Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells

The main vector of dengue in America is the mosquito Aedes aegypti, which is infected by dengue virus (DENV) through receptors of midgut epithelial cells. The envelope protein (E) of dengue virus binds to receptors present on the host cells through its domain III that has been primarily recognized to bind cell receptors. In order to identify potential receptors, proteins from mosquito midgut tissue and C6/36 cells were purified by affinity using columns with the recombinant E protein domain III (rE-DIII) or DENV particles bound covalently to Sepharose 4B to compare and evaluate their performance to bind proteins including putative receptors from female mosquitoes of Ae. aegypti. To determine their identity mass spectrometric analysis of purified proteins separated by polyacrylamide gel electrophoresis was performed. Our results indicate that both viral particles and rE-DIII bound proteins with the same apparent molecular weights of 57 and 67 kDa. In addition, viral particles bound high molecular weight proteins. Purified proteins identified were enolase, beta-adrenergic receptor kinase (beta-ARK), translation elongation factor EF-1 alpha/Tu, and cadherin

Evaluación histopatológica en modelo murino suplementado con creatina

Creatine is a natural nutrient, made up of three amino acids (glycine, arginine and methionine), it can be synthesized endogenously in the pancreas, liver and kidney, or it can also be obtained from the diet with the consumption of foods of animal origin (meat and fish), 95 percent of the creatine in the body can be located in skeletal muscle. The objective of the work was to determine histologically the effect of creatine (α-methyl guanido-acetic acid or creatine monohydrate) in a mouse model. As a methodology, an experimental, longitudinal, analytical and descriptive study was carried out, 3 groups of animals with 5 each were used, in which 3 different doses of creatine were tested for 60 days, at the end of the time they were sacrificed and anthropometric measurements were obtained. and histological sections. The results showed that there is obesity in the study groups, in terms of histology, the microscopic images observed in the control groups did not show global abnormalities in their structural morphology, indicating that the manipulation and/or exposure times did not modify the architecture of the muscle, large intestine, pancreas, liver, kidney, skin, lung, and small intestine. It can be concluded that at the doses used, creatine is safe.La creatina es un nutriente natural, formado por tres aminoácidos (glicina, arginina y metionina), se puede sintetizar endógenamente en páncreas, hígado y riñón, o también se pude obtener dentro de la dieta con el consumo de alimentos de origen animal (carnes y pescado), el 95 por ciento de la creatina en el organismo se puede localizar en el musculo esquelético. El objetivo del trabajo fue determinar histológicamente el efecto de la creatina (ácido α-metil guanido-acético o monohidrato de creatina) en modelo murino. Como metodología se realizó un estudio experimental, longitudinal, analítico y descriptivo, se utilizaron 3 grupos de animales con 5 cada uno, en los cuales durante 60 días se probaron 3 diferentes dosis de creatina, al término del tiempo se sacrificaron y se obtuvieron medidas antropométricas y cortes histológicos. Los resultados arrojaron que existe obesidad en los grupos de estudio, en cuanto a la histología las imágenes microscópicas observadas de los grupos control no mostraron anormalidades globales en su morfología estructural, indicando que la manipulación y/o los tiempos de exposición no modificaron la arquitectura del musculo, intestino grueso, páncreas, hígado, riñón, piel, pulmón e intestino delgado. Se puede concluir que a las dosis utilizadas la creatina es segura

The rs12617336 and rs17574 Dipeptidyl Peptidase-4 Polymorphisms Are Associated With Hypoalphalipoproteinemia and Dipeptidyl Peptidase-4 Serum Levels: A Case-Control Study of the Genetics of Atherosclerotic Disease (GEA) Cohort

