Construction and Testing of a Biological Breathalyzer

The ultimate goal of this research was the construction of a biological breathalyzer using methods of synthetic biology. The metabolic pathways of Pichia taxa were utilized for this research. This yeast is able to metabolize both ethanol and methanol present in the environment. The first known by-product of methanol metabolism is the AO enzyme from the AOX gene. When both ethanol and methanol are present, the yeast preferentially metabolizes ethanol and the AOX gene is not expressed. By fusing the AOX gene promoter with a fluorescence protein gene, the expression of the AOX gene may be visually detected. When the organism is supplied with both ethanol and methanol, the amount of time before fluorescence will correspond to the amount of ethanol fed to the cell. In this way, the concentration of ethanol can be determined

Biological Breathalyzer

The aim of this research is the construction of a biological breathalyzer through synthetic biology, specifically through use of the metabolic pathways of a species of the Pichia taxa. The yeast utilized is able to metabolize both ethanol and methanol. However, when both ethanol and methanol are present, the yeast prefers to metabolize ethanol such that an AOX gene is not expressed because the first known by-product of methanol metabolism is the AO enzyme from the AOX gene. The AOX gene promoter is fused with a fluorescence protein gene so expression of the AOX gene can be visually detected. When the cell is supplied with both ethanol and methanol, the amount of time before fluorescence will correspond to the amount of ethanol given to the cell. In this way, the concentration of ethanol can be determined

Dating and morpho-stratigraphy of uplifted marine terraces in the Makran subduction zone (Iran)

The western part of the Makran subduction zone (Iran) is currently experiencing active surface uplift, as attested by the presence of emerged marine terraces along the coast. To better understand the uplift recorded by these terraces, we investigated seven localities along the Iranian Makran and we performed radiocarbon, 230Th∕U and optically stimulated luminescence (OSL) dating of the layers of marine sediments deposited on top of the terraces. This enabled us to correlate the terraces regionally and to assign them to different Quaternary sea-level highstands. Our results show east–west variations in surface uplift rates mostly between 0.05 and 1.2 mm yr−1. We detected a region of anomalously high uplift rate, where two MIS 3 terraces are emerged, but we are uncertain how to interpret these results in a geologically coherent context. Although it is presently not clear whether the uplift of the terraces is linked to the occurrence of large megathrust earthquakes, our results highlight rapid surface uplift for a subduction zone context and heterogeneous accumulation of deformation in the overriding plate

Adjunctive primary stenting of Zenith endograft limbs during endovascular abdominal aortic aneurysm repair: Implications for limb patency

ObjectiveEndograft limb occlusion is an infrequent but serious complication of endovascular abdominal aortic aneurysm (AAA) repair. The insertion of additional stents within the endograft limb may prevent future occlusion. This study evaluates limb patency with and without adjunctive stenting of endograft limbs at the time of endovascular AAA repair.MethodsWe performed a retrospective review of 248 patients who underwent endovascular abdominal aortic aneurysm repair with the Zenith AAA endovascular graft between 1999 and 2004. Among these patients, two groups were identified: 64 patients with adjunctive stents placed in 85 limbs and 184 patients without additional bare stent placement in endograft limbs at the time of endovascular AAA repair.ResultsWomen comprised 23% of stented and 11% of unstented patients (P = .02). The mean length of follow-up in the stented and unstented groups was 2.0 years. There were 13 instances of limb thrombosis in 13 patients (5.2% of patients, 2.7% of limbs), all in the unstented group. No limb occlusions occurred in the presence of adjunctive bare metal stents. Seventy-three percent of the occlusions occurred ≤6 months of endovascular AAA repair. Two patients (15%) had no symptoms of lower-extremity ischemia despite graft limb occlusion and did not undergo intervention. The others underwent thrombectomy (n = 2), thrombectomy with bare stent placement (n = 3), femoral-femoral bypass (n = 4), thrombolysis (n = 1), and thrombolysis with bare stent placement (n = 1). Of the seven who underwent thrombectomy or thrombolysis, three had no additional stents placed at the secondary procedure, and two of these three went on to rethrombose. By life-table analysis, primary patency at 3 years in the stented and nonstented limbs was 100% ± 0% and 94% ± 3%, respectively (P = .05).ConclusionsThe intraoperative insertion of additional bare metal stents appeared to eliminate the risk of thrombosis and was without complication. Of the 85 stented limbs in this series, not one occluded. The overall rate of limb thrombosis was low, with most limb occlusions occurring ≤6 months of stent-graft insertion, and would probably have been even lower had we been able to identify all high-risk cases for prophylactic adjunctive stenting. Limb occlusion denotes an underlying problem with the graft, which if left untreated after thrombectomy or thrombolysis will lead to rethrombosis. Postoperative imaging was of little value in detecting impending limb occlusion. Based on these findings, we believe one should identify and stent any limbs that appear to be at risk for thrombosis, but this study lacks the data to predict which limbs need stenting

Test of variational approximation for phi4phi^4 quantum chain by Monte Carlo simulation

We report results of a Monte Carlo simulation of the $\phi^4$ quantum chain. In order to enhance the efficiency of the simulation we combine multigrid simulation techniques with a refined discretization scheme. The resulting accuracy of our data allows for a significant test of an analytical approximation based on a variational ansatz. While the variational approximation is well reproduced for a large range of parameters we find significant deviations for low temperatures and large couplings.Comment: 12 pp. Latex + 3 figures as uuencoded compressed tar file, accepted for publication in Phys. Lett.

Exploring the Trophic Spectrum: Placing Mixoplankton Into Marine Protist Communities of the Southern North Sea

While traditional microplankton community assessments focus primarily on phytoplankton and protozooplankton, the last decade has witnessed a growing recognition of photo-phago mixotrophy (performed by mixoplankton) as an important nutritional route among plankton. However, the trophic classification of plankton and subsequent analysis of the trophic composition of plankton communities is often subjected to the historical dichotomy. We circumvented this historical dichotomy by employing a 24 year-long time series on abiotic and protist data to explore the trophic composition of protist communities in the Southern North Sea. In total, we studied three different classifications. Classification A employed our current knowledge by labeling only taxa documented to be mixoplankton as such. In a first trophic proposal (classification B), documented mixoplankton and all phototrophic taxa (except for diatoms, cyanobacteria, and colonial Phaeocystis) were classified as mixoplankton. In a second trophic proposal (classification C), documented mixoplankton as well as motile, phototrophic taxa associated in a principle component analysis with documented mixoplankton were classified as mixoplankton. In all three classifications, mixoplankton occurred most in the inorganic nutrient-depleted, seasonally stratified environments. While classification A was still subjected to the traditional dichotomy and underestimated the amount of mixoplankton, our results indicate that classification B overestimated the amount of mixoplankton. Classification C combined knowledge gained from the other two classifications and resulted in a plausible trophic composition of the protist community. Using results of classification C, our study provides a list of potential unrecognized mixoplankton in the Southern North Sea. Furthermore, our study suggests that low turbidity and the maturity of an ecosystem, quantified using a newly proposed index of ecosystem maturity (ratio of organic to total nitrogen), provide an indication on the relevance of mixoplankton in marine protist communities