Development of a temporary marker for peptides

3-[(N,N-dimethylaminophenyl)-4'-diazenyl]-benzoic acid was coupled with several amino acid esters and the product further acylated with Boc. The material thus obtained was then submitted to cleavage by electrolysis and nucleophilic attack in order to evaluate the possibility to use this chromophore as a temporary marker

A marcação CE e as directivas nova abordagem

Mestrado em GestãoO aumento das importações de países como a China tem provocado um escalar de alguns dissabores e problemas de qualidade a que os empresários não estavam habituados. A capacidade de produzir a um baixo custo, com prazos de entrega e quantidades muito significativas tem-se mostrado de uma atractividade difícil de resistir. No entanto, problemas graves de falta de qualidade têm sido denunciados um pouco por todo o mundo e tem-se tornado fundamental o fortalecimento da legislação de forma a proteger os interesses e a saúde dos consumidores. É aqui que entra a Marcação CE, as Directivas Nova Abordagem e as Normas Harmonizadas, como resposta legislativa da União Europeia à necessidade, não apenas, de ultrapassar barreiras técnicas imprescindíveis à livre circulação, mas também como forma eficaz de harmonização legislativa, capaz de indicar a conformidade de um produto com os requisitos essenciais mínimos fundamentais para assegurar a protecção do interesse público. A Marcação CE significa que um produto foi fabricado de acordo com as normas europeias e que os requisitos essenciais definidos na norma aplicável foram cumpridos, tornando-o assim num produto seguro de utilizar. Desta forma, a sua aposição ou exigência deverá ser suficiente para assegurar que um produto está apto para o fim a que se destina. Este trabalho tem como objectivos principais, em primeiro lugar compreender as dinâmicas do processo de Marcação CE e os seus objectivos de uma forma teórica, analisando a legislação que a regula e compilando informação importante e dispersa, e em segundo lugar descrever através de um estudo caso, a melhor forma de obter informação acerca das Directivas e normas que se aplicam a um produto.The increase on imports of countries as China has been provoking the skyrocketing of drawbacks and quality problems that most managers were not used to. The capacity to produce at low costs, with short delivery times and with unlimited production capacity has revealed itself very difficult to cope with by competitors. However, serious problems with product quality have been denounced everywhere, which has motivated the invigoration of the legislation in order to protect the interests and the consumers’ health. It is here that the CE marking, New Directives Approach as well as the Harmonized standards come to play as legislative answer from the European Union to the need not only for overtaking indispensable technical barriers to the free circulation, but also as effective form of legislative harmonization, capable to indicate the conformance of a product with the fundamental minimum essential requirements to assure the public interest protection. The CE Marking means that a product was manufactured in agreement with the European standards and that the essential requirements defined in the applicable standards were implemented, making the product a safe one. This way, its apposition or demand should be enough to assure that a product is capable for the purpose it was defined to. This work has as main objective: firstly, to understand the dynamics of the CE marking process and its objectives, and secondly, to describe through a study case, the best way of obtaining information concerning Directives and standards that are applied to a product

Linear and non-linear analysis of uterine contraction signals obtained with tocodynamometry in prediction of operative vaginal delivery

The aim of this study was to explore whether linear and non-linear analysis of uterine contraction (UC) signals obtained with external tocodynamometry can predict operative vaginal delivery (OVD).Materials and methods: The last 2 h before delivery (H1 and H2) of 55 UC recordings acquired with external tocodynamometry in the labour ward of a tertiary care hospital were analysed. Signal processing involved the quantification of UCs/segment (UCN), and the linear and non-linear indices: Sample Entropy (SampEn) measuring signal irregularity; interval index (II) measuring signal variability, both of which may be associated with uterine muscle fatigue, and high frequency (HF), associated with maternal breathing movements. Thirty-two women had normal deliveries and 23 OVDs. Statistical inference was performed using 95% confidence intervals (95% CIs) for the median, and areas under the receiver operating curves (auROCs), with univariate and bivariate analyses. Results: A significant association was found between maternal body mass index (BMI) and UC signal quality in H1, with moderate/poor signal quality being more frequente with higher maternal BMI. There was an overall increase in contraction frequency (UCN), signal regularity (SampEn), signal variability (II), and maternal breathing (HF) from H1 to H2. The OVD group exhibited significantly higher values of signal irregularity and variability (SampEn and II) in H1, and higher contraction frequency (UCN) and maternal breathing (HF) in H2. Modest auROCs ere obtained with these indices in the discrimination between normal and OVDs. Conclusions: The results of this exploratory study suggest that analysis of UC signals obtained with tocodynamometry, using linear and non-linear indices associated with muscle fatigue and maternal breathing, identifies significant changes occurring during labour, and diferences between normal and OVDs, but their discriminative capacity between the two types of delivery is modest. Further refinement of this analysis is needed before it may be clinically useful.info:eu-repo/semantics/publishedVersio

