model. Because the fabrication procedures of the microfluidic devices and genosensors and the data collection and analysis can be implemented at low cost, the results presented here amount to a demonstration of possible routine screening for HPV infections.CNPq (150985/2017-7), FAPESP (2013/14262-7) and Barretos Cancer Hospitalinfo:eu-repo/semantics/publishedVersio