First Record of Batrachochytrium dendrobatidis in Physalaemus fernandezae (Anura: Leiuperidae) for Buenos Aires province, Argentina

In Argentina, Batrachochytrium dendrobatidis (Bd) is known from Buenos Aires, Córdoba, Misiones, Neuquén, San Luis, Salta, and Tucumán provinces (Arellano et al. 2009; Barrionuevo and Mangione 2006; Fox et al. 2006; Ghirardi et al. 2009; Gutierrez et al. 2010; Herrera et al. 2005). We provide the first record of Bd infection in a population of the pond-breeding anuran Physalae-mus fernandezae, from Punta Lara Natural Reserve (34.8033 ̊S, 58.0099 ̊W), Ensenada, Buenos Aires province, Argentina.Centro de Investigaciones del Medioambient

Assessing Socio-ecological Systems Using Social Media Data: An Approach for Forested Landscapes in Tierra del Fuego, Argentina

The purpose of this study was to analyse how visitors valued a socio-ecological system through the use of social media data. We gathered YouTube´s videos of Ushuaia city and its surrounding forested landscapes (Tierra del Fuego, Argentina) posted between 2010 and 2020. We used the screen time (seconds) of each video to compare the value of visitors on biophysical, cultural, and biodiversity attributes of the studied socio-ecological system. Each of the visitors registered differently the same attribute (e.g. mountains, forests, signposts, fauna, among others), therefore we assessed the time each visitor spend on any attribute, that was calculated considering the focus and scale through which it was observed. Based on our analyses, we found a diversity of attributes with different valuation data for each visitor. Attributes were classified as biophysical, cultural, and biodiversity variables, and the origin of the visitors (e.g. regions of the world) was also evaluated, with descriptive and multivariate analyses. Results indicated that visitors give more value to biophysical and cultural attributes compared to local biodiversity. These outputs highlight the need to explore and implement alternative methods to assess the socio-ecological values to achieve management objectives, and to include socio-ecological attributes in the study area as key indicators to create better tools and solutions for conservation issues. In this context, we provide a new insight into how visitors can appreciate different socio-ecological values.Fil: Huertas Herrera, Alejandro. Universidad de Magallanes; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Toro Manríquez, Mónica. Universidad de Magallanes; ChileFil: Soler Esteban, Rosina Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Lorenzo, Cristian A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Universidad Nacional de Tierra del Fuego, Antártida e Islas del Atlántico Sur. Instituto de Ciencias Polares, Ambientales y Recursos Naturales; ArgentinaFil: Lencinas, María Vanessa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Martínez Pastur, Guillermo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentin

Impacto de la pizarra luminosa en estudiantes de contaduría pública de estadística inferencial y fundamentos de matemáticas.

Diagnosticar el uso de la pizarra luminosa en cursos cuantitativos.El uso de recursos que inviten al estudiante a estar más comprometido con su proceso educativo puede incrementar su desempeño. El objetivo del estudio es encontrar el nivel de apreciación percibido en el uso de la pizarra luminosa en dos cursos cuantitativos y establecer las diferencias entre el sexo y la situación laboral de los estudiantes participantes. Se utilizó como instrumento una encuesta que diligenciaron los 97 participantes. Se encontró que el 82% de los estudiantes preferían los videos elaborados por el mismo profesor que orientaba la asignatura, el 82% prefirió verle la cara al profesor mientras escribe en el tablero. En las 3 dimensiones, comprensión, compromiso y satisfacción, se obtuvo 97%, 94% y 95% respectivamente en la categoría “Totalmente de acuerdo”. La apreciación total en relación con el sexo no presentó diferencias significativas, al igual que con la situación laboral

Candelilla wax edible coating with Flourensia cernua bioactives to prolong the quality of tomato fruits

