Reconstruction of the space station before and proposal roundabout in the village Týniště nad Orlicí

Předmětem bakalářské práce je rekonstrukce přednádraží a návrh okružní křižovatky v obci Týniště nad Orlicí. Bude navržena odlišná varianta stávající rekonstrukce.The subject of this thesis is reconstruction of the space station before and proposal roundabout in the village Týniště nad Orlicí. It will be designed different variations of existing reconstruction.

III/29920 Kuks - Stanovice, road reconstruction

Předmětem diplomové práce je rekonstrukce silnice III/29920 v obci kuks a Stanovice, od křižovatky se silnicí I/37 až po most e.č. 29920-1, v délce 1,5km.The subject of this thesis is the reconstruction of the road III/29920 in the village Kuks and Stanovice, from the junction with the road I/37 to the bridge e.č. 29920-1, 1.5 km in length.

Structural biology of receptor NKp44 and its ligand PCNA

Součástí imunitního systému nejen člověka jsou tzv. NK buňky (z anglického natural killer cells). Úkolem těchto buněk, které jsou složkou nespecifické imunity, je vyvolat apoptózu u buněk tělu vlastních, které jsou hrozbou pro organismus (například buňky napadené viry či rakovinné buňky). K rozpoznání cílových buněk slouží NK buňkám povrchové receptory. Receptorů je dnes známa celá řada a jejich dělení vyplývá například z podobnosti jejich struktur, nebo povahy signálu, který zprostředkovávají NK buňkám. Podle toho rozlišujeme receptory aktivační a inhibiční. Inhibiční receptory potlačují NK buněčnou odpověď, zatímco receptory aktivační tuto odpověď vyvolávají. Během kontaktu NK buňky s jinou buňkou je výsledné chování NK buňky vždy výsledkem určité rovnováhy odpovědí receptorů aktivačních i inhibičních. Receptor NKp44 je imunoglobulinový receptor. Tento receptor je mezi ostatními receptory velmi ojedinělý z mnoha hledisek, například i proto, že je spojen jak s aktivačním, tak inhibičním motivem. Ligandem tohoto receptoru je jaderný antigen proliferujících buněk (PCNA). PCNA je svorkový protein důležitý mimo jiné během replikace DNA, při které ukotvuje ostatní proteiny replizómu. Tato práce je zaměřena na rekombinantní přípravu receptoru NKp44 (protein PCNA byl již připraven dříve) a studium...Natural killer cells (NK cells) are part of the immune system in human and other mammals. The task of these cells, which belong to the non-specific immunity, is to induce apoptosis in other cells of the body that may represent a threat for the body (i.e., tumour or virally infected cells). NK cells have a variety of surface receptors to recognize their target cells. A number of receptors are well-known today and they may be divided into groups based, e.g., on their structural similarities or on the type of signal which these receptors present to NK cells. Accordingly, we distinguish activation and inhibitory receptors. Inhibitory receptors inhibit NK cell response, while activation receptors elicit this response. During NK cell contact with another cell, the resulting NK cell behaviour is always the result of a certain balance of activation and inhibitory receptor responses. The NKp44 receptor is an immunoglobulin-like receptor. This receptor is very unique among other receptors in many respects, for example because it is associated with both activation and inhibitory motif. The ligand of this receptor is a proliferating cell nuclear antigen (PCNA). PCNA is a clamp protein important, inter alia, during DNA replication, in which it anchors other replisome proteins. This work is focused on...Department of BiochemistryKatedra biochemiePřírodovědecká fakultaFaculty of Scienc

Application of FMEA method in engineering company

Bakalářská práce se zaměřuje na využití kvalitativní metody FMEA v konkrétním strojírenském podniku a na konkrétním procesu, ve kterém dochází k inovaci a na základě aplikace metody je cílem předejít možné budoucí chybovosti.The bachelor´s thesis focuses on the application of FMEA method in concrete engineering company on concrete process, which is going to be innovated and with using FMEA method is goal to prevent possible future failure.

