Evaluation of sweetpotato (Ipomoea batatas (L.) Lam.) germplasm from north-eastern Uganda through a Farmer Participatory Approach

Ugandan farmers grow many landrace sweetpotato varieties, but some of these are relatively low yielding and susceptible to pests. The objective of the present research was to involve farmers in a large-scale assessment of Ugandan farmers’ varieties to rapidly identify those with superior yield performance, pest resistance and consumer acceptance. One hundred sixty distinct farmers’ varieties collected from Lira, Soroti, Katakwi, Kumi and Pallisa Districts of North-eastern Uganda were evaluated in on-station trials. Trials were conducted at two sites (Serere Agricultural and Animal Research Institute and Arapai Agricultural College) in Soroti District in the second rainy season of 1999. Twenty-five farmers from surrounding areas participated in trial harvest at each site. At harvest, fresh storage root yield, foliage yield, and dry matter content were determined by researchers. Farmers observed a number of characteristics and rated each entry with respect to the following variables: general impression, dry matter content, pests, and defects. A strong positive correlation was observed between farmers’ general impression and yield and harvest index in the trials. Farmers selected 10 superior varieties from each trial for further multi-environment, on-station and on-farm trials. Coincidentally, nine of the selected varieties were common to both sites

K(E10), Supergravity and Fermions

We study the fermionic extension of the E10/K(E10) coset model and its relation to eleven-dimensional supergravity. Finite-dimensional spinor representations of the compact subgroup K(E10) of E(10,R) are studied and the supergravity equations are rewritten using the resulting algebraic variables. The canonical bosonic and fermionic constraints are also analysed in this way, and the compatibility of supersymmetry with local K(E10) is investigated. We find that all structures involving A9 levels 0,1 and 2 nicely agree with expectations, and provide many non-trivial consistency checks of the existence of a supersymmetric extension of the E10/K(E10) coset model, as well as a new derivation of the `bosonic dictionary' between supergravity and coset variables. However, there are also definite discrepancies in some terms involving level 3, which suggest the need for an extension of the model to infinite-dimensional faithful representations of the fermionic degrees of freedom.Comment: 50 page

Sugawara-type constraints in hyperbolic coset models

In the conjectured correspondence between supergravity and geodesic models on infinite-dimensional hyperbolic coset spaces, and E10/K(E10) in particular, the constraints play a central role. We present a Sugawara-type construction in terms of the E10 Noether charges that extends these constraints infinitely into the hyperbolic algebra, in contrast to the truncated expressions obtained in arXiv:0709.2691 that involved only finitely many generators. Our extended constraints are associated to an infinite set of roots which are all imaginary, and in fact fill the closed past light-cone of the Lorentzian root lattice. The construction makes crucial use of the E10 Weyl group and of the fact that the E10 model contains both D=11 supergravity and D=10 IIB supergravity. Our extended constraints appear to unite in a remarkable manner the different canonical constraints of these two theories. This construction may also shed new light on the issue of `open constraint algebras' in traditional canonical approaches to gravity.Comment: 49 page

Curvature corrections and Kac-Moody compatibility conditions

We study possible restrictions on the structure of curvature corrections to gravitational theories in the context of their corresponding Kac--Moody algebras, following the initial work on E10 in Class. Quant. Grav. 22 (2005) 2849. We first emphasize that the leading quantum corrections of M-theory can be naturally interpreted in terms of (non-gravity) fundamental weights of E10. We then heuristically explore the extent to which this remark can be generalized to all over-extended algebras by determining which curvature corrections are compatible with their weight structure, and by comparing these curvature terms with known results on the quantum corrections for the corresponding gravitational theories.Comment: 27 page

E10 and SO(9,9) invariant supergravity

We show that (massive) D=10 type IIA supergravity possesses a hidden rigid SO(9,9) symmetry and a hidden local SO(9) x SO(9) symmetry upon dimensional reduction to one (time-like) dimension. We explicitly construct the associated locally supersymmetric Lagrangian in one dimension, and show that its bosonic sector, including the mass term, can be equivalently described by a truncation of an E10/K(E10) non-linear sigma-model to the level \ell<=2 sector in a decomposition of E10 under its so(9,9) subalgebra. This decomposition is presented up to level 10, and the even and odd level sectors are identified tentatively with the Neveu--Schwarz and Ramond sectors, respectively. Further truncation to the level \ell=0 sector yields a model related to the reduction of D=10 type I supergravity. The hyperbolic Kac--Moody algebra DE10, associated to the latter, is shown to be a proper subalgebra of E10, in accord with the embedding of type I into type IIA supergravity. The corresponding decomposition of DE10 under so(9,9) is presented up to level 5.Comment: 1+39 pages LaTeX2e, 2 figures, 2 tables, extended tables obtainable by downloading sourc

