On the Presence of Thermal SZ Induced Signal in the First Year WMAP Temperature Maps

Using available optical and X-ray catalogues of clusters and superclusters of galaxies, we build templates of tSZ emission as they should be detected by the WMAP experiment. We compute the cross-correlation of our templates with WMAP temperature maps, and interpret our results separately for clusters and for superclusters of galaxies. For clusters of galaxies, we claim 2-5 $\sigma$ detections in our templates built from BCS Ebeling et al. (1998), NORAS (Boehringer et al. 2000) and de Grandi et al. (1999) catalogues. In these templates, the typical cluster temperature decrements in WMAP maps are around 15-35 $\mu$K in the RJ range (no beam deconvolution applied). Several tests probing the possible influence of foregrounds in our analyses demonstrate that our results are robust against galactic contamination. On supercluster scales, we detect a diffuse component in the V & W WMAP bands which cannot be generated by superclusters in our catalogues (Einasto et al. 1994, 1997), and which is not present in the clean map of Tegmark, de Oliveira-Costa & Hamilton (2003). Using this clean map, our analyses yield, for Einasto's supercluster catalogues, the following upper limit for the comptonization parameter associated to supercluster scales: y_{SC} < 2.18 \time s 10^{-8} at the 95% confidence limit.Comment: MNRAS accepted. New section and minor changes include

Limits on Hot Intracluster Gas Contributions to the Tenerife Temperature Anisotropy Map

We limit the contribution of the hot intracluster gas, by means of the Sunyaev-Zel'dovich effect, to the temperature anisotropies measured by the Tenerife experiment. The data is cross-correlated with maps generated from the ACO cluster catalogue, the ROSAT PSPC catalogue of clusters of galaxies, a catalogue of superclusters and the HEAO 1 A-1 map of X-ray sources. There is no evidence of contamination by such sources at an rms level of $\sim 8\mu$K at 99% confidence level at $5^o$ angular resolution. We place an upper limit on the mean Comptonization parameter of $y \le 1.5\times 10^{-6}$ at the same level of confidence. These limits are slightly more restrictive than those previously found by a similar analysis on the COBE/DMR data and indicate that most of the signal measured by Tenerife is cosmological.Comment: To be published in ApJ (main journal

Fourier Mukai Transforms for Gorenstein Schemes

We extend to singular schemes with Gorenstein singularities or fibered in schemes of that kind Bondal and Orlov's criterion for an integral functor to be fully faithful. We also contemplate a criterion for equivalence. We offer a proof that is new even if we restrict to the smooth case. In addition, we prove that for locally projective Gorenstein morphisms, a relative integral functor is fully faithful if and only if its restriction to each fibre also is it. These results imply the invertibility of the usual relative Fourier-Mukai transform for an elliptic fibration as a direct corollary.Comment: Final version. To appear in Advances in Mathematic