The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells

Sodium hyaluronate (HA) was associated with dopamine (DPA) and introduced as a coating for maghemite (γ-Fe2O3) nanoparticles obtained by the coprecipitation of iron(II) and iron(III) chlorides and oxidation with sodium hypochlorite. The effects of the DPA anchorage of HA on the γ-Fe2O3 surface on the physicochemical properties of the resulting colloids were investigated. Nanoparticles coated at three different DPA-HA/γ-Fe2O3 and DPA/HA ratios were chosen for experiments with rat bone marrow mesenchymal stem cells and human chondrocytes. The nanoparticles were internalized into rat bone marrow mesenchymal stem cells via endocytosis as confirmed by Prussian Blue staining. The efficiency of mesenchymal stem cell labeling was analyzed. From among the investigated samples, efficient cell labeling was achieved by using DPA-HA-γ-Fe2O3 nanoparticles with DPA-HA/γ-Fe2O3 = 0.45 (weight/ weight) and DPA/HA = 0.038 (weight/weight) ratios. The particles were used as a contrast agent in magnetic resonance imaging for the labeling and visualization of cells

Forward pi^0 Production and Associated Transverse Energy Flow in Deep-Inelastic Scattering at HERA

Deep-inelastic positron-proton interactions at low values of Bjorken-x down to x \approx 4.10^-5 which give rise to high transverse momentum pi^0 mesons are studied with the H1 experiment at HERA. The inclusive cross section for pi^0 mesons produced at small angles with respect to the proton remnant (the forward region) is presented as a function of the transverse momentum and energy of the pi^0 and of the four-momentum transfer Q^2 and Bjorken-x. Measurements are also presented of the transverse energy flow in events containing a forward pi^0 meson. Hadronic final state calculations based on QCD models implementing different parton evolution schemes are confronted with the data.Comment: 27 pages, 8 figures and 3 table

Deep-Inelastic Inclusive ep Scattering at Low x and a Determination of alpha_s

A precise measurement of the inclusive deep-inelastic e^+p scattering cross section is reported in the kinematic range 1.5<= Q^2 <=150 GeV^2 and 3*10^(-5)<= x <=0.2. The data were recorded with the H1 detector at HERA in 1996 and 1997, and correspond to an integrated luminosity of 20 pb^(-1). The double differential cross section, from which the proton structure function F_2(x,Q^2) and the longitudinal structure function F_L(x,Q^2) are extracted, is measured with typically 1% statistical and 3% systematic uncertainties. The measured partial derivative (dF_2(x,Q^2)/dln Q^2)_x is observed to rise continuously towards small x for fixed Q^2. The cross section data are combined with published H1 measurements at high Q^2 for a next-to-leading order DGLAP QCD analysis.The H1 data determine the gluon momentum distribution in the range 3*10^(-4)<= x <=0.1 to within an experimental accuracy of about 3% for Q^2 =20 GeV^2. A fit of the H1 measurements and the mu p data of the BCDMS collaboration allows the strong coupling constant alpha_s and the gluon distribution to be simultaneously determined. A value of alpha _s(M_Z^2)=0.1150+-0.0017 (exp) +0.0009-0.0005 (model) is obtained in NLO, with an additional theoretical uncertainty of about +-0.005, mainly due to the uncertainty of the renormalisation scale.Comment: 68 pages, 24 figures and 18 table

Searches at HERA for Squarks in R-Parity Violating Supersymmetry

A search for squarks in R-parity violating supersymmetry is performed in e^+p collisions at HERA at a centre of mass energy of 300 GeV, using H1 data corresponding to an integrated luminosity of 37 pb^(-1). The direct production of single squarks of any generation in positron-quark fusion via a Yukawa coupling lambda' is considered, taking into account R-parity violating and conserving decays of the squarks. No significant deviation from the Standard Model expectation is found. The results are interpreted in terms of constraints within the Minimal Supersymmetric Standard Model (MSSM), the constrained MSSM and the minimal Supergravity model, and their sensitivity to the model parameters is studied in detail. For a Yukawa coupling of electromagnetic strength, squark masses below 260 GeV are excluded at 95% confidence level in a large part of the parameter space. For a 100 times smaller coupling strength masses up to 182 GeV are excluded.Comment: 32 pages, 14 figures, 3 table