Dipeptidyl peptidase-4 (DPP4) can influence lipid homeostasis and atherosclerosis progression. We aimed to assess the association of DPP4 gene polymorphisms with hypoalphalipoproteinemia and DPP4 serum levels, in a cohort of Mexican individuals. Five DPP4 polymorphisms (rs12617336, rs12617656, rs1558957, and rs3788979, and rs17574) were genotyped in 748 participants with and 745 without hypoalphalipoproteinemia. The associations were evaluated using logistic regression analyses. Under inheritance models adjusted for confounding variables, the rs12617336 (OR = 0.22, Pheterozygote = 0.001) and rs17574 (OR = 0.78, Padditive = 0.022; OR = 0.73, Pdominant = 0.012; OR = 0.73, Pheterozygote = 0.017; OR = 0.72, Pcodominant1 = 0.014) minor alleles were associated with a low risk of hypoalphalipoproteinemia. After the correction for multiple comparisons, the associations were marginal except the association of the rs12617336 that remaining significant. Additionally, both DPP4 minor alleles were associated with protection for the presence of insulin resistance (IR) (OR = 0.17, Pheterozygote = 0.019 for rs12617336 and OR = 0.75, Padditive = 0.049 for rs17574). The rs12617336 minor allele was also associated with a low risk of hyperinsulinemia (OR = 0.11, Pheterozygote = 0.006). Differences in DPP4 levels were observed in individuals with rs17574 genotypes, the rs17574 GG genotype individuals had the lowest levels. Our data suggest that rs12617336 and rs17574 DPP4 minor alleles could be envisaged as protective genetic markers for hypoalphalipoproteinemia, IR, and hyperinsulinemia. The rs17574 GG genotype was associated with the lowest DPP4 levels

Ciencia, Tecnología y Salud en la Atención de los Adolescentes

El Centro de Investigación en Ciencias Médicas (CICMED) de la Universidad Autónoma del Estado de México, institución que se distingue por su preocupación en el desarrollo de proyectos de investigación, actividades académicas y de vinculación encaminadas a la detección y tratamiento de aquellos problemas que aquejan a los adolescentes, se ha distinguido por la promoción de la salud del adolescente, además de un trabajo intenso a través de redes de investigación con otras instituciones con lo cual se han enriquecido nuestros proyectos de investigación, permitiendo establecer convenios con instituciones tanto nacionales como internacionales. Los temas que se tratan en este libro, han dado lugar a una gran cantidad de reflexiones, mitos, estrategias y propuestas para la cuidado del adolescente, motivo por el cual se invitó tanto a docentes como a investigadores a participar en este libro y poder mostrar lo que se está haciendo actualmente en relación con la atención de la salud del adolescente desde diferentes perspectivas temáticas, con el propósito de difundir ampliamente los hallazgos que como investigadores se han encontrado a través del trabajo clínico y de campo, aportar información relevante para la prevención y tratamiento de la problemática más frecuente en el adolescente y abrir un espacio de intercambio y actualización, basado en el trabajo interdisciplinario para entender mejor y ampliar la visión de los diferentes factores que inciden en la salud integral del adolescente. El adolescente concebido como la persona que está en la transición de la niñez a la vida adulta, ha existido siempre. Sin embargo, la adolescencia entendida como un proceso bio-psico-social con entidad propia y, en consecuencia, con características necesidades y problemas específicos, es una realidad que apenas ha comenzado a tenerse en cuenta recientemente. La adolescencia es el período de tiempo en el que se producen los cambios desde la inmadurez propia del niño a la madurez propia del adulto. Entendiendo al individuo maduro como: aquel que es capaz de orientar su propia vida según el sentido de la existencia, con criterios propios. Desde el punto de vista intelectual o mental, el individuo maduro es aquel que es capaz de juzgar con independencia y objetividad, con sentido crítico. Desde la vertiente afectiva, posee autocontrol de las emociones y es capaz de aceptar los fracasos sin grandes conmociones interiores. Desde el aspecto social, está dispuesto a colaborar en las tareas colectivas, es tolerante con los demás y es capaz de asumir su propia responsabilidad. Esta etapa de la vida de los seres humanos es importante ya que depende del desarrollo armónico de ésta, obliga al futuro adulto en su mundo globalizado lleno de exigencias y riesgos, que los profesionales de la salud reconsideren su actuar con estas personas, dado que hoy en día no hay especialistas o posgraduados que atiendan de manera específica a los adolescentes. En este sentido la Universidad Autónoma del Estado de México, a través del Centro de Investigación en Ciencias Médica adquiere la responsabilidad y el compromiso de motivar a padres, educadores, profesionales de la salud y a los mismos adolescentes para que se conviertan en promotores de la salud física y mental que bajo la misma meta en la escuela y el hogar promuevan una conducta saludable. Por lo que brindamos nuestro agradecimiento a los autores por su valiosa participación y confianza al dejar en nuestras manos su trabajo profesional, comprometido y entusiasta, con el propósito de aportar a la sociedad una perspectiva general sobre el adolescente y sus trastornos para ubicarnos después en un contexto de salud integral

