395 research outputs found
Desarrollo productivo familiar y comunitario desde la Unión de Campesinos Organizados de la cuenca de San Dionisio (U.C.O.S.D) en las comunidades Susulí Central y El Zarzal durante el año 2016
La presente investigación muestra el desarrollo a escala humana, la implementación de una administración la cual llamaremos “planificación” para el desarrollo económico de cada familia y las ECAS dirigidas por el programa de CRS en conjunto con ayuda de la UNAN. La metodología utilizada fue la investigación acción participativa (IAP), los participantes para llevar acabo los dos primeros objetivos fueron los socios de las comunidades Susulí Central y el Zarzal, y para el tercer objetivo se tomaron a 10 productores. Mediante un estudio de caso con técnicas de asambleas comunitarias con informantes claves, el estudio se realizó en tres fases, la primera identificar las transformaciones en el desarrollo a escala humana propuesta por Max-Neef, la segunda un acompañamiento a la planificación productividad, familiar y comunitaria y en la tercera facilitar escuelas de campo del proyecto ASA (Agricultura, Suelo y Agua). Se obtuvo que la UCOSD está contribuyendo al desarrollo a escala humana, se reconocen las carencias y potencialidades. El propósito está en reconocer que los campesinos son los generadores de la riqueza del país y los protagonistas para lograr el desarrollo. Se desarrollaron nuevos formatos de planificación para evaluar la productividad de cada productor y que la organización contara con su propia base de datos, se cumplió el papel de promotores del proyecto ASA implementado entre UNAN y CRS, cuyos resultados fueron tanto beneficiosos como para productores y experiencia para los estudiante
Assessment in cattle under laboratory conditions of drug-resistance of Trypanosoma congolense field isolates from Zambêzia Province, Mozambique
CHEYLETIELLA PARVOSITIVORAX: PRIMEIRO REGISTO DE UMA INFESTAÇÃO EM COELHOS EM MOÇAMBIQUE
Introdução: Os ácaros do género Cheyletiella pertencem à Ordem Prostigmata, Família Cheyletidae. Três espécies são descritas nos animais domésticos nomeadamente, C.yasguri em cães, C. blakei em gatos e C. parasitivorax em coelhos (Taylor et al., 2007). Estas têm distribuição mundial e são consideradas zoonóticas, podendo infectar humanos quando em contacto directo com algum portador (Soulsby, 1982). Os ácaros deste género vivem permanentemente nos pelos, aproximando-se da pele apenas para se alimentarem. As fêmeas durante a ovopostura colam os aos pêlos. Destes eclodem as larvas, que passam por dois estádios ninfais antes de atingirem a fase adulta. O ciclo completo dura aproximadamente duas semanas
Objectivos: O presente poster tem como objectivos reportar a ocorrência da Cheyletiella parasitivorax em Moçambique, contribuir com elementos que permitam o correcto diagnóstico deste ácaro e alertar para a importância zoonótica deste parasita.
Materiais e Métodos: Foram colhidas amostras de pelo e raspagens cutâneas de coelhos com sinais de alopécia, descamação e prurido. As amostras foram processadas pelo método de clarificação com lactofenol de Amman
Resultados: Foram observados ácaros que apresentavam como principais características morfológicas: forma oval, tamanho aproximado de 400 m, patas grandes, palpos com artículos terminais em forma de garra. As características morfológicas e morfométricas conduziram à sua classificação como Cheyletiella parasitivorax.