The improvement of the postharvest quality of tomato fruits was evaluated using an edible coating functionalized with an Flourensia cernua extract evaluating the antifungal, structural, barrier, and optical properties. The formulation and evaluation of an edible coating and its application on tomato was evaluated using a response surface methodology to determine the ideal concentrations of candelilla wax, whey protein, and glycerol. Edible films showed good barrier properties, with water vapor permeability varying from 0.4350.404 g mm/m2 day kPa. The addition o F. cernua extract showed significant improvement in the transparency of films. The edible coating applied to tomato reduced weight and firmness loss. The sensory evaluation proved that the product obtained is acceptable for consumers. The edible coating added with F. cernua extract was the most effective in inhibiting the growth of pathogenic fungi and the visual appearance at the end of storage confirmed the beneficial effect of the edible coating.(undefined)info:eu-repo/semantics/publishedVersio

Metodologías de Inclusión escolar en el curso 6° básico B de la Escuela Carlos Spano de Talca

70 p.Este trabajo de investigación se enmarca en el ámbito educativo de una escuela perteneciente a la Región del Maule, Chile. Luego de la realización de un análisis FODA en la Escuela Carlos Spano de Talca detectamos un alto porcentaje de estudiantes con Necesidades Educativas Especiales (de ahora en adelante también llamadas NEE), por lo que decidimos investigar acerca de las metodologías de inclusión utilizadas en el 6to año básico B dado que este curso integrado por 18 estudiantes presenta un alto número de estudiantes con NEE. La Escuela Carlos Spano cuenta con un Programa de Integración Escolar (de ahora en adelante también mencionado como PIE) que se rige bajo la Ley de Inclusión Escolar de Chile n°20.845, la que decreta un número de 7 estudiantes por curso acogidos por éste, 2 en modalidad permanente y 5 en transitoria. El objetivo principal de esta investigación es identificar las metodologías de inclusión utilizadas en el 6to año básico B de la Escuela Carlos Spano de Talca, estos mismos forman parte de la comunidad estudiada, además de los docentes del área de matemáticas, lenguaje y música. Los instrumentos de investigación aplicados para obtener los datos a analizar durante este trabajo de grado fueron: entrevistas a profesores ya antes mencionados, entrevistas a funcionarias del PIE, listas de observación de clases y encuestas a los estudiantes pertenecientes al curso observado. En las conclusiones podremos ver el resultado de la implementación de metodologías utilizadas en el 6to año básico B de la Escuela Carlos Spano de Talca, tanto por el PIE como por el establecimiento, viéndose reflejado en el sentir de los/as estudiantes en el ámbito escolar

Latin American aquatic mammals : an overview of 12 years focusing on molecular techniques applied to conservation

Ecological information useful for conservation purposes have benefitted from recent and rapid advancements in genetic techniques, revealing unknown aspects of behavior, natural history, population structure and demography of several aquatic mammal species, many of them with conservation concerns. Molecular markers have been used to define management units, to settle taxonomic uncertainties, to control illegal wildlife trade, among others, providing valuable information to decision-making to conserve and manage aquatic mammals. We review genetic studies applied to conservation-related issues involving natural populations of more than 40 species of aquatic mammals in Latin America, covering four taxonomic groups. The main goal was to assess which genetic approaches have been used and to identify gaps in genetic research relating to geographic areas and species. We reviewed studies published in peer-reviewed journals between 2011 and 2022, and found that most were focused on population structure, phylogeography, gene flow and dispersal movements. The review revealed that researchers need to increase and improve the knowledge in those species which face major conservation concern. Scarce findings were related to forensics and its application to wildlife trade. In the era of next-generation-sequencing techniques, just a few studies used genomics as a tool for monitoring gene diversity, an important goal to help us predict how species will cope with climate change events. Looking to the future we suggest which species, geographic areas and genetic studies should be prioritized in a scenario of climate change and increased human threats (e.g., fishery bycatch, habitat degradation, etc.) and the urgent need for conservation actions. Finally, we highlight the benefits of the collaborative works and the necessity of generating a conservation genetic network, with an open agenda to discuss the local and regional problematics. All in all, we strongly emphasize the generation of critical information towards the effective conservation and management of aquatic mammals in Latin America.Peer reviewe