Frequency and management of maternal infection in health facilities in 52 countries (GLOSS): a 1-week inception cohort study

Background: Maternal infections are an important cause of maternal mortality and severe maternal morbidity. We report the main findings of the WHO Global Maternal Sepsis Study, which aimed to assess the frequency of maternal infections in health facilities, according to maternal characteristics and outcomes, and coverage of core practices for early identification and management. Methods: We did a facility-based, prospective, 1-week inception cohort study in 713 health facilities providing obstetric, midwifery, or abortion care, or where women could be admitted because of complications of pregnancy, childbirth, post-partum, or post-abortion, in 52 low-income and middle-income countries (LMICs) and high-income countries (HICs). We obtained data from hospital records for all pregnant or recently pregnant women hospitalised with suspected or confirmed infection. We calculated ratios of infection and infection-related severe maternal outcomes (ie, death or near-miss) per 1000 livebirths and the proportion of intrahospital fatalities across country income groups, as well as the distribution of demographic, obstetric, clinical characteristics and outcomes, and coverage of a set of core practices for identification and management across infection severity groups. Findings: Between Nov 28, 2017, and Dec 4, 2017, of 2965 women assessed for eligibility, 2850 pregnant or recently pregnant women with suspected or confirmed infection were included. 70·4 (95% CI 67·7–73·1) hospitalised women per 1000 livebirths had a maternal infection, and 10·9 (9·8–12·0) women per 1000 livebirths presented with infection-related (underlying or contributing cause) severe maternal outcomes. Highest ratios were observed in LMICs and the lowest in HICs. The proportion of intrahospital fatalities was 6·8% among women with severe maternal outcomes, with the highest proportion in low-income countries. Infection-related maternal deaths represented more than half of the intrahospital deaths. Around two-thirds (63·9%, n=1821) of the women had a complete set of vital signs recorded, or received antimicrobials the day of suspicion or diagnosis of the infection (70·2%, n=1875), without marked differences across severity groups. Interpretation: The frequency of maternal infections requiring management in health facilities is high. Our results suggest that contribution of direct (obstetric) and indirect (non-obstetric) infections to overall maternal deaths is greater than previously thought. Improvement of early identification is urgently needed, as well as prompt management of women with infections in health facilities by implementing effective evidence-based practices.Fil: Althabe, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Epidemiología y Salud Pública. Instituto de Efectividad Clínica y Sanitaria. Centro de Investigaciones en Epidemiología y Salud Pública; ArgentinaFil: Espinoza, Marisa Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Epidemiología y Salud Pública. Instituto de Efectividad Clínica y Sanitaria. Centro de Investigaciones en Epidemiología y Salud Pública; ArgentinaFil: Pasquale, Julia. No especifíca;Fil: Hernández Muñoz, Rosalinda. No especifíca;Fil: Carvajal, Javier. No especifíca;Fil: Escobar, María Fernanda. No especifíca;Fil: Cecatti, José Guilherme. No especifíca;Fil: Ribeiro Do Valle, Carolina C.. No especifíca;Fil: Mereci, Wilson. No especifíca;Fil: Vélez, Paola. No especifíca;Fil: Pérez, Aquilino M.. No especifíca;Fil: Vitureira, Gerardo. No especifíca;Fil: Leroy, Charlotte. No especifíca;Fil: Roelens, Kristien. No especifíca;Fil: Vandenberghe, Griet. No especifíca;Fil: Aguemon, Christiane Tshabu. No especifíca;Fil: Cisse, Kadari. No especifíca;Fil: Ouedraogo, Henri Gautier. No especifíca;Fil: Kannitha, Cheang. No especifíca;Fil: Rathavy, Tung. No especifíca;Fil: Tebeu, Pierre Marie. No especifíca;Fil: Bustillo, Carolina. No especifíca;Fil: Bredy, Lara. No especifíca;Fil: Herrera Maldonado, Nazarea. No especifíca;Fil: Abdosh, Abdulfetah Abdulkadir. No especifíca;Fil: Teklu, Alula M.. No especifíca;Fil: Kassa, Dawit Worku. No especifíca;Fil: Kumar, Vijay. No especifíca;Fil: Suri, Vanita. No especifíca;Fil: Trikha, Sonia. No especifíca

