Intestinal epithelial responses to Salmonella enterica serovar Enteritidis: Effects on intestinal permeability and ion transport

Salmonella infection of chickens that leads to potential human foodborne salmonellosis continues to be a major concern. Chickens serve as carriers but, in contrast to humans, rarely show any clinical signs including diarrhea. The present investigations aimed to elucidate whether the absence of diarrhea during acute Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) infection may be linked to specific changes in the electrophysiological properties of the chicken gut. Immediately after slaughter, intestinal pieces of the mid-jejunum and cecum of either commercial broiler or specific pathogen-free (SPF) chickens were mounted in Ussing chambers in 2 separate experimental series. Living Salmonella Enteritidis (3 × 109) or Salmonella Enteritidis endotoxin (20 mg/L), or both, were added to the mucosal side for 1 h. In both experimental series, the Salmonella infection decreased the trans-epithelial ion conductance Gt (P < 0.05). In the jejunum of SPF chickens, there was also a marked decrease in net charge transfer across the epithelium, evidenced by decreased short-circuit current (Isc, P < 0.05). Interestingly, the mucosal application of Salmonella endotoxin to the epithelial preparations from jejunum and cecum of SPF chicken had an effect similar to living bacteria. However, the endotoxin had no additional effect on the intestinal function in the presence of bacteria. The decreasing effect of Salmonella and or its endotoxin on Gt could be partly reversed by serosal addition of histamine. To our knowledge, this is the first study to address the functional response of native intestinal epithelium of chicken to an in vitro Salmonella infection. For the first time, it can be reported that intestinal ion permeability of chicken decreases acutely by the presence of Salmonella. This type of response could counteract ion and fluid secretion and may thus, at least in part, explain why chickens do not develop overt diarrhea after Salmonella infection

An Isotopic analysis of the hydrology and riparian vegetation water sources on Bishop Creek

Five power generation plants along an eleven kilometer stretch divert Bishop Creek water for hydro-electric power. Stream diversion may be adversely affecting the riparian vegetation. Stable isotopic analysis is employed to determine surface water/ground-water interactions along the creek. surface water originates primarily from three headwater lakes. Discharge into Bishop Creek below the headwaters is primarily derived from ground water. The average δD and δ18O values are significantly different for surface water and ground water that an isotopic analysis can delineate between these two components of flow. Therefore isotopic shifts along the creek can determine gaining reaches. In addition, by knowing the isotopic signatures of various waters in the watershed, it may be possible to examine tree waters to determine their water source(s)

Campylobacter jejuni colonization promotes the translocation of Escherichia coli to extra-intestinal organs and disturbs the short-chain fatty acids profiles in the chicken gut

For a long time Campylobacter was only considered as a commensal microorganism in avian hosts restricted to the ceca, without any pathogenic features. The precise reasons for the symptomless chicken carriers are still unknown, but investigations of the gastrointestinal ecology of broiler chickens may improve our understanding of the microbial interactions with the host. Therefore, the current studies were conducted to investigate the effects of Campylobacter jejuni colonization on Escherichia coli translocation and on the metabolic end products (short-chain fatty acids, SCFAs). Following oral infection of 14 day old broiler chickens with 1 × 108 CFU of Campylobacter jejuni NCTC 12744 in two independent animal trials, it was found that C. jejuni heavily colonized the intestine and disseminate to extra-intestinal organs. Moreover, in both animal trials, the findings revealed that C. jejuni promoted the translocation of E. coli with a higher number encountered in the spleen and liver at 14 days post infection (dpi). In addition, Campylobacter affected the microbial fermentation in the gastrointestinal tract of broilers by reducing the amount of propionate, isovalerate, and isobutyrate in the cecal digesta of the infected birds at 2 dpi and, at 7 and 14 dpi, butyrate, isobutyrate, and isovalerate were also decreased. However, in the jejunum, the C. jejuni infection lowered only butyrate concentrations at 14 dpi. These data indicated that C. jejuni may utilize SCFAs as carbon sources to promote its colonization in the chicken gut, suggesting that Campylobacter cannot only alter gut colonization dynamics but might also influence physiological processes due to altered microbial metabolite profiles. Finally, the results demonstrated that C. jejuni can cross the intestinal epithelial barrier and facilitates the translocation of Campylobacter itself as well as of other enteric microorganisms such as E. coli to extra-intestinal organs of infected birds. Altogether, our findings suggest that the Campylobacter carrier state in chicken is characterised by multiple changes in the intestinal barrier function, which supports multiplication and survival within the host

Calculation of compressible flow about three-dimensional inlets with auxiliary inlets, slats and vanes by means of a panel method

An efficient and user oriented method was constructed for calculating flow in and about complex inlet configurations. Efficiency is attained by: (1) the use of a panel method; (2) a technique of superposition for obtaining solutions at any inlet operating condition; and (3) employment of an advanced matrix iteration technique for solving large full systems of equations, including the nonlinear equations for the Kutta condition. User concerns are addressed by the provision of several novel graphical output options that yield a more complete comprehension of the flowfield than was possible previously

Recent transonic unsteady pressure measurements at the NASA Langley Research Center

Four semispan wing model configurations were studied in the Transonic Dynamics Tunnel (TDT). The first model had a clipped delta planform with a circular arc airfoil, the second model had a high aspect ratio planform with a supercritical airfoil, the third model has a rectangular planform with a supercritical airfoil and the fourth model had a high aspect ratio planform with a supercritical airfoil. To generate unsteady flow, the first and third models were equipped with pitch oscillation mechanisms and the first, second and fourth models were equipped with control surface oscillation mechanisms. The fourth model was similar in planform and airfoil shape to the second model, but it is the only one of the four models that has an elastic wing structure. The unsteady pressure studies of the four models are described and some typical results for each model are presented. Comparison of selected experimental data with analytical results also are included

Chemical differentiation on one-plate planets: Predictions and geologic observations for Venus

Recent studies have examined the partial melting of planetary interiors on one-plate planets and the implications for the formation and evolution of basaltic crust and the complementary residual mantle layer. In contrast to the Earth, where the crust and residual layer move laterally and are returned to the interior following subduction, one-plate planets such as Venus are characterized by vertical accretion of the crust and residual layer. The residual mantle layer is depleted and compositionally buoyant, being less dense than undepleted mantle due to its reduced Fe/Mg and dense Al-bearing minerals; its melting temperature is also increased. As the crust and depleted mantle layer grow vertically during the thermal evolution of the planet, several stages develop. As a step in the investigation and testing of these theoretical treatments of crustal development on Venus, we investigate the predictions deriving from two of these stages (a stable thick crust and depleted layer, and a thick unstable depleted layer) and compare these to geologic and geophysical observations, speculating on how these might be interpreted in the context of the vertical crustal accretion models. In each case, we conclude with an outline of further tests and observations of these models

A Scanning Electron Microscopy Study of Taxus Leaves as Related to Taxonomy

Scanning electron microscopy, when applied to the surfaces of the needles of Taxus spp. (yew) revealed features that appear useful in the taxonomy of this tree species which yields the important anticancer drug, taxol. For instance, all of the four North American species have 3-5 rows of stomata on one-half of the abaxial leaf surface, whereas all of the others, including those from Europe and Asia, have 7-10 rows of stomata. The appearance of individual or fused papilliform epidermal cells and their arrangement on the leaf surface also is a feature that varies between species. Patterns of wax formation appeared on all species of yew examined but none could characteristically be assigned to a given species. Wax pattern variation was dependent upon age, environment, and probably to some extent, species differences. This study may provide some additional useful and reliable indicators in Taxus taxonomy