Mass Spectrometry in the Elucidation of the Glycoproteome of Bacterial Pathogens

Presently some three hundred post-translational modifications are known to occur in bacteria in vivo. Many of these modifications play critical roles in the regulation of proteins and control key biological processes. One of the most predominant modifications, N- and O-glycosylations are now known to be present in bacteria (and archaea) although they were long believed to be limited to eukaryotes. In a number of human pathogens these glycans have been found attached to the surfaces of pilin, flagellin and other surface and secreted proteins where it has been demonstrated that they play a role in the virulence of these bacteria. Mass spectrometry characterization of these glycosylation events has been the enabling key technology for these findings. This review will look at the use of mass spectrometry as a key technology for the detection and mapping of these modifications within microorganisms, with particular reference to the human pathogens, Campylobacter jejuni and Mycobacterium tuberculosis. The overall aim of this review will be to give a basic understanding of the current ‘state-of-the-art’ of the key techniques, principles and technologies, including bioinformatics tools, involved in the analysis of the glycosylation modifications

A geometry package for generation of input data for a three-dimensional potential-flow program

The preparation of geometric data for input to three-dimensional potential flow programs was automated and simplified by a geometry package incorporated into the NASA Langley version of the 3-D lifting potential flow program. Input to the computer program for the geometry package consists of a very sparse set of coordinate data, often with an order of magnitude of fewer points than required for the actual potential flow calculations. Isolated components, such as wings, fuselages, etc. are paneled automatically, using one of several possible element distribution algorithms. Curves of intersection between components are calculated, using a hybrid curve-fit/surface-fit approach. Intersecting components are repaneled so that adjacent elements on either side of the intersection curves line up in a satisfactory manner for the potential-flow calculations. Many cases may be run completely (from input, through the geometry package, and through the flow calculations) without interruption. Use of the package significantly reduces the time and expense involved in making three-dimensional potential flow calculations

Two-state shear diagrams for complex fluids in shear flow

The possible "phase diagrams'' for shear-induced phase transitions between two phases are collected. We consider shear-thickening and shear-thinning fluids, under conditions of both common strain rate and common stress in the two phases, and present the four fundamental shear stress vs. strain rate curves and discuss their concentration dependence. We outline how to construct more complicated phase diagrams, discuss in which class various experimental systems fall, and sketch how to reconstruct the phase diagrams from rheological measurements

Current activities at IITRI on high- temperature protective coatings

Heat resistant protective coatings for use in liquid propellant rocket engine

Improved solution for potential flow about arbitrary axisymmetric bodies by the use of a higher-order surface source method. Part 1. Theory and results

The surface-source method of calculating potential flow is improved by refining the underlying numerical analysis. The analysis uses parabolic elements and linearly-varying source density. The result is a large increase in computing speed and accuracy. The theory is described, and the effectiveness of the modification is illustrated