2,773 research outputs found

    Nitrogen fertilization increased grass litter decomposition in a tropical agroforestry system.

    Get PDF
    Litter decomposition and livestock excreta are two important sources for replenishing nutrients in the soil of the pastures, and their decomposition rates are afected by their quality, management practices, forage productivity, and biotic and abiotic factors. The objective of this research was to assess the efects of escalating levels of N fertilization (0, 100, 200, and 400 kg N ha−1 yr−1) on litter and fecal decomposition in an agroforestry system comprising palisadegrass [Urochloa brizantha (Hochst. Ex A.Rich.) Stapf. cv. Marandu] intercropped with hybrid eucalyptus trees [Eucalyptus urophylla×Eucalyptus tereticornis], in a two-year feld trial. The experiment was set in a randomized complete block design with four treatments and three repetitions. Litter (0, 4, 8, 16, 32, 64, 128, and 256 days) and cattle excrement samples (0, 4, 8, 16, 32, 64, and 128 days) were incubated on the ground. For forage litter samples, the interaction between N fertilization×year was observed for the decomposition rate (k) of DM (P=0.0014) and OM (P=0.0094). The greatest litter OM disappearance was observed at 400 kg N fertilization ha−1 year−1 (651 g kg−1 DM at 256 days). The interaction between nitrogen fertilizer rate×incubation time, or the isolated efect of the treatment was not observed on fecal decomposition (P>0.05). Higher levels of N fertilization associated with the rainy period resulted in faster decomposition of palisadegrass litter, however, it did not show to have a strong infuence on the excreta decomposition in this agroforestry system

    The structure of Chariklo's rings from stellar occultations

    Get PDF
    Two narrow and dense rings (called C1R and C2R) were discovered around the Centaur object (10199) Chariklo during a stellar occultation observed on 2013 June 3. Following this discovery, we planned observations of several occultations by Chariklo's system in order to better characterize the physical properties of the ring and main body. Here, we use 12 successful occulations by Chariklo observed between 2014 and 2016. They provide ring profiles (physical width, opacity, edge structure) and constraints on the radii and pole position. Our new observations are currently consistent with the circular ring solution and pole position, to within the ±3.3\pm 3.3 km formal uncertainty for the ring radii derived by Braga-Ribas et al. The six resolved C1R profiles reveal significant width variations from ∌5\sim 5 to 7.5 km. The width of the fainter ring C2R is less constrained, and may vary between 0.1 and 1 km. The inner and outer edges of C1R are consistent with infinitely sharp boundaries, with typical upper limits of one kilometer for the transition zone between the ring and empty space. No constraint on the sharpness of C2R's edges is available. A 1σ\sigma upper limit of ∌20\sim 20 m is derived for the equivalent width of narrow (physical width <4 km) rings up to distances of 12,000 km, counted in the ring plane

    Functional Characterization of an Aspergillus fumigatus Calcium Transporter (PmcA) that Is Essential for Fungal Infection

    Get PDF
    Aspergillus fumigatus is a primary and opportunistic pathogen, as well as a major allergen, of mammals. The Ca+2-calcineurin pathway affects virulence, morphogenesis and antifungal drug action in A. fumigatus. Here, we investigated three components of the A. fumigatus Ca+2-calcineurin pathway, pmcA,-B, and -C, which encode calcium transporters. We demonstrated that CrzA can directly control the mRNA accumulation of the pmcA-C genes by binding to their promoter regions. CrzA-binding experiments suggested that the 5â€Č-CACAGCCAC-3â€Č and 5â€Č-CCCTGCCCC-3â€Č sequences upstream of pmcA and pmcC genes, respectively, are possible calcineurin-dependent response elements (CDREs)-like consensus motifs. Null mutants were constructed for pmcA and -B and a conditional mutant for pmcC demonstrating pmcC is an essential gene. The ΔpmcA and ΔpmcB mutants were more sensitive to calcium and resistant to manganese and cyclosporin was able to modulate the sensitivity or resistance of these mutants to these salts, supporting the interaction between calcineurin and the function of these transporters. The pmcA-C genes have decreased mRNA abundance into the alveoli in the ΔcalA and ΔcrzA mutant strains. However, only the A. fumigatus ΔpmcA was avirulent in the murine model of invasive pulmonary aspergillosis
    • 

    corecore