Electrocardiography versus photoplethysmography in assessment of maternal heart rate variability during labor

Evaluation of maternal heart rate (MHR) variability provides useful information on the maternal-fetal clinical state. Electrocardiography (ECG) is the most accurate method to monitor MHR but it may not always be available, and pulse oximetry using photoplethysmography (PPG) can be an alternative. In this study we compared ECG and PPG signals, obtained with conventional fetal monitors, to evaluate signal loss, MHR variability indices, and the ability of the latter to predict fetal acidemia and operative delivery.info:eu-repo/semantics/publishedVersio

Sex differences in the fetal heart rate variability indices of twins

Aims: To evaluate the differences in linear and complex heart rate dynamics in twin pairs according to fetal sex combination [male-female (MF), male-male (MM), and female-female (FF)]. Methods: Fourteen twin pairs (6 MF, 3 MM, and 5 FF) were monitored between 31 and 36.4 weeks of gestation. Twenty-six fetal heart rate (FHR) recordings of both twins were simultaneously acquired and analyzed with a system for computerized analysis of cardiotocograms. Linear and nonlinear FHR indices were calculated. Results: Overall, MM twins presented higher intrapair average in linear indices than the other pairs, whereas FF twins showed higher sympathetic-vagal balance. MF twins exhibited higher intrapair average in entropy indices and MM twins presented lower entropy values than FF twins considering the (automatically selected) threshold rLu. MM twin pairs showed higher intrapair differences in linear heart rate indices than MF and FF twins, whereas FF twins exhibited lower intrapair differences in entropy indices. Conclusions: The results of this exploratory study suggest that twins have sex-specific differences in linear and nonlinear indices of FHR. MM twins expressed signs of a more active autonomic nervous system and MF twins showed the most active complexity control system. These results suggest that fetal sex combination should be taken into consideration when performing detailed evaluation of the FHR in twins.This work was supported by a grant (SFRH/BD/40146/2007) to the first author from Fundacao para a Ciencia e Tecnologia. Hernani Goncalves is financed by a postdoctoral grant (SFRH/BPD/69671/2010) from the Fundacao para a Ciencia e a Tecnologia (FCT), Portugal

Atas das 8as Jornadas de Segurança aos Incêndios Urbanos e as 3as Jornadas de Proteção Civil (8JORNINC-3JORPROCIV)

