Protein-based materials, toward a new level of structural control

Through billions of years of evolution nature has created and refined structural proteins for a wide variety of specific purposes. Amino acid sequences and their associated folding patterns combine to create elastic, rigid or tough materials. In many respects, nature’s intricately designed products provide challenging examples for materials scientists, but translation of natural structural concepts into bio-inspired materials requires a level of control of macromolecular architecture far higher than that afforded by conventional polymerization processes. An increasingly important approach to this problem has been to use biological systems for production of materials. Through protein engineering, artificial genes can be developed that encode protein-based materials with desired features. Structural elements found in nature, such as β-sheets and α-helices, can be combined with great flexibility, and can be outfitted with functional elements such as cell binding sites or enzymatic domains. The possibility of incorporating non-natural amino acids increases the versatility of protein engineering still further. It is expected that such methods will have large impact in the field of materials science, and especially in biomedical materials science, in the future

Individualisation of Mycophenolate Mofetil Therapy: Explaining variability in mycophenolic acid pharmacokinetics and introducing therapeutic

The prodrug mycophenolate mofetil contains the active compound mycophenolic acid (MPA), which has immunosuppressive properties. It is used to prevent acute rejection after solid organ transplantation. In renal transplantation, the dose recommendation for mycophenolate mofetil is 1000 mg twice daily for adult patients. This fixed dose strategy for mycophenolate mofetil is remarkable in the field of transplantation as most other immunosuppressive drugs are used in an individualised dose, often based on drug concentration measurements. During the use of mycophenolate mofetil in the past ten years, data have become available which provide four reasons to question the justification of a fixed mycophenolate mofetil dose. The first reason is the existence of a concentration-effect relationship: the risk for acute rejection is lower when exposure to MPA is higher. This has led to the adoption of a target exposure range for MPA area-under-the-curve (AUC0-12) values of 30 to 60 mg*h/L. The second reason is the large between-patient variability in MPA pharmacokinetics, reported to be more than 10-fold for MPA AUC0-12. The third reason is that MPA exposure increases over time after transplantation despite a fixed dose. Finally, exposure to MPA is significantly influenced by the use of several other drugs. The result of these four factors is that with the use of a standard dose of mycophenolate mofetil, an important subset of renal transplant recipients will have MPA exposure outside the target range, and may therefore be at risk for acute rejection or toxicity. Individualisation of the mycophenolate mofetil dose is likely to improve exposure to MPA and may optimise clinical outcome. The aim of this thesis was to develop recommendations about when and how to individualise the mycophenolate mofetil dose. Two hypotheses in this regard were addressed, formulated in c

Standaardnederlands, de sleutel tot integratie in Vlaanderen? Over de uitdagingen van niet-standaardtaal voor NT2-leerders in Vlaanderen

Flanders strongly encourages newcomers to take language courses to learn the official language, on the assumption that certified competence in Dutch will enhance integration. However, in Dutch L2-education the focus lies on Standard Dutch, and this contrasts to everyday spoken encounters in Flanders, where Standard Dutch is rarely used. Despite the perceived importance of learning the official language of the host society, the attested contrast between policy and language reality has up to now not been given much attention. In this publication we go into the results of a linguistic-ethnographic study conducted with language learners living in East-Flanders. We show that non-standard Dutch used by L1-speakers of Dutch sometimes causes comprehension difficulties in their interactions with the language learners. Based on interactional and interview data, we reflect on the question if L2-education should pay more attention to non-standard Dutch in class

Territorial Factors in a Globalised Art World?

In comparison to other disciplines of high culture, the visual arts seem to be the most suitable to internationalise (cf. Crane, 1992; Janssen, Kuipers & Verboord, 2008). The global diffusion of visual art works, for example, is far less complex than that of the performing arts, which entails an impressive transportation operation of both goods – instruments, sets, costumes – and the actors involved, such as orchestra musicians, theatre company actors or dancers. The global distribution of literature, meanwhile, is easier to achieve than is the case for the visual arts, yet language obstacles manifestly hinder its internationalisation (Heilbron, 1999; Janssen, 2009; Sapiro, 2010). Indeed, the only way to overcome such hurdles is when the author provides a translation of the work, or when the reader learns the foreign language in which it is written (De Swaan, 2001). This is similar in the case of theatre plays or the cinema (Hofstede, 2000). On the other hand, the visual language is deemed to be universal and is unhindered by these issues. In short, while the internationalisation of other domains demands different kinds of investment, the visual arts seem to have the best conditions for artists to conquer the world