2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador

Background: Rhodnius ecuadoriensis is the main triatomine vector of Chagas disease, American trypanosomiasis, in Southern Ecuador and Northern Peru. Genomic approaches and next generation sequencing technologies have become powerful tools for investigating population diversity and structure which is a key consideration for vector control. Here we assess the effectiveness of three different 2b restriction site-associated DNA (2b-RAD) genotyping strategies in R. ecuadoriensis to provide sufficient genomic resolution to tease apart microevolutionary processes and undertake some pilot population genomic analyses. Methodology/Principal findings: The 2b-RAD protocol was carried out in-house at a non-specialized laboratory using 20 R. ecuadoriensis adults collected from the central coast and southern Andean region of Ecuador, from June 2006 to July 2013. 2b-RAD sequencing data was performed on an Illumina MiSeq instrument and analyzed with the STACKS de novo pipeline for loci assembly and Single Nucleotide Polymorphism (SNP) discovery. Preliminary population genomic analyses (global AMOVA and Bayesian clustering) were implemented. Our results showed that the 2b-RAD genotyping protocol is effective for R. ecuadoriensis and likely for other triatomine species. However, only BcgI and CspCI restriction enzymes provided a number of markers suitable for population genomic analysis at the read depth we generated. Our preliminary genomic analyses detected a signal of genetic structuring across the study area. Conclusions/Significance: Our findings suggest that 2b-RAD genotyping is both a cost effective and methodologically simple approach for generating high resolution genomic data for Chagas disease vectors with the power to distinguish between different vector populations at epidemiologically relevant scales. As such, 2b-RAD represents a powerful tool in the hands of medical entomologists with limited access to specialized molecular biological equipment. Author summary: Understanding Chagas disease vector (triatomine) population dispersal is key for the design of control measures tailored for the epidemiological situation of a particular region. In Ecuador, Rhodnius ecuadoriensis is a cause of concern for Chagas disease transmission, since it is widely distributed from the central coast to southern Ecuador. Here, a genome-wide sequencing (2b-RAD) approach was performed in 20 specimens from four communities from Manabí (central coast) and Loja (southern) provinces of Ecuador, and the effectiveness of three type IIB restriction enzymes was assessed. The findings of this study show that this genotyping methodology is cost effective in R. ecuadoriensis and likely in other triatomine species. In addition, preliminary population genomic analysis results detected a signal of population structure among geographically distinct communities and genetic variability within communities. As such, 2b-RAD shows significant promise as a relatively low-tech solution for determination of vector population genomics, dynamics, and spread

L'arbre fontaine

Dans certaines régions montagneuses régulièrement ventées, soumises à un climat océanique comme au Chili, en Afrique du Sud ou au Cap-Vert, une mer de brouillard règne en quasi permanence. Ces conditions météorologiques très singulières permettent à la végétation de prospérer malgré de faibles pluies. Arbres et arbustes captent l'eau des brouillards et des bruines, donnant naissance à de véritables forêts dites nébuleuses. Ces fontaines végétales sont exploitées par l'homme depuis la nuit des temps pour récupérer de l'eau potable et développer une vie agricole. Véritable don des dieux, l'arbre fontaine a parfois été élevé au rang d'arbre sacré comme dans l'île de Hierro aux Canaries, point de départ de la recherche des auteurs de cet article. (Résumé d'auteur

L-arginine: A unique amino acid for improving depressed wound immune function following hemorrhage

Objective: To determine whether L-arginine has any salutary effects on wound immune cell function following trauma-hemorrhage. Background. Depressed wound immune function contributes to an increased incidence of wound infections following hemorrhage. Although administration of L-arginine has been shown to restore depressed cell-mediated immune responses following hemorrhage potentially by maintaining organ blood flow, it remains unknown whether Larginine has any salutary effects on the depressed local immune response at the wound site. Methods: Male mice were subjected to a midline laparotomy and polyvinyl sponges were implanted subcutaneously in the abdominal wound prior to hemorrhage (35 +/- 5 mm Hg for 90 min and resuscitation) or sham operation. During resuscitation mice received 300 mg/kg body weight L-arginine or saline (vehicle). Sponges were harvested 24 h thereafter, wound fluid collected and wound immune cells cultured for 24 h in the presence of LPS. Pro- (IL-1beta, IL-6) and anti-inflammatory (IL-10) cytokines were determined in the supernatants and the wound fluid. In addition, wounds were stained for IL-6 immunohistochemically. In a separate set of animals, skin and muscle blood flow was determined by microspheres. Results: The capacity of wound immune cells to release IL-1beta and IL-6 in vitro was significantly depressed in hemorrhaged mice receiving vehicle. Administration of L-arginine, however, improved wound immune cell function. In contrast, in vivo the increased IL-6 release at the wound site was decreased in L-arginine-treated mice following hemorrhage. Moreover, IL-10 levels were significantly increased in the wound fluid in hemorrhaged animals receiving L-arginine compared to vehicle-treated mice. In addition, the depressed skin and muscle blood flow after hemorrhage was restored by L-arginine. Conclusions: Thus, L-arginine might improve local wound cell function by decreasing the inflammatory response at the wound site. Since L-arginine protected wound immune cell function this amino acid might represent a novel and useful adjunct to fluid resuscitation for decreasing wound complications following hemorrhage. Copyright beta 2002 S. Karger AG, Basel