Este livro de ATAS contém os artigos apresentados às 8as Jornadas de Segurança aos Incêndios Urbanos e às 3as Jornadas de Proteção Civil (8JORNINC-3JORPROCIV), que decorreram no Porto, Portugal. Na presente edição das 8JORNINC-3JORPROCIV foram submetidos 50 trabalhos, tendo sido aceites 42. Os trabalhos foram distribuídos em 8 sessões paralelas temáticas, em adição a 2 sessões plenárias, apresentados no dia 2 de junho de 2023. O evento foi iniciado e promovido em Portugal, sob a organização do professor Doutor João Paulo Rodrigues, com as 1as Jornadas de Segurança aos Incêndios Urbanos, em 2005, na Universidade de Coimbra, bem como nos anos seguintes, as 2as Jornadas de Segurança aos Incêndios Urbanos em 2011 e as 3as Jornadas de Segurança aos Incêndios Urbanos em 2013, também na Universidade de Coimbra. As edições seguintes aconteceram em diferentes locais: as 4as Jornadas de Segurança aos Incêndios Urbanos em 2014, no Instituto Politécnico de Bragança; as 5as Jornadas de Segurança aos Incêndios Urbanos em 2016, no Laboratório Nacional de Engenharia Civil em Lisboa; as 6as Jornadas de Segurança aos Incêndios Urbanos e 1as Jornadas de Proteção Civil em 2018, na Universidade de Coimbra; e as 7as Jornadas de Segurança aos Incêndios Urbanos e 2as Jornadas de Proteção Civil, em 2021, no Instituto Politécnico de Castelo Branco. As 8JORNINC apresentam grande importância num contexto atual de vários e graves incêndios urbanos, florestais e de interface em Portugal. A pertinência do tema da segurança na prevenção e no combate a incêndios, quer pelas consequências emergentes deste tipo de acidentes, quer pela necessidade de redução das ocorrências, do número de vítimas mortais, feridos, prejuízos materiais, patrimoniais, ambientais e sociais, leva a que as Jornadas se destinem a um leque alargado de profissionais e público em geral. As 3JORPROCIV têm como objetivo promover conhecimentos nas áreas da prevenção civil, de riscos e planos de emergência. Pretendem assim, contribuir para a atualização dos conhecimentos técnicos e científicos da segurança e proteção civil, no âmbito do planeamento e prevenção perante cenários de crise e emergência. As Jornadas de Proteção Civil permitem partilhar um leque de conhecimentos multidisciplinares suscetíveis de impulsionar uma intervenção por parte de técnicos, especialistas e dos agentes da proteção civil. A visão interdisciplinar e integradora dos problemas e desafios que a proteção civil apresenta será refletida na prevenção e mitigação dos riscos inerentes a uma situação de acidente. Os trabalhos apresentados nesta edição, permitirão o avanço das 8JORNINC-3JORPROCIV, através da divulgação dos recentes desenvolvimentos e do conhecimento nos domínios da segurança ao incêndio e proteção civil. Por fim, a comissão organizadora das 8JORNINC-3JORPROCIV gostaria de agradecer: - o apoio dos patrocinadores e das instituições do Sistema Científico e Tecnológico; - a todos os autores que partilharam os seus excelentes trabalhos; - e aos elementos da Comissão Científica que auxiliaram no processo de revisão. Todos em conjunto, tornaram possível a realização destas Jornadas.info:eu-repo/semantics/publishedVersio

Subsyndromal delirium in critically ill patients: cognitive and functional long-term outcomes

© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Subsyndromal delirium (SSD) in the Intensive Care Unit (ICU) is associated with an increased morbidity with unknown post-discharge functional and cognitive outcomes. We performed a prospective multicenter study to analyze the mental status of patients during their first 72 h after ICU admission and its trajectory, with follow-ups at 3 and 6 months after hospital discharge. Amongst the 106 included patients, SSD occurred in 24.5% (n = 26) and was associated with the duration of mechanical ventilation (p = 0.003) and the length of the ICU stay (p = 0.002). After the initial 72 h, most of the SSD patients (30.8%) improved and no longer had SSD; 19.2% continued to experience SSD and one patient (3.8%) progressed to delirium. The post-hospital discharge survival rate for the SSD patients was 100% at 3 months and 87.5% at 6 months. At admission, 96.2% of the SSD patients were fully independent in daily living activities, 66.7% at 3-month follow-up, and 100% at 6-month follow-up. Most SSD patients demonstrated a cognitive decline from admission to 3-month follow-up and improved at 6 months (IQCODE-SF: admission 3.13, p < 0.001; 3 months 3.41, p = 0.019; 6 months 3.19, p = 0.194). We concluded that early SSD is associated with worse outcomes, mainly a transitory cognitive decline after hospital discharge at 3 months, with an improvement at 6 months. This highlights the need to prevent and identify this condition during ICU stays.info:eu-repo/semantics/publishedVersio

Reaction of carboxylic dyes with wool and polyamide. Part III: Effect of the activating agent

Dyes containing a carboxylic acid group had been shown to react with wool and polyamide fibres when activated with ethyl chloroformate (Parts I and II). One of the dyes, 3-aminobenzoic acid →N,N-dimethylaniline, was, in this work, activated with other chlorofirmates, so as to improve the dyeing conditions. Benzyl chloroformate was found to be a good substitute since it is not as volatile as ethyl chloroformate, which suggests that it will be easier to apply in practical dyeing conditions. The yield of the reaction with cyclohexylamine is similar to the one obtained with ethyl chloroformate, suggesting that the fixation of the dye on wool or polyamide will be much the same. The fastness results are also equivalent.FCT-Fundação para a Ciência e Tecnologi

Caracterização da variabilidade da frequência cardíaca através de métodos lineares e não-lineares

Tese de mestrado. Métodos Computacionais em Ciências e Engenharia. 2004. Faculdade de Engenharia. Universidade do Port