Bio-inks for 3D bioprinting : recent advances and future prospects

In the last decade, interest in the field of three-dimensional (3D) bioprinting has increased enormously. 3D bioprinting combines the fields of developmental biology, stem cells, and computer and materials science to create complex bio-hybrid structures for various applications. It is able to precisely place different cell types, biomaterials and biomolecules together in a predefined position to generate printed composite architectures. In the field of tissue engineering, 3D bioprinting has allowed the study of tissues and organs on a new level. In clinical applications, new models have been generated to study disease pathogenesis. One of the most important components of 3D bio-printing is the bio-ink, which is a mixture of cells, biomaterials and bioactive molecules that creates the printed article. This review describes all the currently used bio-printing inks, including polymeric hydrogels, polymer bead microcarriers, cell aggregates and extracellular matrix proteins. Amongst the polymeric components in bio-inks are: natural polymers including gelatin, hyaluronic acid, silk proteins and elastin; and synthetic polymers including amphiphilic block copolymers, PEG, poly(PNIPAAM) and polyphosphazenes. Furthermore, photocrosslinkable and thermoresponsive materials are described. To provide readers with an understanding of the context, the review also contains an overview of current bio-printing techniques and finishes with a summary of bio-printing applications

Over de tweestrijd tussen taalnorm en taalrealiteit : hoe NT2-leerkrachten uit een Gentse school worstelen met niet-standaardtalige variatie in het NT2-onderwijs

Flanders, the Dutch-speaking part of Belgium, shows a strong presence of non-standard language varieties, such as tussentaal (lit. ‘in-between language’) or regional dialects. However, Dutch as a second language (L2) education in Flanders mainly focuses on (Belgian) Standard Dutch, the official language variety in Flanders. Newcomers settling in Flanders seem to experience a large gap between the standard language advocated in policy and education and the multitude of non-standard language varieties they encounter in daily interactions. L2 teachers in Flanders are thus dealing with students who often struggle to communicate with L1 speakers of Dutch. In this empirical study, we address this issue by probing the opinions of L2 teachers: do theybelieve tussentaal and dialects form a communication threshold for their students? To what extent do they focus on non-standard language during their lessons, and what reservations or doubts do they possibly have about teaching non-standard language in the classroom? To answer these questions, we draw on fieldwork conducted in the East Flemish city of Ghent

Incorporating Mythic and Interpretive Analysis in the Investigation of Hearing Loss on the Family Farm

Despite knowing about the dangers of hearing loss, farmers typically choose not to protect their hearing. Examining the myth of farm life, this study aims to discern whether rhetorical myths influence farmers\u27 decisions to wear hearing protection. Researchers conducted 40 interviews with farmers regarding farm life and hearing loss. Results suggest that farmers typically do not use hearing protection; their answers reflect the myths of sacrifice and safety. Analysis demonstrates that knowledge of the relationship between myth and practice should impact future attempts to change farmers\u27 behavior

Kriteria Visibilitas Hilal Rukyatul Hilal Indonesia (Rhi) (Konsep, Kriteria, dan Implementasi)

Telah dilaksanakan observasi hilal dan hilal tua selama periode Zulhijjah 1427–Zulhijjah 1430 H (Januari 2007–Desember 2009) oleh jejaring titik observasi Rukyatul Hilal Indonesia (RHI) yang merentang dari lintang 5° LU hingga 31° LS, dengan ataupun tanpa bantuan alat bantu optik. Observasi menghasilkan 174 data visibilitas yang terdiri dari 107 visibilitas positif dan 67 visibilitas negatif. Analisis korelasi linier Lag dengan Best Time Bulan menghasilkan definisi baru tentang hilal, yaitu Bulan pasca konjungsi yang memiliki Lag ≤ 24 menit hingga Lag ≤ 40 menit saat Matahari terbenam. Hubungan Best Time dan Lag memenuhi persamaan linear Yallop hanya untuk Lag ≤ 40 menit. Analisis korelasi aD dan DAz dengan metode least–square menghasilkan persamaan kriteria RHI aD ≥ 0,099 DAz2–1,490 DAz + 10,382 yang bentuknya hampir sama dengan kriteria LAPAN, namun sangat berbeda dibanding kriteria Fotheringham–Maunder maupun Bruin. Analisa komparatif menyimpulkan asumsi yang dipergunakan “kriteria” Imkanur Rukyat versi MABIMS dan konsep wujudul hilal tidak terbukti. Sebaliknya, terdapat kesesuaian antara hasil observasi dengan kriteria Odeh

Алгоритм функціонування системи захисту

Детально розглянуті алгоритми виявлення атак у середовищі мереж зв'язку.In detail the algorithms of exposure of attacks are considered in the environment of communication network