Very Rare Complementation between Mitochondria Carrying Different Mitochondrial DNA Mutations Points to Intrinsic Genetic Autonomy of the Organelles in Cultured Human Cells

In the present work, a large scale investigation was done regarding the capacity of cultured human cell lines (carrying in homoplasmic form either the mitochondrial tRNALys A8344G mutation associated with the myoclonic epilepsy and ragged red fiber (MERRF) encephalomyopathy or a frameshift mutation, isolated in vitro, in the gene for the ND4 subunit of NADH dehydrogenase) to undergo transcomplementation of their recessive mitochondrial DNA (mtDNA) mutations after cell fusion. The presence of appropriate nuclear drug resistance markers in the two cell lines allowed measurements of the frequency of cell fusion in glucose-containing medium, non-selective for respiratory capacity, whereas the frequency of transcomplementation of the two mtDNA mutations was determined by growing the same cell fusion mixture in galactose-containing medium, selective for respiratory competence. Transcomplementation of the two mutations was revealed by the re-establishment of normal mitochondrial protein synthesis and respiratory activity and by the relative rates synthesis of two isoforms of the ND3 subunit of NADH dehydrogenase. The results of several experiments showed a cell fusion frequency between 1.4 and 3.4% and an absolute transcomplementation frequency that varied between 1.2 × 10^-5 and 5.5 × 10^-4. Thus, only 0.3-1.6% of the fusion products exhibited transcomplementation of the two mutations. These rare transcomplementing clones were very sluggish in developing, grew very slowly thereafter, and showed a substantial rate of cell death (22-28%). The present results strongly support the conclusion that the capacity of mitochondria to fuse and mix their contents is not a general intrinsic property of these organelles in mammalian cells, although it may become activated in some developmental or physiological situations

Representaciones gráficas. Una estrategia didáctica en el planteamiento y resolución de problemas de probabilidad

Este artículo es el reporte del taller sobre técnicas y estrategias gráficas para la resolución de problemas de probabilidad, llevado a cabo en el Relme 25 celebrado en la Cd. de Camaguey, Cuba, bajo objetivos didácticos que abordan la enseñanza aprendizaje de la probabilidad en el nivel superior. Para ello se seleccionaron y presentaron para su análisis diversos tipos de problemas con el fin de presentar las técnicas apropiadas para su solución. Se contó con la participación de profesores y estudiantes en la construcción colectiva de los significados, a partir de una situación didáctica representada por el planteamiento de problemas de probabilidad que requieren diferentes estrategias en la búsqueda de soluciones. Siguiendo recomendaciones de la Teoría de situaciones didácticas de Brousseau (1997)

Beneficios economicos y financieros de la Ley 19.983 que regula la transferencia y otorga merito ejecutivo a copia de la Factura.

50 p.El tema de esta investigación, está en estrecha relación con dos ramas del derecho, como lo son el Derecho Comercial y el Derecho Económico, y, apunta específicamente a “Los Títulos de Crédito”, su regulación y su importancia para el mercado financiero y el desarrollo de las operaciones comerciales que se desarrollan en nuestra economía. Los objetivos generales de esta investigación, consisten esencialmente en describir y analizar la nueva regulación jurídica a que serán sometidas las facturas comerciales, en cuanto documentos que contienen un crédito personal. Asimismo se tratará de explicar los beneficios económicos y financieros que traerá consigo la aplicación de esta nueva ley, 19.983, en importantes sectores de la economía, como lo son la pequeña y mediana empresa y la industria del Factoring

Estrategias didácticas para la construcción de conocimientos estadísticos

En este trabajo de investigación se presenta una guía de aprendizaje construida para utilizar una diversidad de herramientas tecnológicas y matemáticas como parte de una estrategia didáctica, estructurada en función de las necesidades de los estudiantes, donde se cuenta con una variedad de problemas contextuales y factibles, considerando una sociedad en crisis y cuya repercusión se proyecta en el proceso educativo. En la aplicación de ésta, se puede apreciar el hecho de la intencionalidad para utilizar las herramientas, las construcciones de conceptos estadísticos, la motivación del trabajo en equipo y los argumentos presentados por los estudiantes para dar significado a la media aritmética y la noción de variabilidad; como logran darle sentido a la toma de decisiones en forma empírica, basados en los efectos que presenta la inestabilidad de los datos

A Bilevel Approach to Optimal Price-Setting of Time-and-Level-of-Use Tariffs

Time-and-Level-of-Use (TLOU) is a recently proposed pricing policy for energy, extending Time-of-Use with the addition of a capacity that users can book for a given time frame, reducing their expected energy cost if they respect this self-determined capacity limit. We introduce a variant of the TLOU defined in the literature, aligned with the supplier interest to prevent unplanned over-consumption. The optimal price-setting problem of TLOU is defined as a bilevel, bi-objective problem anticipating user choices in the supplier decision. An efficient resolution scheme is developed, based on the specific discrete structure of the lower-level user problem. Computational experiments using consumption distributions estimated from historical data illustrate the effectiveness of the proposed framework