III Congreso de innovación docente en clásicas en la Comunidad de Madrid: Los retos de la enseñanza online

Memoria final del PIMCD 58/202

Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells

The main vector of dengue in America is the mosquito Aedes aegypti, which is infected by dengue virus (DENV) through receptors of midgut epithelial cells. The envelope protein (E) of dengue virus binds to receptors present on the host cells through its domain III that has been primarily recognized to bind cell receptors. In order to identify potential receptors, proteins from mosquito midgut tissue and C6/36 cells were purified by affinity using columns with the recombinant E protein domain III (rE-DIII) or DENV particles bound covalently to Sepharose 4B to compare and evaluate their performance to bind proteins including putative receptors from female mosquitoes of Ae. aegypti. To determine their identity mass spectrometric analysis of purified proteins separated by polyacrylamide gel electrophoresis was performed. Our results indicate that both viral particles and rE-DIII bound proteins with the same apparent molecular weights of 57 and 67 kDa. In addition, viral particles bound high molecular weight proteins. Purified proteins identified were enolase, beta-adrenergic receptor kinase (beta-ARK), translation elongation factor EF-1 alpha/Tu, and cadherin

Association of the rs17574 DPP4 Polymorphism with Premature Coronary Artery Disease in Diabetic Patients: Results from the Cohort of the GEA Mexican Study

Previously, it has been reported that hypoalphalipoproteinemia (HA) is associated with rs17574 DDP4 polymorphism. Considering that in diabetic patients, HA is often present and is a risk factor for premature coronary artery disease (pCAD), the study aimed to evaluate the association of this polymorphism with pCAD in diabetic individuals. We genotyped the rs17574 polymorphism in 405 pCAD patients with T2DM, 736 without T2DM, and 852 normoglycemic individuals without pCAD and T2DM as controls. Serum DPP4 concentration was available in 818 controls, 669 pCAD without T2DM, and 339 pCAD with T2DM. The rs17574 polymorphism was associated with lower risk of pCAD (padditive = 0.007; pdominant = 0.003, pheterozygote = 0.003, pcodominant1 = 0.003). In pCAD with T2DM patients, DPP4 levels were lower when compared with controls (p < 0.001). In the whole sample, individuals with the rs17574 GG genotype have the lowest protein levels compared with AG and AA (p = 0.039) carriers. However, when the same analysis was repeated separately in all groups, a significant difference was observed in the pCAD with T2DM patients; carriers of the GG genotype had the lowest protein levels compared with AG and AA (p = 0.037) genotypes. Our results suggest that in diabetic patients, the rs17574G DPP4 allele could be considered as a protective genetic marker for pCAD. DPP4 concentrations were lower in the diabetic pCAD patients, and the rs17574GG carriers had the lowest protein levels

Regímenes de bienestar y políticas sectoriales en América Latina

Este libro presenta un resumen de los 15 trabajos emanados del Workshop, en el cual participaron 29 académicos de instituciones de México, España, Argentina y Venezuela. Los autores reflexionan sobre los problemas que ha enfrentado el débil Estado de bienestar en la región, los alcances y limitaciones de la política social de combate a la pobreza, así como las debilidades de los sistemas educativo y de salud en lo que respecta a la resolución de los conflictos sociales que están obligados a atender.Secretaría de Educación Pública - Subsecretaría de Educación Superior - Dirección de Educación Superior Universitaria. Convenio con la SEP: 2017-15-001-017