Discussão: É a primeira vez que um caso de um coelho parasitado com C. parasitovorax é reportado em Moçambique. Por este facto, é necessário chamar a atenção de todos profissionais de veterinária para o correcto diagnóstico e consequentemente tratamento eficaz desta zoonose
Liberal Arts Micro-Credential: Teaching a Police Officer Reflection, Empathy, and Self-Awareness; Going Beyond Training a Police Officer to Act and React
The current precarious relationship between law enforcement and some marginalized minority communities throughout the United States is quite alarming. The Los Angeles riots of 1992, the Baltimore riots of 2015, and the nationwide riots of 2020 serve as a few relatively recent reminders regarding the potentially explosive relationship between marginalized minority communities and the police. Several social and economic factors contribute to the unrest in some marginalized minority communities, such as unemployment and poorly funded educational systems in many areas in the United States. However, in this study, an emphasis was placed on what measures law enforcement agencies can take to establish respectful and productive relationships with marginalized minority communities. Consequently, this study examined the relationship between complaints against the police and a predominantly minority populated police district. This research examined data in the city of Chicago. This study utilized a quantitative non-experimental correlational research design. This study measured a statistical pattern between increasing complaints against the police and predominantly minority populated police districts. The findings of this study disclosed some intriguing results. It seems there is a strong positive correlation between predominantly minority populated police districts and citizen complaints against the police. Subsequently, this study put forth a solution to mend strained relationships with some marginalized minority communities. Specifically, a liberal arts micro-credential can be a solution. A liberal arts micro-credential can provide officers with the needed tools such as enhanced ethics, morals, compassion, and understanding to properly engage with marginalized minority communities. Such a short-term program can cultivate a more ethical and culturally aware attitude among police officers. Subsequently, police officers are better equipped to empathize with marginalized minority communities and foster a healthy relationship
Tracing the Flock: A Look at the Current State of Duck Raising Industry of Hermosa
The study aimed to describe the state of the Duck Raising Industry in the town of Hermosa, Province of Bataan in the Philippines from the point of view of various stakeholders. The study is a qualitative type of research built on a Phenomenological deductive approach. The researcher used interviews as the main tool in gathering the data needed for the study which focuses on the experiences the participants had in association to duck raising and duck egg processing from Mallard Duck locally known as itik. The study revealed that the duck Raising Industry in the town of Hermosa has been part of the growing up years of the participants and has greatly influenced their choice of livelihood and that the second generation of duck raisers in the family take on the business but is unlikely to be favored by the 3rd generation due to shifting careers and varied interests. The duck raising industry remains to be a good source of income and is appreciated by farmers for the quick daily turn out of profits. It is evident that through knowledge acquired from parents and relatives along with personal experiences, Hermosa duck raisers were able to generate best practices, techniques, and methods in duck raising
Utilización del rastrojo de maíz en la fabricación de ladrillos artesanales para muros no portantes
Se tuvo como problema: ¿De qué manera incide la utilización del rastrojo de maíz
en la fabricación de ladrillos artesanales para muros no portantes?, su objetivo fue:
Evaluar la incidencia de la utilización del rastrojo de maíz en la fabricación de ladrillos
artesanales para muros no portantes, la hipótesis general: La utilización del rastrojo de
maíz incide significativamente en la fabricación de ladrillos artesanales para muros no
portantes. El estudio realizado utilizó un enfoque aplicado del método científico, con un
diseño cuasi-experimental de tipo explicativo. La población de estudio consistió en 50
unidades de ladrillos con rastrojo de maíz. Estos detalles metodológicos son relevantes
para comprender la naturaleza y alcance de la investigación realizada. La evaluación de
la incidencia de la utilización del rastrojo de maíz en la fabricación de ladrillos artesanales
para muros no portantes da como resultado que se tiene una incidencia significativa en la
resistencia a la compresión, peso específico y absorción de la unidad de ladrillo, siendo
el porcentaje de adición óptimo el 5% en función del peso del ladrillo, sin embargo, no
se ha encontrado una incidencia significativa para la resistencia a la flexión, así como, el
alabeo tampoco ha sido afectado
Conselhos escolares: instrumentos de democratização da escola pública
Este trabalho parte de uma preocupação que ronda as escolas públicas e suas equipes gestoras a criação dos Conselhos Escolares e sua atuação, que vem de encontro aos anseios da sociedade que é a democratização do ensino público, haja vista que a LDB 9394/96, já prenunciada desde 2006 a implantação deste mecanismo de atuação dentro das escolas públicas. A Instituição escolar é um ambiente propício para exercitar a cidadania democrática como prática social que visa contribuir para o desenvolvimento do cidadão consciente, autônomo e emancipado, e esta constatação é que impulsiona a atuação dos conselhos. O período escolhido, para a análise foi o ano de 2010, com a atual gestão da escola que vem buscando implantar o Conselho Escolar na Escola Municipal Francisco Gonçalves de Britto, atendendo, além de orientações do MEC, à uma legislação municipal divulgada neste ano. O objetivo principal deste trabalho é descrever e analisar os cadernos do Programa Nacional de Fortalecimento dos Conselhos Escolares do Ministério da Educação da Secretaria de Educação Básica e sua funcionalidade
Functional expression of Trypanosoma congolense pyroglutamyl peptidase type 1 and development of reverse genetics tools.
Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2012.Trypanosoma congolense is a protozoan parasite transmitted by tsetse flies. It causes bovine trypanosomosis, the major disease for livestock in sub-Saharan Africa. Control methods include trypanocidal drugs and vector control, but none is fully satisfactory, due to resistance and environmental issues. A method that would have the greatest impact on controlling the disease is vaccination. However, development of a conventional vaccine has been hampered by the mechanism of antigenic variation, which allows the parasite to evade the host’s immune system.
An alternative strategy in vaccine design is to target the bioactive compounds released by dead and dying trypanosomes. This approach is termed ‘‘anti-disease’’, and does not affect the survival of the parasite but targets the pathogenic factors released by the trypanosomes. The development of a successful anti-disease vaccine necessitates knowledge of all pathogenic factors involved in the disease process. Several macromolecules, primarily peptidases, have been implicated in the pathogenesis of trypanosomosis. Pyroglutamyl peptidase type I (PGP) was shown to be involved in abnormal degradation of thyrotropin- and gonadotropin-releasing hormones in rodents infected with T. brucei, but to date no data are available on the T. congolense PGP.
Molecular cloning and expression in E. coli of the coding sequence of T. congolense PGP, as well as the enzymatic characterisation of the recombinant protein, are reported here, completed by the development of reverse genetics tools for studies of gene function.
A 678 bp PCR fragment covering the complete open reading frame of PGP was cloned and sequenced. The deduced amino acid sequence showed 52% and 29% identity with the T. brucei and Leishmania major enzymes respectively. The catalytic residues Glu, Cys and His described in Bacilus amyloliquefaciens PGP are conserved in the T. congolense sequence. PGP was expressed in bacterial systems as a soluble active, 26 kDa enzyme. The recombinant enzyme showed activity specific for the fluorescent substrate pGlu-AMC, with a kcat/Km of 1.11 s-1μM. PGP showed activity in the pH 6.5-10 range, with maximal activity at pH 9.0. The enzyme was strongly inhibited by sulfhydryl-blocking reagents such as iodoacetic acid and iodoacetamide with a kass of 125 M-1 s-1 and 177 M-1 s-1 respectively. Antibodies raised in chickens against the recombinant enzyme allowed the detection of native PGP in both procyclic and bloodstream T.
congolense developmental stages, and displayed complete inhibition of the enzyme in vitro at physiological concentrations. To get insight into the role of PGP in parasite biology and trypanosomosis progression, two types of vectors for reverse genetics studies were developed. For RNA interference, a 400 bp 3′ end segment of the PGP open reading frame was cloned into the plasmid p2T7Ti, that will allow PGP gene down-regulation upon integration into the genome of an engineered tetracycline-inducible strain such as TRUM:29-13. For gene knock-out, several rounds of molecular engineering were carried-out in order to create two plasmid vectors, pGL1184-based (blasticidin resistance) and pGL1217-based (neomycin resistance), each bearing 200 bp-long regions at the 5′ and 3′ ends of the PGP open reading frame. In subsequent studies, taking advantage of the recent advances in culture and transformation of T. congolense, these plasmids will allow the creation of single and double knock-out mutants of PGP
- …