Enabling high-quality transparent conductive oxide on 3D printed ZrO2 architectures through atomic layer deposition

The conformal atomic layer deposition of a transparent conductive oxide composed of Al-doped ZnO (AZO) over three-dimensional (3D) shaped ZrO2 microarchitectures produced using two-photon lithography (TPL) is reported here for the first time. The effect of ZrO2 morphology, surface roughness, and crystallographic phase (tetragonal and monoclinic) on the quality and properties of the deposited ZnO and AZO thin films is investigated. No discontinuities, domains, or areas differing from the desired chemical composition have been found in films grown over the 3D structures. Three different Al dopant concentrations (4.0 %, 4-5 %, and 5.0 % Al doping cycles) are examined and compared to undoped ZnO. AZO and ZnO optical and electrical properties are studied using cathodoluminescence (CL) and Hall effect measurements. The CL study confirms that the observed emissions from the ZnO and AZO films are associated with the near band emission of ZnO and defects, i.e., zinc and oxygen vacancies and interstitial oxygen. The AZO films exhibit n-type semiconductor behavior, and a minimum resistivity of 1.2 x 10-3 Ω cm is achieved. From a broad perspective, AZO deposition on 3D microarchitectures opens a new route towards dimensionally refined optoelectronic devices in which the ZrO2/AZO can serve a key enabling role for the production of electrodes

Methylation of subtelomeric chromatin modifies the expression of the lncRNA TERRA, disturbing telomere homeostasis

The long noncoding RNA (lncRNA) telomeric repeat-containing RNA (TERRA) has been associated with telomeric homeostasis, telomerase recruitment, and the process of chromosome healing; nevertheless, the impact of this association has not been investigated during the carcinogenic process. Determining whether changes in TERRA expression are a cause or a consequence of cell transformation is a complex task because studies are usually carried out using either cancerous cells or tumor samples. To determine the role of this lncRNA in cellular aging and chromosome healing, we evaluated telomeric integrity and TERRA expression during the establishment of a clone of untransformed myeloid cells. We found that reduced expression of TERRA disturbed the telomeric homeostasis of certain loci, but the expression of the lncRNA was affected only when the methylation of subtelomeric bivalent chromatin domains was compromised. We conclude that the disruption in TERRA homeostasis is a consequence of cellular transformation and that changes in its expression profile can lead to telomeric and genomic instability

The Proteome of Biologically Active Membrane Vesicles from Piscirickettsia salmonis LF-89 Type Strain Identifies Plasmid-Encoded Putative Toxins

Piscirickettsia salmonis is the predominant bacterial pathogen affecting the Chilean salmonid industry. This bacterium is the etiological agent of piscirickettsiosis, a significant fish disease. Membrane vesicles (MVs) released by P. salmonis deliver several virulence factors to host cells. To improve on existing knowledge for the pathogenicity-associated functions of P. salmonis MVs, we studied the proteome of purified MVs from the P. salmonis LF-89 type strain using multidimensional protein identification technology. Initially, the cytotoxicity of different MV concentration purified from P. salmonis LF-89 was confirmed in an in vivo adult zebrafish infection model. The cumulative mortality of zebrafish injected with MVs showed a dose-dependent pattern. Analyses identified 452 proteins of different subcellular origins; most of them were associated with the cytoplasmic compartment and were mainly related to key functions for pathogen survival. Interestingly, previously unidentified putative virulence-related proteins were identified in P. salmonis MVs, such as outer membrane porin F and hemolysin. Additionally, five amino acid sequences corresponding to the Bordetella pertussis toxin subunit 1 and two amino acid sequences corresponding to the heat-labile enterotoxin alpha chain of Escherichia coli were located in the P. salmonis MV proteome. Curiously, these putative toxins were located in a plasmid region of P. salmonis LF-89. Based on the identified proteins, we propose that the protein composition of P. salmonis LF-89 MVs could reflect total protein characteristics of this P. salmonis type strain