Nutritional effects by beta-carotene in liver in males and females of control wildtype mice versus BCMO knockout mice

Molecular mechanisms triggered by high dietary beta-carotene (BC) intake in liver are largely unknown. We performed microarray gene expression analysis on liver tissue of BC supplemented beta-carotene 15,150-monooxygenase 1 knockout (Bcmo1-/-) mice, which are—like humans—able to accumulate BC. This was compared with litter mates being wild-type (Bcmo1+/+) mice, and we analysed both males and females, as we previously showed that in lung tissue we observed opposite gene regulation between males and females

Nutritional effects by beta-carotene in liver in males and females of control wildtype mice versus BCMO knockout mice

Molecular mechanisms triggered by high dietary beta-carotene (BC) intake in liver are largely unknown. We performed microarray gene expression analysis on liver tissue of BC supplemented beta-carotene 15,150-monooxygenase 1 knockout (Bcmo1-/-) mice, which are—like humans—able to accumulate BC. This was compared with litter mates being wild-type (Bcmo1+/+) mice, and we analysed both males and females, as we previously showed that in lung tissue we observed opposite gene regulation between males and females

Assessing the effect of water harvesting techniques on event-based hydrological responses and sediment yield at a catchment scale in northern Ethiopia using the Limburg Soil Erosion Model (LISEM)

Runoff and sediment yield in semi-arid catchments are highly influenced by infrequent but very heavy rains. These events occur over short temporal scales, so runoff and sediment transport can only be understood using an event-based analysis. We applied a hydrological and soil-erosion model, LISEM, to the Gule catchment (~ 12 km2) in northern Ethiopia. The objectives of the study were: (a) to evaluate the performance of LISEM in describing event-based hydrological processes and sediment yield in a catchment under the influence of different water harvesting techniques (WHTs), and (b) to study the effect of the WHTs on catchment-scale event-based runoff and sediment yield. The model performed satisfactorily (NSE > 0.5) for most of the events when discharge was calibrated at the main outlet (Gule) and at a sub-outlet (Misbar). Runoff coefficients for the Gule catchment and Misbar sub-catchment were expectedly low due to the implementation of WHTs, which can store runoff from the rains and increase infiltration into the soil. Simulated and measured sediment yields were of similar orders of magnitude. LISEM generally overestimated sediment yield compared to the measurements. The poor performance of LISEM in predicting sediment yield could be attributed to the uncertainty of several factors controlling soil erosion and the inadequacy of LISEM in describing soil erosion on steep slopes. Catchment-scale model simulations indicated that runoff and sediment yield could be effectively reduced by implementing WHTs. The model estimated 41 and 61% reductions in runoff and sediment yield at the Gule outlet, respectively. Similarly, runoff and sediment yield at the Misbar sub-outlet were reduced by 45 and 48%, respectively. LISEM can thus be used to simulate the effects of different existing or new WHTs on catchment hydrology and sediment yield. The results of scenario predictions could be useful for land-use planners who intend to implement different measures of catchment management

Bronchoconstriction and airway hyperresponsiveness after ovalbumin inhalation in sensitized mice

To investigate the mechanisms underlying airway hyperresponsiveness a murine model was developed with several important characteristics of human allergic asthma. Mice were intraperitoneally sensitized with ovalbumin and after 4 weeks challenge via an ovalbumin aerosol. After aerosol, lung function was evaluated with a non-invasive forced oscillation technique. The amount of mucosal exudation into the airway lumen and the presence of mast cell degranulation was determined. Tracheal responsiveness was measured at several time points after challenge. At these time points also bronchoalveolar lavage and histology were performed. Sensitization induced high antigen-specific IgE levels in serum. Inhalation of ovalbumin in sensitized mice induced an immediate but no late bronchoconstrictive response. During this immediate phase, respiratory resistance was increased (54%). Within the first hour after ovalbumin inhalation increased mucosal exudation and mast cell degranulation were observed. At 12 and 24 h after ovalbumin challenge, mice showed tracheal hyperresponsiveness (29% and 34%, respectively). However, no apparent inflammation was found in the lungs or bronchoalveolar lavage. From these results it can be concluded that hyperresponsiveness can develop via mechanisms independent of an inflammatory infiltrate. Since mast cell degranulation occurred after ovalbumin exposure, we hypothesize that mast cells are involved in the induction of airway hyperresponsiveness in this model

Green catalysis by nanoparticulate catalysts developed for flow processing? case study of glucose hydrogenation

Heterogeneous catalysis, flow chemistry, continuous processing, green solvents, catalyst immobilization and recycling are some of the most relevant, emerging key technologies to achieve green synthesis. However, a quantification of potential effects on a case to case level is required to provide a profound answer, whether they can lead to a superior process compared to the industrial standard. To do so, holistic environmental assessment approaches are very useful tools providing insights and decision support during the process development phase. Herein, novel heterogeneous nanoparticulate ruthenium catalysts immobilized on hyperbranched polystyrene (HPS) and nitrogen-doped carbon nanotubes (NCNT) were investigated with respect to their potential environmental impacts and improvements if utilized in an industrially highly relevant process, namely glucose hydrogenation to sorbitol. The results of a comparative Life Cycle Assessment of the alternative catalytic systems under consideration of RANEY® nickel as benchmark catalyst revealed that in particular Ru nanoparticles on porous HPS beads processed under flow-chemistry conditions have the potential to improve the greenness of the overall synthesis, but the concentration of glucose in the reaction mixture is in fact the most influential parameter