Release of antioxidant peptides from the body wall proteins of the sea cucumber Isostichopus fuscus

Proteases from the tentacles of the sea cucumber Isostichopus fuscus were partially purified and used to produce antioxidant peptides from body wall proteins of this marine species. Three proteins (105, 68, and 39 kDa) were identified by SDS-PAGE in the proteolytic extract of the tentacles. Protein hydrolyzates were generated with gelatin and crude protein substrates from body wall, and peptidic fractions lower and higher than 3 kDa were obtained to evaluate their oxygen radical scavenging capacity (ORAC). The 3 kDa-fraction obtained from the crude protein hydrolyzate showed the highest ORAC value (0.92 ± 0.04 μmol Trolox equivalent/mg protein). This fraction was selected to purify peptides potentially responsible for the activity that might be used as ingredients for development of functional foods.A.C.H.-S thanks the National Council of Science and Technology (CONACYT, Mexico) for a graduate scholarship and the “Universidad Autónoma Metropolitana” for giving the marine organisms. B.H.-L. thanks the Ministry of Economy and Competitiveness (MINECO) and the Spanish National Research Council (CSIC) for her “Ramón y Cajal” postdoctoral contract.Peer Reviewe

Partial characterization of a proteolytic extract obtained from sea cucumber, and generation of ovalbumin hydrolysates

Resumen del póster presentado al II Congreso Internacional de Investigación e Innovación en Ingeniería, Ciencia y Tecnología de Alimentos celebrado en Medellín (Colombia) del 27 al 30 de mayo de 2014.[ES]: El objetivo de este trabajo fue caracterizar parcialmente el extracto proteolítico del intestino de pepino de mar Isostichopus fuscus y generar hidrolizados a partir de ovoalbúmina. Los estudios de zimografía confirmaron la presencia de proteasas en el extracto de pepino de mar, identificándose proteínas de 109, 42, y 39 kDa. La actividad proteolítica máxima se observó a pH 6 y 8; a 40 y 70°C. Se obtuvieron hidrolizados de ovoalbúmina a 60 y 7°C y se analizaron por RP-HPLC y SDS-PAGE. El uso de proteasas marinas con alta actividad a baja temperatura puede presentar posibles ventajas para la industria de alimentos. [EN]: The objective of this work was partially characterizing a proteolytic extract obtained from sea cucumber Isostichopus fuscus digestive system, and generating ovalbumin hydrolysates. Zymograms confirm the presence of proteases (109, 42, y 39 kDa). The highest activity was observed at pH 6 and 8; at 40 and 70 °C. Ovalbumin hydrolysates were obtained at 60 and 7 °C, and analyzed by RP-HPLC and SDSPAGE. Application of marine proteases with high activity at low temperatures could be an advantage for food application.Peer Reviewe

Pituitary Abscess Causing Panhypopituitarism in a Patient With Neurobrucellosis: Case Report

Background/Objective: Pituitary abscess is an uncommon life-threatening disease that could lead to panhypopituitarism. It is important to suspect its prevalence in regions with endemic infectious diseases. Case Report: A 55-year-old man, a farmer, with a background of consumption of unpasteurized dairy products, presented with headache, impaired consciousness, and fever that started in February 2023. Initial test results were consistent with neuroinfection. Brain MRI showed ventriculitis; the pituitary gland was heterogeneous with the presence of an 8 × 8 mm abscess. The pituitary hormone axis was evaluated, and it showed results compatible with the results of panhypopituitarism with central hypothyroidism, central hypocortisolism, central hypogonadism, and growth hormone deficiency. Hormone replacement treatment with hydrocortisone and levothyroxine was started. The Rose Bengal test for Brucella spp. and 2-mercaptoethanol Brucella agglutination test showed positive results. After neurobrucellosis (NB) was diagnosed, antibiotic treatment was commenced. The patient was discharged 6 weeks later and treatment with prednisone, levothyroxine, recombinant somatropin, testosterone, as well as doxycycline, and rifampin was continued for another 4 months. Discussion: NB and pituitary abscess are rare manifestations of brucellosis and are challenging to diagnose due to their nonspecific clinical presentation and cerebrospinal fluid (CSF) findings. NB diagnosis relies on neurologic symptoms and serological evidence of Brucella infection. Magnetic resonance imaging is the preferred diagnostic tool for pituitary abscesses. Medical management may be sufficient, while transsphenoidal drainage is not always necessary. Hormonal deficits typically remain permanent. Conclusion: Pituitary abscess could be suspected in patients presenting with symptoms of neuroinfection, panhypopituitarism, and heterogenous image in the magnetic resonance imaging differential diagnosis. Opportune management can lead to reduced mortality and improved recovery of the pituitary hormone function

Tolerance Induction to Antituberculosis Drugs in a Patient With Stevens–Johnson Syndrome/Toxic Epidermal Necrolysis Overlap

Tolerance induction and desensitization in Stevens–Johnson syndrome (SJS) or in toxic epidermal necrolysis (TEN) have been described as an absolute contraindication by some authors, but there are cases where there is no treatment alternative. Tuberculosis (TB) remains a leading cause of morbidity and mortality in developing countries and ranks alongside HIV as a leading cause of death worldwide. Severe drug reactions, such as SJS and TEN, occurring in these individuals are lifethreatening. Since alternative therapies for TB are limited, the role of desensitization and reintroduction becomes essential. We describe a case of tolerance induction to anti-TB drugs in a patient with SJS/TEN overlap syndrome using a specifically designed premedication, comedication, and desensitization protocol

Progama para la conservación y manejo sustentable de la subcuenca Tejalpa en la porción del Nevado de Toluca

29 p