Hamiltonian Forging of a Thermofield Double

We address the variational preparation of Gibbs states as the ground state of a suitably engineered Hamiltonian acting on the doubled Hilbert space. The construction is exact for quadratic fermionic Hamiltonians and gives excellent approximations up to fairly high quartic deformations. We provide a variational circuit whose optimization returns the unitary diagonalizing operator, thus giving access to the whole spectrum. The problem naturally implements the entanglement forging ansatz, allowing the computation of Thermofield Doubles with a higher number of qubits than in competing frameworks.Comment: 11 pages, 6 figure

Relation of surface and underground waters in Chungará and Cotacotani lake districts, northern Chile: an isotopic study.

Lake Chungará and Cotacotani lake districts are surface water bodies located to the northwest of Lauca Basin in the northern Chilean Altiplano. Surface and underground waters show low to moderate saline content and an electrical conductivity in the range of 48.7 to 3090 μS/cm. The lakes are related to a system of aquifers on the nearby volcano flanks and debris avalanche deposits of Parinacota volcano. On the basis of δ18O and δD isotopic composition and its correlation with the chloride content in Chungará and Cotacotani lake, spring, and Chungará river waters it is demonstrated that: a. Lake Chungará's waters show vertical and horizontal homogeneous isotopic composition, which indicates a good mixture of waters in the lake; b. an important part of the Cotacotani lake recharge feeding come from Lake Chungará, that flows laterally as groundwater; c. the groundwater that feeds the springs of the area has its main recharge in the spring-summer (October-March) precipitation. The high content of tritium (3H) measured in spring waters of the area suggested a very recent recharge (last decades) of the aquifer system. The application of lumped parameter models to interpret the water residence time in the aquifer indicates that the piston flow model shows the best fit to the isotopic composition of Chungará-Cotacotani groundwater

Solving Vehicle Routing Problems under Uncertainty and in Dynamic Scenarios: From Simheuristics to Agile Optimization

[EN] Many real-life applications of the vehicle routing problem (VRP) occur in scenarios subject to uncertainty or dynamic conditions. Thus, for instance, traveling times or customers' demands might be better modeled as random variables than as deterministic values. Likewise, traffic conditions could evolve over time, synchronization issues should need to be considered, or a real-time re-optimization of the routing plan can be required as new data become available in a highly dynamic environment. Clearly, different solving approaches are needed to efficiently cope with such a diversity of scenarios. After providing an overview of current trends in VRPs, this paper reviews a set of heuristic-based algorithms that have been designed and employed to solve VRPs with the aforementioned properties. These include simheuristics for stochastic VRPs, learnheuristics and discrete-event heuristics for dynamic VRPs, and agile optimization heuristics for VRPs with real-time requirements.This work was partially funded by the Spanish Ministry of Science, Innovation, and Universities (PID2019-111100RB-C21-C22/AEI/10.13039/501100011033), the SEPIE Erasmus+ Program (2019-I-ES01-KA103-062602), the Barcelona City Council and Fundacio "la Caixa" under the framework of the Barcelona Science Plan 2020-2023 (21S09355-001), and the Generalitat Valenciana (PROMETEO/2021/065).Ammouriova, M.; Herrera, EM.; Neroni, M.; Juan, AA.; Faulin, J. (2023). Solving Vehicle Routing Problems under Uncertainty and in Dynamic Scenarios: From Simheuristics to Agile Optimization. Applied Sciences. 13(1). https://doi.org/10.3390/app1301010113

Occurrence of blastocystis in patients with clostridioides difficile infection

Clostridiodes difficile comprises a public-health threat that has been understudied in Colombia. Hypervirulent strains of C. difficile harbor multiple toxins, can be easily spread, and can have their onset of disease within healthcare facilities (HCFO) and the community (CO). Studies have shown that a disrupted microbiota (e.g., dysbiosis) may allow C. difficile infection (CDI). It has been suggested that dysbiosis prevents colonization by the anaerobic eukaryote Blastocystis, possibly due to an increase in luminal oxygen tension. No study has found co-occurrence of CDI and Blastocystis. Therefore, we aimed to determine the frequencies of C. difficile and Blastocystis infection/colonization in 220 diarrheal fecal samples. Molecular detection by PCR for both microorganisms was performed, with descriptive analyses of four variables (CDI detection, determination of C. difficile toxigenic profiles, Blastocystis detection, and patient site of onset). We demonstrate a significant association between the presence of Blastocystis and CDI, with coinfection found in 61 patients, and show a high frequency of CDI among both HCFO and CO groups. Our results of coinfection frequencies could support hypotheses that suggest Blastocystis can adapt to dysbiosis and oxidative stress. Further, the presence of toxigenic C. difficile occurring outside healthcare facilities shown here raises the alarm for community wide spread. © 2020 by the authors. Licensee MDPI, Basel, Switzerland