An E9 multiplet of BPS states

We construct an infinite E9 multiplet of BPS states for 11D supergravity. For each positive real root of E9 we obtain a BPS solution of 11D supergravity, or of its exotic counterparts, depending on two non-compact transverse space variables. All these solutions are related by U-dualities realised via E9 Weyl transformations in the regular embedding of E9 in E10, E10 in E11. In this way we recover the basic BPS solutions, namely the KK-wave, the M2 brane, the M5 brane and the KK6-monopole, as well as other solutions admitting eight longitudinal space dimensions. A novel technique of combining Weyl reflexions with compensating transformations allows the construction of many new BPS solutions, each of which can be mapped to a solution of a dual effective action of gravity coupled to a certain higher rank tensor field. For real roots of E10 which are not roots of E9, we obtain additional BPS solutions transcending 11D supergravity (as exemplified by the lowest level solution corresponding to the M9 brane). The relation between the dual formulation and the one in terms of the original 11D supergravity fields has significance beyond the realm of BPS solutions. We establish the link with the Geroch group of general relativity, and explain how the E9 duality transformations generalize the standard Hodge dualities to an infinite set of `non-closing dualities'.Comment: 76 pages, 6 figure

Development of an eight-band theory for quantum-dot heterostructures

We derive a nonsymmetrized 8-band effective-mass Hamiltonian for quantum-dot heterostructures (QDHs) in Burt's envelope-function representation. The 8x8 radial Hamiltonian and the boundary conditions for the Schroedinger equation are obtained for spherical QDHs. Boundary conditions for symmetrized and nonsymmetrized radial Hamiltonians are compared with each other and with connection rules that are commonly used to match the wave functions found from the bulk kp Hamiltonians of two adjacent materials. Electron and hole energy spectra in three spherical QDHs: HgS/CdS, InAs/GaAs, and GaAs/AlAs are calculated as a function of the quantum dot radius within the approximate symmetrized and exact nonsymmetrized 8x8 models. The parameters of dissymmetry are shown to influence the energy levels and the wave functions of an electron and a hole and, consequently, the energies of both intraband and interband transitions.Comment: 36 pages, 10 figures, E-mail addresses: [email protected], [email protected]

K(E9) from K(E10)

We analyse the M-theoretic generalisation of the tangent space structure group after reduction of the D=11 supergravity theory to two space-time dimensions in the context of hidden Kac-Moody symmetries. The action of the resulting infinite-dimensional `R symmetry' group K(E9) on certain unfaithful, finite-dimensional spinor representations inherited from K(E10) is studied. We explain in detail how these representations are related to certain finite codimension ideals within K(E9), which we exhibit explicitly, and how the known, as well as new finite-dimensional `generalised holonomy groups' arise as quotients of K(E9) by these ideals. In terms of the loop algebra realisations of E9 and K(E9) on the fields of maximal supergravity in two space-time dimensions, these quotients are shown to correspond to (generalised) evaluation maps, in agreement with previous results of Nicolai and Samtleben (hep-th/0407055). The outstanding question is now whether the related unfaithful representations of K(E10) can be understood in a similar way.Comment: 35 pages; v2: References added; v3: Author, one reference and two appendices added. Extended results in sections 3.2 and 4.

ISG15 and ISGylation is required for pancreatic cancer stem cell mitophagy and metabolic plasticity

Pancreatic cancer stem cells (PaCSCs) drive pancreatic cancer tumorigenesis, chemoresistance and metastasis. While eliminating this subpopulation of cells would theoretically result in tumor eradication, PaCSCs are extremely plastic and can successfully adapt to targeted therapies. In this study, we demonstrate that PaCSCs increase expression of interferon-stimulated gene 15 (ISG15) and protein ISGylation, which are essential for maintaining their metabolic plasticity. CRISPR-mediated ISG15 genomic editing reduces overall ISGylation, impairing PaCSCs self-renewal and their in vivo tumorigenic capacity. At the molecular level, ISG15 loss results in decreased mitochondrial ISGylation concomitant with increased accumulation of dysfunctional mitochondria, reduced oxidative phosphorylation (OXPHOS) and impaired mitophagy. Importantly, disruption in mitochondrial metabolism affects PaCSC metabolic plasticity, making them susceptible to prolonged inhibition with metformin in vivo. Thus, ISGylation is critical for optimal and efficient OXPHOS by ensuring the recycling of dysfunctional mitochondria, and when absent, a dysregulation in mitophagy occurs that negatively impacts PaCSC stemness

Exploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells

Pancreatic ductal adenocarcinoma (PDAC), the fourth leading cause of cancer death, has a 5-year survival rate of approximately 7–9%. The ineffectiveness of anti-PDAC therapies is believed to be due to the existence of a subpopulation of tumor cells known as cancer stem cells (CSCs), which are functionally plastic, and have exclusive tumorigenic, chemoresistant and metastatic capacities. Herein, we describe a 2D in vitro system for long-term enrichment of pancreatic CSCs that is amenable to biological and CSC-specific studies. By changing the carbon source from glucose to galactose in vitro, we force PDAC cells to utilize OXPHOS, resulting in enrichment of CSCs defined by increased CSC biomarker and pluripotency gene expression, greater tumorigenic potential, induced but reversible quiescence, increased OXPHOS activity, enhanced invasiveness, and upregulated immune evasion properties. This CSC enrichment method can facilitate the discovery of new CSC-specific hallmarks for future development into targets for PDAC-based therapies